Abstract：
:Cryptosporidiosis is a particular concern in immunocompromised individuals where symptoms may be severe. The aim of this study was to examine the epidemiological and molecular characteristics of Cryptosporidium infections in HIV/AIDS patients in Malaysia in order to identify risk factors and facilitate control measures. A modified Ziehl-Neelsen acid fast staining method was used to test for the presence of Cryptosporidium oocysts in the stools of 346 HIV/AIDS patients in Malaysia. Standard coproscopical methods were used to identify infections with other protozoan or helminths parasites. To identify the species of Cryptosporidium, DNA was extracted and nested-PCR was used to amplify a portion of the SSU rRNA gene. A total of 43 (12.4%) HIV-infected patients were found to be infected with Cryptosporidium spp. Of the 43 Cryptosporidium-positive HIV patients, 10 (23.3%) also harboured other protozoa, and 15 (34.9%) had both protozoa and helminths. The highest rates of cryptosporidiosis were found in adult males of Malay background, intravenous drug users, and those with low CD4 T cell counts (i.e., < 200 cells/mm3). Most were asymptomatic and had concurrent opportunistic infections mainly with Mycobacterium tuberculosis. DNA sequence analysis of 32 Cryptosporidium isolates identified C. parvum (84.3%), C. hominis (6.3%), C. meleagridis (6.3%), and C. felis (3.1%). The results of the present study revealed a high prevalence of Cryptosporidium infection in hospitalized HIV/AIDS patients. The results also confirmed the potential significance of zoonotic transmission of C. parvum in HIV infected patients, as it was the predominant species found in this study. However, these patients were found to be susceptible to a wide range of Cryptosporidium species. Epidemiological and molecular characterization of Cryptosporidium isolates provides clinicians and researchers with further information regarding the origin of the infection, and may enhance treatment and control strategies.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Asma I,Sim BL,Brent RD,Johari S,Yvonne Lim ALsubject
Has Abstractpub_date
2015-06-01 00:00:00pages
310-22issue
2eissn
0127-5720issn
2521-9855journal_volume
32pub_type
杂志文章abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is known to cause nosocomial infections and is now becoming an emerging problem in veterinary medicine. The objective of the study was to determine the presence of MRSA in 100 cats and dogs sampled between November 2007 and April 2008 at the University Veterinary Hosp...
journal_title：Tropical biomedicine
pub_type： 杂志文章
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更新日期：2010-12-01 00:00:00
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journal_title：Tropical biomedicine
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journal_title：Tropical biomedicine
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更新日期：2011-12-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2004-12-01 00:00:00
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journal_title：Tropical biomedicine
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journal_title：Tropical biomedicine
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更新日期：2009-04-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2013-03-01 00:00:00
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更新日期：2015-03-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2008-04-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2012-12-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2015-09-01 00:00:00
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journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
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journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2008-04-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2010-08-01 00:00:00
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journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-06-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2008-08-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2012-09-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2013-06-01 00:00:00
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更新日期：2009-08-01 00:00:00
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更新日期：2014-12-01 00:00:00
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更新日期：2013-03-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2009-12-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2012-03-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2005-12-01 00:00:00
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journal_title：Tropical biomedicine
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更新日期：2014-03-01 00:00:00
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更新日期：2013-09-01 00:00:00
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更新日期：2015-06-01 00:00:00
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journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-03-01 00:00:00
