Pituitary adenylate cyclase-activating polypeptide (PACAP) stimulates the expression and the release of tissue plasminogen activator (tPA) in neuronal cells: involvement of tPA in the neuroprotective effect of PACAP.

abstract：:In this report we demonstrate that a defective herpes simplex virus type one (HSV-1) vector can express enzymatically active tyrosine hydroxylase in cultured striatal cells that are thereby converted into L-DOPA-producing cells. A human tyrosine hydroxylase cDNA (form II) was inserted into an HSV-1 vector (pHSVth) and...

journal_title：Journal of neurochemistry

authors： Geller AI,During MJ,Oh YJ,Freese A,O'Malley K

更新日期：1995-02-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) provides neuroprotection, but its neuroprotective mechanism has not been resolved. We investigated the neuroprotective mechanism of GDNF using primary culture of the rat mesencephalon. Bleomycin sulfate (BLM) and L-buthionine-[S,R]-sulfoximine (BSO) caused apoptosis i...

journal_title：Journal of neurochemistry

authors： Sawada H,Ibi M,Kihara T,Urushitani M,Nakanishi M,Akaike A,Shimohama S

更新日期：2000-03-01 00:00:00

abstract：:alpha-Conotoxins interact with nicotinic acetylcholine receptors (nAChRs) and acetylcholine-binding proteins (AChBPs) at the sites for agonists/competitive antagonists. alpha-Conotoxins blocking muscle-type or alpha7 nAChRs compete with alpha-bungarotoxin. However, alpha-conotoxin ImII, a close homolog of the alpha7 n...

journal_title：Journal of neurochemistry

authors： Kasheverov IE,Zhmak MN,Fish A,Rucktooa P,Khruschov AY,Osipov AV,Ziganshin RH,D'hoedt D,Bertrand D,Sixma TK,Smit AB,Tsetlin VI

更新日期：2009-11-01 00:00:00

abstract：:Neurofibrillary tangles, one of the major pathological hallmarks of Alzheimer-diseased brains, consist primarily of aggregated paired helical filaments (PHFs) of hyperphosphorylated tau protein. Tau from normal brain and especially from foetal brain is also phosphorylated on some of the sites phosphorylated in PHFs, m...

journal_title：Journal of neurochemistry

authors： Reynolds CH,Nebreda AR,Gibb GM,Utton MA,Anderton BH

更新日期：1997-07-01 00:00:00

abstract：:Neural stem cells (NSCs) play an essential role in both the developing embryonic nervous system through to adulthood where the capacity for self-renewal may be important for normal function of the CNS, such as in learning, memory and response to injury. There has been much excitement about the possibility of transplan...

journal_title：Journal of neurochemistry

authors： Tamm C,Duckworth J,Hermanson O,Ceccatelli S

更新日期：2006-04-01 00:00:00

abstract：:Fast-performance liquid chromatography was used to purify assembly-competent tubulin from porcine brain microtubule protein prepared by two cycles of assembly-disassembly. Microtubule protein (1-100 mg at 1.5-2.5 mg/ml) in buffer consisting of 0.1 M 2-(N-morpholino)ethanesulfonic acid, 0.5 mM MgCl2, 1 mM EGTA, 0.3 M K...

journal_title：Journal of neurochemistry

authors： Roychowdhury S,Gaskin F

更新日期：1986-05-01 00:00:00

abstract：:The survival and functional maintenance of vertebrate neurons depends on the availability of specific neurotrophic factors. We studied the influence of neurotrophic support on responses of dopaminergic neurons to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, a neurotoxin known to damage the nigrostriatal dopaminergic ...

journal_title：Journal of neurochemistry

authors： Hirata Y,Kiuchi K

更新日期：2007-09-01 00:00:00

abstract：:The lipid compositions of 10 different brain regions from patients affected by Alzheimer's disease/senile dementia of Alzheimer's type were analyzed. The total phospholipid amount decreased somewhat in nucleus caudatus and in white matter. The cortical areas that are morphologically affected by Alzheimer's disease, i....

journal_title：Journal of neurochemistry

authors： Söderberg M,Edlund C,Alafuzoff I,Kristensson K,Dallner G

更新日期：1992-11-01 00:00:00

abstract：:In this study, we examined the molecular and functional characterization of choline uptake into cultured rat cortical astrocytes. Choline uptake into astrocytes showed little dependence on extracellular Na+. Na+-independent choline uptake was saturable and mediated by a single transport system, with an apparent Michae...

journal_title：Journal of neurochemistry

authors： Inazu M,Takeda H,Matsumiya T

更新日期：2005-09-01 00:00:00

abstract：:We have investigated the isozymes of a phosphoinositide-specific phospholipase C (PLC) in bovine retina using several monoclonal antisera to PLCbeta1, gamma1, and delta1. Immunoblot analysis showed that all three isozymes were present in the retina. Immunocytochemical localization in frozen bovine retina sections show...

journal_title：Journal of neurochemistry

authors： Ghalayini AJ,Weber NR,Rundle DR,Koutz CA,Lambert D,Guo XX,Anderson RE

更新日期：1998-01-01 00:00:00

abstract：:Experimental allergic encephalomyelitis (EAE) is an autoimmune, animal model of multiple sclerosis (MS) in which demyelination and paralysis are evident. Quinolinic acid (QUIN) is a neurotoxin and endogenous N-methyl-D-aspartate receptor agonist formed from tryptophan. The role of neurotoxins in general and QUIN in pa...

journal_title：Journal of neurochemistry

authors： Flanagan EM,Erickson JB,Viveros OH,Chang SY,Reinhard JF Jr

更新日期：1995-03-01 00:00:00

abstract：:Stem cell-based approaches provide hope as a potential therapy for neurodegenerative diseases and stroke. One of the major scientific hurdles for stem cell therapy is the poor survival rate of the newly formed or transplanted neural stem cells. In this study, we found that low-dose treatment with the Heat shock protei...

journal_title：Journal of neurochemistry

authors： Wang G,Krishnamurthy K,Tangpisuthipongsa D

更新日期：2011-05-01 00:00:00

abstract：:The biggest risk factor for Alzheimer's disease is the process of ageing, but the mechanisms that lead to the manifestation of the disease remain to be elucidated. Why age triggers the disease is unclear but an emerging theme is the inability for a cell to efficiently maintain many key processes such as energy product...

journal_title：Journal of neurochemistry

authors： Roberts BR,Ryan TM,Bush AI,Masters CL,Duce JA

更新日期：2012-01-01 00:00:00

abstract：:The proposed structural protein of peripheral nerve myelin, P0, has been shown to have several covalent modifications. In addition to being glycosylated, sulfated, and acylated, P0 is phosphorylated, with the intracellular site of this latter addition being in question. By employing nerve injury models that exhibit di...

journal_title：Journal of neurochemistry

authors： Brunden KR,Poduslo JF

更新日期：1987-12-01 00:00:00

abstract：:Both adenosine and interleukin-6 (IL-6) have been implicated in the pathophysiology of, e.g., epileptic seizures, traumatic brain injury, and affective disorders. Stimulation of adenosine A2B receptors on astrocytes in vitro leads to the increased synthesis and secretion of IL-6. We investigated whether or not activat...

journal_title：Journal of neurochemistry

authors： Vazquez JF,Clement HW,Sommer O,Schulz E,van Calker D

更新日期：2008-05-01 00:00:00

abstract：:Metipranolol is a β-adrenergic receptor antagonist that is given orally for the treatment of hypertension and also applied topically to the cornea for treating glaucoma. It also inhibits nitrosative stress which has previously been shown to be the cause of cone photoreceptor death in retinitis pigmentosa. In this stud...

journal_title：Journal of neurochemistry

authors： Kanan Y,Khan M,Lorenc VE,Long D,Chadha R,Sciamanna J,Green K,Campochiaro PA

更新日期：2019-01-01 00:00:00

abstract：:We examined the effects of wild-type and mutant atlastin-1 on vesicle transport in the endoplasmic reticulum (ER)-Golgi interface and vesicle budding from ER-derived microsomes using the temperature-sensitive reporter vesicular stomatitis virus glycoprotein (VSV-G), and the ability of purified atlastin-1 to form tubul...

journal_title：Journal of neurochemistry

authors： Muriel MP,Dauphin A,Namekawa M,Gervais A,Brice A,Ruberg M

更新日期：2009-09-01 00:00:00

abstract：:We investigated by 13C NMR the turnover of the H3 deuterons of (2-13C) glutamate and (2-13C) glutamine in the brain of partially deuterated rats. Adult animals (150-200 g) fed ad libitum received 50% 2H2O or tap water 9 days before infusing (1-13C) glucose or (2-13C) acetate for 5, 10, 15, 30, 60, or 90 min. The brain...

journal_title：Journal of neurochemistry

authors： Cerdán S,Sierra A,Fonseca LL,Ballesteros P,Rodrigues TB

更新日期：2009-05-01 00:00:00

abstract：:The specific binding of [N-methyl-3H]flunitrazepam ([3H]FNZP) to a membrane fraction from the supraoesophageal ganglion of the locust (Schistocerca gregaria) has been measured. The ligand binds reversibly with a KD of 47 nM. The binding is Ca2+-dependent, a property not found for the equivalent binding site in vertebr...

journal_title：Journal of neurochemistry

authors： Robinson T,MacAllan D,Lunt G,Battersby M

更新日期：1986-12-01 00:00:00

abstract：:Previous studies showed that prolonged access to cocaine or heroin self-administration (long access, or LgA) produces an escalation in drug intake not observed with limited access to the drug (short access, or ShA). The present experiment employed in vivo microdialysis to test the role of alterations in drug pharmacok...

journal_title：Journal of neurochemistry

authors： Ahmed SH,Lin D,Koob GF,Parsons LH

更新日期：2003-07-01 00:00:00

abstract：:The blood-brain barrier (BBB) is important physiologically. Pathologically, BBB disruption has been implicated in a wide spectrum of neurological disorders including Parkinson's disease (PD). Recent studies indicate that caffeine is protective against PD, but by poorly understood mechanisms. Using a MPTP neurotoxin mo...

journal_title：Journal of neurochemistry

authors： Chen X,Lan X,Roche I,Liu R,Geiger JD

更新日期：2008-11-01 00:00:00

abstract：:The axonal outgrowth of cells of Neuro2a, a mouse neuroblastoma cell line, was suppressed on expression of the beta-galactoside alpha 1,2-fucosyltransferase (alpha 1,2-FT) gene. We recently cloned two types of rabbit alpha 1,2-FT, RFT-I and RFT-II. RFT-I exhibits comparable kinetic properties and structural homology w...

journal_title：Journal of neurochemistry

authors： Hitoshi S,Koijima N,Kusunoki S,Inokuchi J,Kanazawa I,Tsuji S

更新日期：1996-04-01 00:00:00

abstract：:Ischemic postconditioning is increasingly being investigated as a therapeutic approach for cerebral ischemia. However, the majority of studies are focused on the acute protection of neurons per se. Whether and how postconditioning affects multiple cells in the recovering neurovascular unit remains to be fully elucidat...

journal_title：Journal of neurochemistry

authors： Esposito E,Hayakawa K,Ahn BJ,Chan SJ,Xing C,Liang AC,Kim KW,Arai K,Lo EH

更新日期：2018-07-01 00:00:00

abstract：:Microglia are the main players of the brain immune response. They act as active sensors that rapidly respond to injurious insults by shifting into different activated states. Elevated levels of unconjugated bilirubin (UCB) induce cell death, immunostimulation and oxidative stress in both neurons and astrocytes. We rec...

journal_title：Journal of neurochemistry

authors： Silva SL,Osório C,Vaz AR,Barateiro A,Falcão AS,Silva RF,Brites D

更新日期：2011-05-01 00:00:00

abstract：:The presence of insulin receptors was investigated in human Y-79 retinoblastoma cells grown in suspension culture. The binding of [125I] insulin to these cells was time, temperature, and pH dependent, was competed for by insulin and proinsulin but not other peptides, and was inhibited by antibodies against the insulin...

journal_title：Journal of neurochemistry

authors： Saviolakis GA,Kyritsis AP,Chader GJ

更新日期：1986-07-01 00:00:00

abstract：:Experimental evidence suggests that magnesium plays a role in the pathophysiological sequelae of brain injury. The present study examined the variation of blood ionized and total magnesium, as well as potassium, sodium, and ionized calcium, after experimental fluid percussion brain injury in rats. Blood ionized magnes...

journal_title：Journal of neurochemistry

authors： Bareyre FM,Saatman KE,Helfaer MA,Sinson G,Weisser JD,Brown AL,McIntosh TK

更新日期：1999-07-01 00:00:00

abstract：:We have assessed the expression, molecular identification and functional role of Na+ channels (Na(v)) in carotid bodies (CB) obtained from normoxic and chronically hypoxic adult rats. Veratridine evoked release of catecholamines (CA) from an in vitro preparation of intact CBs obtained from normoxic animals, the respon...

journal_title：Journal of neurochemistry

authors： Caceres AI,Obeso A,Gonzalez C,Rocher A

更新日期：2007-07-01 00:00:00

abstract：:Using C8 reversed-phase HPLC in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis, we have fractionated proteins contained in human CSFs obtained from patients with schizophrenic disorders. When these proteins were electrophoretically blotted onto polyvinylidene difluoride membrane for direct ...

journal_title：Journal of neurochemistry

authors： Leone MG,Saso L,Del Vecchio A,Mo MY,Silvestrini B,Cheng CY

更新日期：1993-08-01 00:00:00

abstract：:Gs alpha, the GTP-binding protein that mediates receptor stimulation of adenylyl cyclase, exists in small (Gs alpha-S) and large (Gs alpha-L) molecular forms. Previous work has established that these Gs alpha isoforms are products of alternatively spliced Gs alpha pre-mRNA. The present work examined the splicing patte...

journal_title：Journal of neurochemistry

authors： Granneman JG,Bannon MJ

更新日期：1991-09-01 00:00:00

abstract：:2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a ubiquitous environmental pollutant that could induce significant toxic effects in the human nervous system. However, the underlying molecular mechanism has not been entirely elucidated. Reactive astrogliosis has implicated in various neurological diseases via the product...

journal_title：Journal of neurochemistry

authors： Zhang Y,Nie X,Tao T,Qian W,Jiang S,Jiang J,Li A,Guo A,Xu G,Wu Q

更新日期：2014-06-01 00:00:00