Abstract:
:Although mesenchymal stem cells (MSCs) are considered to be the cells of origin for most sarcomas, the role of MSCs as a source of tumor stroma is not fully understood in this tumor type. The current study investigated whether MSCs affect the tumor growth and metastatic ability in rat osteosarcoma model. Results from subcutaneous co-implantation of rat osteosarcoma COS1NR cells, established in our laboratory, with rat MSCs isolated from femur bone marrow showed that the incidence of tumor formation and tumor growth rate was higher until 5 weeks compared to COS1NR cell inoculation alone. However, no difference was observed in tumor growth afterwards and in the number of metastatic nodules at 9 weeks (0.75 vs. 1.2). Intravenous MSC injection at weeks 3 and 5 after subcutaneous inoculation of COS1NR cells significantly increased the number of lung nodules in the group with MSC injection compared to the group without MSC injection (17.33 vs. 2.0), while no difference was observed in subcutaneous tumor growth between those groups. Pathway analysis from gene expression profile identified that genes involved in focal adhesion, cytokine-cytokine receptor and extracellular matrix-receptor pathways such as CAMs (ICAM and VCAM)-integrins were highly expressed in MSCs, possibly participating in the tumor progression of osteosarcoma. These results suggest that MSCs could provide a source of microenvironments for osteosarcoma cells, and might enhance the ability of settlement and colonization which lead to early onset of growth and metastasis, possibly through their activated pathways interaction.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Tsukamoto S,Honoki K,Fujii H,Tohma Y,Kido A,Mori T,Tsujiuchi T,Tanaka Ydoi
10.3892/ijo.2011.1220subject
Has Abstractpub_date
2012-01-01 00:00:00pages
163-9issue
1eissn
1019-6439issn
1791-2423journal_volume
40pub_type
杂志文章abstract::Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fractions from a novel st...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000774
更新日期:2010-11-01 00:00:00
abstract::CD34 is commonly used as an endothelial cell marker of tumor vessels. However, this marker detects not only newly formed, but also pre-existing large blood vessels. Nestin, a class VI intermediate filament protein, has recently received attention as a marker for detecting newly formed endothelial cells. In this study,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::Lack of life-prolonging therapies has provided much of the impetus for seeking complementary and alternative management/treatment options by prostate cancer (CaP) patients. Among these, the use of dietary supplements and botanical products has been showing a sustained increase in recent years, owing in part to some en...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::Periostin (POSTN) is a protein involved in multiple processes important for cancer development, both at the stage of cancer initiation and progression, as well as metastasis. The aim of this study was to determine the expression of POSTN in the cells of non-invasive ductal breast carcinoma in situ (DCIS) and invasive ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4109
更新日期:2017-10-01 00:00:00
abstract::In recent years major progress has been made in understanding the molecular steps of death receptor and mitochondrial pathway of apoptosis. Mechanisms of NF-kappaB-mediated survival (anti-apoptotic) signal have been delineated. A novel anti-apoptotic mechanism of FLIP in the recruitment of RIP and TRAF-1 and TRAF-2 to...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2003-01-01 00:00:00
abstract::Cognate interactions between naïve tumor antigen (TA)-specific T cells and TA-presenting dendritic cells (DCs) are facilitated by secondary lymphoid organs such as lymph nodes or the spleen. These can result either in TA-specific tolerance or, depending on environmental costimulatory signals, in TA-specific immune res...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2015.3197
更新日期:2015-12-01 00:00:00
abstract::Delivery of electric pulses to an established solid tumor augments the permeability of cell membrane and increases the susceptibility of tumors to an anti-cancer agent that is administered in the vicinity of tumors. Forced expression of the wild-type p53 gene in tumor cells that have non-functional p53 gene(s) can als...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.4.825
更新日期:2001-04-01 00:00:00
abstract::Prevention of chemotherapy induced nausea and vomiting in an outpatient population requires an antiemetic regimen which is reliable and safe and easy to administer. In this study the use of oral ondansetron was investigated in 45 patients receiving 143 courses of non-cisplatin containing chemotherapy regimens on an ou...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.6.1283
更新日期:1994-06-01 00:00:00
abstract::Cholangiocarcinoma is the most common biliary duct malignancy and the second most common primary liver cancer, accounting for 10‑20% of hepatic malignancies. With high mortality and poor prognosis, the 5‑year survival rate of cholangiocarcinoma is only 10%. A previous study demonstrated a significant association betwe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5165
更新日期:2020-12-30 00:00:00
abstract::PTEN, a gene encoding a dual specificity phosphatase, is frequently altered in endometrial carcinoma. Moreover, these alterations are observed even in atypical hyperplasia of the endometrium. This evidence suggests that mutation of PTEN is an early genetic alteration involved in endometrial carcinogenesis. Adenovirus-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.6.1069
更新日期:1999-12-01 00:00:00
abstract::Sentinel node (SN) mapping and biopsy is a procedure that accurately stages the regional lymph node (LN) basin. Defined patterns of lymphatic drainage allow intraoperative determination of the first (sentinel) lymph node in the regional basin, and the absence of metastatic disease in the SN accurately reflects the abs...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.15.1.25
更新日期:1999-07-01 00:00:00
abstract::We hypothesize that dysregulated expression levels of the developmental regulatory genes in the adult body result in tumor development and malignant progression. PAX genes discovered as human orthologous genes of Drosophila 'paired' encode transcription factors, which control the expression of target genes to go on al...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Although a number of recent studies have reported the involvement of leptin in colorectal carcinogenesis, findings are contradictory and difficult to interpret. Our group has previously reported that leptin signaling might have an important role in the development of colorectal adenomas. In this study, we investigated...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1105
更新日期:2011-10-01 00:00:00
abstract::Radioiodinated meta-iodobenzylguanidine (MIBG), an analogue of norepinephrine, has been used in management of neuroendocrine tumors. Recent studies reveal that distribution of radioiodinated MIBG in animals depends on the specific activity of this radiopharmaceutical. In order to clarify the effect of specific activit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.815
更新日期:1997-04-01 00:00:00
abstract::Glycodelin [gene name, progesterone associated endometrial protein (PAEP)] was initially described as an immune system modulator in reproduction. Today, it is also known to be expressed in several types of cancer, including non‑small cell lung cancer (NSCLC). In this cancer type, the feasibility of its usage as a foll...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4654
更新日期:2019-02-01 00:00:00
abstract::Cutaneous infantile hemangioma progresses through proliferation and involution phases. Since treatment with interferon, a negative regulator of angiogenesis, accelerates the involution phase, we hypothesized that cutaneous infantile hemangioma is associated with an imbalance between endogenous positive and negative re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.3.401
更新日期:1999-03-01 00:00:00
abstract::Although many chemotherapies have been developed for melanomas, successful therapy would be aided by the identification of intrinsic mechanisms that are crucial for melanoma survival. Here, we used resveratrol, a phytoalexin, as an anti-melanoma reagent. Applying resveratrol to various human and murine melanoma cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2480
更新日期:2014-08-01 00:00:00
abstract::Because K-Ras mutation and cyclooxygenase-2 (COX-2) overexpression are hallmarks of majority of pancreatic cancer patients, an approach to inhibit the progression and growth of pancreatic cancer using the simultaneous administration of agents that inhibit the function of both targets, should be considered. In the pres...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2350
更新日期:2014-06-01 00:00:00
abstract::MicroRNA‑301a (miRNA/miR‑301a) and nuclear factor (NF)‑κB signaling play important roles in tumor invasion, migration and progression. However, the role of miRNA‑301a‑3p in human gastric cancer (GC), and specifically in the activation of NF‑κB signaling, remains unclear. The aim of the present study was to investigate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5072
更新日期:2020-08-01 00:00:00
abstract::Patients with long-standing ulcerative colitis (UC) have higher risk of developing colorectal cancer. Albeit the causes remain to be understood, epigenetic alterations have been suggested to play a role in the long-term cancer risk of these patients. In this work, we developed a novel microarray platform based on meth...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1283
更新日期:2012-04-01 00:00:00
abstract::Diosmetin, a plant flavonoid, has been shown to exert promising effects on prostate cancer cells as an anti‑proliferative and anticancer agent. In this study, using western blot analysis for protein expression and flow cytometry for cell cycle analysis, we determined that the treatment of the LNCaP and PC‑3 prostate c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4407
更新日期:2018-08-01 00:00:00
abstract::The exact mechanism behind the effect of hypoxia-inducible factor-1alpha (HIF-1alpha) on the proliferation and/or apoptosis of carcinoma cells is still a matter of debate. We treated a human gastric carcinoma cell line, MKN-1 (mutant P53), with 500 microM CoCl(2). A dual-phase pattern of HIF-1alpha expression with an ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-09-01 00:00:00
abstract::Recent studies have demonstrated that natural antisense transcripts, which are complementary sequences to messenger RNA, have important cellular functions such as the stabilization and silencing of mRNA. However, the possible contribution of antisense transcripts in hepatocellular c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1382
更新日期:2012-06-01 00:00:00
abstract::Accumulating evidence has demonstrated the crucial roles of long non‑coding RNAs (lncRNAs) in various human cancers, including non‑small cell lung cancer (NSCLC). However, to the best of our knowledge, the role of the lncRNA cancer susceptibility candidate 7 (CASC7) in NSCLC has not been clearly determined. The aim of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5076
更新日期:2020-08-01 00:00:00
abstract::Due to the central role in predicting response to herceptin and possibly also other anticancer drugs, accurate and reproducible detection of the HER2 status is important. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are the most commonly used methods for HER2 analysis. It is a disadvantage ...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::Inositol hexaphosphate (IP6), also known as phytic acid, has been shown to exhibit anticancer effects in a number of preclinical tumor models. IP6 decreases proliferation by arresting cells in the G0/G1 phase, inhibits iron-mediated oxidative reactions, enhances differentiation and stimulates apoptosis. The present st...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4488
更新日期:2018-10-01 00:00:00
abstract::alpha-tocopheryl phosphate (TocP) has been elucidated for diverse effectiveness through unequivocal mechanisms independent of its conversion to alpha-tocopherol (Toc). The present study showed that TocP, in a dose-dependent manner, repressed invasion of human fibrosarcoma HT-1080 cells through the basement membrane, m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000445
更新日期:2009-12-01 00:00:00
abstract::Hsp90α is a molecular chaperone protein involved in the structural maturation of oncogenic signaling proteins. Hsp90 was recently identified as an anticancer target; various studies are ongoing to find ways for managing cancer through Hsp90α. However, this approach is limited by reported side-effects. Hypoxia is a hal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1450
更新日期:2012-07-01 00:00:00
abstract::ETS variant 1 (ETV1) is an oncogenic transcription factor. However, its role in colorectal cancer has remained understudied. The present study demonstrated that ETV1 downregulation led to reduced HCT116 colorectal cancer cell growth and clonogenic activity. Furthermore, the ETV1 mRNA levels were enhanced in colorectal...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
abstract::Three separate experiments, designed to minimize the DNA repair process following exposures to mutagens, were performed to determine whether DNA repair capability is the sole factor responsible for differential mutagen sensitivity. These experiments included (i) very short bleomycin pulse treatments (2, 5 and 10 min),...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.51
更新日期:1995-01-01 00:00:00