Abstract：
:Entamoeba histolytica causes amoebic diarrhoea, colitis and liver abscess (ALA). Diagnosis of ALA is difficult, as most patients do not have simultaneous intestinal amoebic infection. At Hospital Universiti Sains Malaysia (HUSM), diagnosis of ALA relies on a combination of clinical findings, ultrasound examination of the liver and serodiagnosis using a commercial kit. In this study, two in-house indirect ELISAs were developed and evaluated. One of the in-house assays utilises E. histolytica crude soluble antigen (CSA) to detect serum IgG specific to the parasite whereas the other uses E. histolytica ether extract antigen (EEA). Preparation of CSA requires a sonicator to lyse the amoeba whereas EEA was prepared by chemically solubilizing the trophozoites. Based on the cut-off value of mean optical density + 3SD, CSA-ELISA showed 100% (24/24) sensitivity and 93.33% (210/225) specificity; while EEA-ELISA showed 91.67% (22/24) sensitivity and 95.11% (214/225) specificity. In conclusion, both the in-house indirect ELISAs were found to be efficacious for diagnosis of ALA; and the EEA is easier to prepare than the commonly used CSA.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Tan ZN,Wong WK,Noordin R,Zeehaida M,Olivos GA,Lim BHsubject
Has Abstractpub_date
2013-06-01 00:00:00pages
250-6issue
2eissn
0127-5720issn
2521-9855journal_volume
30pub_type
杂志文章abstract：:The majority of amoeba infection are asymptomatic, but clinically intestinal amoebiasis or extra intestinal amoebiasis may result. Genital amoebiasis is very rare manifestation of extra intestial amoebiasis, but a case of amoebic scrotal abscess, seen in Surabaya. The invasive form of Entamoeba histolytica trophozoite...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:Plasmodim vivax malaria accounts for more than 90% of malaria cases in Sri Lanka. There is limited information on the genetic heterogeneity of P. vivax in endemic areas of the country. Here we have assessed the potential of two P. vivax genes as genetic markers for their use in genotyping parasites collected from the ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:A total of 105 blood samples from healthy horses from different stables in Yazd province, center of Iran, were examined for the presence of Theileria equi infection using parasitological and molecular methods. Out of the 105 samples, the parasitological method detected T. equi infection in 5 (4.76%) cases while the PC...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:Poultry farming not only provides high nutritious food but also creates employment opportunity for rural masses. Documented evidences elaborates that helminth parasitism is most deciduous problem of chickens especially in developing world. Ascaridia (A.) galli, a nematode of small intestine, has been considered as the...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:Plaque assay procedure was carried out to determine the infectivity titration of dengue virus using Aedes albopictus C6/36 mosquito cell line. Cells were seeded in 6 wells plates and incubated until monolayer C6/36 cell lines were formed. Each well was exposed to a different dilution of dengue infected culture fluid, ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Cryptosporidiosis is a zoonotic protozoan disease of worldwide distribution, affecting a wide range of vertebrate hosts. Most data on the biology, distribution pattern, pathology and prevalence of cryptosporidial infection in farm animals is restricted to cattle, sheep and goats. Limited data is available in other dom...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:Aim of present study was to screen medicinal plants for flukicidal activity in vitro to develop alternative sources of treatment for trematodes infection. For this purpose, crude methanolic extracts (CME) of Cymbopogn jwarancusa and Conyza canadensis were prepared and live adult flukes viz; Fasciola gigantica, and Par...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:Studies were carried out on the residual efficacy of Bacillus thuringiensis H-14 (water dispersible granule, VectoBac ABG 6511) as direct application in the control of Aedes larvae in the field. Field Aedes sp populations in the earthen and glass jars were predetermined before initiation of the trial. On confirmation ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:A human corpse at an advanced stage of decomposition was found in a house in the residential area of Bukit Mertajam, Penang, Malaysia. Entomological specimens were collected during the post-mortem and the live specimens were subsequently reared at room temperature. The time of death was estimated to have been 14 days ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2009-04-01 00:00:00
abstract：:During 2008-2009 a total of 67 individuals of rodents, Tetera indica, Meriones hurrianae, Meriones libycus and Gerbillus nanus were trapped in three areas, Bampor, Daman and Qasre Qand from Iranshahr and Nikshahr districts. There is a significant difference between comparative abundance of four species (P<0.05). A tot...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 - 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The m...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:This study assessed the effectiveness of a biorational control approach using 2% hydramethylnon gel bait on German cockroaches, Blattella germanica (L.) in some residential and hospital buildings in South Western Iran. In total, three buildings consisting of 150 apartment units and 101 hospital units were monitored we...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：:Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts o...
journal_title：Tropical biomedicine
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:Using standard WHO methodology, this study investigated the susceptibility of 4(th) instar Aedes aegypti (L) and Culex annulirostris (Skuse) larvae to three extracts from Callitris glaucophylla (J. Thompson & L. Johnson) (1: steam distillation extract, 2: liquefied refrigerant gas extract, and 3: methanol reflux extra...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:Limited data is available on the prevalence of parasitic infections among the island communities in Malaysia with most studies performed between 1960s-1980s. This study was conducted to determine the current prevalence status of parasitic infections among communities living in Pangkor Island Peninsular Malaysia. A tot...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:Understanding the genetic diversity, extent and distribution of variant forms of Plasmodium vivax parasites is crucial in the development of effective control measures and in Orissa, a hyperendemic state in the eastern part of India, the polymorphic nature of P. vivax isolates is largely lacking. The result of the stu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:One hundred and sixty eight rats were trapped from the National Service Training Centres (NSTC) in Kelantan and Terengganu from October 2008 to May 2009. Microscopic agglutination test (MAT) was performed to detect the presence of agglutinating antibodies to Leptospira among the rats caught. All the MAT positive rats ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:Signal fly, Scholastes sp. (Diptera: Platystomatidae) was observed associated with animal carcasses in Malaysia. The first observation was on a monkey carcass, which was killed by using a handgun and immediately placed in a forested area in Gombak, Selangor while the second observation was on a pig that died of natura...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:Piophila casei (Linnaeus) (Diptera: Piophilidae) is reported from human cadavers in two separate forensic cases for the first time in Malaysia. Both bodies were found indoors. The first case, was that of a male of unknown nationality and age and also contained maggots of the muscid Ophyra spinigera (Stein). The second...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:This is the first report of Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae) on a human corpse discovered in a high-rise building in Malaysia. On 5 March 2008, a decomposing body of an adult female was found on the top floor of a thirteen-story building in Kuala Lumpur, Malaysia. Her body was colonized by S. nu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:This study explored the efficacy of Toxorhynchites splendens, predator of Aedes albopictus as a biocontrol agent. There was a negative correlation between Ae. albopictus larval population and Tx. splendens larval population in ovitraps (r=-0.287, R²=0.0821). The correlation is higher between the mean number of Ae. alb...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:Strongyloides stercoralis is a widespread, soil-transmitted helminth affecting humans. Autoinfection occurs in S. stercoralis infection and this leads to a continuous build-up of worm burden in human host. This may lead to hyperinfection syndrome which has the potential to cause serious life-threatening disease especi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:This study was conducted to investigate the low prevalence of Dirofilaria immitis in dogs in Johor Bahru as reported by veterinary practitioners, using wet blood mount, Knott's Concentration Test and two heartworm antigen test kits (IDEXX Canine SNAP® 4Dx and RapiGEN®). This study also compared the two test kits...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:The emergence of bone marrow micronucleated polychromatic erythrocytes (MN-PCE) in rats experimentally infected with Trypanosoma brucei brucei was examined in order to understand the bone marrow effects in trypanosomiasis infection. Bone marrow was collected for micronucleus assay while blood samples were collected fr...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-06-01 00:00:00
abstract：:Routine surveillance on resistant status of field mosquito populations is important to implement suitable strategies in order to prevent pest outbreaks. WHO test kit bioassay is the most frequent bioassay used to investigate the susceptibility status of field-collected mosquitoes, as it is relatively convenient ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:The adulticidal activity of methanol extracts from three Malaysian plants namely Acorus calamus Linn., Litsea elliptica Blume and Piper aduncum Linn. against adult of Aedes aegypti (L.) were studied. Standard WHO bioassay tests were used to evaluate the effectiveness of these plant extracts. The hexane fraction from m...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：:Dengue serotype surveillance is important as any changes in serotype distribution may result in an outbreak or increase in severe dengue cases. This study aimed to determine circulating dengue serotypes in two hospitals in Selangor. Serum samples were collected from patients admitted for dengue at these two major publ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:The objective of this work is to evaluate the usefulness of a standardized clinical classification of hydroceles in lymphatic filariasis endemic countries to guide their surgical management. 64 patients with hydroceles were operated in 2009-2010, in Level II hospitals (WHO classification), during two visits to F...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:In this study, 13 weeks (October to December 2012) of ovitrap surveillance was conducted in two suburban residential areas in Kampar town, Perak. A total of 17,310 Aedes mosquitoes were found in Taman Kampar Jaya, whereas Taman Juloong recorded a higher number at 19,042. Less than 1% of these were identified as Aedes ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:In this case series report we aim to report a Disseminated Intravascular Coagulation (DIC)-like syndrome associated with Hemiscorpius lepturus sting in 4 individuals and a novel management protocol for this life-threatening condition that comprised partial exchange transfusion in severe scorpionism. ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
