MicroRNA-328 is involved in wound repair process in human bronchial epithelial cells.

Abstract:

:Our aim was to investigate the role of microRNA on epithelial wound repair by global microRNA silencing. We have also analysed the influence of five miRNAs (miR-328, miR-342, miR-411, miR-609, miR-888, previously identified) on wound repair in 16HBE14o-bronchial epithelial cell line. Cells were transfected with siRNAs against human DROSHA and DICER1 or miRNA mimics or inhibitors. Wounding assays were performed and the cells were observed using time-lapse microscopy. The area of damage was calculated at chosen time points, followed by data analysis. Cells with silenced global miRNA expression showed a significantly slower repair rate compared to the control cells (p=0.001). For miR-328, we observed significantly delayed repair in cells transfected with the inhibitor compared to control (p=0.02). Global microRNA silencing significantly decreased the repair rate of airway epithelial cells in vitro, indicating an important role of miRNA in the regulation of wound repair and that miR-328, possibly involved in actin pathway, may be a potent modifier of this process.

authors

Narożna B,Langwinski W,Jackson C,Lackie P,Holloway JW,Szczepankiewicz A

doi

10.1016/j.resp.2017.03.010

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

59-65

eissn

1569-9048

issn

1878-1519

pii

S1569-9048(17)30095-2

journal_volume

242

pub_type

杂志文章
  • Semi-automated assessment of transdiaphragmatic pressure variability across motor behaviors.

    abstract::We developed and tested a semi-automated algorithm to generate large data sets of ventilatory information (amplitude, premotor drive and timing) across a range of motor behaviors. Adult spontaneously breathing, anesthetized mice (n = 27) underwent measurements of transdiaphragmatic pressure (Pdi) during eupnea, hypoxi...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2015.05.009

    authors: Medina-Martínez JS,Greising SM,Sieck GC,Mantilla CB

    更新日期:2015-08-15 00:00:00

  • Microglia PACAP and glutamate: Friends or foes in seizure-induced autonomic dysfunction and SUDEP?

    abstract::Seizure-induced cardiorespiratory autonomic dysfunction is a major cause of sudden unexpected death in epilepsy (SUDEP), and the underlying mechanism is unclear. Seizures lead to increased synthesis, and release of glutamate, pituitary adenylate cyclase activating polypeptide (PACAP), and other neurotransmitters, and ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2016.01.003

    authors: Bhandare AM,Kapoor K,Farnham MM,Pilowsky PM

    更新日期:2016-06-01 00:00:00

  • Pulmonary mechanics and lung histology in acute lung injury induced by Bothrops jararaca venom.

    abstract::Pulmonary mechanics [static (Est) and dynamic (Edyn) elastances, resistive (DeltaP1) and viscoelastic pressures (DeltaP2)], histology, and bronchoalveolar lavage fluid (BALF) from BALB/c mice were analysed 1, 24, 48 and 72 h after intravenous injection of saline or Bothrops jararaca crude venom [0.3 (V0.3) or 1 (V1) m...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2003.10.002

    authors: Silveira KS,Boechem NT,do Nascimento SM,Murakami YL,Barboza AP,Melo PA,Castro P,de Moraes VL,Rocco PR,Zin WA

    更新日期:2004-01-15 00:00:00

  • Redox signaling in oxygen sensing by vessels.

    abstract::In response to the increase in oxygen tension at birth, the resistance pulmonary arteries dilate, while the ductus arteriosus constricts. Although modulated by the endothelium, these opposite responses are intrinsic to the vascular smooth muscle. While still controversial, it seems likely that during normoxia the prod...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/s1569-9048(02)00054-x

    authors: Weir EK,Hong Z,Porter VA,Reeve HL

    更新日期:2002-08-22 00:00:00

  • Influence of chronic hyperoxia on the developmental time course of the hypoxic ventilatory response relative to other traits in rats.

    abstract::Newborn mammals exhibit a biphasic hypoxic ventilatory response (HVR) in which an initial increase in ventilation is followed by a decline back toward baseline levels. The magnitude of the secondary decline diminishes with postnatal age, but this transition occurs earlier in rat pups reared in moderate hyperoxia. This...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2020.103483

    authors: Pinette M,Bavis RW

    更新日期:2020-09-01 00:00:00

  • Spinal pathways mediating phrenic activation during high frequency spinal cord stimulation.

    abstract::High frequency spinal cord stimulation (HF-SCS) is a method of inspiratory muscle activation resulting in phrenic motoneuron activation via stimulation of spinal cord pathways. The specific pathways mediating this response, however, are unknown. The aim of this study was to assess the potential role of upper cervical ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2012.12.003

    authors: Dimarco AF,Kowalski KE

    更新日期:2013-03-01 00:00:00

  • Impact of sleep disordered breathing on carotid body size.

    abstract::We tested the hypotheses that: (1) carotid body size can be measured by computed tomographic angiography (CTA) with high inter-observer agreement, and (2) patients with sleep apnea exhibit larger carotid bodies than those without sleep apnea. A chart review was conducted from patients who underwent neck CTA and polyso...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2016.10.013

    authors: Welch BT,Petersen-Jones HG,Eugene AR,Brinjikji W,Kallmes DF,Curry TB,Joyner MJ,Limberg JK

    更新日期:2017-02-01 00:00:00

  • Surviving extreme lactic acidosis: the role of calcium lactate formation in the anoxic turtle.

    abstract::During prolonged anoxia at low temperature, freshwater turtles develop high plasma concentrations of both lactate and calcium. At these concentrations the formation of the complex, calcium lactate, normally of little biological significance because of the low association constant for the reaction, significantly reduce...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2004.06.020

    authors: Jackson DC

    更新日期:2004-12-15 00:00:00

  • Intermittent hypoxia-induced cardiorespiratory long-term facilitation: A new role for microglia.

    abstract::Intermittent hypoxia induces plasticity in neural networks controlling breathing and cardiovascular function. Studies demonstrate that mechanisms causing cardiorespiratory plasticity rely on intracellular signalling pathways that are activated by specific neurotransmitters. Peptides such as serotonin, PACAP and orexin...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2016.03.012

    authors: Kim SJ,Kim YJ,Kakall Z,Farnham MM,Pilowsky PM

    更新日期:2016-06-01 00:00:00

  • Recent findings in cardiovascular physiology with space travel.

    abstract::The cardiovascular system undergoes major changes in stress with space flight primarily related to the elimination of the head-to-foot gravitational force. A major observation has been that the central venous pressure is not elevated early in space flight yet stroke volume is increased at least early in flight. Recent...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2009.07.017

    authors: Hughson RL

    更新日期:2009-10-01 00:00:00

  • Sensitivity of bronchopulmonary receptors to cold and heat mediated by transient receptor potential cation channel subtypes in an ex vivo rat lung preparation.

    abstract::Changes in airway temperature can result in respiratory responses such as cough, bronchoconstriction and mucosal secretion after cold exposure and hyperventilation after heat exposure. In the present investigation, we examined the activity of bronchopulmonary receptors in response to activators of thermo-sensitive tra...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2011.05.011

    authors: Zhou Y,Sun B,Li Q,Luo P,Dong L,Rong W

    更新日期:2011-08-15 00:00:00

  • Ontogenesis of oxygen chemoreception in aquatic vertebrates.

    abstract::In aquatic vertebrates, peripheral O(2) chemoreceptors initiate compensatory physiological and behavioural responses to hypoxia, beginning at very early stages of development, to maintain sufficient gas exchange across the skin or gills. This review highlights the morphological and physiological studies, particularly ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2006.01.004

    authors: Jonz MG,Nurse CA

    更新日期:2006-11-01 00:00:00

  • Electric synapses in the carotid body-nerve complex.

    abstract::Slices of rat carotid bodies, or cultured glomus cells, were used to study intercellular coupling. This phenomenon occurs because gap junctions allow passage of currents and dyes from one cell to another. There is a two-way resistive coupling between glomus cells (GC/GC coupling), which is accompanied by activity of i...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2007.01.013

    authors: Eyzaguirre C

    更新日期:2007-07-01 00:00:00

  • Integration of hindbrain and carotid body mechanisms that control the autonomic response to cardiorespiratory and glucoprivic insults.

    abstract::Autonomic reflex responses are critical in restoring changes to circulatory factors reduced beyond the domain of homeostasis. Intermittent hypoxia triggers repeated activation of chemoreflexes, resulting in baroreflex dysfunction and widespread changes in cellular and neuronal activity regulated by sensory/motor pathw...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2018.08.008

    authors: Kakall ZM,Cohen EM,Farnham MMJ,Kim SJ,Nedoboy PE,Pilowsky PM

    更新日期:2019-07-01 00:00:00

  • Non-nutritive swallowing and respiration coordination in full-term newborn lambs.

    abstract::Swallowing is a powerful inhibitor of respiratory rhythm in infants. The present study was aimed at investigating the influence of states of alertness on non-nutritive swallowing (NNS) frequency, on NNS and respiration coordination, and on bursts of NNS frequency in newborn lambs. Six full term newborn lambs were inst...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/s1569-9048(02)00220-3

    authors: Reix P,Fortier PH,Niyonsenga T,Arsenault J,Létourneau P,Praud JP

    更新日期:2003-03-28 00:00:00

  • Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas.

    abstract::Expression of the transcription factor FOXP2 is linked to brain circuits that control motor function and speech. Investigation of FOXP2 protein expression in respiratory areas of the ponto-medullary brainstem of adult rat revealed distinct rostro-caudal expression gradients. A high density of FOXP2 immunoreactive nucl...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2018.01.014

    authors: Stanić D,Dhingra RR,Dutschmann M

    更新日期:2018-04-01 00:00:00

  • Effects of cigarette smoke and chronic hypoxia on airways remodeling and resistance. Clinical significance.

    abstract::Previously we have reported that association of cigarette smoke (CS) and chronic hypoxia (CH) interact positively to physiopathologically remodel pulmonary circulation. In present study we have exposed guinea pigs to CS smoke (four cigarettes/day; 3 months; CS) and to chronic hypoxia (12% O(2), 15 days; CH) alone or i...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2011.09.013

    authors: Olea E,Ferrer E,Prieto-Lloret J,Gonzalez-Martin C,Vega-Agapito V,Gonzalez-Obeso E,Agapito T,Peinado V,Obeso A,Barbera JA,Gonzalez C

    更新日期:2011-12-15 00:00:00

  • Low exertional inspiratory capacity is not related to dynamic inspiratory muscle weakness in heart failure.

    abstract::Reduction in inspiratory capacity (IC) during exercise has been reported in chronic heart failure (CHF). Since inspiratory muscle dysfunction may be present to a variable degree, the assumption that IC reduction during exercise represents an increase in end-expiratory lung volume must be made with caution. This interp...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2018.04.005

    authors: Plachi F,Balzan FM,Gass R,Dorneles RG,Zambiazi R,da Silva DP,Sanches PR,Clausell NO,Berton DC

    更新日期:2018-08-01 00:00:00

  • The fall in exhaled nitric oxide with ventilation at low lung volumes in rabbits: an index of small airway injury.

    abstract::The mechanisms involved in the fall of exhaled nitric oxide (NOe) concentration occurring in normal, anesthetized open chest rabbits with prolonged mechanical ventilation (MV) at low lung volume have been investigated. NOe, pH of exhaled vapor condensate, serum prostaglandin E(2), and F(2alpha), tumor necrosis factor ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2007.10.001

    authors: D'Angelo E,Koulouris NG,Della Valle P,Gentile G,Pecchiari M

    更新日期:2008-02-01 00:00:00

  • The sensing of respiratory gases in fish: Mechanisms and signalling pathways.

    abstract::Chemoreception in fish is critical for sensing changes in the chemical composition of the external and internal environments and is often the first step in a cascade of events leading to cardiorespiratory and metabolic adjustments. Of paramount importance is the ability to sense changes in the levels of the three resp...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2015.06.007

    authors: Perry SF,Tzaneva V

    更新日期:2016-04-01 00:00:00

  • Development of ventilatory chemoreflexes in Coturnix quail chicks.

    abstract::Compared to mammals, little is known about the development of the respiratory control system in birds. In the present study, ventilation and metabolism were measured in Coturnix quail chicks exposed to room air, hypoxia (11 % O2), and hypercapnia (4% CO2) at 0-1, 3-4, and 6-7 days posthatching (dph). Mass-specific ven...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2020.103411

    authors: Song MJ,Pratt AE,Bavis RW

    更新日期:2020-05-01 00:00:00

  • Vertebrate brains at the pilot light.

    abstract::While the brains of most vertebrates are unable to tolerate more than a few minutes of anoxia, some freshwater turtles (Trachemys and Chrysemys), crucian carp (Carassius carassius) and frogs (Rana pipens and Rana temporaria) can survive anoxia for hours to months. Obviously, anoxia tolerance has evolved separately sev...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章,评审

    doi:10.1016/j.resp.2004.03.013

    authors: Lutz PL,Nilsson GE

    更新日期:2004-08-12 00:00:00

  • Reappraisal of DLCO adjustment to interpret the adaptive response of the air-blood barrier to hypoxia.

    abstract::DLCO measured in hypoxia must be corrected due to the higher affinity (increase in coefficient θ) of CO with Hb. We propose an adjustment accounting for individual changes in the equation relating DLCO to subcomponents Dm (membrane diffusive capacity) and Vc (lung capillary volume): 1/DLCO=1/Dm+1/θVc. We adjusted the ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2016.08.009

    authors: Beretta E,Lanfranconi F,Grasso GS,Bartesaghi M,Alemayehu HK,Miserocchi G

    更新日期:2017-04-01 00:00:00

  • An astrocyte toxin influences the pattern of breathing and the ventilatory response to hypercapnia in neonatal rats.

    abstract::Recent in vitro data suggest that astrocytes may modulate respiration. To examine this question in vivo, we treated 5-day-old rat pups with methionine sulfoximine (MS), a compound that alters carbohydrate and glutamate metabolism in astrocytes, but not neurons. MS-treated pups displayed a reduced breathing frequency (...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2005.01.009

    authors: Young JK,Dreshaj IA,Wilson CG,Martin RJ,Zaidi SI,Haxhiu MA

    更新日期:2005-05-12 00:00:00

  • Respiratory modulation of lingual muscle activity across sleep-wake states in rats.

    abstract::In obstructive sleep apnea (OSA) patients, inspiratory activation (IA) of lingual muscles protects the upper airway from collapse. We aimed to determine when rats' lingual muscles exhibit IA. In 5 Sprague-Dawley and 3 Wistar rats, we monitored cortical EEG and lingual, diaphragmatic and nuchal electromyograms (EMGs), ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2013.05.033

    authors: Stettner GM,Rukhadze I,Mann GL,Lei Y,Kubin L

    更新日期:2013-09-15 00:00:00

  • Overview: the neurochemistry of respiratory control.

    abstract::This special issue of Respiratory Physiology and Neurobiology surveys a broad range of topics focused on the neurochemical control of breathing. A variety of approaches have integrated the neurochemistry of breathing with the physiology of individual neurons, with the neuroanatomy of brainstem and forebrain respirator...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 社论

    doi:10.1016/j.resp.2008.07.021

    authors: McCrimmon DR,Mitchell GS,Alheid GF

    更新日期:2008-12-10 00:00:00

  • Isoflurane and ketamine anesthesia have different effects on ventilatory pattern variability in rats.

    abstract::We hypothesize that isoflurane and ketamine impact ventilatory pattern variability (VPV) differently. Adult Sprague-Dawley rats were recorded in a whole-body plethysmograph before, during and after deep anesthesia. VPV was quantified from 60-s epochs using a complementary set of analytic techniques that included const...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2012.12.001

    authors: Chung A,Fishman M,Dasenbrook EC,Loparo KA,Dick TE,Jacono FJ

    更新日期:2013-02-01 00:00:00

  • Pulmonary blood flow generates cardiogenic oscillations.

    abstract::Cardiogenic oscillations are small waves produced by heartbeats, which are superimposed on the pressure and flow signals at the airway opening. The aim of this study was to investigate the role of the two main factors believed to generate these oscillations: (1) contact between heart and lungs and (2) pulmonary blood ...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2009.04.026

    authors: Tusman G,Suarez-Sipmann F,Peces-Barba G,Climente C,Areta M,Arenas PG,Bohm SH

    更新日期:2009-07-31 00:00:00

  • Effects of bi-level positive airway pressure on ventilatory and perceptual responses to exercise in comorbid heart failure-COPD.

    abstract::This study tested the hypothesis that, by increasing the volume available for tidal expansion (inspiratory capacity, IC), bi-level positive airway pressure (BiPAP™) would lead to greater beneficial effects on dyspnea and exercise intolerance in comorbid heart failure (HF)-chronic obstructive pulmonary disease (COPD) t...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/j.resp.2019.04.008

    authors: Souza A,Sperandio PA,Mazzuco A,Alencar MCN,Arbex FF,Oliveira M,Medeiros W,Rocha A,Nery LE,O Donnell DE,Neder JA

    更新日期:2019-08-01 00:00:00

  • Validity of the allometric cascade model at submaximal and maximal metabolic rates in exercising men.

    abstract::The dependence of metabolic rate (MR) on body mass (M) is described by the general allometric equation MR=aM(b), where, a is a proportionality coefficient and b is the mass exponent. Darveau et al. [Nature 417 (2002), 166] proposed a novel 'multiple-causes' allometric cascade model as a unifying principle of the scali...

    journal_title:Respiratory physiology & neurobiology

    pub_type: 杂志文章

    doi:10.1016/s1569-9048(03)00027-2

    authors: Batterham AM,Jackson AS

    更新日期:2003-04-15 00:00:00