Antibody recognition of calcium-binding proteins depends on their calcium-binding status.


:Previous studies have revealed changes in immunohistochemical stains for calcium-binding proteins after manipulations that influence intracellular calcium. Cases have been revealed in which these changes in immunoreactivity were not correlated with changes in protein amounts. The present experiments examined whether these effects might be explained by changes in antiserum recognition due to calcium-induced changes in protein conformation. Calretinin, calbindin D28k, and parvalbumin incubated in high calcium were recognized by antisera better than when they were incubated in low calcium. Using a calbindin D28k antibody, it was shown that this effect occurs within physiological calcium concentrations. Formalin fixation of the proteins in the presence of calcium resulted in greater antibody recognition than did fixation of proteins in calcium-free states. The calretinin antiserum appeared to recognize a portion of the molecule previously shown to undergo calcium-dependent conformational changes. A calcium-insensitive antiserum was made to a different fragment of calretinin. These results indicate that some antibodies to calcium-binding proteins preferentially recognize particular calcium-induced protein conformations. Given the potential for wide fluctuations in neuronal calcium, the present results indicate that quantitative estimates of intracellular calcium-binding proteins obtained from immunohistochemical studies of neurons must be interpreted with caution.


J Neurochem


Winsky L,Kuźnicki J




Has Abstract


1996-02-01 00:00:00












  • Alternative splicing isoform of tau protein kinase I/glycogen synthase kinase 3beta.

    abstract::Glycogen synthase kinase 3 (GSK3) plays important roles in Wnt and insulin signaling, cell fate determination, and Alzheimer-like tau phosphorylation. We discovered an isoform of tau protein kinase I (TPKI)/GSK3beta with a 13 amino acid insert in the catalytic domain owing to alternative splicing. The alternative tran...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Mukai F,Ishiguro K,Sano Y,Fujita SC

    更新日期:2002-06-01 00:00:00

  • Induction of mitochondrial oxidative stress in astrocytes by nitric oxide precedes disruption of energy metabolism.

    abstract::Inhibition of the mitochondrial electron transport chain (ETC) ultimately limits ATP production and depletes cellular ATP. However, the individual complexes of the ETC in brain mitochondria need to be inhibited by approximately 50% before causing significant depression of ATP synthesis. Moreover, the ETC is the key si...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Jacobson J,Duchen MR,Hothersall J,Clark JB,Heales SJ

    更新日期:2005-10-01 00:00:00

  • Am5-HT7: molecular and pharmacological characterization of the first serotonin receptor of the honeybee (Apis mellifera).

    abstract::The biogenic amine serotonin (5-HT) plays a key role in the regulation and modulation of many physiological and behavioural processes in both vertebrates and invertebrates. These functions are mediated through the binding of serotonin to its receptors, of which 13 subtypes have been characterized in vertebrates. We ha...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schlenstedt J,Balfanz S,Baumann A,Blenau W

    更新日期:2006-09-01 00:00:00

  • Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases.

    abstract::Ts65Dn mice, trisomic for a portion of chromosome 16 segmentally homologous to human chromosome 21, are an animal model for Down's syndrome and related neurodegenerative diseases, such as dementia of the Alzheimer type. In these mice, cognitive deficits and alterations in number of basal forebrain cholinergic neurons ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Contestabile A,Fila T,Bartesaghi R,Contestabile A,Ciani E

    更新日期:2006-04-01 00:00:00

  • Mammalian forebrain ketimine reductase identified as μ-crystallin; potential regulation by thyroid hormones.

    abstract::Ketimine reductase (E.C. was purified to apparent homogeneity from lamb forebrain by means of a rapid multi-step chromatography protocol. The purified enzyme was identified by MS/MS (mass spectrometry) as μ-crystallin. The identity was confirmed by heterologously expressing human μ-crystallin in Escherichia ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hallen A,Cooper AJ,Jamie JF,Haynes PA,Willows RD

    更新日期:2011-08-01 00:00:00

  • Brain beta-spectrin phosphorylation: phosphate analysis and identification of threonine-347 as a heparin-sensitive protein kinase phosphorylation site.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sihag RK

    更新日期:1998-11-01 00:00:00

  • Zinc ions stabilise the association of basic protein with brain myelin membranes.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Earl C,Chantry A,Mohammad N,Glynn P

    更新日期:1988-09-01 00:00:00

  • Effects of ATP and taurine on calcium uptake by membrane preparations of the rat retina.

    abstract::The effects of ATP and taurine on the kinetics of calcium uptake in rat retinal membrane preparations were determined. ATP increased calcium uptake at low calcium ion concentrations. Addition of ATP plus taurine further increased calcium uptake. Cooperative relationships were observed for calcium uptake in the absence...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lombardini JB

    更新日期:1983-02-01 00:00:00

  • Identification and characterization of the rat M1 muscarinic receptor promoter.

    abstract::Five subtypes of the muscarinic receptor have been cloned from both the rat and human genomes. Although all five genes have the coding sequences in a single exon, their structures 5' of the initiation codon are largely uncharacterized, except for the M4 receptor. In the brain, muscarinic receptors mediate motor and me...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Klett CP,Bonner TI

    更新日期:1999-03-01 00:00:00

  • Involvement of OCTN2 and B0,+ in the transport of carnitine through an in vitro model of the blood-brain barrier.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Berezowski V,Miecz D,Marszałek M,Bröer A,Bröer S,Cecchelli R,Nałecz KA

    更新日期:2004-11-01 00:00:00

  • Evidence for an association of prion protein and sphingolipid-mediated signaling.

    abstract::The physiological function of the cellular prion protein (PrP(c)) is unclear. PrP(c) associates with lipid rafts, highly glycolipid-rich membrane domains containing a large variety of signaling molecules, e.g., sphingolipids (SL). In this study, we investigated possible connections between PrP(c) and sphingolipid-asso...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schmalzbauer R,Eigenbrod S,Winoto-Morbach S,Xiang W,Schütze S,Bertsch U,Kretzschmar HA

    更新日期:2008-08-01 00:00:00

  • Nicotinic modulation of [3H]dopamine release from striatal synaptosomes: pharmacological characterisation.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rapier C,Lunt GG,Wonnacott S

    更新日期:1990-03-01 00:00:00

  • Time course changes in the dopaminergic nigrostriatal system following transection of the medial forebrain bundle: detection of oxidatively modified proteins in substantia nigra.

    abstract::We studied the time course of oxidatively modified proteins in the nigrostriatal dopaminergic system following transection of the medial forebrain bundle by quantifying the number of carbonyl groups coupled to striatal and nigral protein homogenates, an index of metal-catalyzed oxidations. We found a striking effect o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Venero JL,Revuelta M,Cano J,Machado A

    更新日期:1997-06-01 00:00:00

  • The emerging role of epigenetic modifications and chromatin remodeling in spinal muscular atrophy.

    abstract::As the leading genetic cause for infantile death, Spinal Muscular Atrophy (SMA) has been extensively studied since its first description in the early 1890s. Though today much is known about the cause of the disease, a cure or effective treatment is not currently available. Recently the short chain fatty acid valproic ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Lunke S,El-Osta A

    更新日期:2009-06-01 00:00:00

  • How is alpha-synuclein cleared from the cell?

    abstract::The levels and conformers of alpha-synuclein are critical in the pathogenesis of Parkinson's Disease and related synucleinopathies. Homeostatic mechanisms in protein degradation and secretion have been identified as regulators of alpha-synuclein at different stages of its intracellular trafficking and transcellular pr...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Stefanis L,Emmanouilidou E,Pantazopoulou M,Kirik D,Vekrellis K,Tofaris GK

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  • Muscarinic and nicotinic cholinergic binding sites in the terminal abdominal ganglion of the cricket (Acheta domesticus).

    abstract::Cricket (Acheta domesticus) terminal abdominal ganglia (TG) contain high concentrations (approximately 2 pmol/mg protein) of muscarinic and nicotinic cholinergic binding sites, based on the capacity of TG to bind specifically the labelled ligands L-[3H]quinuclidinyl benzilate ([3H]QNB) and [125I]alpha-bungarotoxin ([1...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Meyer MR,Reddy GR

    更新日期:1985-10-01 00:00:00

  • Phosphorylation and transcriptional activity regulation of retinoid-related orphan receptor alpha 1 by protein kinases C.

    abstract::Retinoid-related orphan receptor alpha1 (RORalpha1) is a member of the nuclear receptor superfamily. It is highly expressed in CNS particularly in the cerebellum. Absence of this transcription factor in mice leads to several abnormalities, such as cerebellar atrophy linked to Purkinje cell death and impaired different...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Duplus E,Gras C,Soubeyre V,Vodjdani G,Lemaigre-Dubreuil Y,Brugg B

    更新日期:2008-03-01 00:00:00

  • Tachykinins potentiate N-methyl-D-aspartate responses in acutely isolated neurons from the dorsal horn.

    abstract::Substance P and neurokinin A both potentiated N-methyl-D-aspartate (NMDA)-induced currents recorded in acutely isolated neurons from the dorsal horn of the rat. To elucidate the mechanism underlying this phenomenon, we measured the effects of tachykinins and glutamate receptor agonists on [Ca2+]i in these cells. Subst...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rusin KI,Bleakman D,Chard PS,Randic M,Miller RJ

    更新日期:1993-03-01 00:00:00

  • An astrocyte-specific enhancer of the aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements.

    abstract::Aquaporin-4, a predominant water channel in the brain, is specifically expressed in astrocyte endfeet and plays a central role in water homeostasis, neuronal activity, and cell migration in the brain. It has two dominant isoforms called M1 and M23, whose mRNA is driven by distinct promoters located upstream of exons 0...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Abe Y,Ikeshima-Kataoka H,Goda W,Niikura T,Yasui M

    更新日期:2012-03-01 00:00:00

  • Characterization of a dipeptidyl aminopeptidase from bovine adrenal medulla.

    abstract::A dipeptidyl aminopeptidase was partially purified from a supernatant fraction of bovine adrenal medulla by gel filtration and anion-exchange chromatography. From gel filtration, the apparent molecular weight of the enzyme was 68,100 and its pH optimum was 9.5. Its Km for hydrolysis of the synthetic substrate arginyla...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Kecorius E,Small DH,Livett BG

    更新日期:1988-01-01 00:00:00

  • Characterization of the thyroid hormone transport system of cerebrocortical rat neurons in primary culture.

    abstract::The uptake of 3',3,5-triiodo-L-thyronine (T3) and L-thyroxine (T4) by primary cultures derived from rat brain hemispheres was studied under initial velocity conditions, at 25 degrees C. Uptake of both hormones was carrier mediated and obeyed simple Michaelis-Menten kinetics. The Km of T3 uptake was very similar to tha...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chantoux F,Blondeau JP,Francon J

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  • Peripheral adipose tissue insulin resistance alters lipid composition and function of hippocampal synapses.

    abstract::Compelling evidence indicates that type 2 diabetes mellitus, insulin resistance (IR), and metabolic syndrome are often accompanied by cognitive impairment. However, the mechanistic link between these metabolic abnormalities and CNS dysfunction requires further investigations. Here, we evaluated whether adipose tissue ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sallam HS,Tumurbaatar B,Zhang WR,Tuvdendorj D,Chandalia M,Tempia F,Laezza F,Taglialatela G,Abate N

    更新日期:2015-04-01 00:00:00

  • Effects of 3,4-methylenedioxymethamphetamine (MDMA) on serotonin transporter and vesicular monoamine transporter 2 protein and gene expression in rats: implications for MDMA neurotoxicity.

    abstract::3,4-Methylenedioxymethamphetamine (MDMA; 'Ecstasy') is a popular recreational drug used worldwide. This study aimed to determine the effects of this compound on the expression of nerve terminal serotonergic markers in rats. Experiment 1 investigated MDMA-induced changes in levels of the serotonin transporter (SERT) an...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Biezonski DK,Meyer JS

    更新日期:2010-02-01 00:00:00

  • Multiple levels for regulation of TrkA in PC12 cells by nerve growth factor.

    abstract::TrkA is a receptor tyrosine kinase for nerve growth factor (NGF). Recent studies indicate that NGF regulates not only activation of trkA kinase but also expression of the trkA gene. To further define NGF actions on trkA, we examined binding and signaling through trkA after both short and long intervals of NGF treatmen...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zhou J,Valletta JS,Grimes ML,Mobley WC

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  • Synaptic concentration of dopamine in the mouse striatum in relationship to the kinetic properties of the dopamine receptors and uptake mechanism.

    abstract::The concentration of dopamine (DA) in the synaptic cleft in the mouse striatum in vivo was estimated from the competition between the synaptic DA and the 3H-labelled DA D2 receptor agonists N-n-propylnorapomorphine (NPA) or N,N-diethyl-N'-[(3 alpha, 4a alpha, 10 beta)-1,2,3,4,4a,5,10,10a-octahydro- 7-hydroxyl-1-propyl...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ross SB

    更新日期:1991-01-01 00:00:00

  • Impaired fast axonal transport in neurons of the sciatic nerves from dystonia musculorum mice.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: De Repentigny Y,Deschênes-Furry J,Jasmin BJ,Kothary R

    更新日期:2003-08-01 00:00:00

  • Indoleamine-sensitive adenylate cyclase in rabbit retina: characterization and distribution.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Blazynski C,Ferrendelli JA,Cohen AI

    更新日期:1985-08-01 00:00:00

  • Mitigation of peroxynitrite-mediated nitric oxide (NO) toxicity as a mechanism of induced adaptive NO resistance in the CNS.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bishop A,Gooch R,Eguchi A,Jeffrey S,Smallwood L,Anderson J,Estevez AG

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  • Zebrafish genes for neuropeptide Y and peptide YY reveal origin by chromosome duplication from an ancestral gene linked to the homeobox cluster.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Söderberg C,Wraith A,Ringvall M,Yan YL,Postlethwait JH,Brodin L,Larhammar D

    更新日期:2000-09-01 00:00:00

  • Synaptotagmin I is a molecular target for lead.

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    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bouton CM,Frelin LP,Forde CE,Arnold Godwin H,Pevsner J

    更新日期:2001-03-01 00:00:00