Molecular analysis of the amphioxus frontal eye unravels the evolutionary origin of the retina and pigment cells of the vertebrate eye.

abstract：:Crosses between closely related animal species often result in male hybrids that are sterile, and the molecular and functional basis of genetic factors for hybrid male sterility is of great interest. Here, we report a molecular and functional analysis of HMS1, a region of 9.2 kb in chromosome 3 of Drosophila mauritian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liénard MA,Araripe LO,Hartl DL

更新日期：2016-07-19 00:00:00

abstract：:Adaptation of photosynthesis in marine environment has been examined in two strains of the green, picoeukaryote Ostreococcus: OTH95, a surface/high-light strain, and RCC809, a deep-sea/low-light strain. Differences between the two strains include changes in the light-harvesting capacity, which is lower in OTH95, and i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cardol P,Bailleul B,Rappaport F,Derelle E,Béal D,Breyton C,Bailey S,Wollman FA,Grossman A,Moreau H,Finazzi G

更新日期：2008-06-03 00:00:00

abstract：:The distribution of proenkephalin and [Met]enkephalin immunoreactivities in the bovine hypothalamo-neurohypophyseal system was studied by use of specific antisera. Proenkephalin and [Met]enkephalin immunoreactivities were found in magnocellular neuronal cell bodies in the dorsal part of the supraoptic nuclei and in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vanderhaeghen JJ,Lotstra F,Liston DR,Rossier J

更新日期：1983-08-01 00:00:00

abstract：:We describe here the successful establishment of a reverse genetics system for rotavirus (RV), a member of the Reoviridae family whose genome consists of 10-12 segmented dsRNA. The system is based on the recombinant vaccinia virus T7 RNA polymerase-driven procedure for supplying artificial viral mRNA in the cytoplasm....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Komoto S,Sasaki J,Taniguchi K

更新日期：2006-03-21 00:00:00

abstract：:The actin cytoskeleton is a critical regulator of cytoplasmic architecture and mechanics, essential in a myriad of physiological processes. Here we demonstrate a liquid phase of actin filaments in the presence of the physiological cross-linker, filamin. Filamin condenses short actin filaments into spindle-shaped dropl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weirich KL,Banerjee S,Dasbiswas K,Witten TA,Vaikuntanathan S,Gardel ML

更新日期：2017-02-28 00:00:00

abstract：:Apoptosis has been shown to be a significant form of cell loss in many diseases. Detachment of photoreceptors from the retinal pigment epithelium, as seen in various retinal disorders, causes photoreceptor loss and subsequent vision decline. Although caspase-dependent apoptotic pathways are activated after retinal det...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trichonas G,Murakami Y,Thanos A,Morizane Y,Kayama M,Debouck CM,Hisatomi T,Miller JW,Vavvas DG

更新日期：2010-12-14 00:00:00

abstract：:We explored the detection of genome-wide hypomethylation in plasma using shotgun massively parallel bisulfite sequencing as a marker for cancer. Tumor-associated copy number aberrations (CNAs) could also be observed from the bisulfite DNA sequencing data. Hypomethylation and CNAs were detected in the plasma DNA of pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan KC,Jiang P,Chan CW,Sun K,Wong J,Hui EP,Chan SL,Chan WC,Hui DS,Ng SS,Chan HL,Wong CS,Ma BB,Chan AT,Lai PB,Sun H,Chiu RW,Lo YM

更新日期：2013-11-19 00:00:00

abstract：:We present data that demonstrate that three MR elements isolated from wild populations of Drosophila melanogaster on two continents can cause large deletions of the X chromosome in males. The deleted chromosomes, termed mini-X chromosomes, are induced at a frequency of approximately 1:4000 in chromosomes that are init...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green MM,Yamamoto MT,Miklos GL

更新日期：1987-07-01 00:00:00

abstract：:During endoplasmic reticulum-associated degradation, the multifunctional AAA ATPase p97 is part of a protein degradation complex. p97 associates via its N-terminal domain with various cofactors to recruit ubiquitinated substrates. It also interacts with alternative substrate-processing cofactors, such as Ufd2, Ufd3, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao G,Zhou X,Wang L,Li G,Schindelin H,Lennarz WJ

更新日期：2007-05-22 00:00:00

abstract：:Gilles de la Tourette syndrome (TS) is characterized by tics, which are transiently worsened by stress, acute administration of dopaminergic drugs, and by subtle deficits in motor coordination and sensorimotor gating. It represents the most severe end of a spectrum of tic disorders that, in aggregate, affect ∼ 5% of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu M,Kobets A,Du JC,Lennington J,Li L,Banasr M,Duman RS,Vaccarino FM,DiLeone RJ,Pittenger C

更新日期：2015-01-20 00:00:00

abstract：:Eukaryotic translation initiation factor (eIF) 5 is crucial for the assembly of the eukaryotic preinitiation complex. This activity is mediated by the ability of its C-terminal HEAT domain to interact with eIF1, eIF2, and eIF3 in the multifactor complex and with eIF4G in the 48S complex. However, the binding sites for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamamoto Y,Singh CR,Marintchev A,Hall NS,Hannig EM,Wagner G,Asano K

更新日期：2005-11-08 00:00:00

abstract：:Classical feed-forward inhibition involves an excitation-inhibition sequence that enhances the temporal precision of neuronal responses by narrowing the window for synaptic integration. In the input layer of the cerebellum, feed-forward inhibition is thought to preserve the temporal fidelity of granule cell spikes dur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Duguid I,Branco T,Chadderton P,Arlt C,Powell K,Häusser M

更新日期：2015-10-20 00:00:00

abstract：:We expressed a cDNA encoding the Arabidopsis thaliana defense-related protein ELI3-2 in Escherichia coli to determine its biochemical function. Based on a protein database search, this protein was recently predicted to be a mannitol dehydrogenase [Williamson, J. D., Stoop, J. M. H., Massel, M. O., Conkling, M. A. & Ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Somssich IE,Wernert P,Kiedrowski S,Hahlbrock K

更新日期：1996-11-26 00:00:00

abstract：:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berlyn MB,Last RL,Fink GR

更新日期：1989-06-01 00:00:00

abstract：:Expression of alphavbeta3- or alphavbeta5-integrins and selectins is widespread on blood cells and endothelial cells. Here we report that human tumor cells injected s.c. into mice lacking beta3- or beta3/beta5-integrins or various selectins show enhanced tumor growth compared with growth in control mice. There was inc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taverna D,Moher H,Crowley D,Borsig L,Varki A,Hynes RO

更新日期：2004-01-20 00:00:00

abstract：:Although it has long been recognized that the enteric community of bacteria that inhabit the human distal intestinal track broadly impacts human health, the biochemical details that underlie these effects remain largely undefined. Here, we report a broad MS-based metabolomics study that demonstrates a surprisingly lar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wikoff WR,Anfora AT,Liu J,Schultz PG,Lesley SA,Peters EC,Siuzdak G

更新日期：2009-03-10 00:00:00

abstract：:The CRR1 (Copper Response Regulator) locus, required for both activating and repressing target genes of a copper- and hypoxia-sensing pathway in Chlamydomonas, encodes a 1,232-residue candidate transcription factor with a plant-specific DNA-binding domain named SBP, ankyrin repeats, and a C-terminal Cys-rich region, w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kropat J,Tottey S,Birkenbihl RP,Depège N,Huijser P,Merchant S

更新日期：2005-12-20 00:00:00

abstract：:Oxidative stress is a ubiquitous threat to all aerobic organisms and has been implicated in numerous pathological conditions such as cancer. Here we demonstrate a pivotal role for E2F1, a cell cycle regulatory transcription factor, in cell tolerance of oxidative stress. Cells lacking E2F1 are hypersensitive to oxidati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Graves JD,Lee YJ,Liu K,Li G,Lin FT,Lin WC

更新日期：2020-06-30 00:00:00

abstract：:Short oxygen-halogen interactions have been known in organic chemistry since the 1950s and recently have been exploited in the design of supramolecular assemblies. The present survey of protein and nucleic acid structures reveals similar halogen bonds as potentially stabilizing inter- and intramolecular interactions t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Auffinger P,Hays FA,Westhof E,Ho PS

更新日期：2004-11-30 00:00:00

abstract：:Translocations involving c-myc and an Ig locus have been reported rarely in human multiple myeloma (MM). Using specific fluorescence in situ hybridization probes, we show complex karyotypic abnormalities of the c-myc or L-myc locus in 19 of 20 MM cell lines and approximately 50% of advanced primary MM tumors. These ab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shou Y,Martelli ML,Gabrea A,Qi Y,Brents LA,Roschke A,Dewald G,Kirsch IR,Bergsagel PL,Kuehl WM

更新日期：2000-01-04 00:00:00

abstract：:To study the behavior of hematopoietic stem cells in vivo, we transplanted glucose-6-phosphate dehydrogenase (G6PD) heterozygous (female Safari) cats with small amounts of autologous marrow. The G6PD phenotypes of erythroid burst-forming units and granulocyte/macrophage colony-forming units were repeatedly assayed for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abkowitz JL,Persik MT,Shelton GH,Ott RL,Kiklevich JV,Catlin SN,Guttorp P

更新日期：1995-03-14 00:00:00

abstract：:A method to identify 5-methylcytosine (m5Cyt) in DNA immobilized on nitrocellulose paper by using antibody against m5Cyt raised in rabbits is described. Immobilized restriction fragments of DNA are incubated first with purified antibody against m5Cyt and then with goat anti-rabbit IgG labeled with 125I. Restriction fr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sano H,Royer HD,Sager R

更新日期：1980-06-01 00:00:00

abstract：:Communication about prescription drugs ought to be a paragon of public science communication. Unfortunately, it is not. Consumers see $4 billion of direct-to-consumer advertising annually, which typically fails to present data about how well drugs work. The professional label--the Food and Drug Administration's (FDA) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz LM,Woloshin S

更新日期：2013-08-20 00:00:00

abstract：:We describe in this study punchless, a nonpathogenic mutant from the rice blast fungus M. grisea, obtained by plasmid-mediated insertional mutagenesis. As do most fungal plant pathogens, M. grisea differentiates an infection structure specialized for host penetration called the appressorium. We show that punchless dif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Clergeot PH,Gourgues M,Cots J,Laurans F,Latorse MP,Pepin R,Tharreau D,Notteghem JL,Lebrun MH

更新日期：2001-06-05 00:00:00

abstract：:High-valent iron-oxo species are thought to be intermediates in the catalytic cycles of oxygenases and peroxidases. An attractive route to these iron-oxo intermediates involves laser flash-quench oxidation of ferric hemes, as demonstrated by our work on the ferryl (compound II) and ferryl porphyrin radical cation (com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ener ME,Lee YT,Winkler JR,Gray HB,Cheruzel L

更新日期：2010-11-02 00:00:00

abstract：:Chromosomal locations of members of the xenotropic-related env gene family in the mouse genome have been determined. Endonuclease restriction site polymorphisms detected by molecular hybridization were used to study the inheritance of mink cell-focus inducing and xenotropic env gene-related sequences in recombinant in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blatt C,Mileham K,Haas M,Nesbitt MN,Harper ME,Simon MI

更新日期：1983-10-01 00:00:00

abstract：:We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hyd...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada K,Kunishima N,Mayanagi K,Ohnishi T,Nishino T,Iwasaki H,Shinagawa H,Morikawa K

更新日期：2001-02-13 00:00:00

abstract：:Coastal hypoxia (dissolved oxygen ≤ 2 mg/L) is a growing problem worldwide that threatens marine ecosystem services, but little is known about economic effects on fisheries. Here, we provide evidence that hypoxia causes economic impacts on a major fishery. Ecological studies of hypoxia and marine fauna suggest multipl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith MD,Oglend A,Kirkpatrick AJ,Asche F,Bennear LS,Craig JK,Nance JM

更新日期：2017-02-14 00:00:00

abstract：:The tendency for genetic architectures to exhibit epistasis among mutations plays a central role in the modern synthesis of evolutionary biology and in theoretical descriptions of many evolutionary processes. Nevertheless, few studies unquestionably show whether, and how, mutations typically interact. Beneficial mutat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sanjuán R,Moya A,Elena SF

更新日期：2004-10-26 00:00:00

abstract：:CD2 (T11, sheep erythrocyte receptor) is a surface antigen of the human T-lymphocyte lineage. cDNA clones encoding CD2 have been isolated by using the purified, denatured CD2 to raise a rat antiserum. Positive clones were recognized in a phage lambda gt11 expression library prepared from the human leukemia T-cell line...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sewell WA,Brown MH,Dunne J,Owen MJ,Crumpton MJ

更新日期：1986-11-01 00:00:00