Abstract:
OBJECTIVES:We aimed to investigate the possible effects of vimentin (Vim) and citrullinated Vim (cVim) on proliferation capacity, pro-inflammatory cytokine secretion, and the expression of peptidylarginine deiminase type 4 (PADI4) and receptor activator of nuclear factor kappa B ligand (RANKL) in cultured fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA) and osteoarthritis (OA) patients. METHOD:Human native Vim was citrullinated with rabbit PAD in vitro and detected using a Western blot assay with anti-modified citrulline antibody (anti-MC Ab). FLSs from RA or OA synovial samples were stimulated with Vim or cVim. Cell proliferation capacity was determined using the Celltiter 96 AQueous cell proliferation assay. The concentrations of tumour necrosis factor (TNF)-α, interleukin (IL)-1, and IL-17 were measured by enzyme-linked immunosorbent assay (ELISA). The expression of PADI4 and RANKL was measured by real-time polymerase chain reaction (RT-PCR) and a Western blot assay. RESULTS:Our Western blot assay with anti-MC Ab indicated that the amount of cVim increased significantly after Vim had been incubated with rabbit PAD in vitro. The proliferation capacity and secretion of TNF-α and IL-1 were significantly enhanced in the FLSs of RA patients when treated with cVim. However, when treated with Vim, an inhibitory effect on the proliferation capacity was noted in the FLSs from RA and also from OA patients. cVim significantly increased the expression of PADI4 and RANKL in the FLSs from RA patients. CONCLUSION:cVim seems to have remarkable biological effects on RA as confirmed by the stimulation of proliferation capacity, pro-inflammatory cytokine secretion, and PADI4 and RANKL expression in the FLSs of RA patients.
journal_name
Scand J Rheumatoljournal_title
Scandinavian journal of rheumatologyauthors
Fan LY,He DY,Wang Q,Zong M,Zhang H,Yang L,Sun LSdoi
10.3109/03009742.2012.670263subject
Has Abstractpub_date
2012-10-01 00:00:00pages
354-8issue
5eissn
0300-9742issn
1502-7732journal_volume
41pub_type
杂志文章abstract:OBJECTIVE:To investigate the feasibility of magnetic resonance imaging (MRI)-guided high power focused ultrasound (FUS) to perform synovectomy noninvasively. METHODS:Five New Zealand white male rabbit knees with experimentally induced arthritis underwent MRI-guided thermal surgery by high power (60 W/10 s) sonication....
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009749950155607
更新日期:1999-01-01 00:00:00
abstract::The significance of C-reactive protein (CRP) elevation in patients with systemic lupus erythematosus (SLE) in the pretreatment stage was assessed with reference to other laboratory data obtained simultaneously. Sixteen of 31 cases were CRP-positive, and the CR-positive group contained significantly more cases with uri...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009748109095302
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVES:Foot orthoses are commonly prescribed as an intervention for people with rheumatoid arthritis (RA). Data relating to the cost-effectiveness of foot orthoses in people with RA are limited. The aim was to evaluate the clinical and cost-effectiveness of two types of foot orthoses in people with established RA. ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章,随机对照试验
doi:10.1080/03009742.2016.1196500
更新日期:2017-05-01 00:00:00
abstract::Arthritic affection of the upper cervical spine is relatively common in rheumatoid arthritis (RA). Atlanto-axial subluxations are also reported to occur frequently, but it seems that neurological symptoms due to the subluxations seldom occur. We have studied 104 consecutive in-patients. In a group of 50 non-arthritics...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009748409111302
更新日期:1984-01-01 00:00:00
abstract:OBJECTIVE:Aseptic loosening is one of the most important problems that can occur after total hip arthroplasty (THA). In this study, we analysed levels of large tenascin-C (TN-C) variants and compared them in pseudosynovial fluid from patients with aseptic loosening after THA with those in synovial fluid from patients u...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740510026733
更新日期:2005-11-01 00:00:00
abstract::The clinical outcome and tolerability following treatment with samarium-153 particulate hydroxyapatite was evaluated in patients with persistent rheumatoid knee synovitis. The clinical review of 18 patients treated with intra-articular samarium-153 particulate hydroxyapatite combined with triamcinolone hexacetonide wh...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009749609065647
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:To determine the effect of inflammation through exposure to tumour necrosis factor (TNF)alpha on T lymphocytes in patients with systemic lupus erythematosus (SLE). METHODS:We studied the effect of TNFalpha on T-lymphocyte apoptosis in patients with SLE, rheumatoid arthritis (RA), and in healthy controls. Apo...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740802409496
更新日期:2009-03-01 00:00:00
abstract::Objective: Vasculopathy in systemic sclerosis (SSc) is characterized by the obliteration of arterioles and a reduced capillary density in various tissues. In SSc, atrophic alterations of the choroid have been suggested based on morphological data acquired by optical coherence tomography (OCT). In this study, we aimed ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009742.2019.1641616
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVES:Schnitzler syndrome (SchS) is an autoinflammatory disorder characterized by chronic urticaria, fever, and monoclonal gammopathy. The success of interleukin-1 (IL-1) blocking therapies suggests a crucial role for IL-1 in disease induction. The aim of this study is to perform a comprehensive analysis of IL-1 f...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009742.2018.1550210
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVE:Infectious brain lesions (IBLs) are life-threatening in patients with systemic lupus erythematosus (SLE). The aim of this study was to determine the prevalence of IBL in SLE patients and the clinical characteristics of SLE patients with IBL. METHODS:Medical charts of 15 consecutive SLE patients with IBL admi...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009742.2012.680607
更新日期:2012-01-01 00:00:00
abstract::We investigated the frequency of gut inflammation and the role of gut lesion in the pathogenesis of the ankylosing spondylitis (AS) in Korean patients. Ileocolonoscopy and biopsy of the colon and terminal ileum were performed on 24 Korean patients with AS. Endoscopic lesions were observed in 7 patients (29.2%). The le...
journal_title:Scandinavian journal of rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/03009749709065722
更新日期:1997-01-01 00:00:00
abstract:OBJECTIVE:To determine whether HLA-DR alleles are associated with the development and clinical features of systemic sclerosis (SSc) in Koreans. METHODS:Seventy-nine patients (74 women and five men; 45 diffuse types and 34 limited types; mean age at diagnosis 43.9 years) fulfilling the American College of Rheumatology ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740510026751
更新日期:2006-01-01 00:00:00
abstract::We report the case of a 50-year-old man who presented with systemic vasculitis associated with Fasciola hepatica infection. The patient presented with severe skin, kidney, spleen, ophthalmic, and neurological compromise. An immunological examination for primary vasculitis was negative and other infections were discoun...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740500303231
更新日期:2006-03-01 00:00:00
abstract:OBJECTIVE:To establish by ultrasonography (US) the frequency of calcaneal entheses involvement in erosive osteoarthritis (EOA), nodal osteoarthritis (NOA), RA and PsA, and to compare these results in order to aid clinicians in the differential diagnosis among these diseases. A comparison between US results and radiogra...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740310003721
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVES:To compare prognosis parameters and arterial site involvement in Takayasu arteritis (TA) patients with disease onset at age ≤ 18 and ≥ 21 years. METHODS:Sixty-two TA patients [American College of Rheumatology (ACR) and European League Against Rheumatism/Paediatric Rheumatology European Society (EULAR/PreS) ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009741003742730
更新日期:2010-11-01 00:00:00
abstract::The aim of this study was to evaluate the presence of PAF-acether (PAF), its specific degrading enzyme acetylhydrolase, and tumor necrosis factor (TNF) concentrations in blood and synovial fluid (SF) from patients with active RA. The variations of the mediators were also evaluated after corticosteroid perfusions in 7 ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009749509099308
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVES:To determine the prevalence and predictors of influenza and influenza-like symptoms in patients with rheumatoid arthritis (RA). METHOD:Questionnaires were sent to patients registered as having RA and they were asked to fill in per month any period and details of influenza-like symptoms and vaccination. An e...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009742.2012.670729
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVES:To determine the serum concentration of tumour necrosis factor (TNF)-related weak inducer of apoptosis (TWEAK) in patients with rheumatoid arthritis (RA) and to investigate the relationship between TWEAK level and disease activity, proinflammatory cytokine levels, and response to anti-TNF treatment. METHODS...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009740801898608
更新日期:2008-05-01 00:00:00
abstract::The effect of transcutaneous nerve stimulation (TNS) on joint pain was studied in patients with rheumatoid arthritis. Three different forms of TNS were used in an attempt to evaluate the influence of placebo effects. The results were evaluated by means of loading tests and from the patients' own assessments of pain re...
journal_title:Scandinavian journal of rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1978-01-01 00:00:00
abstract::Using the fluorescence polarization of 1.6-diphenyl-1, 3, 5-hexatriene, the membrane fluidity of lymphocytes obtained from rheumatoid arthritis patients was measured during 12 months of auranofin treatment. Significant differences in fluorescence polarization values were observed following auranofin treatment. The cor...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009748909102104
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVES:Measurement of serum biomarkers at disease onset may improve prediction of disease course in patients with early rheumatoid arthritis (RA). We evaluated the multi-biomarker disease activity (MBDA) score and early changes in MBDA score for prediction of 28-joint Disease Activity Score based on C-reactive prot...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章,随机对照试验
doi:10.1080/03009742.2018.1464206
更新日期:2019-01-01 00:00:00
abstract:OBJECTIVES:We investigated the antibody levels against early antigens of Epstein-Barr virus (EBV), cytomegalovirus (CMV), and human herpesvirus 6 (HHV6) in systemic lupus erythematosus (SLE) patients and healthy controls, and further correlated these antibodies to haematology/biochemistry, serology, and disease activit...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009742.2014.973061
更新日期:2015-01-01 00:00:00
abstract::Rheumatoid synovial tissue cell proliferation was studied by incubating fresh synovial tissue samples with [3H]-thymidine in vitro. The samples were collected at operation from 7 rheumatoid patients. It was found that 0.9-4.9% of the total cell population, excluding the synovial lining cells, became labelled. ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009747709095429
更新日期:1977-01-01 00:00:00
abstract::Pain intensity, muscle strength, static muscle endurance, pressure pain threshold, and pain tolerance at tender points and control points were assessed in 31 patients with fibromyalgia (FM), before and after intravenous administration of morphine (9 patients), lidocaine (11 patients), and ketamine (11 patients). The t...
journal_title:Scandinavian journal of rheumatology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/03009749509095181
更新日期:1995-01-01 00:00:00
abstract:OBJECTIVES:To assess the prevalence of interstitial lung disease (ILD) in patients with different forms of connective tissue disease (CTD) using non-invasive procedures including high-resolution computed tomography (HRCT) and to evaluate the relationship between the imaging and functional status of the patients. METHO...
journal_title:Scandinavian journal of rheumatology
pub_type: 临床试验,杂志文章
doi:10.1080/03009740600844381
更新日期:2006-09-01 00:00:00
abstract::The correlation among the various markers of activated T cells (soluble interleukin 2 receptor (sIL-2R), HLA-DR+ and HLA-DP+ T cells, proliferating cell nuclear antigen (PCNA) positive lymphocytes) were examined in patients with systemic lupus erythematosus (SLE), and related to the cell cycle. The concentration of sI...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009749709065662
更新日期:1997-01-01 00:00:00
abstract::An account is given of a viscometer which can be used for measuring the viscosity of synovial fluid when only small quantities are avaliable (0.15 ml) and taking into account the non-Newtonian character of the fluid. A technical description of the apparatus is given. A series of experiments are reported in which bovin...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract:OBJECTIVES:Giant cell arteritis (GCA) is a systemic disease with extensive vascular involvement. The aim of this study was to investigate the cumulative incidence of large vessel involvement (LVI) in GCA, the distribution of vessels involved, and predictors for LVI. METHOD:Patients with biopsy-proven GCA in a defined ...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.1080/03009742.2016.1190984
更新日期:2017-05-01 00:00:00
abstract:OBJECTIVES:Reactive arthritis (ReA) is a sterile joint inflammation triggered by a remote infection and associated with human leucocyte antigen (HLA)-B27. Its pathogenesis is unknown, but abnormal response to microbial structures or endogenous inflammatory mediators may be involved. We studied responses in leucocyte si...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009742.2012.664649
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVES:The aims of this study were to measure the levels of interleukin (IL)-33 and ST2 and T-helper (Th)2-associated cytokines (IL-13, IL-4, IL-5) in patients with ankylosing spondylitis (AS), and examine the correlation of serum cytokine levels with disease activity and laboratory parameters. METHOD:Serum IL-33,...
journal_title:Scandinavian journal of rheumatology
pub_type: 杂志文章
doi:10.3109/03009742.2012.735700
更新日期:2013-01-01 00:00:00