Abstract：
:Lacking a blood supply and having a low cellular density, articular cartilage has a minimal ability for self-repair. Therefore, wide-ranging cartilage damage rarely resolves spontaneously. Cartilage damage is typically treated by chondrocyte transplantation, mosaicplasty or microfracture. Recent advances in tissue engineering have prompted research on techniques to repair articular cartilage damage using a variety of transplanted cells. We studied the repair and regeneration of cartilage damage using layered chondrocyte sheets prepared on a temperature-responsive culture dish. We previously reported achieving robust tissue repair when covering only the surface layer with layered chondrocyte sheets when researching partial-thickness defects in the articular cartilage of domestic rabbits. The present study was an experiment on the repair and regeneration of articular cartilage in a minipig model of full-thickness defects. Good safranin-O staining and integration with surrounding tissues was achieved in animals transplanted with layered chondrocyte sheets. However, tissue having poor safranin-O staining-not noted in the domestic rabbit experiments-was identified in some of the animals, and the subchondral bone was poorly repaired in these. Thus, although layered chondrocyte sheets facilitate articular cartilage repair, further investigations into appropriate animal models and culture and transplant conditions are required.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Ebihara G,Sato M,Yamato M,Mitani G,Kutsuna T,Nagai T,Ito S,Ukai T,Kobayashi M,Kokubo M,Okano T,Mochida Jdoi
10.1016/j.biomaterials.2012.01.056subject
Has Abstractpub_date
2012-05-01 00:00:00pages
3846-51issue
15eissn
0142-9612issn
1878-5905pii
S0142-9612(12)00156-1journal_volume
33pub_type
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