Molecular and functional characterization of an Na+-independent choline transporter in rat astrocytes.

abstract：:This study examined the respective influences of cannabinoid type-1 (CB1) receptors expressed either in forebrain GABAergic neurons, in cortical glutamatergic neurons, or in astrocytes on the turnover rates of the endocannabinoids N-arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG), and the non-cannabin...

journal_title：Journal of neurochemistry

authors： Belluomo I,Matias I,Pernègre C,Marsicano G,Chaouloff F

更新日期：2015-04-01 00:00:00

abstract：:Spiroxatrine has been reported to be a 5-HT1A serotonin receptor antagonist. Therefore [3H]spiroxatrine was synthesized and its 5-HT1A receptor binding properties in homogenates of rat hippocampal membranes were characterized with the expectation that it would be the first 5-HT1A antagonist radioligand. [3H]8-Hydroxyd...

journal_title：Journal of neurochemistry

authors： Herrick-Davis K,Titeler M

更新日期：1988-02-01 00:00:00

abstract：:Preincubation of murine neuroblastoma cells (clone N1E-115) with terbium chloride resulted in a significant potentiation of carbachol-mediated increase in cyclic GMP formation. This effect was accompanied by a shift of the peak response from 30 s to 120 s and a 6-fold decrease in carbachol concentration producing half...

journal_title：Journal of neurochemistry

authors： El-Fakahany EE,Pfenning M,Richelson E

更新日期：1984-03-01 00:00:00

abstract：:Neurodegenerative diseases such as Alzheimer's disease (AD) are characterized by an abnormal aggregation of misfolded beta-sheet rich proteins such as β-amyloid (Aβ). Various ubiquitously expressed molecular chaperones control the correct folding of cellular proteins and prevent the accumulation of harmful species. We...

journal_title：Journal of neurochemistry

authors： Hoshino A,Helwig M,Rezaei S,Berridge C,Eriksen JL,Lindberg I

更新日期：2014-02-01 00:00:00

abstract：:We have examined the metabolism of phosphate and sulfate groups modifying the P0 protein, the major protein of peripheral nervous system myelin, using an in vitro incubation system. Incorporation of [3H]leucine into the P0 peptide backbone decreased approximately 25-fold between 10 and 90 days of age, a finding reflec...

journal_title：Journal of neurochemistry

authors： Toews AD,Fischer HR,Goodrum JF,Windes S,Morell P

更新日期：1987-03-01 00:00:00

abstract：:The activities of the three major catecholamine-synthesizing enzymes were determined in brain tissue pellets dissected from 500-micrometers thick horizontal sections of rat lower brainstem. The rostrocaudal distributions of the three enzymatic activities were generally not parallel, suggesting differences in the respe...

journal_title：Journal of neurochemistry

authors： Chamba G,Denoroy L,Renaud B

更新日期：1982-08-01 00:00:00

abstract：:Prospective studies in humans and experimental investigations in animals have correlated elevated perinatal blood lead levels with enduring behavioural and cognitive perturbations. Although deficits in neuroplastic events necessary for long-term memory consolidation have been observed during the postnatal period, ther...

journal_title：Journal of neurochemistry

authors： Murphy KJ,Regan CM

更新日期：1999-05-01 00:00:00

abstract：:Changes in the activity of Na+,K+-ATPase and in the water, Na+, and K+ levels in the parietal cortex, hippocampus, and thalamus were investigated in rats 1, 3, 6, and 24 h following systemic kainic acid injection. An increase in Na+,K+-ATPase activity was observed in all three regions 3 h after the treatment, with a s...

journal_title：Journal of neurochemistry

authors： Sztriha L,Joó F,Dux L,Böti Z

更新日期：1987-07-01 00:00:00

abstract：:The loss of ATP, which is needed for ionic homeostasis, is an early event in the neurotoxicity of glutamate and beta-amyloid (A(beta)). We hypothesize that cells supplemented with the precursor creatine make more phosphocreatine (PCr) and create larger energy reserves with consequent neuroprotection against stressors....

journal_title：Journal of neurochemistry

authors： Brewer GJ,Wallimann TW

更新日期：2000-05-01 00:00:00

abstract：:Ammonia is responsible for cerebral edema associated with acute liver failure, but the role of the vasogenic mechanism has been a matter of dispute. Here, we tested the hypothesis that ammonia induces changes in blood-brain barrier (BBB) permeability by a mechanism coupled to oxidative/nitrosative stress (ONS) evoked ...

journal_title：Journal of neurochemistry

authors： Skowrońska M,Zielińska M,Wójcik-Stanaszek L,Ruszkiewicz J,Milatovic D,Aschner M,Albrecht J

更新日期：2012-04-01 00:00:00

abstract：:Adenylate cyclase in homogenates of Drosophila melanogaster is heterogeneous with respect to its affinity toward MgATP and its subcellular distribution. Km values for MgATP range, under similar assay conditions, from approximately 10(-5) M to approximately 10(-3) M, depending on the body region and on the subcellular ...

journal_title：Journal of neurochemistry

authors： Dudai Y,Zvi S

更新日期：1985-08-01 00:00:00

abstract：:Ten-minute incubation with 1 mM S-nitroso-L-cysteine (NO-CYS), a nitric oxide (NO) generator, induced a 3.5-fold increase in the basal dopamine (DA) efflux from rat striatal slices. Reduced hemoglobin (100 microM), a NO scavenger, blocked this response. Nomifensine (10 microM), an inhibitor of high-affinity DA transpo...

journal_title：Journal of neurochemistry

authors： Lonart G,Johnson KM

更新日期：1994-12-01 00:00:00

abstract：:Myelination, during both normal development and with respect to disorders of myelination, is commonly studied by morphological and/or biochemical techniques that assay as their end-points the extent of myelination. The rate of myelination is potentially a more useful parameter, but it is difficult and time-consuming t...

journal_title：Journal of neurochemistry

authors： Muse ED,Jurevics H,Toews AD,Matsushima GK,Morell P

更新日期：2001-01-01 00:00:00

abstract：:In the rat pilocarpine model, 1 h of status epilepticus caused significant inhibition of Mg(2+)/Ca(2+) ATPase-mediated Ca(2+) uptake in cortex endoplasmic reticulum (microsomes) isolated immediately after the status episode. The rat pilocarpine model is also an established model of acquired epilepsy. Several weeks aft...

journal_title：Journal of neurochemistry

authors： Parsons JT,Churn SB,DeLorenzo RJ

更新日期：2001-10-01 00:00:00

abstract：:Nuclei from the cerebral cortices of animals of different ages were separated into neuronal and neuroglial populations. Nuclei from cerebellar neurons were also studied. Using the enzyme micrococcal nuclease as a probe for chromatin structure, we found that the DNA from both neuronal preparations showed a decreased su...

journal_title：Journal of neurochemistry

authors： Berkowitz EM,Sanborn AC,Vaughan DW

更新日期：1983-08-01 00:00:00

abstract：:Spinocerebellar ataxia type-3, also known as Machado-Joseph Disease, is one of many inherited neurodegenerative disorders caused by polyglutamine-encoding CAG repeat expansions in otherwise unrelated disease genes. Polyglutamine disorders are characterized by disease protein misfolding and aggregation; often within th...

journal_title：Journal of neurochemistry

authors： Berke SJ,Schmied FA,Brunt ER,Ellerby LM,Paulson HL

更新日期：2004-05-01 00:00:00

abstract：:Increased cerebrovascular permeability is an important factor for the development of cerebral edema. To investigate the effect of hypoxia on the transport of blood-borne proteins to the brain, we used a cell culture model of the blood-brain barrier (BBB) consisting of a coculture of brain capillary endothelial cells a...

journal_title：Journal of neurochemistry

authors： Plateel M,Teissier E,Cecchelli R

更新日期：1997-02-01 00:00:00

abstract：:This study was undertaken to explore the metabolic fate of acetyl-L-carnitine in rat brain. To measure the flux of carbon atoms into anabolic processes occurring at regional levels, we have injected [1-(14)C]acetyl-L-carnitine into the lateral brain ventricle of conscious rats. After injection of [1-(14)C]acetyl-L-car...

journal_title：Journal of neurochemistry

authors： Ricciolini R,Scalibastri M,Kelleher JK,Carminati P,Calvani M,Arduini A

更新日期：1998-12-01 00:00:00

abstract：:Drosophila melanogaster homogenates incubated with tritiated 5-hydroxytryptophan, 5-hydroxytryptamine, or N-acetylserotonin have the ability of converting them into labelled indolamines, including melatonin. All these compounds were characterized by two-dimensional thin-layer chromatography by comparison with nonradio...

journal_title：Journal of neurochemistry

authors： Finocchiaro L,Callebert J,Launay JM,Jallon JM

更新日期：1988-02-01 00:00:00

abstract：:Substrate-induced endocytic trafficking of dopamine transporter (DAT) has been observed, but little is known about the regulation of DAT oligomerization by substrate. The present study investigates the effect on substrates on DAT oligomerization and explores a potential link with the presence of DAT at the cell surfac...

journal_title：Journal of neurochemistry

authors： Chen N,Reith ME

更新日期：2008-05-01 00:00:00

abstract：:The effects of short- and long-chain fatty acids on the cerebromicrovascular (Na+ + K+)-ATPase were investigated using specific [3H]ouabain binding to the enzyme. Specific binding increased linearly with total microvessel protein (37-110 micrograms) and was time-dependent with maximum binding obtained by 10 min. Arach...

journal_title：Journal of neurochemistry

authors： Caspers ML,Grammas P

更新日期：1988-04-01 00:00:00

abstract：:The long-term regulation of tyrosine hydroxylase (TH) by serotonin has been studied with cultures of newborn rat locus ceruleus explants. The presence of serotonin in the culture medium for a 24-h period was followed by an inhibition of TH activity in the explants. This effect lasted several days, with a maximal effec...

journal_title：Journal of neurochemistry

authors： Devau G,Multon MF,Pujol JF,Buda M

更新日期：1987-09-01 00:00:00

abstract：:The effect of tumor necrosis factor-alpha (TNF-alpha) on neuronal viability has been investigated in the SK-N-BE neuroblastoma cell line. These cells undergo differentiation upon chronic treatment with retinoic acid. Exposure of SK-N-BE cells to TNF-alpha produced a proliferative response in undifferentiated cells, wh...

journal_title：Journal of neurochemistry

authors： Condorelli F,Sortino MA,Stella AM,Canonico PL

更新日期：2000-09-01 00:00:00

abstract：:Dystonia musculorum (dt) mice suffer from a severe sensory neuropathy caused by mutations in the gene encoding the cytoskeletal cross-linker protein dystonin/bullous pemphigoid antigen 1 (Bpag1). Loss of function of dystonin/Bpag1 within neurons leads to a loss in the maintenance of cytoskeletal organization and to th...

journal_title：Journal of neurochemistry

authors： De Repentigny Y,Deschênes-Furry J,Jasmin BJ,Kothary R

更新日期：2003-08-01 00:00:00

abstract：:Temporal carbohydrate expression patterns at cell surfaces are thought to be of crucial regulatory significance during developmental processes. Hitherto, however, data on individual membrane proteins undergoing development-associated changes in glycosylation are sparsely. Here, we show that the two natriuretic peptide...

journal_title：Journal of neurochemistry

authors： Müller D,Hildebrand M,Lübberstedt J,Kuhn M,Middendorff R

更新日期：2010-11-01 00:00:00

abstract：:Carbamylcholine stimulated phospholipase C activity in astrocytes in primary culture from the mesencephalon but not from the striatum or cerebral cortex of the mouse embryo. An alpha 1-adrenergic-mediated response was observed in all astrocyte populations. 2-Chloroadenosine potentiated the alpha 1-adrenergic response ...

journal_title：Journal of neurochemistry

authors： el-Etr M,Cordier J,Torrens Y,Glowinski J,Prémont J

更新日期：1989-03-01 00:00:00

abstract：:We have used biologically active derivatives of beta-nerve growth factor (NGF), modified by biotinylation via carboxyl groups, to target the specific binding of liposomes to cultured rat and human tumor cells bearing NGF receptors. Liposomes, to be used for targeting, were prepared by conjugating streptavidin to phosp...

journal_title：Journal of neurochemistry

authors： Rosenberg MB,Breakefield XO,Hawrot E

更新日期：1987-03-01 00:00:00

abstract：:The role of protein kinase C (PKC) in modulating the release of the octapeptide cholecystokinin (CCK-8) was investigated in rat hippocampal nerve terminals (synaptosomes). The PKC-activating phorbol ester 4beta-phorbol 12,13-dibutyrate (beta-PDBu) dose dependently (5-5,000 nM) increased CCK-8 release in a strictly Ca2...

journal_title：Journal of neurochemistry

authors： Breukel AI,Wiegant VM,Lopes da Silva FH,Ghijsen WE

更新日期：1998-01-01 00:00:00

abstract：:Rat hippocampal glutamatergic terminals possess NMDA autoreceptors whose activation by low micromolar NMDA elicits glutamate exocytosis in the presence of physiological Mg(2+) (1.2 mM), the release of glutamate being significantly reduced when compared to that in Mg(2+)-free condition. Both glutamate and glycine were ...

journal_title：Journal of neurochemistry

authors： Musante V,Summa M,Cunha RA,Raiteri M,Pittaluga A

更新日期：2011-05-01 00:00:00

abstract：:Nanomolar β-amyloid peptide (Aβ) can induce neuronal loss in culture by activating microglia to phagocytose neurons. We report here that this neuronal loss is mediated by the bridging protein lactadherin/milk-fat globule epidermal growth factor-like factor 8 (MFG-E8), which is released by Aβ-activated microglia, binds...

journal_title：Journal of neurochemistry

authors： Neniskyte U,Brown GC

更新日期：2013-08-01 00:00:00