Targeting of liposomes to cells bearing nerve growth factor receptors mediated by biotinylated nerve growth factor.


:We have used biologically active derivatives of beta-nerve growth factor (NGF), modified by biotinylation via carboxyl groups, to target the specific binding of liposomes to cultured rat and human tumor cells bearing NGF receptors. Liposomes, to be used for targeting, were prepared by conjugating streptavidin to phospholipid amino groups on liposomes prepared by reverse-phase evaporation. Approximately 2,000 streptavidin molecules were incorporated per liposome. Addition of biotinylated NGF, but not of unmodified NGF, could mediate the subsequent binding of radiolabeled streptavidin-liposomes to rat pheochromocytoma PC12 cells in suspension at 4 degrees C. In contrast, incubation with biotinylated NGF did not mediate the binding of hemoglobin-conjugated liposomes. Under optimal incubation conditions, approximately 570 streptavidin-liposomes were specifically bound per cell. Biotinylated NGF was also used to obtain specific binding of streptavidin-liposomes containing encapsulated fluorescein isothiocyanate-labeled dextran to PC12 cells or human melanoma HS294 cells. When HS294 cells were incubated at 37 degrees C following targeted liposome binding at 4 degrees C, the cell-associated fluorescence appeared to become internalized, displaying a perinuclear pattern of fluorescence similar to that observed when lysosomes were stained with acridine orange. Trypsin treatment abolished cell-associated fluorescence when cells were held at 4 degrees C but did not alter the fluorescence pattern in cells following incubation at 37 degrees C. When liposomes containing carboxyfluorescein, a dye capable of diffusing out of acidic compartments, were targeted to HS294 cells, subsequent incubation at 37 degrees C resulted in diffuse cytoplasmic fluorescence, suggesting that internalized liposomes encounter lysosomal or prelysosomal organelles.


J Neurochem


Rosenberg MB,Breakefield XO,Hawrot E




Has Abstract


1987-03-01 00:00:00












  • Functional effects of tau gene mutations deltaN296 and N296H.

    abstract::Mutations in the tau gene cause frontotemporal dementia and parkinsonism linked to chromosome-17 (FTDP-17). Functionally, about half of the known mutations increase the alternative mRNA splicing of exon 10 of the tau gene, resulting in the overproduction of tau isoforms with four microtubule-binding repeats. The other...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yoshida H,Crowther RA,Goedert M

    更新日期:2002-02-01 00:00:00

  • Systemically administered neuregulin-1β1 rescues nigral dopaminergic neurons via the ErbB4 receptor tyrosine kinase in MPTP mouse models of Parkinson's disease.

    abstract::Previously, we demonstrated that systemically injected extracellular domain of neuregulin-1β1 (Nrg1β1), a nerve growth and differentiation factor, passes the blood-brain barrier and rescues dopaminergic neurons of substantia nigra in the 6-hydroxydopamine-mouse model of Parkinson's disease (PD). Here, we studied the e...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Depboylu C,Rösler TW,de Andrade A,Oertel WH,Höglinger GU

    更新日期:2015-05-01 00:00:00

  • Brain mitochondria catalyze the oxidation of 7-(2-aminoethyl)-3,4-dihydro-5-hydroxy-2H-1,4-benzothiazine-3-carboxyli c acid (DHBT-1) to intermediates that irreversibly inhibit complex I and scavenge glutathione: potential relevance to the pathogenesis of

    abstract::We have proposed that a very early step in the pathogenesis of idiopathic Parkinson's disease is the elevated translocation of L-cysteine into neuromelanin-pigmented dopaminergic neurons in the substantia nigra. This influx of L-cysteine was proposed to divert the normal neuromelanin pathway by scavenging dopamine-o-q...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Li H,Shen XM,Dryhurst G

    更新日期:1998-11-01 00:00:00

  • The Reck tumor suppressor protein alleviates tissue damage and promotes functional recovery after transient cerebral ischemia in mice.

    abstract::The extracellular matrix (ECM) is important for both structural integrity and functions of the brain. Matrix metalloproteinases (MMPs) play major roles in ECM-remodeling under both physiological and pathological conditions. Reversion-inducing cysteine-rich protein with Kazal motifs (Reck) is a membrane-anchored MMP-re...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang H,Imamura Y,Ishibashi R,Chandana EP,Yamamoto M,Noda M

    更新日期:2010-10-01 00:00:00

  • Characteristics of phorbol ester- and agonist-induced down-regulation of astrocyte receptors coupled to inositol phospholipid metabolism.

    abstract::We have examined some of the characteristics of phorbol ester- and agonist-induced down-regulation of astrocyte receptors coupled to phosphoinositide metabolism. Our results show that preincubation of [3H]inositol-labelled astrocyte cultures with phorbol 12-myristate 13-acetate (PMA) resulted in a time- (t 1/2, 1-2 mi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pearce B,Morrow C,Murphy S

    更新日期:1988-03-01 00:00:00

  • Elevated intracellular Na+ concentrations in developing spinal neurons.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lindsly C,Gonzalez-Islas C,Wenner P

    更新日期:2017-03-01 00:00:00

  • Dysbindin-1 modifies signaling and cellular localization of recombinant, human D₃ and D₂ receptors.

    abstract::Dystrobrevin binding protein-1 (dysbindin-1), a candidate gene for schizophrenia, modulates cognition, synaptic plasticity and frontocortical circuitry and interacts with glutamatergic and dopaminergic transmission. Loss of dysbindin-1 modifies cellular trafficking of dopamine (DA) D2 receptors to increase cell surfac...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schmieg N,Rocchi C,Romeo S,Maggio R,Millan MJ,Mannoury la Cour C

    更新日期:2016-03-01 00:00:00

  • Production of platelet-activating factor from rat cerebellar granule cells in culture.

    abstract::Platelet-activating factor (PAF) is a potent lipid mediator implicated in various pathological conditions, including CNS neuronal injury. However, the production of PAF by mammalian CNS neurons has not as yet been demonstrated. In the present study, we demonstrate that PAF is produced by cultured rat cerebellar granul...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yue TL,Lysko PG,Feuerstein G

    更新日期:1990-05-01 00:00:00

  • Changes in NMDA receptor subunit expression in response to contusive spinal cord injury.

    abstract::Differential assembly of N-methyl-D-aspartate (NMDA) receptor subunits determines their functional characteristics. Using in situ hybridization, we found a selective increase of the subunits NR1 and NR2A mRNA at 24 h in ventral motor neurons (VMN) caudal to a standardized spinal cord contusion injury (SCI). Other neur...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Grossman SD,Wolfe BB,Yasuda RP,Wrathall JR

    更新日期:2000-07-01 00:00:00

  • Hypoxia-inducible factor-1α mediates up-regulation of neprilysin by histone deacetylase-1 under hypoxia condition in neuroblastoma cells.

    abstract::Hypoxia-inducible factor (HIF)-1 is the key transcriptional activator mediating both adaptive and pathological responses to hypoxia. The purpose of this study was to find the role of HIF-1 in regulating neprilysin (NEP) at the early stage of hypoxia and explore the underlying mechanism. In this study, we demonstrated ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang H,Sun M,Yang H,Tian X,Tong Y,Zhou T,Zhang T,Fu Y,Guo X,Fan D,Yu A,Fan M,Wu X,Xiao W,Chui D

    更新日期:2014-10-01 00:00:00

  • Induction of cytosolic progestin binding sites by catecholestrogens in rat pituitary gland and uterus: different potencies of 2- and 4-hydroxyestradiol.

    abstract::The ability of catecholestrogens to induce cytosolic progestin binding sites in the hypothalamus, pituitary gland, and uterus of ovariectomised-adrenalectomised rats was demonstrated by the increase in high-affinity [3H]promegestone binding sites (KD 1.39, 0.50, and 0.54 nM, respectively) following a single subcutaneo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kirchhoff J,Reinhardt W,Grünke W,Ghraf R,Ball P,Knuppen R

    更新日期:1983-02-01 00:00:00

  • Islet-activating protein inhibits the beta-adrenergic receptor facilitation elicited by gamma-aminobutyric acidB receptors.

    abstract::gamma-Aminobutyric acidB (GABAB) receptor recognition sites that inhibit cyclic AMP formation, open potassium channels, and close calcium channels are coupled to these effector systems by guanine nucleotide binding proteins (G proteins). These G proteins are ADP-ribosylated by islet-activating protein (IAP), also know...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wojcik WJ,Ulivi M,Paez X,Costa E

    更新日期:1989-09-01 00:00:00

  • Recovery from edema and of protein synthesis differs between the cortex and caudate following transient focal cerebral ischemia in rats.

    abstract::Postischemic recovery from brain edema and of protein synthesis was examined following 1 h of middle cerebral artery (MCA) occlusion in rats. Recovery from brain edema and of protein synthesis showed a good correlation until 7 days after reperfusion in each area (cerebral cortex or lateral caudate) in the occluded MCA...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Abe K,Araki T,Kogure K

    更新日期:1988-11-01 00:00:00

  • Ontogeny of receptor binding sites for [3H]glutamic acid and [3H]kainic acid in the rat cerebellum.

    abstract::The development of the specific binding sites for L-[3H]glutamic acid (KD = 370 nM) and for [3H]kainic acid (KD = 39 nM) was studied in the rat cerebellum. Specific binding at both sites remains low during the first week after birth but increases markedly during the second and third weeks after birth, when glutamaterg...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Slevin JT,Coyle JT

    更新日期:1981-08-01 00:00:00

  • Integrins stimulate phosphorylation of neurofilament NF-M subunit KSP repeats through activation of extracellular regulated-kinases (Erk1/Erk2) in cultured motoneurons and transfected NIH 3T3 cells.

    abstract::Integrin-mediated interactions of cells with components of the extracellular matrix (ECM) regulate cell survival, cell proliferation, cell differentiation and cell migration through activation of multiple intracellular signal transduction pathways. In this study, we have demonstrated that integrin-matrix interactions ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Li BS,Daniels MP,Pant HC

    更新日期:2001-02-01 00:00:00

  • In vivo production and release of acetylcholine from primary fibroblasts genetically modified to express choline acetyltransferase.

    abstract::Primary rat fibroblasts genetically modified to express Drosophila choline acetyltransferase (dChAT) synthesize and release acetylcholine (ACh) in vitro. The ACh produced from the transduced fibroblasts was found to be enhanced by increasing amounts of choline chloride in the culture media. These dChAT-expressing cell...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Fisher LJ,Schinstine M,Salvaterra P,Dekker AJ,Thal L,Gage FH

    更新日期:1993-10-01 00:00:00

  • Induction of nitric oxide synthase inhibits gap junction permeability in cultured rat astrocytes.

    abstract::Nitric oxide (.NO) synthase (NOS) was induced in cultured rat astrocytes by incubation with lipopolysaccharide (LPS) for 18 h and gap junction permeability was assessed by the scrape-loading/Lucifer yellow transfer technique. Induction of NOS was confirmed by determining either the NG-methyl-L-arginine (NMMA)-inhibita...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bolaños JP,Medina JM

    更新日期:1996-05-01 00:00:00

  • Blockade of neurotensin receptor by SR 48692 potentiates the facilitatory effect of haloperidol on the evoked in vivo dopamine release in the rat nucleus accumbens.

    abstract::The present experiments assessed the effects of SR 48692, a selective nonpeptide antagonist of neurotensin receptors, on mesolimbic dopaminergic neurotransmission. Dopamine release evoked by the electrical stimulation of the median forebrain bundle (20 Hz, 10 s) was measured in the nucleus accumbens of urethane-anesth...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Brun P,Steinberg R,Le Fur G,Soubrié P

    更新日期:1995-05-01 00:00:00

  • Soluble Aβ oligomer production and toxicity.

    abstract::For nearly 100 years following the first description of this neurological disorder by Dr Alois Alzheimer, amyloid plaques and neurofibrillary tangles have been hypothesized to cause neuronal loss. With evidence that the extent of insoluble, deposited amyloid poorly correlated with cognitive impairment, research effort...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Larson ME,Lesné SE

    更新日期:2012-01-01 00:00:00

  • The effects of asphyxia on 5-hydroxytryptamine metabolism in the immature rat brain.

    abstract::Five-day-old rats subjected to prolonged asphyxia at 37 degrees C show a slower rate of synthesis of 5-HT as measured by the accumulation of the amine after inhibition of monoamine oxidase. During recovery from asphyxia 5-HT synthesis is markedly stimulated when measured over a 50 or 90 min period. No change in 5-HT s...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tricklebank MD,Pickard FJ,Dobbing J

    更新日期:1976-11-01 00:00:00

  • HDL mimetic peptides affect apolipoprotein E metabolism: equal supplement or functional enhancer?: An Editorial for 'High-density lipoprotein mimetic peptide 4F mitigates amyloid-β-induced inhibition of apolipoprotein E secretion and lipidation in primary

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    journal_title:Journal of neurochemistry

    pub_type: 评论,社论


    authors: Wang W,Zhu X

    更新日期:2018-12-01 00:00:00

  • Steroid hormones block amyloid fibril-induced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) formazan exocytosis: relationship to neurotoxicity.

    abstract::Perhaps the most reproducible early event induced by the interaction of amyloid beta peptide (A beta) with the cell is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. We recently demonstrated that cytotoxic amyloid peptides such as A beta and human amylin inhibi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Liu Y,Schubert D

    更新日期:1998-12-01 00:00:00

  • Three unique 5' untranslated regions are spliced to common coding exons of high- and low-molecular-weight microtubule-associated protein-2.

    abstract::Three unique 5' untranslated regions (UTRs) have been characterized for human microtubule-associated protein-2 (MAP-2) transcripts. All three UTRs shared a common 171-bp sequence adjacent to the MAP-2 coding region and then diverged upstream. The size of the unique upstream sequence was 281, 146, or 104 bp. PCR of gen...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kalcheva N,Shafit-Zagardo B

    更新日期:1995-10-01 00:00:00

  • Cholinergic modulation of amyloid precursor protein processing with emphasis on M1 muscarinic receptor: perspectives and challenges in treatment of Alzheimer's disease.

    abstract::The prescribed drugs for treatment of cognitive deficits in Alzheimer's disease (AD) patients are regarded as symptomatic drugs. Effective disease modifying therapies are not yet prescribed in AD patients. Three major hallmarks of AD (e.g. cholinergic hypofunction, Aβ and tau neuropathologies) are closely linked raisi...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Fisher A

    更新日期:2012-01-01 00:00:00

  • Differential release of endogenous 5-hydroxytryptamine, substance P, and neurokinin A from rat ventral spinal cord in response to electrical stimulation.

    abstract::The release of endogenous 5-hydroxytryptamine (5-HT), substance P (SP), and neurokinin A (NKA) from superfused tissue slices of rat ventral lumbar spinal cord, where SP and NKA coexist with 5-HT in terminals of descending bulbospinal neurons, was investigated. Electrical field stimulation was performed using square-wa...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Franck J,Brodin E,Fried G

    更新日期:1993-08-01 00:00:00

  • Phenytoin dephosphorylates the catalytic subunit of the (Na+,K+)-ATPase in C57/BL mice.

    abstract::The effects of phenytoin, a potent antiepileptic drug, on the active transport of cations within membranes remain controversial. To assess the direct effects of phenytoin on the Na+,K+ pump, we studied the drug's influence on the phosphorylation of partially purified (Na+,K+)-ATPase from mouse brain. (Na+,K+)-ATPase s...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Guillaume D,Grisar T,Delgado-Escueta AV

    更新日期:1986-09-01 00:00:00

  • The metabolism of C-glucose by neurons and astrocytes in brain subregions following focal cerebral ischemia in rats.

    abstract::To provide insights into the effects of temporary focal ischemia on the function of neurons and astrocytes in vivo, we measured the incorporation of radiolabel from [U-14C]glucose into both glutamate and glutamine in brain subregions at 1 h of reperfusion following occlusion of the middle cerebral artery for 2 or 3 h....

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Thoren AE,Helps SC,Nilsson M,Sims NR

    更新日期:2006-05-01 00:00:00

  • Functional analysis and tissue-specific expression of Drosophila Na+,K+-ATPase subunits.

    abstract::We have previously purified and characterized a nervous system-specific glycoprotein antigen from adult Drosophila heads, designated Nervana [nerve antigen (NRV)] and identified two separate genes coding for three different proteins. All three proteins share homology with the beta subunits of Na+,K+-ATPase from variou...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sun B,Wang W,Salvaterra PM

    更新日期:1998-07-01 00:00:00

  • Glucocorticoid regulation of spermidine acetylation in the rat brain.

    abstract::The effect of glucocorticoids on polyamine metabolism has been elucidated further by measuring putrescine, spermidine, and spermine levels as well as ornithine decarboxylase, S-adenosylmethionine decarboxylase, and N1-acetylspermidine transferase activities in the hippocampus, cerebellar cortex, vermis, and deep nucle...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ientile R,De Luca G,Di Giorgio RM,Macaione S

    更新日期:1988-09-01 00:00:00

  • Zinc ions stabilise the association of basic protein with brain myelin membranes.

    abstract::Myelin basic protein (MBP) dissociated from brain myelin membranes when they were incubated (37 degrees C; pH 7.4) at physiological ionic strength. Zinc ions inhibited, and calcium promoted, this process. Protease activity in the membrane preparations cleaved the dissociated MBP into both small (less than 4 kilodalton...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Earl C,Chantry A,Mohammad N,Glynn P

    更新日期:1988-09-01 00:00:00