Tenascin-C levels in pseudosynovial fluid of loose hip prostheses.

Abstract:

OBJECTIVE:Aseptic loosening is one of the most important problems that can occur after total hip arthroplasty (THA). In this study, we analysed levels of large tenascin-C (TN-C) variants and compared them in pseudosynovial fluid from patients with aseptic loosening after THA with those in synovial fluid from patients undergoing primary THA (control). METHODS:Pseudosynovial fluid samples (n = 24) were obtained by aspiration at the time of revision THA performed due to aseptic loosening. Synovial fluid samples (n = 12) were obtained by aspiration at the time of primary THA. Expression of TN-C splice variants was examined using immunoblotting. TN-C levels were measured using an enzyme-linked immunosorbent assay (ELISA) system that we developed previously. RESULTS:Western blotting showed the presence of large TN-C variants in pseudosynovial fluid of artificial joints with loosening. TN-C levels were approximately three times higher in pseudosynovial fluid of loose artificial joints (median 151.9 ng/mL) than in synovial fluid controls (median 50.1 ng/mL) (p = 0.035). CONCLUSION:Levels of TN-C including large variant subunits are elevated in pseudosynovial fluid of loose artificial joints, indicating that TN-C is a useful novel biochemical marker of loose hip prostheses.

journal_name

Scand J Rheumatol

authors

Hasegawa M,Sudo A,Nagakura T,Hirata H,Kinoshita N,Yoshida T,Uchida A

doi

10.1080/03009740510026733

keywords:

subject

Has Abstract

pub_date

2005-11-01 00:00:00

pages

464-8

issue

6

eissn

0300-9742

issn

1502-7732

pii

V45X2817856325X5

journal_volume

34

pub_type

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