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Filter-binding assay for covalent DNA-protein complexes: adenovirus DNA-terminal protein complex.

Abstract：

:A rapid, simple, and quantitative filter-binding assay using glass fiber filters has been developed to detect the convalent adenovirus DNA-terminal protein complex. The assay is unusually sensitive because binding of protein-free DNA generally is less than 0.1%. Binding of the adenovirus complex to filters is mediated by terminal protein. We have found that: (i) the adenovirus complex binds maximally to filters in NaCl at concentrations higher than 0.2 M; (ii) noncovalent complexes between protein-free DNA and adenovirus proteins bind to filters in salt at concentrations lower than 0.4 M but not in concentrations higher than 0.7 M; and (iii) protein-free DNA alone binds to filters in guanidine.hydrochloride at concentrations higher than 0.8 M. By varying the ionic conditions, "all or none" modulation of these interactions can be achieved.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Coombs DH,Pearson GD

doi

10.1073/pnas.75.11.5291

subject

Has Abstract

pub_date

1978-11-01 00:00:00

pages

5291-5

issue

11

eissn

0027-8424

issn

1091-6490

journal_volume

75

pub_type

杂志文章

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