Targeting of liposomes to cells bearing nerve growth factor receptors mediated by biotinylated nerve growth factor.


:We have used biologically active derivatives of beta-nerve growth factor (NGF), modified by biotinylation via carboxyl groups, to target the specific binding of liposomes to cultured rat and human tumor cells bearing NGF receptors. Liposomes, to be used for targeting, were prepared by conjugating streptavidin to phospholipid amino groups on liposomes prepared by reverse-phase evaporation. Approximately 2,000 streptavidin molecules were incorporated per liposome. Addition of biotinylated NGF, but not of unmodified NGF, could mediate the subsequent binding of radiolabeled streptavidin-liposomes to rat pheochromocytoma PC12 cells in suspension at 4 degrees C. In contrast, incubation with biotinylated NGF did not mediate the binding of hemoglobin-conjugated liposomes. Under optimal incubation conditions, approximately 570 streptavidin-liposomes were specifically bound per cell. Biotinylated NGF was also used to obtain specific binding of streptavidin-liposomes containing encapsulated fluorescein isothiocyanate-labeled dextran to PC12 cells or human melanoma HS294 cells. When HS294 cells were incubated at 37 degrees C following targeted liposome binding at 4 degrees C, the cell-associated fluorescence appeared to become internalized, displaying a perinuclear pattern of fluorescence similar to that observed when lysosomes were stained with acridine orange. Trypsin treatment abolished cell-associated fluorescence when cells were held at 4 degrees C but did not alter the fluorescence pattern in cells following incubation at 37 degrees C. When liposomes containing carboxyfluorescein, a dye capable of diffusing out of acidic compartments, were targeted to HS294 cells, subsequent incubation at 37 degrees C resulted in diffuse cytoplasmic fluorescence, suggesting that internalized liposomes encounter lysosomal or prelysosomal organelles.


J Neurochem


Rosenberg MB,Breakefield XO,Hawrot E




Has Abstract


1987-03-01 00:00:00












  • A transient inhibition of mitochondrial ATP synthesis by nitric oxide synthase activation triggered apoptosis in primary cortical neurons.

    abstract::In order to investigate the relationship between nitric oxide-mediated regulation of mitochondrial function and excitotoxicity, the role of mitochondrial ATP synthesis and intracellular redox status on the mode of neuronal cell death was studied. Brief (5 min) glutamate (100 microM) receptor stimulation in primary cor...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Almeida A,Bolaños JP

    更新日期:2001-04-01 00:00:00

  • Membrane-bound tetramer and trimer Aβ oligomeric species correlate with toxicity towards cultured neurons.

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    pub_type: 杂志文章


    authors: Jana MK,Cappai R,Pham CL,Ciccotosto GD

    更新日期:2016-02-01 00:00:00

  • Effects of choline and quiescence on Drosophila choline acetyltransferase expression and acetylcholine production by transduced rat fibroblasts.

    abstract::Rat-1 fibroblasts were transduced to express Drosophila choline acetyltransferase. The presence of an active enzyme in these cells (Rat-1/dChAT) was confirmed using various methods. Rat-1/dChAT fibroblasts released acetylcholine (ACh) into the culture medium. Moreover, intra- and extracellular levels of ACh could be i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schinstine M,Rosenberg MB,Routledge-Ward C,Friedmann T,Gage FH

    更新日期:1992-06-01 00:00:00

  • The membrane receptors guanylyl cyclase-A and -B undergo distinctive changes in post-translational modification during brain development.

    abstract::Temporal carbohydrate expression patterns at cell surfaces are thought to be of crucial regulatory significance during developmental processes. Hitherto, however, data on individual membrane proteins undergoing development-associated changes in glycosylation are sparsely. Here, we show that the two natriuretic peptide...

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    authors: Müller D,Hildebrand M,Lübberstedt J,Kuhn M,Middendorff R

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Gramsch C,Meo T,Riethmüller G,Herz A

    更新日期:1983-05-01 00:00:00

  • Differences in the ligand binding properties of the short and long versions of the D2 dopamine receptor.

    abstract::The short and long forms of the D2 dopamine receptor have been expressed at similar levels in fibroblast Ltk- cells, and the long form of the receptor has also been expressed in Chinese hamster ovary cells. The ligand binding properties of the two forms of receptor expressed in different systems have been compared in ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Castro SW,Strange PG

    更新日期:1993-01-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Luine VN,Rostene W,Rhodes J,McEwen BS

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    journal_title:Journal of neurochemistry

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    authors: Reichmann H,Maltese WA,DeVivo DC

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    authors: Johnstone M,Goold RG,Bei D,Fischer I,Gordon-Weeks PR

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  • Amino acid release from cerebral cortex in experimental acute liver failure, studied by in vivo cerebral cortex microdialysis.

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    authors: Bosman DK,Deutz NE,Maas MA,van Eijk HM,Smit JJ,de Haan JG,Chamuleau RA

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hofmann HD,Möckel V

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wolfensberger M,Amsler U

    更新日期:1982-02-01 00:00:00

  • The structural specificity of choline transport into cholinergic nerve terminals.

    abstract::The accumulation of choline, homocholine, and 4-hydroxybutyltrimethylammonium by rat brain synaptosomes was measured; the choline uptake mechanism transported homocholine but not hydroxybutyltrimethylammonium, which, in addition, did not block choline accumulation. In cats' superior cervical ganglia, preganglionic ner...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Collier B

    更新日期:1981-03-01 00:00:00

  • High-affinity binding of [3H]desipramine to rat brain: a presynaptic marker for noradrenergic uptake sites.

    abstract::High-affinity binding sites (apparent KD = 1.5 nM) for [3H]desipramine have been demonstrated and characterized in membranes prepared from rat brain. The binding of [3H]desipramine was found to be saturable, reversible, heat-sensitive, sodium-dependent, and regionally distributed among various regions of the brain. Hi...

    journal_title:Journal of neurochemistry

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    authors: Rehavi M,Skolnick P,Brownstein MJ,Paul SM

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  • Ascorbate inhibits edema in brain slices.

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    authors: Brahma B,Forman RE,Stewart EE,Nicholson C,Rice ME

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  • Nuclear calmodulin-binding proteins in rat neurons.

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    journal_title:Journal of neurochemistry

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    authors: Pujol MJ,Bosser R,Vendrell M,Serratosa J,Bachs O

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  • The role of metallobiology and amyloid-β peptides in Alzheimer's disease.

    abstract::The biggest risk factor for Alzheimer's disease is the process of ageing, but the mechanisms that lead to the manifestation of the disease remain to be elucidated. Why age triggers the disease is unclear but an emerging theme is the inability for a cell to efficiently maintain many key processes such as energy product...

    journal_title:Journal of neurochemistry

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    authors: Roberts BR,Ryan TM,Bush AI,Masters CL,Duce JA

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    authors: Lyons A,Lynch AM,Downer EJ,Hanley R,O'Sullivan JB,Smith A,Lynch MA

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  • High serotonin and 5-hydroxyindoleacetic acid levels in limbic brain regions in a rat model of depression: normalization by chronic antidepressant treatment.

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    authors: Zangen A,Overstreet DH,Yadid G

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  • Polarized actin bundles formed by human fascin-1: their sliding and disassembly on myosin II and myosin V in vitro.

    abstract::Fascin-1 is a putative bundling factor of actin filaments in the filopodia of neuronal growth cones. Here, we examined the structure of the actin bundle formed by human fascin-1 (actin/fascin bundle), and its mode of interaction with myosin in vitro. The distance between cross-linked filaments in the actin/bundle was ...

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    authors: Ishikawa R,Sakamoto T,Ando T,Higashi-Fujime S,Kohama K

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  • Mitochondrial permeability transition in the central nervous system: induction by calcium cycling-dependent and -independent pathways.

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    authors: Kristal BS,Dubinsky JM

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  • Spatial learning activates neural cell adhesion molecule polysialylation in a corticohippocampal pathway within the medial temporal lobe.

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  • Deoxycytidine transport and metabolism in choroid plexus.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Spector R,Huntoon S

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  • Muscarinic receptor-mediated phosphorylation of cyclic AMP response element binding protein in human neuroblastoma cells.

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    authors: Greenwood JM,Dragunow M

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kuczenski R

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    pub_type: 杂志文章


    authors: Li SX,Liu LJ,Jiang WG,Sun LL,Zhou SJ,Le Foll B,Zhang XY,Kosten TR,Lu L

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Zuchner T,Schliebs R,Perez-Polo JR

    更新日期:2005-10-01 00:00:00

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lange CM,Bading H

    更新日期:2001-06-01 00:00:00