Abstract：
:Plaque assay procedure was carried out to determine the infectivity titration of dengue virus using Aedes albopictus C6/36 mosquito cell line. Cells were seeded in 6 wells plates and incubated until monolayer C6/36 cell lines were formed. Each well was exposed to a different dilution of dengue infected culture fluid, followed by addition of first and second overlay medium. Cells were observed under an inverted microscope and plaque staining was carried out. The results showed that countable plaques were not achieved and plaques formed were restricted to the central section due to the significant partial lysis of the C6/36 cells in the plates. However, the plaque assay technique using C6/36 cell line reported here appears to be promising and merits further detailed studies.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Zamree I,Drakes N,Rohani A,Lee HLsubject
Has Abstractpub_date
2005-12-01 00:00:00pages
217-9issue
2eissn
0127-5720issn
2521-9855journal_volume
22pub_type
杂志文章abstract：:Mosquitoes transmit serious human diseases, causing millions of deaths every year. Use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. Insecticides of botanical origin may serve as suitable alternative bi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-08-01 00:00:00
abstract：:One hundred and sixty eight rats were trapped from the National Service Training Centres (NSTC) in Kelantan and Terengganu from October 2008 to May 2009. Microscopic agglutination test (MAT) was performed to detect the presence of agglutinating antibodies to Leptospira among the rats caught. All the MAT positive rats ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:This paper reports an outbreak of trypanosomiasis due to Trypanosoma evansi in Java deer (Cervus timorensis) on a government deer farm in Lenggong, Perak. Seventeen adult female Java deer were found dead within a week. Symptoms of dullness, inappetence, anaemia, anorexia, respiratory distress and recumbency were seen ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：:A survey of cockroach fauna was carried out on the 3 inhabited islands of the Ogasawara chain island of Japan, namely, Chichijima island, Hahajima island and Iwo island. Seven species, namely, Periplaneta americana (Linnaeus, 1758), Periplaneta australasiae (Fabricius, 1775), Blattella lituricollis (Walker, 1868), Ony...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Infections and malignancies are common causes of pleural effusion. Among infectious causes, hyperinfection syndrome of Strongyloides stercoralis may occur in immunosuppressive patient. A 62-year-old man, known case of Non-Hodgkin lymphoma (NHL) was presented with recurrent NHL stage IV and had undergone salvage chemot...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:Escherichia coli (E. coli) strains are the major cause of urinary tract infections (UTI) and belong to the large group of extra-intestinal pathogenic E. coli. The purposes of this study were to determine the antibiotic resistance profile, virulence genes and phylogenetic background of E. coli isolates from UTI cases. ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:Angiostrongylus cantonensis, the nematode lungworm of rats, has a XX/X0 sex-determination mechanism. The chromosome constitution consists of 10 autosomes, with 2n=12, XX in the female and 2n=11, X0 in the male. Meiosis-I shows five bivalents and one univalent for the male worm, and six bivalents for the female worm. T...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:Cryptosporidiosis is a particular concern in immunocompromised individuals where symptoms may be severe. The aim of this study was to examine the epidemiological and molecular characteristics of Cryptosporidium infections in HIV/AIDS patients in Malaysia in order to identify risk factors and facilitate control measure...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-06-01 00:00:00
abstract：:Changes in the abundance of the house fly, Musca domestica, was studied for a period of one year in two poultry farms in Penang, Malaysia: one in Balik Pulau, located in Penang island, and the other in Juru, located on mainland Penang. The sampling of house flies were carried out from March 2007 to April 2008 using th...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:Hand, foot and mouth disease (HFMD) is a common viral infection among infants and children. The major causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16). Recently, coxsackievirus A6 (CVA6) infections were reported in neighboring countries. Infected infants and children may present with f...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts o...
journal_title：Tropical biomedicine
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:Entamoeba histolytica causes amoebic diarrhoea, colitis and liver abscess (ALA). Diagnosis of ALA is difficult, as most patients do not have simultaneous intestinal amoebic infection. At Hospital Universiti Sains Malaysia (HUSM), diagnosis of ALA relies on a combination of clinical findings, ultrasound examination of ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:This paper discusses the colonization of the stratiomyid species Ptecticus melanurus (Walker) (Diptera: Stratiomyidae) in monkey carrion and its potential for the determination of the minimum time since death (PMI). A study was conducted in a tropical forest at Bangi, Malaysia from 13 November 2009 to 8 June 201...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：:A focal outbreak of malaria at Sialimal sub-centre of Balasore district of Orissa was reported during the month of March, 2010. Three villages of the above block were affected. Regional Medical Research Centre, Bhubaneswar has conducted an entomological survey and a central clinic simultaneously, with door to do...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-06-01 00:00:00
abstract：:Studies were carried out on the residual efficacy of Bacillus thuringiensis H-14 (water dispersible granule, VectoBac ABG 6511) as direct application in the control of Aedes larvae in the field. Field Aedes sp populations in the earthen and glass jars were predetermined before initiation of the trial. On confirmation ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:This study explored the efficacy of Toxorhynchites splendens, predator of Aedes albopictus as a biocontrol agent. There was a negative correlation between Ae. albopictus larval population and Tx. splendens larval population in ovitraps (r=-0.287, R²=0.0821). The correlation is higher between the mean number of Ae. alb...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:A study was conducted to evaluate the infection status of Blastocystis hominis in children from four public schools in Phuttamonthon district, Nakhon Pathom province, Thailand during November to December 2004. A total of 814 faecal specimens were used for B. hominis cultivation using Jones' medium. Mixed infections wi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:The objective of this work is to evaluate the usefulness of a standardized clinical classification of hydroceles in lymphatic filariasis endemic countries to guide their surgical management. 64 patients with hydroceles were operated in 2009-2010, in Level II hospitals (WHO classification), during two visits to F...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:Poultry farming not only provides high nutritious food but also creates employment opportunity for rural masses. Documented evidences elaborates that helminth parasitism is most deciduous problem of chickens especially in developing world. Ascaridia (A.) galli, a nematode of small intestine, has been considered as the...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:Piophila casei (Linnaeus) (Diptera: Piophilidae) is reported from human cadavers in two separate forensic cases for the first time in Malaysia. Both bodies were found indoors. The first case, was that of a male of unknown nationality and age and also contained maggots of the muscid Ophyra spinigera (Stein). The second...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:In the present study, a total of 1,235 porcine serum samples were collected from 9 counties in Jilin Province (40°52'~46°18'N,121°38'~131°19'E), northeastern China from August to October 2013, and the seroprevalence of Toxoplasma gondii infection was tested by indirect haemagglutination assay (IHA). The results showed...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
abstract：:The disease melioidosis, caused by the soil bacteria Burkholderia pseudomallei, often manifests as acute septicemia with high fatality. Glycogen synthase kinase-3β (GSK3β) plays a key role during the inflammatory response induced by bacteria. We used a murine model of acute melioidosis to investigate the effects...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：:We report a patient presenting with chronic diarrhoea of unidentified etiology. Laboratory results showed microcytic anemia, peripheral eosinophilia with negative results of stool sample smears and stool concentration technique. Ancylostoma duodenale was found in the caecum and terminal ileum during colonoscopy. The p...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:A cohort study was performed to evaluate the incidence and risk factors of Giardia duodenalis infection in an orphanage in suburban area outside Bangkok, central Thailand. Stool specimens were examined for the presence of G. duodenalis in January 2007, May 2007 and January 2008. Of 892 stool specimens from 481 individ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-09-01 00:00:00
abstract：:This paper presents investigation of lungworm disease outbreaks that is based on retrospective examination of cases recorded between 1994 and 2000 on a government beef cattle breeding centre in the state of Pahang, peninsular Malaysia. The breed of cattle on the centre was Nelore and the mean population over a 7-year ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:Prior to the implementation of Haemophilus influenzae type b vaccination worldwide, H. influenzae has been one of the main causative agents of community acquired pneumonia and meningitis in children. Due to the lack of information on the characteristics of the H. influenzae isolates that have previously been col...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-09-01 00:00:00
abstract：:A total of 29 female black flies were captured by a hand net as they swarmed around humans in Tam Dao National Park, Vinh Phuc Province, Vietnam. They included one species of the subgenus Gomphostilbia (Simulium (Gomphostilbia) asakoae Takaoka & Davies) and five species of the subgenus Simulium, of which one species i...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:In the present study, serum samples from 402 sheep and 216 goats were collected from 5 counties in Jinzhou from August to October 2012 and antibodies to Toxoplasma gondii were detected by modified agglutination test (MAT). Overall, 104 (16.8%) had antibodies to T. gondii with antibody titres of 1:25 to 1:800. Seroposi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:This study was conducted to determine the prevalence of intestinal parasites among children and adult Orang Aslis (Aborigines) from different locations in Perak. Faecal samples were collected and analyzed using the direct smear and formal ether sedimentation technique. Some of the faecal samples were stained usi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-06-01 00:00:00
abstract：:Due to the disadvantages of human and equine rabies immunoglobulin, it is necessary to develop a substitute for HRIG and ERIG, especially for those people living in the developing countries. Because of higher affinity and lower immunogenicity of rabbit's immunoglobulins, anti-rabies immunoglobulins specific to rabies ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
