Abstract:
:Secondary metabolites from actinomycetes especially the genus Streptomyces may be one of the most important sources for novel anticancer agents. A purified fraction from a novel actinomycete strain, Streptomyces sp. H7372, was elucidated in breast cancer cells. We have isolated three purified fractions from a novel strain, Streptomyces sp. H7372. One of the fractions, designated as 31-2, exhibited the strongest growth-inhibitory effect and thereby was selected for further studies. 31-2 exerted a growth-inhibitory effect on a panel of 15 human cancer and 2 non-malignant cell lines. In MCF-7 and MDA-MB-231 breast cancer cells, 31-2 induced a cytostatic (anti-proliferative) effect without causing cytotoxicity (cell death). Our data suggest that the cytostasis resulted from cell cycle arrest at the G1 phase in MCF-7 cells and at the S phase in MDA-MB-231 cells. Western blot analysis demonstrated a modulation of phosphorylation of the Rb and CDC2 proteins and of CDK4, cyclin D1 and cyclin D3 in the 31-2-treated breast cancer cell lines. The protein levels of CDK2, CDK6, and PCNA were not affected by 31-2 treatment. 31-2 also exhibited an anti-invasive effect in MDA-MB-231 cells. However, this effect is not attributed to the modulation of proteolytic activity in MDA-MB-231 cells as the enzymatic degradation of type IV collagen was not affected by 31-2. The 31-2 is a potent cytostatic and anti-invasive agent and modulates the cell cycle pathway. Together, these results will have important implications in searching for novel approaches to treat cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Yip WK,Cheenpracha S,Chang LC,Ho CC,Seow HFdoi
10.3892/ijo_00000774subject
Has Abstractpub_date
2010-11-01 00:00:00pages
1229-41issue
5eissn
1019-6439issn
1791-2423journal_volume
37pub_type
杂志文章abstract::Galiximab is a primatized monoclonal antibody that targets CD80 expressed on malignant B cells and is being studied in the clinic as a potential treatment for follicular NHL. We have recently reported that galiximab signals B-NHL cells in vitro and inhibits cell growth and sensitizes resistant tumor cells to apoptosis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1986
更新日期:2013-08-01 00:00:00
abstract::Following the publication of this article, the authors noted that there was an error in the affiliations. Specifically, the affiliation of the first author JUAN XU should be: Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China. [the original article was publis...
journal_title:International journal of oncology
pub_type: 杂志文章,已发布勘误
doi:10.3892/ijo.2017.3977
更新日期:2017-06-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway is preferentially activated in diffuse-type gastric cancer (GC) compared with intestinal-type GC; however, histological typing is currently the only method for distinguishing these two major types of GC. We compared t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2387
更新日期:2014-06-01 00:00:00
abstract::The aim of the present study was to evaluate the performance of whole-body diffusion-weighted imaging (WB-DWI), whole-body positron emission tomography with computed tomography (WB-PET/CT), and whole-body positron emission tomography with magnetic resonance imaging (WB-PET/MRI) in staging patients with untreated invas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4012
更新日期:2017-07-01 00:00:00
abstract::Endoscopic examination with iodine staining has led to the easy detection of multiple superficial esophageal carcinoma (MSEC). The purpose of this study was to better understand the characteristics of MSEC. Of 49 patients with multiple esophageal carcinomas, 19 had superficial carcinoma. Multiple esophageal carcinomas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.2.421
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abstract::MicroRNAs (miRNAs) were reported to be involved in the development of clear cell renal cell carcinoma (ccRCC). However, the study on miRNAs in ccRCC is far from complete. The present study identified miRNAs which could act as potential novel prognostic markers for ccRCC, and analyzed its possible mechanism. We found t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3746
更新日期:2016-12-01 00:00:00
abstract::The GTPase activity of purified dimeric tubulin (alpha+beta) at 5 mu M was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB-231 (human mammary cancer) cells migrating through 12-mu m pores was inhibited by DIME similar...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1247
更新日期:1997-12-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.5.1061
更新日期:1994-05-01 00:00:00
abstract::The deregulation of the epidermal growth factor receptor (EGFR) pathway plays a major role in the pathogenesis of prostate cancer (PCa). However, therapies targeting EGFR have demonstrated limited effectiveness in PCa. A potential mechanism to overcome EGFR blockade in cancer cells is the autocrine activation of alter...
journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::We have carried out three rounds of screening tests using 239 synthetic tripeptides for the growth-inhibitory activity toward tumor cells and found that those having amino acid sequences of Pro-Thr-Trp or Glu-Arg-Pro effectively inhibit proliferation of transformed cells. These peptides could be the fundamental struct...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Thirty-three adult patients with anaplastic astrocytoma or glioblastoma multiforme, verified between 1990 and 1997, received postoperative intra-arterial ACNU chemotherapy and irradiation. Since our previous strategies to malignant gliomas had included intra-venous ACNU, the purpose of the present study is to evaluate...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:10.3892/ijo.13.3.537
更新日期:1998-09-01 00:00:00
abstract::Multiple drug resistance is reported to be a major obstacle in treatment of osteosarcoma (OS). Research has demonstrated that small subsets of cells called cancer stem cells (CSCs) are responsible for multiple drug resistance. CSCs are potential targets for reversing chemoresistance. In the present study, we compared ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3950
更新日期:2017-05-01 00:00:00
abstract::Double-stranded RNA-dependent protein kinase (PKR) is one of the players in the cellular antiviral responses and is involved in transcriptional stimulation through activation of NF-κB. Treatment of the human osteosarcoma cell line MG63 with the protein phosphatase inhibitor okadaic acid stimulated the expression and p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1911
更新日期:2013-06-01 00:00:00
abstract::The p16(INK4A) gene is frequently inactivated in nonsmall cell lung carcinoma (NSCLC) by either mutations, deletions or DNA methylations. To assess the biological significance of p16(INK4A) inactivation in the development of NSCLC, full-length p16(INK4A) cDNA was introduced into NSCLC cell lines, A549 and H322, in whi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.1.33
更新日期:1997-01-01 00:00:00
abstract::Hereditary nonpolyposis colorectal cancer (HNPCC) is a syndrome involving a predisposition to cancers of the colon, endometrium and several other extra-colonic sites, accounting for approximately 1-5% of all colorectal cancer cases. It is not easily recognized because of a lack of distinctive clinical markers, making ...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.12.4.947
更新日期:1998-04-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a malignant hematopoietic disorder distinguished by the presence of a BCR‑ABL1 fused oncogene with constitutive kinase activity. Targeted CML therapy by specific tyrosine kinase inhibitors (TKIs) leads to a marked improvement in the survival of the patients and their quality of life. ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5163
更新日期:2020-12-23 00:00:00
abstract::The expression of myeloid cell leukemia-1 (Mcl‑1), a member of the anti-apoptotic Bcl-2 protein family, has been associated with tumor progression and adverse patient outcome. The aims of current study were to evaluate whether Mcl-1 affects the survival or death of gastric cancer cells, and to investigate the prognost...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2890
更新日期:2015-05-01 00:00:00
abstract::Cancer-associated fibroblasts (CAFs) are key determinants in the malignant progression of cancer, supporting tumorigenesis and metastasis. CAFs also mediate epithelial to mesenchymal transition (EMT) in tumor cells and their achievement of stem cell traits. Curcumin has recently been found to possess anticancer activi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3202
更新日期:2015-12-01 00:00:00
abstract::Rhabdomyosarcoma (RMS) is a malignant tumour of skeletal muscle origin which includes two major histological subtypes: alveolar rhabdomyosarcoma (ARMS), the more aggressive, and embryonal rhabdomyosarcoma (ERMS). In order to establish whether the higher metastatic potential of ARMS cells may depend on differential exp...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::Cordycepin (3'-deoxyadenosine), a major bioactive compound of Cordyceps militaris, has many pharmacological actions, such as anti-inflammatory and anticancer activities. In this study, the relationship between inhibition of cell motility and anti-invasive activity by cordycepin in L...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1332
更新日期:2012-05-01 00:00:00
abstract::Currently, it is not entirely clear whether hypoxia-inducible factor-1α (HIF-1α) is involved in the regulation of COX-2 expression and epithelial-to-mesenchymal transition (EMT), and whether these events affect the prognosis of hepatocellular carcinoma (HCC) patients treated with transcatheter arterial chemoembolizati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3421
更新日期:2016-05-01 00:00:00
abstract::Human carcinogenesis is a multistep process in which epithelial cells progress through a series of premalignant phenotypes until an invasive cancer emerges. Extensive experimental observations in carcinogenesis have demonstrated this process can be divided into three general eras: initiation, promotion, and progressio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::The developmentally regulated PLAG1 proto-oncogene has been implicated in the development of various human tumor types, such as pleomorphic salivary gland adenomas, lipoblastomas, hepatoblastomas and AML. In previous studies, we generated two independent PLAG1 transgenic founder strains, PTMS1 and PTMS2, in which PLAG...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The intention of a surveillance program to monitor prostate cancer patients is to detect recurrence of disease as soon as possible and institute therapy at the time of recurrence in order to improve the chance of cure or extend survival. We analyzed the cost of four surveillance programs for prostate cancer. As PSA le...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.3.473
更新日期:1993-03-01 00:00:00
abstract::Squamous cell carcinoma antigen (SCCA) is overexpressed in many squamous cell cancers and SCCA‑derived peptide-specific CD8(+) cytotoxic T lymphocytes can display cytotoxicity against tumor cells. In the present study, we screened the SCCA amino acid sequence for potential HLA-A*0201-binding CD8(+) T‑cell epitopes usi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1834
更新日期:2013-04-01 00:00:00
abstract::The promising benefits of cancer gene therapy have been limited by the inability to deliver therapeutic genes homogeneously throughout the tumor mass and to control gene expression within the tumor cells. Transcriptional targeting, the use of DNA regulatory promoter sequences to localize transgene expression, has been...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-01 00:00:00
abstract::We review our recent experience with intensity-modulated radiation therapy (IMRT) and conventional three-dimensional radiation therapy (C3DRT) in advanced head and neck cancer. Sixty-nine patients with Stage IV head and neck cancer (and stage III base of tongue and hypopharynx) enrolled in a Phase II study of definiti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Tumor-bearing mice with two different locally growing malignant tumors (epithelial like, MCG 101; malignant melanoma, K1735-M2) were used to evaluate the putative role of prostaglandins for survival and local tumor growth in experimental cancer. Daily systemic injections of indomethacin (1 mu g/g bw) were used to bloc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.6.1405
更新日期:1995-12-01 00:00:00
abstract::Although many chemotherapies have been developed for melanomas, successful therapy would be aided by the identification of intrinsic mechanisms that are crucial for melanoma survival. Here, we used resveratrol, a phytoalexin, as an anti-melanoma reagent. Applying resveratrol to various human and murine melanoma cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2480
更新日期:2014-08-01 00:00:00