Abstract：
:Escherichia coli (E. coli) strains are the major cause of urinary tract infections (UTI) and belong to the large group of extra-intestinal pathogenic E. coli. The purposes of this study were to determine the antibiotic resistance profile, virulence genes and phylogenetic background of E. coli isolates from UTI cases. A total of 137 E. coli isolates were obtained from UTI samples. The antimicrobial susceptibility of confirmed isolates was determined by disk diffusion method against eight antibiotics. The isolates were examined to determine the presence and prevalence of selected virulence genes including iucD, sfa/focDE, papEF and hly. ECOR phylo-groups of isolates were determined by detection of yjaA and chuA genes and fragment TspE4.C2. The antibiogram results showed that 71% of the isolates were resistant to cefazolin, 60.42% to co-trimoxazole, 54.16% to nalidixic acid, 36.45% to gentamicin, 29.18% to ciprofloxacin, 14.58% to cefepime, 6.25% to nitrofurantoin and 0.00% to imipenem. Twenty-two antibiotic resistance patterns were observed among the isolates. Virulence genotyping of isolates revealed that 58.39% isolates had at least one of the four virulence genes. The iucD gene was the most prevalent gene (43.06%). The other genes including sfa/focDE, papEF and hly genes were detected in 35.76%, 18.97% and 2.18% isolates, respectively. Nine combination patterns of the virulence genes were detected in isolates. Phylotyping of 137 isolates revealed that the isolates fell into A (45.99%), B1 (13.14%), B2 (19.71%) and D (21.16%) groups. Phylotyping of multidrug resistant isolates indicated that these isolates are mostly in A (60.34%) and D (20.38%) groups. In conclusion, the isolates that possessed the iucD, sfa/focDE, papEF and hly virulence genes mostly belonged to A and B2 groups, whereas antibiotic resistant isolates were in groups A and D. Escherichia coli strains carrying virulence factors and antibiotic resistance are distributed in specific phylogenetic background.
journal_name
Trop Biomedjournal_title
Tropical biomedicineauthors
Adib N,Ghanbarpour R,Solatzadeh H,Alizade Hsubject
Has Abstractpub_date
2014-03-01 00:00:00pages
17-25issue
1eissn
0127-5720issn
2521-9855journal_volume
31pub_type
杂志文章abstract：:This study reports for the first time molecular detection of Anaplasma platys infection in 4 (13.3%) of 30 Malaysian dogs investigated. A low occurrence (3.3%) of Babesia gibsoni was also noted, being detected in one of the 30 dogs. Rickettsia, Bartonella, Orientia tsutsugamushi, and Ehrlichia DNA were not detected in...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-06-01 00:00:00
abstract：:Plaque assay procedure was carried out to determine the infectivity titration of dengue virus using Aedes albopictus C6/36 mosquito cell line. Cells were seeded in 6 wells plates and incubated until monolayer C6/36 cell lines were formed. Each well was exposed to a different dilution of dengue infected culture fluid, ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2005-12-01 00:00:00
abstract：:Hand, foot and mouth disease (HFMD) is a common viral infection among infants and children. The major causative agents of HFMD are enterovirus 71 (EV71) and coxsackievirus A16 (CVA16). Recently, coxsackievirus A6 (CVA6) infections were reported in neighboring countries. Infected infants and children may present with f...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:Analysis on fly artifacts produced by forensically important blowfly, Chrysomya megacephala (Fabricius) (Diptera:Calliphoridae), revealed several unique patterns. They can be divided into fecal spots, regurgitation spots and swiping stains. The characteristics of fecal spots are round with three distinct levels of pig...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-04-01 00:00:00
abstract：:In this case series report we aim to report a Disseminated Intravascular Coagulation (DIC)-like syndrome associated with Hemiscorpius lepturus sting in 4 individuals and a novel management protocol for this life-threatening condition that comprised partial exchange transfusion in severe scorpionism. ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-12-01 00:00:00
abstract：:A total of 105 blood samples from healthy horses from different stables in Yazd province, center of Iran, were examined for the presence of Theileria equi infection using parasitological and molecular methods. Out of the 105 samples, the parasitological method detected T. equi infection in 5 (4.76%) cases while the PC...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-03-01 00:00:00
abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is known to cause nosocomial infections and is now becoming an emerging problem in veterinary medicine. The objective of the study was to determine the presence of MRSA in 100 cats and dogs sampled between November 2007 and April 2008 at the University Veterinary Hosp...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:This is the first report of Synthesiomyia nudiseta (Wulp) (Diptera: Muscidae) on a human corpse discovered in a high-rise building in Malaysia. On 5 March 2008, a decomposing body of an adult female was found on the top floor of a thirteen-story building in Kuala Lumpur, Malaysia. Her body was colonized by S. nu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:This paper presents investigation of lungworm disease outbreaks that is based on retrospective examination of cases recorded between 1994 and 2000 on a government beef cattle breeding centre in the state of Pahang, peninsular Malaysia. The breed of cattle on the centre was Nelore and the mean population over a 7-year ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2007-06-01 00:00:00
abstract：:Routine surveillance on resistant status of field mosquito populations is important to implement suitable strategies in order to prevent pest outbreaks. WHO test kit bioassay is the most frequent bioassay used to investigate the susceptibility status of field-collected mosquitoes, as it is relatively convenient ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
abstract：:The objective of this work is to evaluate the usefulness of a standardized clinical classification of hydroceles in lymphatic filariasis endemic countries to guide their surgical management. 64 patients with hydroceles were operated in 2009-2010, in Level II hospitals (WHO classification), during two visits to F...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:Plasmodim vivax malaria accounts for more than 90% of malaria cases in Sri Lanka. There is limited information on the genetic heterogeneity of P. vivax in endemic areas of the country. Here we have assessed the potential of two P. vivax genes as genetic markers for their use in genotyping parasites collected from the ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:Angiostrongylus cantonensis, the nematode lungworm of rats, has a XX/X0 sex-determination mechanism. The chromosome constitution consists of 10 autosomes, with 2n=12, XX in the female and 2n=11, X0 in the male. Meiosis-I shows five bivalents and one univalent for the male worm, and six bivalents for the female worm. T...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2009-12-01 00:00:00
abstract：:In the present study, serum samples from 402 sheep and 216 goats were collected from 5 counties in Jinzhou from August to October 2012 and antibodies to Toxoplasma gondii were detected by modified agglutination test (MAT). Overall, 104 (16.8%) had antibodies to T. gondii with antibody titres of 1:25 to 1:800. Seroposi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2015-09-01 00:00:00
abstract：:The disease melioidosis, caused by the soil bacteria Burkholderia pseudomallei, often manifests as acute septicemia with high fatality. Glycogen synthase kinase-3β (GSK3β) plays a key role during the inflammatory response induced by bacteria. We used a murine model of acute melioidosis to investigate the effects...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-12-01 00:00:00
abstract：:A survey of cockroach fauna was carried out on the 3 inhabited islands of the Ogasawara chain island of Japan, namely, Chichijima island, Hahajima island and Iwo island. Seven species, namely, Periplaneta americana (Linnaeus, 1758), Periplaneta australasiae (Fabricius, 1775), Blattella lituricollis (Walker, 1868), Ony...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：:Signal fly, Scholastes sp. (Diptera: Platystomatidae) was observed associated with animal carcasses in Malaysia. The first observation was on a monkey carcass, which was killed by using a handgun and immediately placed in a forested area in Gombak, Selangor while the second observation was on a pig that died of natura...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:FT-IR spectra of liver tissue isolated from mice, Mus musculus, have been recorded in the region of 4000 - 400 cm-1 for normal, mercury treated and recovery phase. In this study, the total protein content was found to be decreased in the liver tissues after treatment with median-lethal dose of mercuric chloride. The m...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2005-06-01 00:00:00
abstract：:Scanning electron microscope (SEM) images of two dust mites, Sturnophagoides brasiliensis and Sturnophagoides halterophilus, are presented to provide an improved visualization of the taxonomic characters of these mites. Sturnophagoides halterophilus can be differentiated from S. brasiliensis by their expanded genu and...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2008-12-01 00:00:00
abstract：:The adulticidal activity of methanol extracts from three Malaysian plants namely Acorus calamus Linn., Litsea elliptica Blume and Piper aduncum Linn. against adult of Aedes aegypti (L.) were studied. Standard WHO bioassay tests were used to evaluate the effectiveness of these plant extracts. The hexane fraction from m...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2004-12-01 00:00:00
abstract：:This study was conducted to investigate the low prevalence of Dirofilaria immitis in dogs in Johor Bahru as reported by veterinary practitioners, using wet blood mount, Knott's Concentration Test and two heartworm antigen test kits (IDEXX Canine SNAP® 4Dx and RapiGEN®). This study also compared the two test kits...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:One hundred and sixty eight rats were trapped from the National Service Training Centres (NSTC) in Kelantan and Terengganu from October 2008 to May 2009. Microscopic agglutination test (MAT) was performed to detect the presence of agglutinating antibodies to Leptospira among the rats caught. All the MAT positive rats ...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:Limited data is available on the prevalence of parasitic infections among the island communities in Malaysia with most studies performed between 1960s-1980s. This study was conducted to determine the current prevalence status of parasitic infections among communities living in Pangkor Island Peninsular Malaysia. A tot...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:A total of 29 female black flies were captured by a hand net as they swarmed around humans in Tam Dao National Park, Vinh Phuc Province, Vietnam. They included one species of the subgenus Gomphostilbia (Simulium (Gomphostilbia) asakoae Takaoka & Davies) and five species of the subgenus Simulium, of which one species i...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2014-12-01 00:00:00
abstract：:Sugarcane bagasse is a renewable resource that can be used to produce biopesticide for the control of mosquito vectors. In the present study, we demonstrated that cane processed bagasse could be used to produce Bacillus thuringiensis serovar israelensis (Bti) for control of mosquito vectors viz: Culex quinquefasciatus...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2013-09-01 00:00:00
abstract：:A study was conducted to evaluate the infection status of Blastocystis hominis in children from four public schools in Phuttamonthon district, Nakhon Pathom province, Thailand during November to December 2004. A total of 814 faecal specimens were used for B. hominis cultivation using Jones' medium. Mixed infections wi...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-06-01 00:00:00
abstract：:Entamoeba histolytica, the causative agent of human amoebiasis remains a significant cause of morbidity and mortality in developing countries and is responsible for up to 100,000 deaths worldwide each year. Entamoeba dispar, morphologically indistinguishable from E. histolytica is more common in humans in many parts o...
journal_title：Tropical biomedicine
pub_type： 杂志文章,评审
doi：
更新日期：2011-08-01 00:00:00
abstract：:Using standard WHO methodology, this study investigated the susceptibility of 4(th) instar Aedes aegypti (L) and Culex annulirostris (Skuse) larvae to three extracts from Callitris glaucophylla (J. Thompson & L. Johnson) (1: steam distillation extract, 2: liquefied refrigerant gas extract, and 3: methanol reflux extra...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2006-12-01 00:00:00
abstract：:Understanding the genetic diversity, extent and distribution of variant forms of Plasmodium vivax parasites is crucial in the development of effective control measures and in Orissa, a hyperendemic state in the eastern part of India, the polymorphic nature of P. vivax isolates is largely lacking. The result of the stu...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:This study explored the efficacy of Toxorhynchites splendens, predator of Aedes albopictus as a biocontrol agent. There was a negative correlation between Ae. albopictus larval population and Tx. splendens larval population in ovitraps (r=-0.287, R²=0.0821). The correlation is higher between the mean number of Ae. alb...
journal_title：Tropical biomedicine
pub_type： 杂志文章
doi：
更新日期：2011-08-01 00:00:00
