Abstract:
:Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs against human DROSHA and DICER1 or miRNA mimics or inhibitors. Wounding assays were performed and the cells were observed using time-lapse microscopy. The area of damage was calculated at chosen time points, followed by data analysis. Cells with silenced global miRNA expression showed a significantly slower repair rate compared to the control cells (p=0.001). For miR-328, we observed significantly delayed repair in cells transfected with the inhibitor compared to control (p=0.02). Global microRNA silencing significantly decreased the repair rate of airway epithelial cells in vitro, indicating an important role of miRNA in the regulation of wound repair and that miR-328, possibly involved in actin pathway, may be a potent modifier of this process.
journal_name
Respir Physiol Neurobioljournal_title
Respiratory physiology & neurobiologyauthors
Narożna B,Langwinski W,Jackson C,Lackie P,Holloway JW,Szczepankiewicz Adoi
10.1016/j.resp.2017.03.010subject
Has Abstractpub_date
2017-08-01 00:00:00pages
59-65eissn
1569-9048issn
1878-1519pii
S1569-9048(17)30095-2journal_volume
242pub_type
杂志文章abstract::We developed and tested a semi-automated algorithm to generate large data sets of ventilatory information (amplitude, premotor drive and timing) across a range of motor behaviors. Adult spontaneously breathing, anesthetized mice (n = 27) underwent measurements of transdiaphragmatic pressure (Pdi) during eupnea, hypoxi...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2015.05.009
更新日期:2015-08-15 00:00:00
abstract::Seizure-induced cardiorespiratory autonomic dysfunction is a major cause of sudden unexpected death in epilepsy (SUDEP), and the underlying mechanism is unclear. Seizures lead to increased synthesis, and release of glutamate, pituitary adenylate cyclase activating polypeptide (PACAP), and other neurotransmitters, and ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.01.003
更新日期:2016-06-01 00:00:00
abstract::Pulmonary mechanics [static (Est) and dynamic (Edyn) elastances, resistive (DeltaP1) and viscoelastic pressures (DeltaP2)], histology, and bronchoalveolar lavage fluid (BALF) from BALB/c mice were analysed 1, 24, 48 and 72 h after intravenous injection of saline or Bothrops jararaca crude venom [0.3 (V0.3) or 1 (V1) m...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2003.10.002
更新日期:2004-01-15 00:00:00
abstract::In response to the increase in oxygen tension at birth, the resistance pulmonary arteries dilate, while the ductus arteriosus constricts. Although modulated by the endothelium, these opposite responses are intrinsic to the vascular smooth muscle. While still controversial, it seems likely that during normoxia the prod...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/s1569-9048(02)00054-x
更新日期:2002-08-22 00:00:00
abstract::Newborn mammals exhibit a biphasic hypoxic ventilatory response (HVR) in which an initial increase in ventilation is followed by a decline back toward baseline levels. The magnitude of the secondary decline diminishes with postnatal age, but this transition occurs earlier in rat pups reared in moderate hyperoxia. This...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2020.103483
更新日期:2020-09-01 00:00:00
abstract::High frequency spinal cord stimulation (HF-SCS) is a method of inspiratory muscle activation resulting in phrenic motoneuron activation via stimulation of spinal cord pathways. The specific pathways mediating this response, however, are unknown. The aim of this study was to assess the potential role of upper cervical ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2012.12.003
更新日期:2013-03-01 00:00:00
abstract::We tested the hypotheses that: (1) carotid body size can be measured by computed tomographic angiography (CTA) with high inter-observer agreement, and (2) patients with sleep apnea exhibit larger carotid bodies than those without sleep apnea. A chart review was conducted from patients who underwent neck CTA and polyso...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.10.013
更新日期:2017-02-01 00:00:00
abstract::During prolonged anoxia at low temperature, freshwater turtles develop high plasma concentrations of both lactate and calcium. At these concentrations the formation of the complex, calcium lactate, normally of little biological significance because of the low association constant for the reaction, significantly reduce...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2004.06.020
更新日期:2004-12-15 00:00:00
abstract::Intermittent hypoxia induces plasticity in neural networks controlling breathing and cardiovascular function. Studies demonstrate that mechanisms causing cardiorespiratory plasticity rely on intracellular signalling pathways that are activated by specific neurotransmitters. Peptides such as serotonin, PACAP and orexin...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2016.03.012
更新日期:2016-06-01 00:00:00
abstract::The cardiovascular system undergoes major changes in stress with space flight primarily related to the elimination of the head-to-foot gravitational force. A major observation has been that the central venous pressure is not elevated early in space flight yet stroke volume is increased at least early in flight. Recent...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2009.07.017
更新日期:2009-10-01 00:00:00
abstract::Changes in airway temperature can result in respiratory responses such as cough, bronchoconstriction and mucosal secretion after cold exposure and hyperventilation after heat exposure. In the present investigation, we examined the activity of bronchopulmonary receptors in response to activators of thermo-sensitive tra...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2011.05.011
更新日期:2011-08-15 00:00:00
abstract::In aquatic vertebrates, peripheral O(2) chemoreceptors initiate compensatory physiological and behavioural responses to hypoxia, beginning at very early stages of development, to maintain sufficient gas exchange across the skin or gills. This review highlights the morphological and physiological studies, particularly ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2006.01.004
更新日期:2006-11-01 00:00:00
abstract::Slices of rat carotid bodies, or cultured glomus cells, were used to study intercellular coupling. This phenomenon occurs because gap junctions allow passage of currents and dyes from one cell to another. There is a two-way resistive coupling between glomus cells (GC/GC coupling), which is accompanied by activity of i...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2007.01.013
更新日期:2007-07-01 00:00:00
abstract::Autonomic reflex responses are critical in restoring changes to circulatory factors reduced beyond the domain of homeostasis. Intermittent hypoxia triggers repeated activation of chemoreflexes, resulting in baroreflex dysfunction and widespread changes in cellular and neuronal activity regulated by sensory/motor pathw...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2018.08.008
更新日期:2019-07-01 00:00:00
abstract::Swallowing is a powerful inhibitor of respiratory rhythm in infants. The present study was aimed at investigating the influence of states of alertness on non-nutritive swallowing (NNS) frequency, on NNS and respiration coordination, and on bursts of NNS frequency in newborn lambs. Six full term newborn lambs were inst...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(02)00220-3
更新日期:2003-03-28 00:00:00
abstract::Expression of the transcription factor FOXP2 is linked to brain circuits that control motor function and speech. Investigation of FOXP2 protein expression in respiratory areas of the ponto-medullary brainstem of adult rat revealed distinct rostro-caudal expression gradients. A high density of FOXP2 immunoreactive nucl...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2018.01.014
更新日期:2018-04-01 00:00:00
abstract::Previously we have reported that association of cigarette smoke (CS) and chronic hypoxia (CH) interact positively to physiopathologically remodel pulmonary circulation. In present study we have exposed guinea pigs to CS smoke (four cigarettes/day; 3 months; CS) and to chronic hypoxia (12% O(2), 15 days; CH) alone or i...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2011.09.013
更新日期:2011-12-15 00:00:00
abstract::Reduction in inspiratory capacity (IC) during exercise has been reported in chronic heart failure (CHF). Since inspiratory muscle dysfunction may be present to a variable degree, the assumption that IC reduction during exercise represents an increase in end-expiratory lung volume must be made with caution. This interp...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2018.04.005
更新日期:2018-08-01 00:00:00
abstract::The mechanisms involved in the fall of exhaled nitric oxide (NOe) concentration occurring in normal, anesthetized open chest rabbits with prolonged mechanical ventilation (MV) at low lung volume have been investigated. NOe, pH of exhaled vapor condensate, serum prostaglandin E(2), and F(2alpha), tumor necrosis factor ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2007.10.001
更新日期:2008-02-01 00:00:00
abstract::Chemoreception in fish is critical for sensing changes in the chemical composition of the external and internal environments and is often the first step in a cascade of events leading to cardiorespiratory and metabolic adjustments. Of paramount importance is the ability to sense changes in the levels of the three resp...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2015.06.007
更新日期:2016-04-01 00:00:00
abstract::Compared to mammals, little is known about the development of the respiratory control system in birds. In the present study, ventilation and metabolism were measured in Coturnix quail chicks exposed to room air, hypoxia (11 % O2), and hypercapnia (4% CO2) at 0-1, 3-4, and 6-7 days posthatching (dph). Mass-specific ven...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2020.103411
更新日期:2020-05-01 00:00:00
abstract::While the brains of most vertebrates are unable to tolerate more than a few minutes of anoxia, some freshwater turtles (Trachemys and Chrysemys), crucian carp (Carassius carassius) and frogs (Rana pipens and Rana temporaria) can survive anoxia for hours to months. Obviously, anoxia tolerance has evolved separately sev...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章,评审
doi:10.1016/j.resp.2004.03.013
更新日期:2004-08-12 00:00:00
abstract::DLCO measured in hypoxia must be corrected due to the higher affinity (increase in coefficient θ) of CO with Hb. We propose an adjustment accounting for individual changes in the equation relating DLCO to subcomponents Dm (membrane diffusive capacity) and Vc (lung capillary volume): 1/DLCO=1/Dm+1/θVc. We adjusted the ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2016.08.009
更新日期:2017-04-01 00:00:00
abstract::Recent in vitro data suggest that astrocytes may modulate respiration. To examine this question in vivo, we treated 5-day-old rat pups with methionine sulfoximine (MS), a compound that alters carbohydrate and glutamate metabolism in astrocytes, but not neurons. MS-treated pups displayed a reduced breathing frequency (...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2005.01.009
更新日期:2005-05-12 00:00:00
abstract::In obstructive sleep apnea (OSA) patients, inspiratory activation (IA) of lingual muscles protects the upper airway from collapse. We aimed to determine when rats' lingual muscles exhibit IA. In 5 Sprague-Dawley and 3 Wistar rats, we monitored cortical EEG and lingual, diaphragmatic and nuchal electromyograms (EMGs), ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2013.05.033
更新日期:2013-09-15 00:00:00
abstract::This special issue of Respiratory Physiology and Neurobiology surveys a broad range of topics focused on the neurochemical control of breathing. A variety of approaches have integrated the neurochemistry of breathing with the physiology of individual neurons, with the neuroanatomy of brainstem and forebrain respirator...
journal_title:Respiratory physiology & neurobiology
pub_type: 社论
doi:10.1016/j.resp.2008.07.021
更新日期:2008-12-10 00:00:00
abstract::We hypothesize that isoflurane and ketamine impact ventilatory pattern variability (VPV) differently. Adult Sprague-Dawley rats were recorded in a whole-body plethysmograph before, during and after deep anesthesia. VPV was quantified from 60-s epochs using a complementary set of analytic techniques that included const...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2012.12.001
更新日期:2013-02-01 00:00:00
abstract::Cardiogenic oscillations are small waves produced by heartbeats, which are superimposed on the pressure and flow signals at the airway opening. The aim of this study was to investigate the role of the two main factors believed to generate these oscillations: (1) contact between heart and lungs and (2) pulmonary blood ...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2009.04.026
更新日期:2009-07-31 00:00:00
abstract::This study tested the hypothesis that, by increasing the volume available for tidal expansion (inspiratory capacity, IC), bi-level positive airway pressure (BiPAP™) would lead to greater beneficial effects on dyspnea and exercise intolerance in comorbid heart failure (HF)-chronic obstructive pulmonary disease (COPD) t...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/j.resp.2019.04.008
更新日期:2019-08-01 00:00:00
abstract::The dependence of metabolic rate (MR) on body mass (M) is described by the general allometric equation MR=aM(b), where, a is a proportionality coefficient and b is the mass exponent. Darveau et al. [Nature 417 (2002), 166] proposed a novel 'multiple-causes' allometric cascade model as a unifying principle of the scali...
journal_title:Respiratory physiology & neurobiology
pub_type: 杂志文章
doi:10.1016/s1569-9048(03)00027-2
更新日期:2003-04-15 00:00:00