Targeting of liposomes to cells bearing nerve growth factor receptors mediated by biotinylated nerve growth factor.

Abstract:

:We have used biologically active derivatives of beta-nerve growth factor (NGF), modified by biotinylation via carboxyl groups, to target the specific binding of liposomes to cultured rat and human tumor cells bearing NGF receptors. Liposomes, to be used for targeting, were prepared by conjugating streptavidin to phospholipid amino groups on liposomes prepared by reverse-phase evaporation. Approximately 2,000 streptavidin molecules were incorporated per liposome. Addition of biotinylated NGF, but not of unmodified NGF, could mediate the subsequent binding of radiolabeled streptavidin-liposomes to rat pheochromocytoma PC12 cells in suspension at 4 degrees C. In contrast, incubation with biotinylated NGF did not mediate the binding of hemoglobin-conjugated liposomes. Under optimal incubation conditions, approximately 570 streptavidin-liposomes were specifically bound per cell. Biotinylated NGF was also used to obtain specific binding of streptavidin-liposomes containing encapsulated fluorescein isothiocyanate-labeled dextran to PC12 cells or human melanoma HS294 cells. When HS294 cells were incubated at 37 degrees C following targeted liposome binding at 4 degrees C, the cell-associated fluorescence appeared to become internalized, displaying a perinuclear pattern of fluorescence similar to that observed when lysosomes were stained with acridine orange. Trypsin treatment abolished cell-associated fluorescence when cells were held at 4 degrees C but did not alter the fluorescence pattern in cells following incubation at 37 degrees C. When liposomes containing carboxyfluorescein, a dye capable of diffusing out of acidic compartments, were targeted to HS294 cells, subsequent incubation at 37 degrees C resulted in diffuse cytoplasmic fluorescence, suggesting that internalized liposomes encounter lysosomal or prelysosomal organelles.

journal_name

J Neurochem

authors

Rosenberg MB,Breakefield XO,Hawrot E

doi

10.1111/j.1471-4159.1987.tb05597.x

subject

Has Abstract

pub_date

1987-03-01 00:00:00

pages

865-75

issue

3

eissn

0022-3042

issn

1471-4159

journal_volume

48

pub_type

杂志文章
  • A transient inhibition of mitochondrial ATP synthesis by nitric oxide synthase activation triggered apoptosis in primary cortical neurons.

    abstract::In order to investigate the relationship between nitric oxide-mediated regulation of mitochondrial function and excitotoxicity, the role of mitochondrial ATP synthesis and intracellular redox status on the mode of neuronal cell death was studied. Brief (5 min) glutamate (100 microM) receptor stimulation in primary cor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2001.00276.x

    authors: Almeida A,Bolaños JP

    更新日期:2001-04-01 00:00:00

  • Membrane-bound tetramer and trimer Aβ oligomeric species correlate with toxicity towards cultured neurons.

    abstract::Amyloid beta (Aβ) peptide is the major constituent of the extracellular amyloid plaques deposited in the brains of Alzheimer's disease patients and is central to the pathogenic pathway causing this disease. The identity of the neurotoxic Aβ species remains elusive. We previously reported that Aβ toxicity correlates st...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/jnc.13443

    authors: Jana MK,Cappai R,Pham CL,Ciccotosto GD

    更新日期:2016-02-01 00:00:00

  • Effects of choline and quiescence on Drosophila choline acetyltransferase expression and acetylcholine production by transduced rat fibroblasts.

    abstract::Rat-1 fibroblasts were transduced to express Drosophila choline acetyltransferase. The presence of an active enzyme in these cells (Rat-1/dChAT) was confirmed using various methods. Rat-1/dChAT fibroblasts released acetylcholine (ACh) into the culture medium. Moreover, intra- and extracellular levels of ACh could be i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1992.tb10942.x

    authors: Schinstine M,Rosenberg MB,Routledge-Ward C,Friedmann T,Gage FH

    更新日期:1992-06-01 00:00:00

  • The membrane receptors guanylyl cyclase-A and -B undergo distinctive changes in post-translational modification during brain development.

    abstract::Temporal carbohydrate expression patterns at cell surfaces are thought to be of crucial regulatory significance during developmental processes. Hitherto, however, data on individual membrane proteins undergoing development-associated changes in glycosylation are sparsely. Here, we show that the two natriuretic peptide...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.2010.06985.x

    authors: Müller D,Hildebrand M,Lübberstedt J,Kuhn M,Middendorff R

    更新日期:2010-11-01 00:00:00

  • Binding characteristics of a monoclonal beta-endorphin antibody recognizing the N-terminus of opioid peptides.

    abstract::The present paper describes the isolation and characterization of a clone of hybrid myelomas (3-E7) secreting a mouse monoclonal antibody to beta-endorphin. An examination of its specificity against a series of human beta-lipotropin fragments and other opioid peptides revealed that the N-terminus portion of beta-endor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1983.tb13560.x

    authors: Gramsch C,Meo T,Riethmüller G,Herz A

    更新日期:1983-05-01 00:00:00

  • Differences in the ligand binding properties of the short and long versions of the D2 dopamine receptor.

    abstract::The short and long forms of the D2 dopamine receptor have been expressed at similar levels in fibroblast Ltk- cells, and the long form of the receptor has also been expressed in Chinese hamster ovary cells. The ligand binding properties of the two forms of receptor expressed in different systems have been compared in ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1993.tb05863.x

    authors: Castro SW,Strange PG

    更新日期:1993-01-01 00:00:00

  • Activation of choline acetyltransferase by vasoactive intestinal peptide.

    abstract::Addition of vasoactive intestinal peptide (VIP) to brain homogenates increased the activity of choline acetyltransferase (ChAT) but not that of acetylcholinesterase or glucose-6-phosphate dehydrogenase. Activity of ChAT was increased in the anterior hypothalamus and in the dorsal and ventral hippocampus, but not in th...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1984.tb12721.x

    authors: Luine VN,Rostene W,Rhodes J,McEwen BS

    更新日期:1984-04-01 00:00:00

  • Enzymes of fatty acid beta-oxidation in developing brain.

    abstract::Developmental profiles were determined for the activities of eight enzymes involved in fatty acid beta-oxidation in rat brain. The enzymes studied were the palmitoyl-CoA, octanoyl-CoA, butyryl-CoA, glutaryl-CoA, and 3-hydroxyacyl-CoA dehydrogenases, the enoyl-CoA hydratase (crotonase), and the C4- and C10-thiolases. W...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1988.tb01044.x

    authors: Reichmann H,Maltese WA,DeVivo DC

    更新日期:1988-08-01 00:00:00

  • Localisation of microtubule-associated protein 1B phosphorylation sites recognised by monoclonal antibody SMI-31.

    abstract::MAP 1B is a microtubule-associated phosphoprotein that is expressed early in neurons and plays a role in axon growth. MAP 1B has two types of phospho-isoforms, one of which is developmentally down-regulated after neuronal maturation and one of which persists into adulthood. Because phosphorylation regulates MAP 1B bin...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.1997.69041417.x

    authors: Johnstone M,Goold RG,Bei D,Fischer I,Gordon-Weeks PR

    更新日期:1997-10-01 00:00:00

  • Amino acid release from cerebral cortex in experimental acute liver failure, studied by in vivo cerebral cortex microdialysis.

    abstract::Both increased gamma-aminobutyric acid (GABA)-ergic and decreased glutamatergic neurotransmission have been suggested relative to the pathophysiology of hepatic encephalopathy. This proposed disturbance in neurotransmitter balance, however, is based mainly on brain tissue analysis. Because the approach of whole tissue...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1992.tb09410.x

    authors: Bosman DK,Deutz NE,Maas MA,van Eijk HM,Smit JJ,de Haan JG,Chamuleau RA

    更新日期:1992-08-01 00:00:00

  • Release of gamma-amino[3H]butyric acid from cultured amacrine-like neurons mediated by different excitatory amino acid receptors.

    abstract::The release of preaccumulated gamma-amino[3H]butyric acid ([3H]GABA) from putative GABAergic amacrine cells was studied in neuronal monolayer cultures made from embryonic chick retina. Release was specifically stimulated by excitatory amino acid agonists. N-Methyl-D-aspartate (NMDA; EC50, 19.1 +/- 5.0 microM), kainic ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1991.tb02010.x

    authors: Hofmann HD,Möckel V

    更新日期:1991-03-01 00:00:00

  • Mass fragmentographic method for the determination of trace amounts of putative amino acid neurotransmitters and related compounds from brain perfusates collected in vivo.

    abstract::A mass fragmentographic method for the determination of trace amounts of amino acid neurotransmitter candidates from brain perfusates is described. The analytical procedure includes the measurements of glycine, beta-alanine, gamma-aminobutyric acid, proline, aspartic acid, and glutamic acid; alpha-alanine, leucine, an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1982.tb08650.x

    authors: Wolfensberger M,Amsler U

    更新日期:1982-02-01 00:00:00

  • The structural specificity of choline transport into cholinergic nerve terminals.

    abstract::The accumulation of choline, homocholine, and 4-hydroxybutyltrimethylammonium by rat brain synaptosomes was measured; the choline uptake mechanism transported homocholine but not hydroxybutyltrimethylammonium, which, in addition, did not block choline accumulation. In cats' superior cervical ganglia, preganglionic ner...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1981.tb01732.x

    authors: Collier B

    更新日期:1981-03-01 00:00:00

  • High-affinity binding of [3H]desipramine to rat brain: a presynaptic marker for noradrenergic uptake sites.

    abstract::High-affinity binding sites (apparent KD = 1.5 nM) for [3H]desipramine have been demonstrated and characterized in membranes prepared from rat brain. The binding of [3H]desipramine was found to be saturable, reversible, heat-sensitive, sodium-dependent, and regionally distributed among various regions of the brain. Hi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1982.tb05326.x

    authors: Rehavi M,Skolnick P,Brownstein MJ,Paul SM

    更新日期:1982-04-01 00:00:00

  • Ascorbate inhibits edema in brain slices.

    abstract::Ascorbate is an essential antioxidant in the CNS, localized predominantly in neuronal cytosol. Slices of mammalian brain rapidly lose ascorbate, however, when incubated in ascorbate-free media; brain slices also take up water and swell. Here we investigated water gain in coronal slices of rat forebrain incubated with ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2000.741263.x

    authors: Brahma B,Forman RE,Stewart EE,Nicholson C,Rice ME

    更新日期:2000-03-01 00:00:00

  • Nuclear calmodulin-binding proteins in rat neurons.

    abstract::By using a 125I-calmodulin overlay assay, three major high-affinity calmodulin-binding proteins, showing apparent molecular masses of 135, 60, and 50 kDa, have been detected in purified nuclear fractions isolated from rat neurons. It has been shown that after extraction of the nuclei with nucleases and high salt, all ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1993.tb03304.x

    authors: Pujol MJ,Bosser R,Vendrell M,Serratosa J,Bachs O

    更新日期:1993-04-01 00:00:00

  • The role of metallobiology and amyloid-β peptides in Alzheimer's disease.

    abstract::The biggest risk factor for Alzheimer's disease is the process of ageing, but the mechanisms that lead to the manifestation of the disease remain to be elucidated. Why age triggers the disease is unclear but an emerging theme is the inability for a cell to efficiently maintain many key processes such as energy product...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审

    doi:10.1111/j.1471-4159.2011.07500.x

    authors: Roberts BR,Ryan TM,Bush AI,Masters CL,Duce JA

    更新日期:2012-01-01 00:00:00

  • Fractalkine-induced activation of the phosphatidylinositol-3 kinase pathway attentuates microglial activation in vivo and in vitro.

    abstract::Several neurodegenerative disorders are associated with evidence of inflammation, one feature of which is increased activation of microglia, the most likely cellular source of inflammatory cytokines like interleukin-1beta. It is now recognized that interaction of microglia with other cells contributes to maintenance o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.2009.06253.x

    authors: Lyons A,Lynch AM,Downer EJ,Hanley R,O'Sullivan JB,Smith A,Lynch MA

    更新日期:2009-09-01 00:00:00

  • High serotonin and 5-hydroxyindoleacetic acid levels in limbic brain regions in a rat model of depression: normalization by chronic antidepressant treatment.

    abstract::Although alterations in serotonin levels and neurotransmission are associated with depressive disorders and effective antidepressant therapy, the exact cause of these disorders and the mode of action of antidepressant drugs are poorly understood. In a genetic rat model of depression [Flinders sensitive line (FSL) rats...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.1997.69062477.x

    authors: Zangen A,Overstreet DH,Yadid G

    更新日期:1997-12-01 00:00:00

  • Polarized actin bundles formed by human fascin-1: their sliding and disassembly on myosin II and myosin V in vitro.

    abstract::Fascin-1 is a putative bundling factor of actin filaments in the filopodia of neuronal growth cones. Here, we examined the structure of the actin bundle formed by human fascin-1 (actin/fascin bundle), and its mode of interaction with myosin in vitro. The distance between cross-linked filaments in the actin/bundle was ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2003.02058.x

    authors: Ishikawa R,Sakamoto T,Ando T,Higashi-Fujime S,Kohama K

    更新日期:2003-11-01 00:00:00

  • Mitochondrial permeability transition in the central nervous system: induction by calcium cycling-dependent and -independent pathways.

    abstract::Isolated rat CNS mitochondria and cultured cortical astrocytes were examined for behavior indicative of a mitochondrial permeability transition (mPT). Exposure of isolated CNS mitochondria to elevated calcium or phosphate or both produced loss of absorbance indicative of mitochondrial swelling. The absorbance decrease...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.1997.69020524.x

    authors: Kristal BS,Dubinsky JM

    更新日期:1997-08-01 00:00:00

  • Spatial learning activates neural cell adhesion molecule polysialylation in a corticohippocampal pathway within the medial temporal lobe.

    abstract::Transient and time-dependent modulations of neural cell adhesion molecule (NCAM) polysialylation in the dentate gyrus of the rodent hippocampus are a feature of spatial and nonspatial forms of learning. In the hippocampal formation, polysialic acid immunoreactivity was localized to granule-like cells and their mossy f...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.1997.68062538.x

    authors: O'Connell AW,Fox GB,Barry T,Murphy KJ,Fichera G,Foley AG,Kelly J,Regan CM

    更新日期:1997-06-01 00:00:00

  • Deoxycytidine transport and metabolism in choroid plexus.

    abstract::In vitro, the transport into and release of [3H]deoxycytidine from the isolated choroid plexus, the anatomical locus of the blood-cerebrospinal fluid barrier, were studied separately. By use of the ability of nitrobenzylthioinosine (NBTI) to inhibit deoxycytidine efflux from choroid plexus, the transport of 1 microM [...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1983.tb13593.x

    authors: Spector R,Huntoon S

    更新日期:1983-05-01 00:00:00

  • Muscarinic receptor-mediated phosphorylation of cyclic AMP response element binding protein in human neuroblastoma cells.

    abstract::This study describes the effect of signalling through muscarinic acetylcholine receptors on two transcription factors implicated in long-term synaptic plasticity and memory formation, EGR1 and the cyclic AMP response element binding protein (CREB). In SK-N-SH neuroblastoma cells, treatment with the cholinergic agonist...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2002.00992.x

    authors: Greenwood JM,Dragunow M

    更新日期:2002-07-01 00:00:00

  • Monovalent cations and striatal tyrosine hydroxylase.

    abstract::The kinetic properties of soluble tyrosine hydroxylase from rat striatum and the activation of the enzyme by the polyanion heparin were assessed as a function of the monovalent cations K+, Na+, tetramethylammonium (TMA+), and Tris. Substitution of K+ or Na+ for TMA+ or Tris can alter the kinetic properties of tyrosine...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1982.tb12541.x

    authors: Kuczenski R

    更新日期:1981-09-01 00:00:00

  • Circadian alteration in neurobiology during protracted opiate withdrawal in rats.

    abstract::Protracted opiate withdrawal can extend for months of disrupted hormonal circadian rhythms. We examined rodent behaviors and these circadian disturbances in hormone and peptide levels as well as brain clock gene expression during 60 days of protracted withdrawal. Our behavioral tests included open field, elevated plus...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.2010.06941.x

    authors: Li SX,Liu LJ,Jiang WG,Sun LL,Zhou SJ,Le Foll B,Zhang XY,Kosten TR,Lu L

    更新日期:2010-10-01 00:00:00

  • Cerebrin prohormone processing, distribution and action in Aplysia californica.

    abstract::The isolation, characterization, and bioactivity in the feeding circuitry of a novel neuropeptide in the Aplysia californica central nervous system are reported. The 17-residue amidated peptide, NGGTADALYNLPDLEKIamide, has been termed cerebrin due to its primary location in the cerebral ganglion. Liquid chromatographi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2001.00360.x

    authors: Li L,Floyd PD,Rubakhin SS,Romanova EV,Jing J,Alexeeva VY,Dembrow NC,Weiss KR,Vilim FS,Sweedler JV

    更新日期:2001-06-01 00:00:00

  • Incubation of tissue slices in the absence of Ca2+ and Mg2+ can cause nonspecific damage.

    abstract::Superfusion of striatal slices with a medium deficient in Ca2+ and Mg2+ caused a large and sustained increase in release of lactate dehydrogenase, a finding indicative of the disruption of plasma membranes. This was associated with an efflux of dopamine (DA) and the depletion of DA from the tissue. In addition, wherea...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.1991.tb11445.x

    authors: Lonart G,Zigmond MJ

    更新日期:1991-04-01 00:00:00

  • Down-regulation of muscarinic acetylcholine receptor M2 adversely affects the expression of Alzheimer's disease-relevant genes and proteins.

    abstract::Beta-amyloid peptides play a major role in the pathogenesis of Alzheimer's disease (AD). Therefore, preventing beta-amyloid formation by inhibition of the beta site amyloid precursor protein-cleaving enzyme (BACE) 1 is considered as a potential strategy to treat AD. Cholinergic mechanisms have been shown to control am...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1111/j.1471-4159.2005.03335.x

    authors: Zuchner T,Schliebs R,Perez-Polo JR

    更新日期:2005-10-01 00:00:00

  • The role of putative intragenic control elements in c-fos regulation by calcium and growth factor signalling pathways.

    abstract::Sequences in the transcribed region of the c-fos gene have been suggested to control c-fos induction following exposure of cells to mitogens or stimuli that increase intracellular calcium concentrations. Using a mutational analysis we show that putative regulatory elements present in the first intron of the human c-fo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章

    doi:10.1046/j.1471-4159.2001.00342.x

    authors: Lange CM,Bading H

    更新日期:2001-06-01 00:00:00