Epigenome characterization at single base-pair resolution.

abstract：:Most, if not all, peptide- and protein-based hydrogels formed by self-assembly can be characterized as kinetically trapped 3D networks of fibrils. The propensity of disease-associated amyloid-forming peptides and proteins to assemble into polymorphic fibrils suggests that cross-β fibrils comprising hydrogels may also ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagy-Smith K,Moore E,Schneider J,Tycko R

更新日期：2015-08-11 00:00:00

abstract：:A method is presented for starting from a very-low-energy high-dimensional conformation and obtaining a low-energy three-dimensional structure by gradual contraction of the dimensionality. The contraction in dimensionality is achieved by use of Cayley-Menger determinants, of which a simplified form is derived here. Pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Purisima EO,Scheraga HA

更新日期：1986-05-01 00:00:00

abstract：:The ionophores A23187 and bromolasalocid (Br-X537A) acted synergistically in translocating Ca(2+) from an aqueous into an organic immiscible phase or in mediating Ca(2+) transport across the organic phase, the effects obtained in the simultaneous presence of both ionophores being greater than those expected from a sum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Deleers M,Gelbcke M,Malaisse WJ

更新日期：1981-01-01 00:00:00

abstract：:The emergence of antibiotic resistance among nosocomial pathogens has reemphasized the need for effective infection control strategies. The spread of resistant pathogens within hospital settings proceeds along various routes of transmission and is characterized by large fluctuations in prevalence, which are typical fo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pelupessy I,Bonten MJ,Diekmann O

更新日期：2002-04-16 00:00:00

abstract：:Smooth muscle cells use fibronectin to bind to type I and type III collagens but bind to type V collagen by a trypsin-resistant intrinsic glycoconjugate. The binding site on type V collagen is located in the alpha A chain. By using collagen-coated filters in a modified Boyden chamber assay for chemotaxis, it was obser...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grotendorst GR,Seppä HE,Kleinman HK,Martin GR

更新日期：1981-06-01 00:00:00

abstract：:Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Busbee DL,Joe CO,Norman JO,Rankin PW

更新日期：1984-09-01 00:00:00

abstract：:In the manufacture of chemical feedstocks and subsequent processing into derivatives and materials, the U.S. chemical industry sets the current standard of excellence for technological competitiveness. This world-class leadership is attributed to the innovation and advancement of chemical engineering process technolog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell JW

更新日期：1992-02-01 00:00:00

abstract：:The p21 region of human chromosome 9 is thought to contain a gene (MLM) involved in genetic susceptibility to melanoma and a gene or genes that influence progression of certain other tumors. Genomic clones that span a large region in 9p21 surrounding the presumptive tumor suppressor gene(s) have been isolated. A set o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weaver-Feldhaus J,Gruis NA,Neuhausen S,Le Paslier D,Stockert E,Skolnick MH,Kamb A

更新日期：1994-08-02 00:00:00

abstract：:Heparan sulfate regulates diverse cell-surface signaling events, and its roles in the development of the nervous system recently have been increasingly uncovered by studies using genetic models carrying mutations of genes encoding enzymes for its synthesis. On the other hand, the role of heparan sulfate in the physiol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irie F,Badie-Mahdavi H,Yamaguchi Y

更新日期：2012-03-27 00:00:00

abstract：:The RIG-G gene, originally isolated from an acute promyelocytic leukemia cell line NB4, codes for a 60-kDa cytoplasmic protein that is induced by all-trans retinoic acid (ATRA) treatment along with the induction of morphological differentiation of NB4 cells. Here, we provide evidence that ectopic expression of Rig-G i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao S,Li D,Zhu HQ,Song MG,Pan XR,Jia PM,Peng LL,Dou AX,Chen GQ,Chen SJ,Chen Z,Tong JH

更新日期：2006-10-31 00:00:00

abstract：:Examination of old human lenses indicates that low-molecular-weight alpha crystallin is not present in the inner 30-40%, the nucleus, of the lens. The remainder of such lenses, the periphery, contains normal levels of this protein. This finding is in marked contrast to observations in young lenses, where a large quant...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roy D,Spector A

更新日期：1976-10-01 00:00:00

abstract：:3-Hydroxykynurenine (3-HK) is a tryptophan metabolite whose level in the brain is markedly elevated under several pathological conditions, including Huntington disease and human immunodeficiency virus infection. Here we demonstrate that micromolar concentrations (1-100 microM) of 3-HK cause cell death in primary neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okuda S,Nishiyama N,Saito H,Katsuki H

更新日期：1996-10-29 00:00:00

abstract：:Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Putter I,Barreto A,Markley JL,Jardetzky O

更新日期：1969-12-01 00:00:00

abstract：:The excitatory neurotransmitter, glutamate, activates N-methyl-d-aspartate (NMDA) receptors to induce long-lasting synaptic changes through alterations in gene expression. It is believed that these long-lasting changes contribute to learning and memory, drug tolerance, and ischemic preconditioning. To identify NMDA-in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hong SJ,Li H,Becker KG,Dawson VL,Dawson TM

更新日期：2004-02-17 00:00:00

abstract：:Partial, unilateral nigrostriatal lesions of varying severity were produced in rats by injecting graded doses of 6-hydroxydopamine into the substantia nigra. Formation of the dopamine metabolites dihydroxyphenylacetic acid and homovanillic acid in each surviving nigrostriatal neuron (estimated by the ratios of dihydro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Melamed E,Hefti F,Wurtman RJ

更新日期：1980-07-01 00:00:00

abstract：:Despite nearly four decades of effort, broad inhibition of oncogenic RAS using small-molecule approaches has proven to be a major challenge. Here we describe the development of a pan-RAS biologic inhibitor composed of the RAS-RAP1-specific endopeptidase fused to the protein delivery machinery of diphtheria toxin. We s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vidimar V,Beilhartz GL,Park M,Biancucci M,Kieffer MB,Gius DR,Melnyk RA,Satchell KJF

更新日期：2020-07-21 00:00:00

abstract：:Recent studies have indicated that mammalian cells contain a cytosolic protein disaggregation machinery comprised of Hsc70, DnaJ homologs, and Hsp110 proteins, the last of which acts to accelerate a rate-limiting step of nucleotide exchange of Hsc70. We tested the ability of transgenic overexpression of a Thy1 promote...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagy M,Fenton WA,Li D,Furtak K,Horwich AL

更新日期：2016-05-10 00:00:00

abstract：:The nerve growth factor protein was purified over 100-fold from adult mouse salivary glands. The first step was a gel filtration on Sephadex G-100 at pH 7.5 of the aqueous gland extract. After gel filtration, most of the NGF activity was eluted in the 80,000-90,000-molecular-weight region (G-100 pool). The G-100 pool ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bocchini V,Angeletti PU

更新日期：1969-10-01 00:00:00

abstract：:Expanded hexanucleotide repeats in the chromosome 9 open reading frame 72 (C9orf72) gene are the most common genetic cause of ALS and frontotemporal degeneration (FTD). Here, we identify nuclear RNA foci containing the hexanucleotide expansion (GGGGCC) in patient cells, including white blood cells, fibroblasts, glia, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lagier-Tourenne C,Baughn M,Rigo F,Sun S,Liu P,Li HR,Jiang J,Watt AT,Chun S,Katz M,Qiu J,Sun Y,Ling SC,Zhu Q,Polymenidou M,Drenner K,Artates JW,McAlonis-Downes M,Markmiller S,Hutt KR,Pizzo DP,Cady J,Harms MB,

更新日期：2013-11-19 00:00:00

abstract：:The cancer stem cell hypothesis posits that tumors are derived from a single cancer-initiating cell with stem cell properties. The task of identifying and characterizing a single cancer-initiating cell with stem cell properties has proven technically difficult because of the scarcity of the cancer stem cells in the ti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zucchi I,Sanzone S,Astigiano S,Pelucchi P,Scotti M,Valsecchi V,Barbieri O,Bertoli G,Albertini A,Reinbold RA,Dulbecco R

更新日期：2007-06-19 00:00:00

abstract：:In this article, a mechanism of arsenite [As(III)]resistance through methylation and subsequent volatization is described. Heterologous expression of arsM from Rhodopseudomonas palustris was shown to confer As(III) resistance to an arsenic-sensitive strain of Escherichia coli. ArsM catalyzes the formation of a number ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qin J,Rosen BP,Zhang Y,Wang G,Franke S,Rensing C

更新日期：2006-02-14 00:00:00

abstract：:The role of global climate change in the decline of biodiversity and the emergence of infectious diseases remains controversial, and the effect of climatic variability, in particular, has largely been ignored. For instance, it was recently revealed that the proposed link between climate change and widespread amphibian...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rohr JR,Raffel TR

更新日期：2010-05-04 00:00:00

abstract：:We identified the phosphatidylinositol transfer protein (PITP) as being responsible for a powerful latent, nucleotide-independent, Golgi-vesiculating activity that is present in the cytosol but is only manifested as an uncontrolled activity in a cytosolic protein subfraction, in which it is separated from regulatory c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Simon JP,Morimoto T,Bankaitis VA,Gottlieb TA,Ivanov IE,Adesnik M,Sabatini DD

更新日期：1998-09-15 00:00:00

abstract：:Nerve growth factor (NGF) is a neurotrophin with the ability to exert specific effects on cells of the immune system. Human monocytes/macrophages (M/M) infected in vitro with HIV type 1 (HIV-1) are able to produce substantial levels of NGF that are associated with enhanced expression of the high-affinity NGF receptor ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garaci E,Caroleo MC,Aloe L,Aquaro S,Piacentini M,Costa N,Amendola A,Micera A,Caliò R,Perno CF,Levi-Montalcini R

更新日期：1999-11-23 00:00:00

abstract：:The Clausius-Mossotti function for the dielectric constant is expanded in terms of single atom and pair polarizabilities, leading to contributions that depend on both the trace and the anisotropy of the pair-polarizability tensor. The short-range contribution of the anisotropic part to the pair polarizabilities has pr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alder BJ,Beers JC,Strauss HL,Weis JJ

更新日期：1980-06-01 00:00:00

abstract：:Island biogeography theory posits that species richness increases with island size and decreases with isolation. This logic underpins much conservation policy and regulation, with preference given to conserving large, highly connected areas, and relative ambivalence shown toward protecting small, isolated habitat patc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wintle BA,Kujala H,Whitehead A,Cameron A,Veloz S,Kukkala A,Moilanen A,Gordon A,Lentini PE,Cadenhead NCR,Bekessy SA

更新日期：2019-01-15 00:00:00

abstract：:Calcium sensitivity of myosin cross-bridge activation in striated muscles commonly varies during ontogeny and in response to alterations in muscle usage, but the consequences for whole-organism physiology are not well known. Here we show that the relative abundances of alternatively spliced transcripts of the calcium ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marden JH,Fitzhugh GH,Wolf MR,Arnold KD,Rowan B

更新日期：1999-12-21 00:00:00

abstract：:A rapid, simple, and quantitative filter-binding assay using glass fiber filters has been developed to detect the convalent adenovirus DNA-terminal protein complex. The assay is unusually sensitive because binding of protein-free DNA generally is less than 0.1%. Binding of the adenovirus complex to filters is mediated...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coombs DH,Pearson GD

更新日期：1978-11-01 00:00:00

abstract：:We present evidence that the JAK-STAT signal transduction pathway regulates multiple developmental processes in Drosophila. We screened for second-site mutations that suppress the phenotype of the hyperactive hopTum-1 Jak kinase, and recovered a mutation that meiotically maps to the known chromosomal position of D-Sta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan R,Luo H,Darnell JE Jr,Dearolf CR

更新日期：1996-06-11 00:00:00

abstract：:The Bacillus subtilis phage SP01, whose DNA contains 5-hydroxymethyluracil (hmUra) in place of thymine, codes for an abundant, small, basic protein called TF1. TF1 binds preferentially to hydroxymethyluracil-containing DNA and thereby selectively inhibits transcription of such DNA in vitro. The gene for TF1 has been s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene JR,Brennan SM,Andrew DJ,Thompson CC,Richards SH,Heinrikson RL,Geiduschek EP

更新日期：1984-11-01 00:00:00