Abstract:
:Angiotensin II (Ang II) has been demonstrated as a pro-inflammatory effect in acute lung injury, but studies of the effect of Ang II on the formation of pulmonary edema and alveolar filling remains unclear. Therefore, in this study the regulation of alveolar fluid clearance (AFC) and the expression of epithelial sodium channel (ENaC) by exogenous Ang II was verified. SD rats were anesthetized and were given Ang II with increasing doses (1, 10 and 100 μg/kg per min) via osmotic minipumps, whereas control rats received only saline vehicle. AT1 receptor antagonist ZD7155 (10 mg/kg) and inhibitor of cAMP degeneration rolipram (1 mg/kg) were injected intraperitoneally 30 min before administration of Ang II. The lungs were isolated for measurement of alveolar fluid clearance. The mRNA and protein expression of ENaC were detected by RT-PCR and Western blot. Exposure to higher doses of Ang II reduced AFC in a dose-dependent manner and resulted in a non-coordinate regulation of α-ENaC vs. the regulation of β- and γ-ENaC, however Ang II type 1 (AT1) receptor antagonist ZD7155 prevented the Ang II-induced inhibition of fluid clearance and dysregulation of ENaC expression. In addition, exposure to inhibitor of cAMP degradation rolipram blunted the Ang II-induced inhibition of fluid clearance. These results indicate that through activation of AT(1) receptor, exogenous Ang II promotes pulmonary edema and alveolar filling by inhibition of alveolar fluid clearance via downregulation of cAMP level and dysregulation of ENaC expression.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Deng J,Wang DX,Deng W,Li CY,Tong J,Ma Hdoi
10.1016/j.resp.2011.11.009subject
Has Abstractpub_date
2012-04-15 00:00:00pages
53-61issue
1eissn
1569-9048issn
1878-1519pii
S1569-9048(11)00398-3journal_volume
181pub_type
杂志文章abstract:OBJECTIVE:The aim of this study was to investigate whether autophagy is enhanced in alveolar epithelial cells as well as its role in alveolar barrier function of in lipopolysaccharide (LPS)-induced ALI mice. MATERIALS AND METHODS:Autophagy inhibitors, including 3-methyladenine (3-MA) and chloroquine (CLQ), and LPS wer...
journal_title:Respiratory physiology & neurobiology
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更新日期:2021-01-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
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journal_title:Respiratory physiology & neurobiology
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2007.01.004
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journal_title:Respiratory physiology & neurobiology
pub_type: 临床试验,杂志文章
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journal_title:Respiratory physiology & neurobiology
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journal_title:Respiratory physiology & neurobiology
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doi:10.1016/j.resp.2015.03.007
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2007.10.010
更新日期:2008-02-29 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,多中心研究,随机对照试验
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更新日期:2018-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-03-15 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(03)00199-x
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2014.07.009
更新日期:2014-12-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2014.04.004
更新日期:2014-08-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2018.07.011
更新日期:2019-01-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2020.103615
更新日期:2021-01-06 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.resp.2010.11.004
更新日期:2011-01-31 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2012.02.010
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
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pub_type: 临床试验,杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2011.06.006
更新日期:2011-09-15 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2007.02.012
更新日期:2007-12-15 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.resp.2018.05.003
更新日期:2018-09-01 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2008.02.007
更新日期:2008-05-31 00:00:00
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journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2004.04.012
更新日期:2004-07-20 00:00:00
abstract::In order to examine, whether the lobeline-induced cough is a true reflex or a voluntary effort to get rid of its irritating sensations in the upper respiratory tract, we systematically studied the cough response to lobeline, of subjects who were unable to make conscious discriminations i.e. were either comatose (n=4) ...
journal_title:Respiratory physiology & neurobiology
pub_type: 临床试验,杂志文章
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更新日期:2005-01-15 00:00:00
abstract::Intermittent hypoxia induces plasticity in neural networks controlling breathing and cardiovascular function. Studies demonstrate that mechanisms causing cardiorespiratory plasticity rely on intracellular signalling pathways that are activated by specific neurotransmitters. Peptides such as serotonin, PACAP and orexin...
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pub_type: 杂志文章,评审
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更新日期:2016-06-01 00:00:00
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