Abstract：
:There is great demand for constructing well-organized three-dimensional (3D) tissues in vitro. Here, we developed a 3D stacked culture method using biodegradable poly(d,l-lactide-co- glycolide) (PLGA) membranes with defined topography. Pore size and porosity of the membranes can be controlled by changing the moisture content during fabrication. The optimized membrane served as a scaffold to manipulate small hepatocyte (SH) layers when they were stacked, while it degraded after stacking, resulting in the reorganization of the cells into a 3D stacked structure. Immunofluorescent staining for domain markers of cell polarity and electron microscopy confirmed that the cells in the 3D stacked structures recovered polarity. Furthermore, the cells exhibited improved liver-specific function as compared with cells in a monolayer. This 3D stacked culture may enable reconstruction of multilayered hepatic tissues with highly differentiated functions in vitro.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Kasuya J,Sudo R,Tamogami R,Masuda G,Mitaka T,Ikeda M,Tanishita Kdoi
10.1016/j.biomaterials.2011.12.039subject
Has Abstractpub_date
2012-03-01 00:00:00pages
2693-700issue
9eissn
0142-9612issn
1878-5905pii
S0142-9612(11)01509-2journal_volume
33pub_type
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