Abstract:
:Maintenance of ex vivo hematopoietic stem cells (HSC) pool and its differentiated progeny is regulated by complex network of transcriptional factors, cell cycle proteins, extracellular matrix, and their microenvironment through an orchestrated fashion. Strides have been made to understand the mechanisms regulating in vivo quiescence and proliferation of HSCs to develop strategies for ex vivo expansion. Ex vivo expansion of HSCs is important to procure sufficient number of stem cells and as easily available source for HSC transplants for patients suffering from hematological disorders and malignancies. Our lab has established a nanofiber-based ex vivo expansion strategy for HSCs, while preserving their stem cell characteristics. Ex vivo expanded cells were also found biologically functional in various disease models. However, the therapeutic potential of expanded stem cells at clinical level still needs to be verified. This review outlines transcriptional factors that regulate development of HSCs and their commitment, genes that regulate cell cycle status, studies that attempt to develop an effective and efficient protocol for ex vivo expansion of HSCs and application of HSC in various non-malignant and malignant disorders. Overall the goal of the current review is to deliver an understanding of factors that are critical in resolving the challenges that limit the expansion of HSCs in vivo and ex vivo.
journal_name
Curr Mol Medjournal_title
Current molecular medicineauthors
Aggarwal R,Lu J,Pompili VJ,Das Hdoi
10.2174/156652412798376125subject
Has Abstractpub_date
2012-01-01 00:00:00pages
34-49issue
1eissn
1566-5240issn
1875-5666pii
BSP/CMM/E-Pub/00011journal_volume
12pub_type
杂志文章,评审abstract::A critical challenge faced by clinical nephrologists today is the escalating number of patients developing end stage renal disease, a major proportion of which is attributed to diabetic nephropathy (DN). The need for new measures to prevent and treat this disease cannot be overemphasized. To this end, modern genetic a...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524054553504
更新日期:2005-08-01 00:00:00
abstract::Our current understanding of the mechanisms of information processing and storage in the brain, based on the concept proposed more than fifty years ago by D. Hebb, is that a key role is played by changes in synaptic efficacy induced by coincident pre- and postsynaptic activity. Decades of studies of the properties of ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524023362041
更新日期:2002-11-01 00:00:00
abstract::The identification of a relevant effector of Ran GTPase (Ran) signaling and its pathways could provide a novel approach to cancer therapeutics. With recent research highlighting the significant relationship between Ran expression and the occurrence and progression of cancer, the development of a small molecule compoun...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652411797536688
更新日期:2011-11-01 00:00:00
abstract::Preeclampsia is a disease of high incidence in pregnant women which complicates pregnancy and may lead to the death of mother and baby. Preeclampsia is characterized by a series of clinical features such as hypertension and proteinuria associated with endothelial dysfunction. Although the causes of disease have not be...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/15665240113139990058
更新日期:2013-09-01 00:00:00
abstract:BACKGROUND:Seven isoforms of histone deacetylase Class III have been reported - Sirtuin (SIRT) 1-7. We recently demonstrated that EX-527, an inhibitor of SIRT1, reduces mortality in a mouse model of lethal-cecal-ligationand- puncture (CLP)-induced septic shock. Our present study was aimed at determining whether selecti...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/156652401507150903185852
更新日期:2015-01-01 00:00:00
abstract::Bulimia nervosa (BN) and Anorexia Nervosa (AN) are currently classified as eating disorders (ED). Both disorders are the product of complex interaction between physiological and psychological and social processes; they are characterized by abnormal eating behavior. However, patients with BN differ from AN in their nut...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524033479762
更新日期:2003-06-01 00:00:00
abstract::There has been prolonged and significant interest in manipulating the genome for a wide range of applications in biomedical research and medicine. An existing challenge in realizing this potential has been the inability to precisely edit specific DNA sequences. Past efforts to generate targeted double stranded DNA cle...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524016666160316150847
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is the third most common cancer worldwide and the third leading cause of cancer-related death. It is a heterogeneous disease that develops through different genetic and epigenetic mechanisms. To date, no comprehensive systematic review investigating genetic risk factors for familial a...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524019666191014170136
更新日期:2020-01-01 00:00:00
abstract::Conformational or misfolding diseases are a large class of devastating human disorders associated with protein misfolding and aggregation. Most conformational diseases are caused by a combination of genetic and environmental factors, suggesting that spontaneous events can destabilize the protein involved in the pathol...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408783565595
更新日期:2008-02-01 00:00:00
abstract::Induced pluripotent stem (iPS) cell research has been growing a new height throughout the world due to its potentialities in medical applications. We can explore several therapeutic applications through the iPS cell research. In this review, we have first discussed the development of iPS cells, reprogramming factors, ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652410793384178
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:In vertebrates, cilium is crucial for Hedgehog signaling transduction. Forkhead box transcriptional factor FoxF1 is reported to be associated with Sonic Hedgehog (Shh) signaling in many cases. However, the role of FoxF1 in cilium remains unknown. Here, we showed an essential role of FoxF1 in the regulation o...
journal_title:Current molecular medicine
pub_type: 信件
doi:10.2174/1566524019666190405115420
更新日期:2019-01-01 00:00:00
abstract::Nitric oxide has emerged as one of the most important and diverse players in physiology. This small diatomic radical stunned researchers because of its existence and unique biological properties in human physiology. Over the last two decades it was found that NO often has fickle behavior in pathophysiological mechanis...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524043359854
更新日期:2004-11-01 00:00:00
abstract::Human induced pluripotent stem cells (hiPSCs) have great potential as a robust source of progenitors for regenerative medicine. The novel technology also enables the derivation of patient-specific cells for applications to personalized medicine, such as for personal drug screening and toxicology. However, the biologic...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524011313050010
更新日期:2013-06-01 00:00:00
abstract::Although it has been known for long time that atherosclerosis is associated with lipid deposition, only recently it has been accepted that the plasmatic concentration of cholesterol, especially LDL cholesterol, is a risk factor for atherosclerosis. However, chemically modified LDL, but not native LDL, is able to induc...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524023362177
更新日期:2002-09-01 00:00:00
abstract::Eicosanoids are diverse mediators of inflammation that derive from a single cell membrane phospholipid-associated precursor, arachidonic acid. This precursor is metabolized to several groups of lipid mediators, including (but not limited to) prostaglandins, leukotrienes, and lipoxins, in a tightly regulated, coordinat...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652408785160989
更新日期:2008-08-01 00:00:00
abstract::A framework was developed to provide an integrated view of mycobacterial growth and its regulation. The topics reviewed include the properties of cell cultures and their relation to properties of individual cells, cell sizes and macromolecular compositions, uptake of nutrients through the cell envelope, protein biosyn...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652407780598584
更新日期:2007-05-01 00:00:00
abstract::T cells, as they develop in the thymus come to express antigen receptors. The specificity of these receptors cannot be predicted and must include many with potential anti-self reactivity. Those that encounter self-antigens, in association with self-MHC (major histocompatibility complex), with high affinity are inactiv...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524013363573
更新日期:2001-09-01 00:00:00
abstract::Strong epidemiological evidence indicates that migraine, especially migraine with aura, is associated with increased risk of ischemic stroke. However, the precise mechanisms of such a relation are currently not fully elucidated and are still a matter of speculation. Migraine may directly cause an ischemic event (i.e, ...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409787581583
更新日期:2009-03-01 00:00:00
abstract::Deregulation of the PI3K pathway is common in human cancer. The basic players in this pathway are the kinases PI3K and AKT and the phosphatase PTEN. This review will summarize some of the key animal models that have helped us understand this signaling network and its contribution to tumorigenesis. Recently, great adva...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652409788488766
更新日期:2009-06-01 00:00:00
abstract::Pancreatic and duodenal homeobox-1 (PDX-1) is a homeodomain-containing transcription factor that plays a critical role in pancreatic development, β-cell differentiation, maintenance of normal β-cell function and tumorigenesis. PDX-1 is subjected to extensive post-translational modifications for its stability, subcellu...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
abstract::Pancreatic Adenocarcinoma (PDAC) is one of the most deadly malignant tumors worldwide. A variety of mechanisms are involved in PDAC biological behaviors, of which, the mechanisms of immune escape may be a pivotal hallmark. HLA-G is a tolerant molecule implicated in tumor escape and serves as a prognostic biomarker in ...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524015666150401102218
更新日期:2015-01-01 00:00:00
abstract::Peroxisomal proliferator activated receptor gamma (PPARgamma) belongs to the family of nuclear hormone receptors (NHRs), which directly regulate transcription of target genes. The regulatory role of this receptor on lipid metabolism and insulin sensitization is well established. Recently, the overexpression of this re...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/156652407781695765
更新日期:2007-09-01 00:00:00
abstract::Fibromyalgia (FM) is a prevalent disorder defined by the presence of chronic widespread pain in association with fatigue, sleep disturbances and cognitive dysfunction. Recent studies indicate that bipolar spectrum disorders frequently co-occur in individuals with FM. Furthermore, shared pathophysiological mechanisms a...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524016666160126144027
更新日期:2016-01-01 00:00:00
abstract::Carcinoma-associated fibroblasts (CAFs) are activated fibroblasts during development of several cancer types, and therefore emerge as an attractive therapeutic target for cancer therapy. Drugs such as PT-100 and sibrotuzumab that block the functions of CAFs have already been in clinical trials. However, these drugs ex...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524018666171205113959
更新日期:2017-01-01 00:00:00
abstract::Immune tolerance can be induced by numerous methods. This review article aims to draw lines of similarity and contrast between two unique models of immune tolerance, namely Anterior Chamber Associated Immune Deviation (ACAID) and Nickel-induced oral tolerance. ACAID is an immune tolerance model that leads to the gener...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524015666150114114602
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND & OBJECTIVE:Interleukin 37 (IL-37) is an important regulator of the anti-inflammatory T-cell response. In this study, we investigated its expression and function in peripheral blood mononuclear cells (PBMCs) of patients with HLA-B27 associated acute anterior uveitis (AAU) and idiopathic AAU. METHODS:15 pati...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524018666180207152941
更新日期:2018-03-09 00:00:00
abstract:BACKGROUND:Inclusion of anticancer drugs into biocompatible nanoparticulate carriers decreases the general toxicity and improves the efficacy of clinical treatments due to the reduction of soluble circulating free drug. METHODS:In addition, removal of emerging drug contaminants from wastewaters is a necessity that sho...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524021666210111104428
更新日期:2021-01-10 00:00:00
abstract::The generation of functional neural progenitor cells (NPCs) independent of donor brain tissue and embryonic tissues is of great therapeutic interest with regard to regenerative medicine and the possible treatment of neurodegenerative disorders. Traditionally, NPCs are derived through the differentiation of embryonic s...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/156652412798889018
更新日期:2012-02-01 00:00:00
abstract::Type 2 diabetes carries a 2-6-fold increased risk of cardiovascular disease (CVD) and death. Indeed, the risk of major cardiovascular events in Type 2 diabetic patients without history of coronary heart disease (CHD) is equivalent to that observed in non-diabetic subjects with CHD. However, atherosclerosis may also pr...
journal_title:Current molecular medicine
pub_type: 杂志文章,评审
doi:10.2174/1566524053766095
更新日期:2005-05-01 00:00:00
abstract:PURPOSE:A small molecular compound, aminooxy-acetic acid (AOA), has been shown to modulate experimental autoimmune encephalomyelitis (EAE). The current study was designed to investigate whether AOA has a similar effect on the development of experimental autoimmune uveitis (EAU) and to further explore underlying mechani...
journal_title:Current molecular medicine
pub_type: 杂志文章
doi:10.2174/1566524020666200211112219
更新日期:2020-01-01 00:00:00