Early tumor detection afforded by in vivo imaging of near-infrared II fluorescence.


:Cell-intrinsic reporters such as luciferase (LUC) and red fluorescent protein (RFP) have been commonly utilized in preclinical studies to image tumor growth and to monitor therapeutic responses. While extrinsic reporters that emit near infrared I (NIR-I: 650-950 nm) or near-infrared II (NIR-II: 1000-1700 nm) optical signals have enabled minimization of tissue autofluorescence and light scattering, it has remained unclear as to whether their use has afforded more accurate tumor imaging in small animals. Here, we developed a novel optical imaging construct comprised of rare earth lanthanide nanoparticles coated with biodegradable diblock copolymers and doped with organic fluorophores, generating NIR-I and NIR-II emissive bands upon optical excitation. Simultaneous injection of multiple spectrally-unique nanoparticles into mice bearing tumor implants established via intraperitoneal dissemination of LUC+/RFP+ OVCAR-8 ovarian cancer cells enabled direct comparisons of imaging with extrinsic vs. intrinsic reporters, NIR-II vs. NIR-I signals, as well as targeted vs. untargeted exogenous contrast agents in the same animal and over time. We discovered that in vivo optical imaging at NIR-II wavelengths facilitates more accurate detection of smaller and earlier tumor deposits, offering enhanced sensitivity, improved spatial contrast, and increased depths of tissue penetration as compared to imaging with visible or NIR-I fluorescent agents. Our work further highlights the hitherto underappreciated enhancements in tumor accumulation that may be achieved with intraperitoneal as opposed to intravenous administration of nanoparticles. Lastly, we found discrepancies in the fidelity of tumor uptake that could be obtained by utilizing small molecules for in vivo as opposed to in vitro targeting of nanoparticles to disseminated tumors.






Tao Z,Dang X,Huang X,Muzumdar MD,Xu ES,Bardhan NM,Song H,Qi R,Yu Y,Li T,Wei W,Wyckoff J,Birrer MJ,Belcher AM,Ghoroghchian PP




Has Abstract


2017-07-01 00:00:00












  • Potent killing of HBV-related hepatocellular carcinoma by a chimeric protein of anti-HBsAg single-chain antibody and truncated Bid.

    abstract::Targeted therapy is needed for hepatitis B virus (HBV)-mediated hepatocellular carcinoma (HCC) which shows overexpression of HBV surface antigen (HBsAg). We previously developed scFv15, a human single-chain antibody against HBsAg. Here we tested the strategic feasibility of scFv15-mediated delivery of apoptotic effect...


    pub_type: 杂志文章


    authors: Yan B,Ouyang Q,Zhao Z,Cao F,Wang T,Jia X,Meng Y,Jiang S,Liu J,Chen R,Jia L,Zhang R,Wen W,Jin B,Chen S,Zhao J,Yang A

    更新日期:2013-07-01 00:00:00

  • Effect of resin hydrophilicity and temperature on water sorption of dental adhesive resins.

    abstract::This study examined the effects of copolymer hydrophilicity and temperature on water sorption and solubility characteristics of five copolymer blends of increasing degree of hydrophilicity using gravimetric measurements. Six resin disks (15 mm in diameter x 1 mm in thickness) were prepared from each copolymer blend an...


    pub_type: 杂志文章


    authors: Yiu CK,King NM,Carrilho MR,Sauro S,Rueggeberg FA,Prati C,Carvalho RM,Pashley DH,Tay FR

    更新日期:2006-03-01 00:00:00

  • Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains for the simultaneous detection of multiple antigens.

    abstract::Simultaneous detection of multiple antigens by conventional immunological methods has been limited by the source of primary antibodies. Each antibody should be derived from a different host species (or subclass of immunoglobulin (Ig)) for suppressing the cross-reactions of secondary antibodies. Here we describe an inn...


    pub_type: 杂志文章


    authors: Iijima M,Matsuzaki T,Yoshimoto N,Niimi T,Tanizawa K,Kuroda S

    更新日期:2011-12-01 00:00:00

  • Intra-articular delivery of sinomenium encapsulated by chitosan microspheres and photo-crosslinked GelMA hydrogel ameliorates osteoarthritis by effectively regulating autophagy.

    abstract::Reduced expression of autophagy regulators has been observed in pathological cartilage in humans and mice. The present study aimed to investigate the synergistic therapeutic effect of promotion of chondrocyte autophagy via exposure to sinomenium (SIN) encapsulated by chitosan microspheres (CM-SIN) and photo-crosslinke...


    pub_type: 杂志文章


    authors: Chen P,Xia C,Mei S,Wang J,Shan Z,Lin X,Fan S

    更新日期:2016-03-01 00:00:00

  • Establishing the protein MIA (melanoma inhibitory activity) as a marker for chondrocyte differentiation.

    abstract::Melanoma inhibitory activity (MIA), also referred to as cartilage derived retinoic acid-sensitive protein (CD-RAP), is detected physiologically in cartilage tissue and pathologically in malignant melanomas. To measure MIA/CD-RAP quantitatively we developed a sensitive ELISA system. Recently, we described diagnostic ap...


    pub_type: 杂志文章


    authors: Bosserhoff AK,Buettner R

    更新日期:2003-08-01 00:00:00

  • An injectable conductive hydrogel encapsulating plasmid DNA-eNOs and ADSCs for treating myocardial infarction.

    abstract::Myocardial infarction (MI) leads to the mass death of cardiomyocytes accompanying with the unfavorable alternation of microenvironment, a fibrosis scar deprived of electrical communications, and the lack of blood supply in the infarcted myocardium. The three factors are inextricably intertwined and thus result in a co...


    pub_type: 杂志文章


    authors: Wang W,Tan B,Chen J,Bao R,Zhang X,Liang S,Shang Y,Liang W,Cui Y,Fan G,Jia H,Liu W

    更新日期:2018-04-01 00:00:00

  • Thermosensitive hydrogels based on polypeptides for localized and sustained delivery of anticancer drugs.

    abstract::Thermosensitive hydrogels based on poly(γ-ethyl-L-glutamate)-poly(ethylene glycol)-poly(γ-ethyl-L-glutamate) triblock copolymers (PELG-PEG-PELG) were prepared for localized and sustained delivery of anticancer drugs. The polypeptide-based hydrogels showed much lower critical gelation concentration than the traditional...


    pub_type: 杂志文章


    authors: Cheng Y,He C,Ding J,Xiao C,Zhuang X,Chen X

    更新日期:2013-12-01 00:00:00

  • Biodegradable poly(polyol sebacate) polymers.

    abstract::We have developed a family of synthetic biodegradable polymers that are composed of structural units endogenous to the human metabolism, designated poly(polyol sebacate) (PPS) polymers. Material properties of PPS polymers can be tuned by altering the polyol monomer and reacting stiochiometric ratio of sebacic acid. Th...


    pub_type: 杂志文章


    authors: Bruggeman JP,de Bruin BJ,Bettinger CJ,Langer R

    更新日期:2008-12-01 00:00:00

  • Extraction of high quality RNA from polysaccharide matrices using cetyltrimethylammonium bromide.

    abstract::Polysaccharides are increasingly being used as biomaterials for tissue engineering and regenerative medicine. Quantitative analysis of gene expression from cells in three-dimensional (3D) scaffolds requires extraction of messenger RNA, which is complicated in polysaccharide materials by ionic complexing between nuclei...


    pub_type: 杂志文章


    authors: Wang L,Stegemann JP

    更新日期:2010-03-01 00:00:00

  • Modification of layered atelocollagen: enzymatic degradation and cytotoxicity evaluation.

    abstract::Two kinds of layered atelocollagen materials cross-linked with hexamethylene diisocyanate (HMDIC), starch dialdehyde and glyoxal were enzymatically treated by bacterial collagenase. Evaluating collagenase digestion assay for these material showed progressive differences, particularly in the group of samples cross-link...


    pub_type: 杂志文章


    authors: Vizárová K,Bakos D,Rehákova M,Petríkova M,Panáková E,Koller J

    更新日期:1995-11-01 00:00:00

  • 5-FU loaded pHEMA drainage implants for glaucoma-filtering surgery: device design and in vitro release kinetics.

    abstract::Implantable monolithic and reservoir-like water-swellable drainage devices were developed for the subconjunctival sustained release of 5-fluorouracil (5-FU) in glaucoma-filtering surgery. A water-swellable matrix was formed of a copolymer of 2-hydroxyethyl methacrylate (HEMA) with different amounts of ethylene glycol ...


    pub_type: 杂志文章


    authors: Gökce M,Akata RF,Kiremitçi-Gümüşderelioğlu M

    更新日期:1996-05-01 00:00:00

  • Bottom-up assembly of salivary gland microtissues for assessing myoepithelial cell function.

    abstract::Myoepithelial cells are flat, stellate cells present in exocrine tissues including the salivary glands. While myoepithelial cells have been studied extensively in mammary and lacrimal gland tissues, less is known of the function of myoepithelial cells derived from human salivary glands. Several groups have isolated tu...


    pub_type: 杂志文章


    authors: Ozdemir T,Srinivasan PP,Zakheim DR,Harrington DA,Witt RL,Farach-Carson MC,Jia X,Pradhan-Bhatt S

    更新日期:2017-10-01 00:00:00

  • Nanopatterned muscle cell patches for enhanced myogenesis and dystrophin expression in a mouse model of muscular dystrophy.

    abstract::Skeletal muscle is a highly organized tissue in which the extracellular matrix (ECM) is composed of highly-aligned cables of collagen with nanoscale feature sizes, and provides structural and functional support to muscle fibers. As such, the transplantation of disorganized tissues or the direct injection of cells into...


    pub_type: 杂志文章


    authors: Yang HS,Ieronimakis N,Tsui JH,Kim HN,Suh KY,Reyes M,Kim DH

    更新日期:2014-02-01 00:00:00

  • Synergistic effects of Akt1 shRNA and paclitaxel-incorporated conjugated linoleic acid-coupled poloxamer thermosensitive hydrogel on breast cancer.

    abstract::The phosphoinositide 3-kinase/Akt1 signaling pathway has emerged as a target for cancer therapy. In this study, we aimed to develop a strategy to enhance Akt-targeted cancer therapy. We hypothesized that combination of Akt1-targeted therapy with conventional chemotherapy using paclitaxel-incorporated conjugated linole...


    pub_type: 杂志文章


    authors: Guo DD,Hong SH,Jiang HL,Kim JH,Minai-Tehrani A,Kim JE,Shin JY,Jiang T,Kim YK,Choi YJ,Cho CS,Cho MH

    更新日期:2012-03-01 00:00:00

  • Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells.

    abstract::The major problem in skin grafting is that tissue-engineered skin grafts after their transplantation are initially entirely dependent on diffusion. Since this process is slow and inefficient, nutrients, growth factors, and oxygen will insufficiently be supplied and the regenerating graft will undergo a physiological c...


    pub_type: 杂志文章


    authors: Klar AS,Güven S,Biedermann T,Luginbühl J,Böttcher-Haberzeth S,Meuli-Simmen C,Meuli M,Martin I,Scherberich A,Reichmann E

    更新日期:2014-06-01 00:00:00

  • A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics.

    abstract::With respect to the effect of material factors on calcium phosphate biomaterial-induced osteogenesis, the osteoinductive property of two kinds of porous hydroxyapatite ceramics, which were made by different producers, was investigated in dorsal muscles of dogs. One hydroxyapatite ceramic (S-HA), macroporous implants w...


    pub_type: 杂志文章


    authors: Yuan H,Kurashina K,de Bruijn JD,Li Y,de Groot K,Zhang X

    更新日期:1999-10-01 00:00:00

  • Receptor-targeted liposome-peptide nanocomplexes for siRNA delivery.

    abstract::RNA interference induced by double-stranded, small interfering RNA (siRNA) molecules has attracted great attention as a genetic therapeutic approach. Despite major advances in this field, new nanoparticle formulations are required for in vivo delivery of siRNA, particularly for tissue-specific delivery of siRNA reagen...


    pub_type: 杂志文章


    authors: Tagalakis AD,He L,Saraiva L,Gustafsson KT,Hart SL

    更新日期:2011-09-01 00:00:00

  • The optimization of porous polymeric scaffolds for chondrocyte/atelocollagen based tissue-engineered cartilage.

    abstract::To broaden the clinical application of cartilage regenerative medicine, we should develop an implant-type tissue-engineered cartilage with firmness and 3-D structure. For that, we attempted to use a porous biodegradable polymer scaffold in the combination with atelocollagen hydrogel, and optimized the structure and co...


    pub_type: 杂志文章


    authors: Tanaka Y,Yamaoka H,Nishizawa S,Nagata S,Ogasawara T,Asawa Y,Fujihara Y,Takato T,Hoshi K

    更新日期:2010-06-01 00:00:00

  • Adhesive bond strength and compliance for denture soft lining materials.

    abstract::Peel bond strength and tensile bond strength between three polyvinylsiloxane denture soft liners and a heat-cured acrylic resin denture base were measured using two adhesive systems. The soft lining materials differed only in regard of their filler content and compliance. The values of bond strength and mode of failur...


    pub_type: 杂志文章


    authors: McCabe JF,Carrick TE,Kamohara H

    更新日期:2002-03-01 00:00:00

  • Stability and cellular responses to fluorapatite-collagen composites.

    abstract::Fluorapatite (FA)-collagen composites were synthesized via a biomimetic coprecipitation method in order to improve the structural stability and cellular responses. Different amounts of ammonium fluoride (NH4F), acting as a fluorine source for FA, were added to the precipitation of the composites. The precipitated comp...


    pub_type: 杂志文章


    authors: Yoon BH,Kim HW,Lee SH,Bae CJ,Koh YH,Kong YM,Kim HE

    更新日期:2005-06-01 00:00:00

  • Time-dependent degradation of titanium osteoconductivity: an implication of biological aging of implant materials.

    abstract::The shelf life of implantable materials has rarely been addressed. We determined whether osteoconductivity of titanium is stable over time. Rat bone marrow-derived osteoblasts were cultured on new titanium disks (immediately after acid-etching), 3-day-old (stored after acid-etching for 3 days in dark ambient condition...


    pub_type: 杂志文章


    authors: Att W,Hori N,Takeuchi M,Ouyang J,Yang Y,Anpo M,Ogawa T

    更新日期:2009-10-01 00:00:00

  • Compaction assay: a rapid and simple in vitro method to assess the responsiveness of a biopolymer matrix to enzymatic modification.

    abstract::A rapid and simple in vitro method is described which measures the extent of unrecoiled solids compression when a complex biopolymer is subjected to a centrifugal force. This method, termed the compaction assay, was used to assess the response of purulent cystic fibrosis (CF) sputum samples to the addition of recombin...


    pub_type: 杂志文章


    authors: Daugherty AL,Patapoff TW,Clark RC,Sinicropi DV,Mrsny RJ

    更新日期:1995-05-01 00:00:00

  • Microwave pumped high-efficient thermoacoustic tumor therapy with single wall carbon nanotubes.

    abstract::The ultra-short pulse microwave could excite to the strong thermoacoustic (TA) shock wave and deeply penetrate in the biological tissues. Based on this, we developed a novel deep-seated tumor therapy modality with mitochondria-targeting single wall carbon nanotubes (SWNTs) as microwave absorbing agents, which act effi...


    pub_type: 杂志文章


    authors: Wen L,Ding W,Yang S,Xing D

    更新日期:2016-01-01 00:00:00

  • Blood-brain barrier dysfunction induced by silica NPs in vitro and in vivo: Involvement of oxidative stress and Rho-kinase/JNK signaling pathways.

    abstract::Silica nanoparticles (SiO2-NPs) has been extensively exploited in biomedical fields and mostly designed to enter the circulatory system, however, few studies focused on the potential adverse effects of SiO2-NPs exposure on the blood-brain barrier (BBB) that serves as a critical barrier between the central nervous syst...


    pub_type: 杂志文章


    authors: Liu X,Sui B,Sun J

    更新日期:2017-03-01 00:00:00

  • Synthesis and properties of novel block copolymers containing poly(lactic-glycolic acid) and poly(ethyleneglycol) segments.

    abstract::A synthetic process for obtaining high-molecular-weight block copolymers containing poly(lactic-glycolic acid) and poly(ethylene glycol) segments has been established. This process involves the reaction of poly(ethylene glycols) with phosgene, followed by polycondensation of the resulting alpha, omega-bis(chloroformat...


    pub_type: 杂志文章


    authors: Ferruti P,Penco M,D'Addato P,Ranucci E,Deghenghi R

    更新日期:1995-12-01 00:00:00

  • Polymeric microspheres as stabilizing anchors for oligonucleotide delivery to dendritic cells.

    abstract::The aim of this study is to evaluate a novel microspheric vector for delivery of oligonucleotides (ODN) into dendritic cells (DC). A requirement of decoy-based modulation of transcriptional activities in DC is that the ODN would have to accumulate inside the cell. Using an ex vivo DC culture model, we demonstrate that...


    pub_type: 杂志文章


    authors: Kovacs JR,Zheng Y,Shen H,Meng WS

    更新日期:2005-11-01 00:00:00

  • Biocatalytic synthesis of highly ordered degradable dextran-based hydrogels.

    abstract::We have prepared unique macroporous and ordered dextran-based hydrogels using a single-step biocatalytic transesterification reaction between dextran and divinyladipate in neat dimethylsulfoxide. These hydrogels show a unimodal distribution of interconnected pores with average diameters from 0.4 to 2.0 microm dependin...


    pub_type: 杂志文章


    authors: Ferreira L,Gil MH,Cabrita AM,Dordick JS

    更新日期:2005-08-01 00:00:00

  • Bioinspired membrane provides periosteum-mimetic microenvironment for accelerating vascularized bone regeneration.

    abstract::Periosteum plays a pivotal role in vascularization, ossification and remodeling during the healing process of bone injury. However, there are few studies focused on the construction of artificial implants with periosteum-mimetic effect. To emulate the primary role of natural periosteum or endosteal tissues in bone reg...


    pub_type: 杂志文章


    authors: Yang G,Liu H,Cui Y,Li J,Zhou X,Wang N,Wu F,Li Y,Liu Y,Jiang X,Zhang S

    更新日期:2021-01-01 00:00:00

  • Enhanced immunostimulatory effects of DNA-encapsulated peptide hydrogels.

    abstract::DNA that encodes tumor-specific antigens represents potential immunostimulatory agents. However, rapid enzymatic degradation and fragmentation of DNA during administration can result in limited vector expression and, consequently, poor efficacy. These challenges have necessitated the use of novel strategies for DNA de...


    pub_type: 杂志文章


    authors: Medina SH,Li S,Howard OM,Dunlap M,Trivett A,Schneider JP,Oppenheim JJ

    更新日期:2015-01-01 00:00:00

  • Improved tissue ingrowth and anchorage of expanded polytetrafluoroethylene by perforation: an experimental study in the rat.

    abstract::Although an expanded polytetrafluoroethylene patch is a suitable synthetic material to repair large abdominal wall defects, lack of tissue ingrowth into the patch may give rise to herniation at the fascia-patch interface. This study was undertaken to determine whether perforations and/or ethanol pretreatment improved ...


    pub_type: 杂志文章


    authors: Simmermacher RK,van der Lei B,Schakenraad JM,Bleichrodt RP

    更新日期:1991-01-01 00:00:00