Abstract:
:We report the fabrication of a one-pot antigen system that delivers antigen to dendritic cells (DCs) and tracks their in vivo migration after injection. Multifunctional polymer nanoparticles containing ovalbumin protein, magnetic resonance imaging contrast agents (iron oxide nanoparticles), and near-infrared fluorophores (indocyanine green, ICG), MPN-OVA, were prepared using a double emulsion method. The MPN-OVA was efficiently taken up by the dendritic cells and subsequently localized in the lysosome. Flow cytometry analysis revealed an increase in the uptake of OVA antigen by MPN-OVA at 37 °C, when compared with soluble OVA protein. We found that MPN-OVA had no effect on DC surface expression of MHC class I, costimulatory (CD80, CD86) or adhesion (CD54) molecules or the ability of DCs to mature in response to LPS. Following the uptake of MPN-OVA, exogenous OVA antigen was delivered to the cytoplasm, and OVA peptides were presented on MHC class I molecules, which enhanced OVA antigen-specific cross-presentation to OT-1 T cells and CD8OVA1.3 T cell hybridoma in vitro. The immunization of mice with MPN-OVA-treated DCs induced OVA-specific CTL activity in draining lymph nodes. The presence of MPN allowed us to monitor the migration of DCs via lymphatic drainage using NIR fluorescence imaging, and the homing of DCs into the lymph nodes was imaged using MRI. This system has potential for use as a delivery system to induce T cell priming and to image DC-based immunotherapies.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Noh YW,Jang YS,Ahn KJ,Lim YT,Chung BHdoi
10.1016/j.biomaterials.2011.05.013subject
Has Abstractpub_date
2011-09-01 00:00:00pages
6254-63issue
26eissn
0142-9612issn
1878-5905pii
S0142-9612(11)00519-9journal_volume
32pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::Endochondral ossification plays a key role in the bone healing process, which requires normal cartilage callus formation. Progranulin (PGRN) growth factor is known to enhance chondrocyte differentiation and endochondral ossification during development, yet whether PGRN also plays a role in bone regeneration remains un...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.05.030
更新日期:2013-09-01 00:00:00
abstract::In this work, analytical transmission electron microscopy (TEM) was used to study the nanostructure of mineralised ivory dentine, in order to gain a clearer understanding of the relationship between the organic (collagen fibrils) and inorganic (calcium phosphate apatite crystals) components. Thin sections prepared by ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.03.025
更新日期:2010-07-01 00:00:00
abstract::Theranostic nanoagents which integrate diagnostic and therapeutic moieties into a single platform have attracted broad attention in cancer therapy, however the development of more effective and less toxic diagnostic and therapeutic interventions is still of great urgency. Herein, novel core-shell PB@MIL-100(Fe) dual m...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.05.027
更新日期:2016-09-01 00:00:00
abstract::The development of an efficient colorectal cancer therapy is currently a public health priority. In the present work, we proposed a multifunctional theranostic micellar drug delivery system utilizing cationic PDMA-block-poly(ε-caprolactone) (PDMA-b-PCL) micelles as nanocarriers of SN-38 (7-ethyl-10-hydroxycamptothecin...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.01.068
更新日期:2016-04-01 00:00:00
abstract::Chitosans with various degrees of deacetylation were synthesized by acetylation with acetic anhydride. These chitosans were evaluated for efficacy of nanoparticle formation, DNA binding efficiency, morphology, and in vitro and in vivo gene transfection efficiency. DNA binding efficacy was reduced as degree of deacetyl...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.12.036
更新日期:2004-10-01 00:00:00
abstract::Prosthetic heart valves epitomize both the triumphant advance of cardiac surgery in its early days and its stagnation into a retrospective, exclusive first world discipline of late. Fifty-two years after the first diseased heart valve was replaced in a patient, prostheses largely represent the concepts of the 1960s wi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2007.09.033
更新日期:2008-02-01 00:00:00
abstract::The cytocompatibility of amphiphilic, thermoresponsive and chemically crosslinkable macromers was examined in vitro. Macromers synthesized from pentaerythritol diacrylate monostearate, N-isopropylacrylamide, acrylamide and hydroxyethyl acrylate in different molar ratios and with varying molecular weights and lower cri...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2009.05.029
更新日期:2009-09-01 00:00:00
abstract::The in vivo properties of a new kind of calcium phosphate cement were investigated in this study. Calcium phosphate cement was implanted as paste into femoral bone and dorsal muscle of dogs for 3 and 6 months, and as prehardened form into thigh muscles of dogs for 1, 2 and 6 months. Histology was performed on thin un-...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00016-8
更新日期:2000-06-01 00:00:00
abstract::Driven by the clinical need for a strong tissue adhesive with elastomeric material properties, a departure from legacy crosslinking chemistries was sought as a multipurpose platform for tissue mending. A fresh approach to bonding wet substrates has yielded a synthetic biomaterial that overcomes the drawbacks of free-r...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120215
更新日期:2020-11-01 00:00:00
abstract::Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer ste...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119705
更新日期:2020-03-01 00:00:00
abstract::Osteoinductive biomaterials are promising for bone repair. There is no direct proof that bone marrow mesenchymal stem cells (BMSCs) home to non-osseous sites and participate in ectopic bone formation induced by osteoinductive bioceramics. The objective of this study was to use a sex-mismatched beagle dog model to inve...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.12.010
更新日期:2013-03-01 00:00:00
abstract::The poor biocompatibility of metallic coronary stents which leads to un-satisfying restenosis rates is mainly caused by contact activation of blood cells, smooth muscle cells and endothelial cells. Mimicking a metal surface with a biocompatible coating that actively suppresses mechanisms leading to restenosis may over...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00244-1
更新日期:2001-04-01 00:00:00
abstract::Atherosclerosis is the leading cause of many fatal cardiovascular and cerebrovascular diseases. Whereas nanomedicines are promising for targeted therapy of atherosclerosis, great challenges remain in development of effective, safe, and translational nanotherapies for its treatment. Herein we hypothesize that non-proin...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.07.035
更新日期:2017-10-01 00:00:00
abstract::Cell labeling using magnetic nanoparticles is an increasingly used approach in noninvasive behavior tracking, in vitro separation of cancer stem cells (CSCs), and CSC-based research in cancer therapy. However, the impact of magnetic labeling on the biological properties of targeted CSCs, such as self-renewal, prolifer...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.01.058
更新日期:2012-05-01 00:00:00
abstract::Chitosan-silica xerogel hybrid membranes were fabricated using a sol-gel process and their potential applications in guided bone regeneration (GBR) were investigated in terms of their in vitro cellular activity and in vivo bone regeneration ability. TEM observation revealed that the silica xerogel was dispersed in the...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2008.10.025
更新日期:2009-02-01 00:00:00
abstract::The in vivo evaluation, in New Zealand rabbits, of a SiO(2)-P(2)O(5)-CaO sol-gel glass and a SiO(2)-P(2)O(5)-CaO-MgO glass-ceramic, both bioactive in Kokubo's simulated body fluid (SBF), is presented. Bone defects, performed in the lateral aspect of distal right femoral epiphysis, 5mm in diameter and 4mm in depth, wer...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.12.009
更新日期:2004-08-01 00:00:00
abstract::Biomimetic strategies focusing on presenting short bioadhesive oligopeptides, including the arginine-glycine-aspartic acid (RGD) motif present in numerous adhesive proteins, on a non-fouling support have emerged as promising approaches to improve cellular activities and healing responses. Nevertheless, these bio-inspi...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2006.06.027
更新日期:2006-11-01 00:00:00
abstract::The objective of this study was to investigate the moisture absorption, physical-mechanical and bioadhesive properties of hot-melt extruded hydroxypropylcellulose (HPC) films containing polymer additives. These additives included polyethylene glycol (PEG) 5%, polycarbophil 5%, carbomer 5%, Eudragit E-100 5%, and sodiu...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(00)00046-6
更新日期:2000-07-01 00:00:00
abstract::We report a new template-directed method for the fabrication of hydroxyapatite (HAp) sponges by using amino-acid-coated HAp nanoparticles dispersed within a viscous polysaccharide (dextran sulfate) matrix, and describe the use of these materials for the viability and proliferation of human bone marrow stromal cells. T...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2005.04.037
更新日期:2005-11-01 00:00:00
abstract::This article reports a novel preparation method of poly(ethylene glycol) (PEG)-polystyrene (PST) amphiphilic block copolymers with well-defined block lengths by using photopolymerization of an iniferter, benzyl N,N-diethyldithiocarbamate. PEG macroiniferters, which were prepared by end-capping of PEG monomethyl ethers...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(98)00252-x
更新日期:1999-05-01 00:00:00
abstract::In order to develop a more effective self-etching primer, with a longer lasting shelf life, we designed a self-etching primer comprised of methacrylamide, N-methacryloyl glycine, NMGly. In this study, the hydrolytic stability of the amide portion in the NMGly was examined. The difference in the hydrolytic stability be...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00616-1
更新日期:2004-03-01 00:00:00
abstract::This study aimed to investigate certain processes of fluoride production which enable glass ionomer cements to leach fluoride. Two fluoroaluminosilicate glasses, G338 and LG26 were used. The free and total fluoride which could be dissolved from the glasses was measured, before and after acetic acid washing. Both glass...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00352-0
更新日期:2002-05-01 00:00:00
abstract::The clinical application of calcium phosphate cements (CPCs) composed of tetracalcium phosphate and dicalcium phosphate anhydrous has been limited because of its longer setting time, so that we developed the CPC in which the setting time was shortened to approximately 10 min. Aiming at clinical application, we evaluat...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00422-8
更新日期:2003-12-01 00:00:00
abstract::Islet transplantation is a method for the treatment of type 1 diabetes mellitus (DM) and has been widely performed around the world. The long-term cryopreservation of islets shows many advantages in the field of islet transplantation. Previous studies have described the development of sheet-type polyvinyl alcohol (PVA...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.01.088
更新日期:2010-05-01 00:00:00
abstract::A series of in vitro screening assays for the preliminary selection of biomaterials for use in the fabrication of artificial corneas (keratoprostheses) (KPros) have been investigated. These screening assays assessed the initial binding of inflammatory and cell adhesive proteins, activation of inflammatory proteins, ad...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00370-3
更新日期:2003-11-01 00:00:00
abstract::Brain tumors and brain metastases are among the deadliest malignancies of all human cancers, largely due to the cellular blood-brain and blood-tumor barriers that limit the delivery of imaging and therapeutic agents from the systemic circulation to tumors. Thus, improved strategies for brain tumor visualization and ta...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.07.028
更新日期:2015-10-01 00:00:00
abstract::Osteoclasts resorb bone at surfaces, leaving behind pits and trails where both mineral and organic phases of bone have been dissolved. Rough surface structures are deliberately imparted to synthetic implants, in order to improve osseointegration. The aim of this study is to characterize osteoclastic resorption pits on...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.06.009
更新日期:2010-10-01 00:00:00
abstract::Combination therapy is usually considered as a promising strategy owing to its advantages such as reduced doses, minimized side effects and improved therapeutic efficiency in a variety of diseases including diabetes. Here we synthesized a new highly intracellular stimuli-sensitive chitosan-graft-metformin (CS-MET) pro...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.09.013
更新日期:2015-12-01 00:00:00
abstract::It is known that the organic molecules can provide an effective means to manipulate the surface properties of the biodegradable ceramic. There are two ways to modify the surface of the biodegradable ceramic by organic molecules. The first one is through surface adsorption but organic molecules will easily be washed ou...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(01)00070-9
更新日期:2001-12-01 00:00:00
abstract::Modeling the interaction between the supportive stroma and the hematopoietic stem and progenitor cells (HSPC) is of high interest in the regeneration of the bone marrow niche in blood disorders. In this work, we present an injectable co-culture system to study this interaction in a coherent in vitro culture and in viv...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119665
更新日期:2020-02-01 00:00:00