Antibody recognition of calcium-binding proteins depends on their calcium-binding status.


:Previous studies have revealed changes in immunohistochemical stains for calcium-binding proteins after manipulations that influence intracellular calcium. Cases have been revealed in which these changes in immunoreactivity were not correlated with changes in protein amounts. The present experiments examined whether these effects might be explained by changes in antiserum recognition due to calcium-induced changes in protein conformation. Calretinin, calbindin D28k, and parvalbumin incubated in high calcium were recognized by antisera better than when they were incubated in low calcium. Using a calbindin D28k antibody, it was shown that this effect occurs within physiological calcium concentrations. Formalin fixation of the proteins in the presence of calcium resulted in greater antibody recognition than did fixation of proteins in calcium-free states. The calretinin antiserum appeared to recognize a portion of the molecule previously shown to undergo calcium-dependent conformational changes. A calcium-insensitive antiserum was made to a different fragment of calretinin. These results indicate that some antibodies to calcium-binding proteins preferentially recognize particular calcium-induced protein conformations. Given the potential for wide fluctuations in neuronal calcium, the present results indicate that quantitative estimates of intracellular calcium-binding proteins obtained from immunohistochemical studies of neurons must be interpreted with caution.


J Neurochem


Winsky L,Kuźnicki J




Has Abstract


1996-02-01 00:00:00












  • Characterization of the exocytotic release of glutamate from guinea-pig cerebral cortical synaptosomes.

    abstract::A continuous enzyme-linked fluorometric assay was used for determining the characteristics for glutamate exocytosis from guinea-pig cerebrocortical synaptosomes. Ca2+-dependent release can be induced not only by K+, but also by the Na+ channel activator veratridine and the Ca2+ ionophore ionomycin. K+-induced release ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sanchez-Prieto J,Sihra TS,Nicholls DG

    更新日期:1987-07-01 00:00:00

  • Regulation and function of somatostatin receptors.

    abstract::This review summarizes the latest advances that have been made to elucidate the somatostatinergic system in respect to somatostatin receptor evolution, the development of receptor agonists/antagonists, receptor regulation, signal transduction, effects on cell proliferation, receptor-receptor or receptor-protein intera...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Olias G,Viollet C,Kusserow H,Epelbaum J,Meyerhof W

    更新日期:2004-06-01 00:00:00

  • Potassium-stimulated release of [3H]taurine from cultured GABAergic and glutamatergic neurons.

    abstract::The effect of depolarizing concentrations of potassium (56 mM) on the release of [3H]taurine was examined in two types of cultured neurons from mouse brain: cerebral cortex neurons, which are largely GABAergic, and cerebellar neurons, which after treatment with kainate consist almost entirely of glutamatergic granule ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schousboe A,Pasantes-Morales H

    更新日期:1989-10-01 00:00:00

  • Effects of ischemic post-conditioning on neuronal VEGF regulation and microglial polarization in a rat model of focal cerebral ischemia.

    abstract::Ischemic postconditioning is increasingly being investigated as a therapeutic approach for cerebral ischemia. However, the majority of studies are focused on the acute protection of neurons per se. Whether and how postconditioning affects multiple cells in the recovering neurovascular unit remains to be fully elucidat...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Esposito E,Hayakawa K,Ahn BJ,Chan SJ,Xing C,Liang AC,Kim KW,Arai K,Lo EH

    更新日期:2018-07-01 00:00:00

  • Changes in heat shock protein 70 and ubiquitin mRNA levels in C1300 N2A mouse neuroblastoma cells following treatment with iron.

    abstract::We have shown that following heat shock (42.5 degree C for 30 min), mouse-derived C1300 N2A neuroblastoma cells contain increased levels of mRNA coding for the inducible form of heat shock protein 70 and for ubiquitin. Incubation of C1300 cells with iron also induces an elevation in content of mRNAs coding for the sam...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Uney JB,Anderton BH,Thomas SM

    更新日期:1993-02-01 00:00:00

  • Ethanol-induced aspartate and taurine release from primary astrocyte cultures.

    abstract::Exposure of primary astrocyte cultures to isosmotic ethanol from 10-100 mM led to both swelling of the cells and release of [3H]taurine and D-[3H]aspartate. Exposure to hyperosmotic ethanol, in the same concentration range, caused neither swelling nor release. Release was inhibited by the anion transport blocker L-644...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kimelberg HK,Cheema M,O'Connor ER,Tong H,Goderie SK,Rossman PA

    更新日期:1993-05-01 00:00:00

  • Geranylgeranyl pyrophosphate is crucial for neuronal survival but has no special role in Purkinje cell degeneration in Niemann Pick type C1 disease.

    abstract::Niemann Pick type C (NPC1) is a rare fatal hereditary cholesterol storage disease associated with a massive Purkinje cells loss. The mechanisms leading to neurodegeneration are still poorly understood. Different laboratories pointed to hypersensitivity to cytotoxic effects of statins (HMG-CoA reductase inhibitors) in ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Marschalek N,Albert F,Afshordel S,Meske V,Eckert GP,Ohm TG

    更新日期:2015-04-01 00:00:00

  • Amino acid determinants for NMDA receptor inhibition by conantokin-T.

    abstract::Several derivatives of conantokin-T (con-T), a naturally occurring, gamma-carboxyglutamate (Gla)-containing peptide with NMDA receptor (NMDAR) antagonist properties, were synthesized and evaluated for their ability to displace [(3)H]MK-801 from adult rat forebrain membranes. Analyses of progressive C-terminal truncati...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Warder SE,Blandl T,Klein RC,Castellino FJ,Prorok M

    更新日期:2001-05-01 00:00:00

  • Widespread neuronal expression of branched-chain aminotransferase in the CNS: implications for leucine/glutamate metabolism and for signaling by amino acids.

    abstract::Transamination of the branched-chain amino acids produces glutamate and branched-chain alpha-ketoacids. The reaction is catalyzed by branched-chain aminotransferase (BCAT), of which there are cytosolic and mitochondrial isoforms (BCATc and BCATm). BCATc accounts for 70% of brain BCAT activity, and contributes at least...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: García-Espinosa MA,Wallin R,Hutson SM,Sweatt AJ

    更新日期:2007-03-01 00:00:00

  • Distribution of three enzymes of gamma-aminobutyric acid metabolism in monkey retina.

    abstract::The distributions of glutamate decarboxylase (EC, gamma-aminobutyric acid transaminase (EC, and succinate semialdehyde dehydrogenase (EC were determined in monkey retina. The decarboxylase was almost restricted to the inner plexiform layer. The transaminase was also highest in this layer,...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pusateri ME,Carter JG,Berger SJ,Lowry OH

    更新日期:1984-05-01 00:00:00

  • Tanycytes: Gateway to the hungry brain: An Editorial for "The FGF2-induced tanycyte proliferation involves a connexin 43 hemichannel/purinergic-dependent pathway" on

    abstract::Tanycytes are glial cells in the hypothalamus that are functionally part of the blood-brain barrier. They can sense nutrients and metabolites in the circulation such as glucose, then signal to neuronal systems to influence ingestive behaviour and energy storage, and ultimately affect body weight. The complex structure...

    journal_title:Journal of neurochemistry

    pub_type: 社论


    authors: Ebling FJP

    更新日期:2020-11-09 00:00:00

  • Dopamine D4 receptor-mediated regulation of rod opsin mRNA expression in tiger salamander.

    abstract::Light stimulates dopamine release in the retina and has been shown to rapidly up-regulate rod opsin mRNA. In the present study, we tested the effect of dopamine on rod opsin mRNA expression and examined the hypothesis that dopamine can mediate a light-evoked increase in opsin gene expression. Northern blots showed tha...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Alfinito PD,Townes-Anderson E

    更新日期:2001-02-01 00:00:00

  • Kainate receptor-mediated depression of glutamatergic transmission involving protein kinase A in the lateral amygdala.

    abstract::Kainate receptors (KARs) have been described as modulators of synaptic transmission at different synapses. However, this role of KARs has not been well characterized in the amygdala. We have explored the effect of kainate receptor activation at the synapse established between fibers originating at medial geniculate nu...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Negrete-Díaz JV,Duque-Feria P,Andrade-Talavera Y,Carrión M,Flores G,Rodríguez-Moreno A

    更新日期:2012-04-01 00:00:00

  • The alpha 1 and alpha 6 subunits can coexist in the same cerebellar GABAA receptor maintaining their individual benzodiazepine-binding specificities.

    abstract::Two GABAA receptor subunit-specific antibodies anti-alpha 6 and anti-alpha 1 have been used for elucidating the relationship between the presence of alpha 1 and/or alpha 6 subunits in the cerebellar GABAA receptors and the benzodiazepine-binding specificity. Receptor immunoprecipitation with the subunit-specific antib...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Khan ZU,Gutiérrez A,De Blas AL

    更新日期:1996-02-01 00:00:00

  • Resveratrol mitigates lipopolysaccharide- and Aβ-mediated microglial inflammation by inhibiting the TLR4/NF-κB/STAT signaling cascade.

    abstract::Activation of microglia, the resident macrophages of the brain, around the amyloid plaques is a key hallmark of Alzheimer's disease (AD). Recent evidence in mouse models indicates that microglia are required for the neurodegenerative process of AD. Amyloid-β (Aβ) peptides, the core components of the amyloid plaques, c...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Capiralla H,Vingtdeux V,Zhao H,Sankowski R,Al-Abed Y,Davies P,Marambaud P

    更新日期:2012-02-01 00:00:00

  • Gene-environment interactions in sporadic Parkinson's disease.

    abstract::Much has been learned in recent years about the genetics of familial Parkinson's disease. However, far less is known about those malfunctioning genes which contribute to the emergence and/or progression of the vast majority of cases, the 'sporadic Parkinson's disease', which is the focus of our current review. Drastic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Benmoyal-Segal L,Soreq H

    更新日期:2006-06-01 00:00:00

  • Tau promoter confers neuronal specificity and binds Sp1 and AP-2.

    abstract::Tau, a microtubule-associated protein, is encoded by a single gene, whose expression is primarily neuronal. In this work, we defined an 80-bp region of the tau promoter that confers tau protein with neuronal expression. This fragment works in conjunction with an endogenous initiation region to activate neuronal precur...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Heicklen-Klein A,Ginzburg I

    更新日期:2000-10-01 00:00:00

  • Glial uptake of neurotransmitter glutamate from the extracellular fluid studied in vivo by microdialysis and (13)C NMR.

    abstract::Glial uptake of neurotransmitter glutamate (GLU) from the extracellular fluid was studied in vivo in rat brain by (13)C NMR and microdialysis combined with gas-chromatography/mass-spectrometry. Brain GLU C5 was (13)C enriched by intravenous [2,5-(13)C]glucose infusion, followed by [(12)C]glucose infusion to chase (13)...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kanamori K,Ross BD,Kondrat RW

    更新日期:2002-11-01 00:00:00

  • Inhibitory effects of nitric oxide on the uptake of [3H]dopamine and [3H]glutamate by striatal synaptosomes.

    abstract::Ten-minute incubation with 1 mM S-nitroso-L-cysteine (NO-CYS), a nitric oxide (NO) generator, induced a 3.5-fold increase in the basal dopamine (DA) efflux from rat striatal slices. Reduced hemoglobin (100 microM), a NO scavenger, blocked this response. Nomifensine (10 microM), an inhibitor of high-affinity DA transpo...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lonart G,Johnson KM

    更新日期:1994-12-01 00:00:00

  • Cell-free synthesis of the myelin basic proteins in a wheat germ system programmed with brain messenger RNA.

    abstract::Poly A(+) messenger RNA (mRNA) was isolated from the brains of 3-week-old mice and translated in a cell-free system derived from wheat germ. Maximal stimulation of the system by brain mRNA was observed at a relatively low K+ concentration (45 mM) and low mRNA concentration (1-10 microgram/ml). The translational system...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Matthees J,Campagnoni AT

    更新日期:1980-10-01 00:00:00

  • Galactosyltransferase defects in reeler mouse brains.

    abstract::Galactosyltransferase activities were examined in the cerebellum, cerebral cortex, and brain stem of reeler and wild-type mice. Galactosyltransferase assays were optimal for all required substrates, linear with incubation time, and proportional to protein concentration. In brain areas affected by the reeler mutation (...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Shur BD

    更新日期:1982-07-01 00:00:00

  • Heat stress induces changes in protein synthesis and fast axonal transport in bullfrog sensory neurons.

    abstract::The effects of heat stress on protein synthesis and fast axonal transport were examined in an in vitro bullfrog primary afferent neuron preparation. The magnitude of effect was determined for individual [35S]methionine-labelled protein species separated via two-dimensional gel electrophoresis. Elevation of temperature...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Stone GC,Dougher MM

    更新日期:1988-09-01 00:00:00

  • Lazaroid treatment prevents death of cultured rat embryonic mesencephalic neurons following glutathione depletion.

    abstract::Reactive oxygen species are believed to play a crucial role in situations where dopamine neurons die, such as in Parkinson's disease or during intracerebral transplantation of embryonic mesencephalic tissue. The present study was designed to address the question whether, and to what extent, the glutathione redox syste...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Grasbon-Frodl EM,Andersson A,Brundin P

    更新日期:1996-10-01 00:00:00

  • Protein kinase C activity regulates D-serine availability in the brain.

    abstract::D-serine is a co-agonist of NMDA receptor (NMDAR) and plays important roles in synaptic plasticity mechanisms. Serine racemase (SR) is a brain-enriched enzyme that converts L-serine to D-serine. SR interacts with the protein interacting with C-kinase 1 (PICK1), which is known to direct protein kinase C (PKC) to its ta...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Vargas-Lopes C,Madeira C,Kahn SA,Albino do Couto I,Bado P,Houzel JC,De Miranda J,de Freitas MS,Ferreira ST,Panizzutti R

    更新日期:2011-01-01 00:00:00

  • Cerebral vulnerability is associated with selective increase in extracellular glutamate concentration in experimental thiamine deficiency.

    abstract::Microdialysis in the awake, freely moving rat was used to determine the effect of pyrithiamine-induced thiamine deficiency on the levels of amino acids in the brain. Studies were carried out on (a) presymptomatic animals immediately before the development of behavioral changes and (b) acute symptomatic animals within ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hazell AS,Butterworth RF,Hakim AM

    更新日期:1993-09-01 00:00:00

  • Combined treatment with the histone deacetylase inhibitor LBH589 and a splice-switch antisense oligonucleotide enhances SMN2 splicing and SMN expression in Spinal Muscular Atrophy cells.

    abstract::Spinal muscular atrophy (SMA) is a motor neuron disease caused by loss of function mutations in the Survival Motor Neuron 1 (SMN1) gene and reduced expression of the SMN protein, leading to spinal motor neuron death, muscle weakness and atrophy. Although humans harbour the highly homologous SMN2 gene, its defective sp...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pagliarini V,Guerra M,Di Rosa V,Compagnucci C,Sette C

    更新日期:2020-04-01 00:00:00

  • Inhibition of the chick pineal rhythm in rate of thymidine incorporation by cyclic AMP.

    abstract::Chick pineal glands in organ culture showed a circadian rhythm in the rate of thymidine incorporation. Thymidine incorporation was very markedly inhibited when 3-isobutyl-l-methylxanthine (IBMX) was continuously present. When IBMX was added to cultures in control medium during the photoperiod of the second day in cult...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wainwright SD,Wainwright LK

    更新日期:1991-05-01 00:00:00

  • Semaphorin4F interacts with the synapse-associated protein SAP90/PSD-95.

    abstract::Semaphorins are a family of secreted and membrane-associated proteins involved in growth cone guidance during development. Here, we describe the interaction of Semaphorin4F (Sema4F) with the post-synaptic density protein SAP90/PSD-95. Using the yeast two-hybrid system and coprecipitation assays we were able to show an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schultze W,Eulenburg V,Lessmann V,Herrmann L,Dittmar T,Gundelfinger ED,Heumann R,Erdmann KS

    更新日期:2001-08-01 00:00:00

  • 1-trichloromethyl-1,2,3,4-tetrahydro-beta-carboline-induced apoptosis in the human neuroblastoma cell line SK-N-SH.

    abstract::Trichloroethylene, a common industrial solvent and a metabolic precursor of chloral hydrate, occurs widely in the environment. Chloral hydrate, which is also used as a hypnotic, has been found to condense spontaneously with tryptamine, in vivo, to give rise to a highly unpolar 1-trichloromethyl-1,2,3,4-tetrahydro-beta...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Akundi RS,Macho A,Muñoz E,Lieb K,Bringmann G,Clement HW,Hüll M,Fiebich BL

    更新日期:2004-10-01 00:00:00

  • Mechanisms of neuronal migration in the adult brain.

    abstract::Adult neurogenesis was first observed nearly 60 years ago, and it has since grown into an important neurochemistry research field. Much recent research has focused on the treatment of brain diseases through neuronal regeneration with endogenously generated neurons. In the adult brain, immature neurons called neuroblas...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Kaneko N,Sawada M,Sawamoto K

    更新日期:2017-06-01 00:00:00