Abstract：
:The disease melioidosis, caused by the soil bacteria Burkholderia pseudomallei, often manifests as acute septicemia with high fatality. Glycogen synthase kinase-3β (GSK3β) plays a key role during the inflammatory response induced by bacteria. We used a murine model of acute melioidosis to investigate the effects of LiCl, a GSK3 inhibitor on experimental animal survivability as well as TNF-α, IL-1β, IFN-γ, IL-10 and IL-1Ra cytokine levels in blood, lung, liver and spleen of B. pseudomallei-infected mice. Our results showed that administration of 100 μg/g LiCl improved survivability of mice infected with 5 X LD50 of B. pseudomallei. Bacterial counts in spleen, liver and lungs of infected mice administered with LiCl were lower than non-treated controls. Our data also revealed that GSK3β is phosphorylated in the spleen, liver and lung of animals infected with B. pseudomallei. However in infected animals administered with LiCl, higher levels of pGSK3 were detected in the organs. Levels of proinflammatory cytokines (TNF-α, IL-1β and IFN-γ) and anti-inflammatory cytokines (IL-10 and IL-1Ra) in sera and organs tested were elevated significantly following B. pseudomallei infection. With GSK3β inhibition, pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β) were significantly decreased in all the samples tested whilst the levels of anti-inflammatory cytokines, IL-10 (spleen and lung) and IL-1Ra (spleen, liver and sera) were further elevated. This study represents the first report implicating GSK3β in the modulation of cytokine production during B. pseudomallei infection thus reiterating the important role of GSK3β in the inflammatory response caused by bacterial pathogens.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Tay TF,Maheran M,Too SL,Hasidah MS,Ismail G,Embi Nsubject
Has Abstractpub_date
2012-12-01 00:00:00pages
551-67issue
4eissn
0127-5720issn
2521-9855journal_volume
29pub_type
杂志文章abstract：:This study assessed the effectiveness of a biorational control approach using 2% hydramethylnon gel bait on German cockroaches, Blattella germanica (L.) in some residential and hospital buildings in South Western Iran. In total, three buildings consisting of 150 apartment units and 101 hospital units were monitored we...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：:A total of 29 female black flies were captured by a hand net as they swarmed around humans in Tam Dao National Park, Vinh Phuc Province, Vietnam. They included one species of the subgenus Gomphostilbia (Simulium (Gomphostilbia) asakoae Takaoka & Davies) and five species of the subgenus Simulium, of which one species i...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:A study was conducted to evaluate the infection status of Blastocystis hominis in children from four public schools in Phuttamonthon district, Nakhon Pathom province, Thailand during November to December 2004. A total of 814 faecal specimens were used for B. hominis cultivation using Jones' medium. Mixed infections wi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:The majority of amoeba infection are asymptomatic, but clinically intestinal amoebiasis or extra intestinal amoebiasis may result. Genital amoebiasis is very rare manifestation of extra intestial amoebiasis, but a case of amoebic scrotal abscess, seen in Surabaya. The invasive form of Entamoeba histolytica trophozoite...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:Prior to the implementation of Haemophilus influenzae type b vaccination worldwide, H. influenzae has been one of the main causative agents of community acquired pneumonia and meningitis in children. Due to the lack of information on the characteristics of the H. influenzae isolates that have previously been col...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:Understanding the genetic diversity, extent and distribution of variant forms of Plasmodium vivax parasites is crucial in the development of effective control measures and in Orissa, a hyperendemic state in the eastern part of India, the polymorphic nature of P. vivax isolates is largely lacking. The result of the stu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:The emergence of bone marrow micronucleated polychromatic erythrocytes (MN-PCE) in rats experimentally infected with Trypanosoma brucei brucei was examined in order to understand the bone marrow effects in trypanosomiasis infection. Bone marrow was collected for micronucleus assay while blood samples were collected fr...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：:This paper presents investigation of lungworm disease outbreaks that is based on retrospective examination of cases recorded between 1994 and 2000 on a government beef cattle breeding centre in the state of Pahang, peninsular Malaysia. The breed of cattle on the centre was Nelore and the mean population over a 7-year ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:The objective of this work is to evaluate the usefulness of a standardized clinical classification of hydroceles in lymphatic filariasis endemic countries to guide their surgical management. 64 patients with hydroceles were operated in 2009-2010, in Level II hospitals (WHO classification), during two visits to F...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:One hundred and sixty eight rats were trapped from the National Service Training Centres (NSTC) in Kelantan and Terengganu from October 2008 to May 2009. Microscopic agglutination test (MAT) was performed to detect the presence of agglutinating antibodies to Leptospira among the rats caught. All the MAT positive rats ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:This study explored the efficacy of Toxorhynchites splendens, predator of Aedes albopictus as a biocontrol agent. There was a negative correlation between Ae. albopictus larval population and Tx. splendens larval population in ovitraps (r=-0.287, R²=0.0821). The correlation is higher between the mean number of Ae. alb...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:Limited data is available on the prevalence of parasitic infections among the island communities in Malaysia with most studies performed between 1960s-1980s. This study was conducted to determine the current prevalence status of parasitic infections among communities living in Pangkor Island Peninsular Malaysia. A tot...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:Studies were carried out on the residual efficacy of Bacillus thuringiensis H-14 (water dispersible granule, VectoBac ABG 6511) as direct application in the control of Aedes larvae in the field. Field Aedes sp populations in the earthen and glass jars were predetermined before initiation of the trial. On confirmation ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
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journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:A survey of cockroach fauna was carried out on the 3 inhabited islands of the Ogasawara chain island of Japan, namely, Chichijima island, Hahajima island and Iwo island. Seven species, namely, Periplaneta americana (Linnaeus, 1758), Periplaneta australasiae (Fabricius, 1775), Blattella lituricollis (Walker, 1868), Ony...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:In the present study, a total of 1,235 porcine serum samples were collected from 9 counties in Jilin Province (40°52'~46°18'N,121°38'~131°19'E), northeastern China from August to October 2013, and the seroprevalence of Toxoplasma gondii infection was tested by indirect haemagglutination assay (IHA). The results showed...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:Due to the disadvantages of human and equine rabies immunoglobulin, it is necessary to develop a substitute for HRIG and ERIG, especially for those people living in the developing countries. Because of higher affinity and lower immunogenicity of rabbit's immunoglobulins, anti-rabies immunoglobulins specific to rabies ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:Field bioefficacy of residual-sprayed deltamethrin against Aedes vectors was evaluated in an urban residential area in Kuala Lumpur. The trial area consisted of single storey wood-brick houses and a block of flat. The houses were treated with outdoor residual spraying while the flat was used as an untreated control. I...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Escherichia coli (E. coli) strains are the major cause of urinary tract infections (UTI) and belong to the large group of extra-intestinal pathogenic E. coli. The purposes of this study were to determine the antibiotic resistance profile, virulence genes and phylogenetic background of E. coli isolates from UTI cases. ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:During a forensic entomological study conducted in a palm oil plantation in Tg.Sepat, Selangor in September 2007, a spider (Arachnida), Oxyopes sp. (Oxyopidae) was found to predate on a calliphorid fly (Chrysomya rufifacies). The female spider laid a silk thread, or "drag line", behind it as it moved. This spider bite...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-04-01 00:00:00
abstract：:This is the first report of Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae) on a human corpse discovered in a high-rise building in Malaysia. On 5 March 2008, a decomposing body of an adult female was found on the top floor of a thirteen-story building in Kuala Lumpur, Malaysia. Her body was colonized by S. nu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts o...
journal_title：Tropical biomedicine
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:This study was conducted to investigate the low prevalence of Dirofilaria immitis in dogs in Johor Bahru as reported by veterinary practitioners, using wet blood mount, Knott's Concentration Test and two heartworm antigen test kits (IDEXX Canine SNAP® 4Dx and RapiGEN®). This study also compared the two test kits...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:Plasmodim vivax malaria accounts for more than 90% of malaria cases in Sri Lanka. There is limited information on the genetic heterogeneity of P. vivax in endemic areas of the country. Here we have assessed the potential of two P. vivax genes as genetic markers for their use in genotyping parasites collected from the ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:The determination of parasitemia in the diagnosis of malaria is a routine practice because it assists the selection of treatment. The techniques used for estimating parasitemia are based on leukocyte counts or on a fixed volume of blood examined in a microscopic field. This study evaluated the concordance between para...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：:Crab is an important source of food allergen. Tropomyosin represents the main crab allergen and is responsible for IgE cross-reactivity between various species of crustaceans. Recently, other new crab allergens including arginine kinase have been identified. However, information on allergens of the local Portuni...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:Entamoeba histolytica causes amoebic diarrhoea, colitis and liver abscess (ALA). Diagnosis of ALA is difficult, as most patients do not have simultaneous intestinal amoebic infection. At Hospital Universiti Sains Malaysia (HUSM), diagnosis of ALA relies on a combination of clinical findings, ultrasound examination of ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:Dengue serotype surveillance is important as any changes in serotype distribution may result in an outbreak or increase in severe dengue cases. This study aimed to determine circulating dengue serotypes in two hospitals in Selangor. Serum samples were collected from patients admitted for dengue at these two major publ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:Analysis on fly artifacts produced by forensically important blowfly, Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae), revealed several unique patterns. They can be divided into fecal spots, regurgitation spots and swiping stains. The characteristics of fecal spots are round with three distinct levels of pig...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-04-01 00:00:00
abstract：:The adulticidal activity of methanol extracts from three Malaysian plants namely Acorus calamus Linn., Litsea elliptica Blume and Piper aduncum Linn. against adult of Aedes aegypti (L.) were studied. Standard WHO bioassay tests were used to evaluate the effectiveness of these plant extracts. The hexane fraction from m...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
