Reconstruction of cytosolic fumaric acid biosynthetic pathways in Saccharomyces cerevisiae.

abstract：BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...

journal_title：Microbial cell factories

authors： Kumar R,Shimizu K

更新日期：2010-01-27 00:00:00

abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

journal_title：Microbial cell factories

authors： Villaverde A

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However, hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins, but can...

journal_title：Microbial cell factories

authors： Pekarsky A,Veiter L,Rajamanickam V,Herwig C,Grünwald-Gruber C,Altmann F,Spadiut O

更新日期：2018-11-24 00:00:00

abstract：BACKGROUND:Bacteriocin-producing Lactic acid bacteria (LAB) have vast applications in human and animal health, as well as in food industry. The structural, immunity, regulatory, export and modification genes are required for effective bacteriocin biosynthesis. Variations in gene sequence, composition and organisation w...

journal_title：Microbial cell factories

authors： Tai HF,Foo HL,Abdul Rahim R,Loh TC,Abdullah MP,Yoshinobu K

更新日期：2015-06-16 00:00:00

abstract：BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...

journal_title：Microbial cell factories

authors： Santala S,Efimova E,Kivinen V,Larjo A,Aho T,Karp M,Santala V

更新日期：2011-05-18 00:00:00

abstract：BACKGROUND:Bifidobacterium spp. are representative probiotics that play an important role in the health of their hosts. Among various Bifidobacterium spp., B. bifidum BGN4 exhibits relatively high cell adhesion to colonic cells and has been reported to have various in vivo and in vitro bio functionalities (e.g., anti-a...

journal_title：Microbial cell factories

authors： Hong N,Ku S,Yuk K,Johnston TV,Ji GE,Park MS

更新日期：2021-01-19 00:00:00

abstract：BACKGROUND:Ganoderic acids (GAs), derived from the medicinal mushroom Ganoderma lucidum, possess anticancer and other important pharmacological activities. To improve production of GAs, a homologous farnesyl diphosphate synthase (FPS) gene was overexpressed in G. lucidum. Moreover, the influence of FPS gene overexpress...

journal_title：Microbial cell factories

authors： Fei Y,Li N,Zhang DH,Xu JW

更新日期：2019-06-28 00:00:00

abstract：BACKGROUND:Emergence of antibiotic resistance and growing consumer trend towards foods containing biopreservatives stimulated the search for alternative antimicrobials. This research is aimed at characterizing, investigating the mechanism of action, scale up optimization and evaluating the biopreservative potential of ...

journal_title：Microbial cell factories

authors： Wayah SB,Philip K

更新日期：2018-08-13 00:00:00

abstract：BACKGROUND:The sesquiterpenoid abscisic acid (ABA) is mostly known for regulating developmental processes and abiotic stress responses in higher plants. Recent studies show that ABA also exhibits a variety of pharmacological activities. Affordable and sustainable production will be required to utilize the compound in a...

journal_title：Microbial cell factories

authors： Otto M,Teixeira PG,Vizcaino MI,David F,Siewers V

更新日期：2019-11-25 00:00:00

abstract：BACKGROUND:Enantiopure 2-hydroxy acids are key intermediates for the synthesis of pharmaceuticals and fine chemicals. We present an enantioselective cascade biocatalysis using recombinant microbial cells for deracemization of racemic 2-hydroxy acids that allows for efficient production of enantiopure 2-hydroxy acids. ...

journal_title：Microbial cell factories

authors： Xue YP,Zeng H,Jin XL,Liu ZQ,Zheng YG

更新日期：2016-09-22 00:00:00

abstract：BACKGROUND:Targeting of cellular proteins to the extracellular environment is directed by a secretory signal sequence located at the N-terminus of a secretory protein. These signal sequences usually contain an N-terminal basic amino acid followed by a stretch containing hydrophobic residues, although no consensus signa...

journal_title：Microbial cell factories

authors： Yarimizu T,Nakamura M,Hoshida H,Akada R

更新日期：2015-02-14 00:00:00

abstract：:Amylase plays an important role in biotechnology industries, and Gram-positive bacterium Bacillus subtilis is a major host to produce heterogeneous α-amylases. However, the secretion stress limits the high yield of α-amylase in B. subtilis although huge efforts have been made to address this secretion bottleneck. In t...

journal_title：Microbial cell factories

authors： Yan S,Wu G

更新日期：2017-07-19 00:00:00

abstract：BACKGROUND:Cost-effective production of the highly effective anti-cancer drug, paclitaxel (Taxol®), remains limited despite growing global demands. Low yields of the critical taxadiene precursor remains a key bottleneck in microbial production. In this study, the key challenge of poor taxadiene synthase (TASY) solubili...

journal_title：Microbial cell factories

authors： Nowrouzi B,Li RA,Walls LE,d'Espaux L,Malcı K,Liang L,Jonguitud-Borrego N,Lerma-Escalera AI,Morones-Ramirez JR,Keasling JD,Rios-Solis L

更新日期：2020-11-02 00:00:00

abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:Corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications deriv...

journal_title：Microbial cell factories

authors： Sun H,Zhao D,Xiong B,Zhang C,Bi C

更新日期：2016-08-24 00:00:00

abstract：BACKGROUND:Activation of silent biosynthetic gene clusters (BGCs) in marine-derived actinomycete strains is a feasible strategy to discover bioactive natural products. Actinoalloteichus sp. AHMU CJ021, isolated from the seashore, was shown to contain an intact but silent caerulomycin A (CRM A) BGC-cam in its genome. Th...

journal_title：Microbial cell factories

authors： Xie Y,Chen J,Wang B,Chen T,Chen J,Zhang Y,Liu X,Chen Q

更新日期：2020-08-06 00:00:00

abstract：BACKGROUND:Epimutations arising from transcriptional slippage seem to have more important role in regulating gene expression than earlier though. Since the level and the fidelity of transcription primarily determine the overall efficiency of gene expression, all factors contributing to their decrease should be identifi...

journal_title：Microbial cell factories

authors： Wons E,Koscielniak D,Szadkowska M,Sektas M

更新日期：2018-09-21 00:00:00

abstract：BACKGROUND:The industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has ...

journal_title：Microbial cell factories

authors： Hossain AH,Li A,Brickwedde A,Wilms L,Caspers M,Overkamp K,Punt PJ

更新日期：2016-07-28 00:00:00

abstract：BACKGROUND:Gene therapy has gained an increasing interest in its anti-tumor efficiency. However, numerous efforts are required to promote them to clinics. In this study, a novel and efficient delivery platform based on bacterial magnetosomes (BMs) were developed, and the efficiency of BMs in delivering small interferin...

journal_title：Microbial cell factories

authors： Dai Q,Long R,Wang S,Kankala RK,Wang J,Jiang W,Liu Y

更新日期：2017-11-28 00:00:00

abstract：BACKGROUND:Oil palm empty fruit bunch (EFB) is a lignocellulosic waste produced in palm oil industry. EFB mainly consists of cellulose, hemicellulose (mainly xylan) and lignin and has a great potential to be reused. Converting EFB to fermentable sugars and value-added chemicals is a much better choice than treating EFB...

journal_title：Microbial cell factories

authors： Li Q,Ng WT,Wu JC

更新日期：2014-11-11 00:00:00

abstract：BACKGROUND:Intracellular metabolism of glucocorticoid hormones plays an important role in the pathogenesis of metabolic syndrome and regulates, among many physiological processes, collagen metabolism in skin. At the peripheral level the concentration of active glucocorticoids is mainly regulated by the 11β-hydroxystero...

journal_title：Microbial cell factories

authors： Vanella R,Callari R,Weston A,Heider H,Schwab MS,Kübler E

更新日期：2016-03-15 00:00:00

abstract：BACKGROUND:Due to their high energy density and compatible physical properties, several monoterpenes have been investigated as potential renewable transportation fuels, either as blendstocks with petroleum or as drop-in replacements for use in vehicles (both heavy and light-weight) or in aviation. Sustainable microbial...

journal_title：Microbial cell factories

authors： Zhuang X,Kilian O,Monroe E,Ito M,Tran-Gymfi MB,Liu F,Davis RW,Mirsiaghi M,Sundstrom E,Pray T,Skerker JM,George A,Gladden JM

更新日期：2019-03-18 00:00:00

abstract：BACKGROUND:cis, cis-Muconic acid is an important chemical that can be biosynthesized from simple substrates in engineered microorganisms. Recently, it has been shown that engineering microbial cocultures is an emerging and promising approach for biochemical production. In this study, we aim to explore the potential of ...

journal_title：Microbial cell factories

authors： Zhang H,Li Z,Pereira B,Stephanopoulos G

更新日期：2015-09-15 00:00:00

abstract：BACKGROUND:Calreticulin (CRT) resides in the endoplasmic reticulum (ER) and functions to chaperone proteins, ensuring proper folding, and intracellular Ca(2+) homeostasis. Emerging evidence shows that CRT is a multifunctional protein with significant roles in physiological and pathological processes with presence both ...

journal_title：Microbial cell factories

authors： Čiplys E,Žitkus E,Gold LI,Daubriac J,Pavlides SC,Højrup P,Houen G,Wang WA,Michalak M,Slibinskas R

更新日期：2015-10-15 00:00:00

abstract：:Controlling harmful algae blooms (HABs) using microbial algicides is cheap, efficient and environmental-friendly. However, obtaining high yield of algicidal microbes to meet the need of field test is still a big challenge since qualitative and quantitative analysis of algicidal compounds is difficult. In this study, w...

journal_title：Microbial cell factories

authors： Cai G,Zheng W,Yang X,Zhang B,Zheng T

更新日期：2014-05-24 00:00:00

abstract：BACKGROUND:Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Therefore, ample efforts are underway to rationally design therapies targeting the HCV non-structural prot...

journal_title：Microbial cell factories

authors： Anwar MI,Iqbal M,Yousef MS,Rahman M

更新日期：2013-11-16 00:00:00

abstract：BACKGROUND:Crocin is a carotenoid-derived natural product found in the stigma of Crocus spp., which has great potential in medicine, food and cosmetics. In recent years, microbial production of crocin has drawn increasing attention, but there were no reports of successful implementation. Escherichia coli has been engin...

journal_title：Microbial cell factories

authors： Wang W,He P,Zhao D,Ye L,Dai L,Zhang X,Sun Y,Zheng J,Bi C

更新日期：2019-07-05 00:00:00

abstract：BACKGROUND:The proteome reflects the available cellular machinery to deal with nutrients and environmental challenges. The most common E. coli strain BL21 growing in different, commonly employed media was evaluated using a detailed quantitative proteome analysis. RESULTS:The presence of preformed biomass precursor mol...

journal_title：Microbial cell factories

authors： Li Z,Nimtz M,Rinas U

更新日期：2014-03-23 00:00:00

abstract：BACKGROUND:Oxygen-evolving photoautotrophic organisms, like cyanobacteria, protect their photosynthetic machinery by a number of regulatory mechanisms, including alternative electron transfer pathways. Despite the importance in modulating the electron flux distribution between the photosystems, alternative electron tra...

journal_title：Microbial cell factories

authors： Thiel K,Patrikainen P,Nagy C,Fitzpatrick D,Pope N,Aro EM,Kallio P

更新日期：2019-11-05 00:00:00

abstract：BACKGROUND:The oleaginous yeast Yarrowia lipolytica is an organism of choice for the tailored production of various compounds such as biofuels or biopolymers. When properly engineered, it is capable of producing medium-chain-length polyhydroxyalkanoate (mcl-PHA), a biobased and biodegradable polymer that can be used as...

journal_title：Microbial cell factories

authors： Rigouin C,Lajus S,Ocando C,Borsenberger V,Nicaud JM,Marty A,Avérous L,Bordes F

更新日期：2019-05-31 00:00:00