Recognition of differentiation antigens of spermatogenesis in the mouse by using antibodies from spleen cell-myeloma hybrids after syngeneic immunization.

abstract：:Granulosa cells from immature hypophysectomized diethylstilbestrol-treated rats displayed pronounced intracellular and intercellular changes after 48 hr of exposure to follicle-stimulating hormone (FSH) in vitro. As determined by light and electron microscopy, most of the FSH-treated cells became highly aggregated and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amsterdam A,Knecht M,Catt KJ

更新日期：1981-05-01 00:00:00

abstract：:Treatment with neuraminidase (NA) plus galactose oxidase (GalOxase) does not cause stimulation of human thymocytes. However, stimulation can be achieved by addition of exogenous interleukin 2 (IL-2). The IL-2-induced stimulation was inhibited with anti-Tac antibody, indicating that NA/GalOxase-oxidized cells can serve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roffman E,Sredni B,Smolinsky A,Wilchek M

更新日期：1984-08-01 00:00:00

abstract：:Conflicting data have been reported with regard to the infectability, dysfunction, and depletion of dendritic cells (DCs) in human immunodeficiency virus (HIV) disease. These discrepancies could potentially be explained by the existence of multiple subsets of cells with dendritic morphology in peripheral blood. The is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weissman D,Li Y,Ananworanich J,Zhou LJ,Adelsberger J,Tedder TF,Baseler M,Fauci AS

更新日期：1995-01-31 00:00:00

abstract：:The expression of CD5 increases progressively as thymocytes mature. We have shown that CD5 expression is controlled by a tissue-specific regulatory promoter located upstream of the CD5 translation start sites. Deletion of this regulatory promoter, which contains three potential transcription factor binding sites (CCAA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Contag CH,Felsher D,Shachaf CM,Cao Y,Herzenberg LA,Herzenberg LA,Tung JW

更新日期：2004-03-16 00:00:00

abstract：:The demonstration of enzymatic capabilities of certain RNAs, in addition to their well-known template properties, has led to the recognition that RNAs are the only biological macromolecules that can function both as genotype and phenotype, hence raising the possibility of Darwinian selection and precellular evolution ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Diener TO

更新日期：1989-12-01 00:00:00

abstract：:A 1,942-base-pair DNA segment encoding the structural gene for diphtheria toxin was sequenced, and the primary structure of the toxin was deduced. Restriction enzyme fragments corresponding to nontoxic or hypotoxic peptides of the toxin were isolated from corynebacteriophage beta and cloned into Escherichia coli on pl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greenfield L,Bjorn MJ,Horn G,Fong D,Buck GA,Collier RJ,Kaplan DA

更新日期：1983-11-01 00:00:00

abstract：:Antigen receptor variable region exons are assembled during lymphocyte development from variable (V), diversity (D), and joining (J) gene segments. Each germ-line gene segment is flanked by recombination signal sequences (RSs). Recombination-activating gene endonuclease initiates V(D)J recombination by cleaving a pair...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zha S,Jiang W,Fujiwara Y,Patel H,Goff PH,Brush JW,Dubois RL,Alt FW

更新日期：2011-02-01 00:00:00

abstract：:Antimicrobial levels of reactive oxygen species (ROS) are produced by the mammalian host defense to kill invading bacteria and limit bacterial colonization. One main in vivo target of ROS is the thiol group of proteins. We have developed a quantitative thiol trapping technique termed OxICAT to identify physiologically...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leichert LI,Gehrke F,Gudiseva HV,Blackwell T,Ilbert M,Walker AK,Strahler JR,Andrews PC,Jakob U

更新日期：2008-06-17 00:00:00

abstract：:In previous papers of this series, emphasis has been placed on the steady-state phase transition and critical properties of large lattices of interacting, symmetrical, and identical enzyme molecules. The present paper is concerned with a number of examples of enzyme--enzyme interactions that do not belong to the class...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1978-03-01 00:00:00

abstract：:"Evolution behaves like a tinkerer" (François Jacob, Science, 1977). Software systems provide a singular opportunity to understand biological processes using concepts from network theory. The Debian GNU/Linux operating system allows us to explore the evolution of a complex network in a unique way. The modular design d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fortuna MA,Bonachela JA,Levin SA

更新日期：2011-12-13 00:00:00

abstract：:We show that the diverse ecoregions of Madagascar share one distinctive climatic feature: unpredictable intra- or interannual precipitation compared with other regions with comparable rainfall. Climatic unpredictability is associated with unpredictable patterns of fruiting and flowering. It is argued that these featur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dewar RE,Richard AF

更新日期：2007-08-21 00:00:00

abstract：:Nucleotide variations, including SNPs, in the coding regions of disease genes are important targets for RNAi treatment, which is a promising medical treatment for intractable diseases such as triplet repeat diseases. However, the identification of such nucleotide variations and the design of siRNAs conferring disease ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takahashi M,Watanabe S,Murata M,Furuya H,Kanazawa I,Wada K,Hohjoh H

更新日期：2010-12-14 00:00:00

abstract：:Hypophosphatasia is an inherited disorder characterized by defective bone mineralization and a deficiency of serum and tissue liver/bone/kidney alkaline phosphatase (L/B/K ALP) activity. Clinical severity is variable, ranging from death in utero (due to severe rickets) to pathologic fractures first presenting in adult...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weiss MJ,Cole DE,Ray K,Whyte MP,Lafferty MA,Mulivor RA,Harris H

更新日期：1988-10-01 00:00:00

abstract：:We have developed a reliable genetic selection strategy for isolating interacting proteins based on the "hitchhiker" mechanism of the Escherichia coli twin-arginine translocation (Tat) pathway. This method, designated FLI-TRAP (functional ligand-binding identification by Tat-based recognition of associating proteins),...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waraho D,DeLisa MP

更新日期：2009-03-10 00:00:00

abstract：:Systemic anthrax, caused by inhalation or ingestion of Bacillus anthracis spores, is characterized by rapid microbial growth stages that require iron. Tightly bound and highly regulated in a mammalian host, iron is scarce during an infection. To scavenge iron from its environment, B. anthracis synthesizes by independe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abergel RJ,Wilson MK,Arceneaux JE,Hoette TM,Strong RK,Byers BR,Raymond KN

更新日期：2006-12-05 00:00:00

abstract：:Hermansky-Pudlak syndrome (HPS) defines a group of autosomal recessive disorders characterized by deficiencies in lysosome-related organelles such as melanosomes and platelet-dense granules. Several HPS genes encode proteins of unknown function including HPS1, HPS3, and HPS4. Here we have identified and characterized ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nazarian R,Falcón-Pérez JM,Dell'Angelica EC

更新日期：2003-07-22 00:00:00

abstract：:Ascorbate (vitamin C) recycling occurs when extracellular ascorbate is oxidized, transported as dehydroascorbic acid, and reduced intracellularly to ascorbate. We investigated microorganism induction of ascorbate recycling in human neutrophils and in microorganisms themselves. Ascorbate recycling was determined by mea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Russo TA,Kwon O,Chanock S,Rumsey SC,Levine M

更新日期：1997-12-09 00:00:00

abstract：:The atomic buckling in 2D "Xenes" (such as silicene) fosters a plethora of exotic electronic properties such as a quantum spin Hall effect and could be engineered by external strain. Quantifying the buckling magnitude with subangstrom precision is, however, challenging, since epitaxially grown 2D layers exhibit comple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pawlak R,Drechsel C,D'Astolfo P,Kisiel M,Meyer E,Cerda JI

更新日期：2020-01-07 00:00:00

abstract：:The protein products of T4 bacteriophage genes 41, 43, 45, 44, and 62 have been purified to near homogeneity using an assay which measures their stimulation of DNA synthesis in a crude lysate of Escherichia coli cells in fected by an appropriate mutant phage. When all of these proteins and T4 gene 32 protein are incub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morris CF,Sinha NK,Alberts BM

更新日期：1975-12-01 00:00:00

abstract：:Dimension reduction methods are commonly applied to high-throughput biological datasets. However, the results can be hindered by confounding factors, either biological or technical in origin. In this study, we extend principal component analysis (PCA) to propose AC-PCA for simultaneous dimension reduction and adjustme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lin Z,Yang C,Zhu Y,Duchi J,Fu Y,Wang Y,Jiang B,Zamanighomi M,Xu X,Li M,Sestan N,Zhao H,Wong WH

更新日期：2016-12-20 00:00:00

abstract：:Molecules of transfer RNA (tRNA) typically contain four stems composed of Watson-Crick (W-C) base pairs and infrequent mispairs such as G-U and A-C. The latter mispairs are fundamental units of RNA secondary structure found in nearly every class of RNA and are nearly isomorphic to W-C pairs. Therefore, they often subs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain WH

更新日期：2006-03-21 00:00:00

abstract：:We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz F,Maeda N,Smithies O,Hickey R,Edelmann W,Skoultchi A,Kucherlapati R

更新日期：1991-12-01 00:00:00

abstract：:Most poxviruses, including variola, the causative agent of smallpox, express a secreted protein of 35 kDa, vCCI, which binds CC-chemokines with high affinity. This viral protein competes with the host cellular CC-chemokine receptors (CCRs), reducing inflammation and interfering with the host immune response. Such prot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carfí A,Smith CA,Smolak PJ,McGrew J,Wiley DC

更新日期：1999-10-26 00:00:00

abstract：:H-NS and Lsr2 are nucleoid-associated proteins from Gram-negative bacteria and Mycobacteria, respectively, that play an important role in the silencing of horizontally acquired foreign DNA that is more AT-rich than the resident genome. Despite the fact that Lsr2 and H-NS proteins are dissimilar in sequence and structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gordon BR,Li Y,Cote A,Weirauch MT,Ding P,Hughes TR,Navarre WW,Xia B,Liu J

更新日期：2011-06-28 00:00:00

abstract：:A typing method for bacteria was developed and applied to several species, including Escherichia coli and Actinobacillus actinomycetemcomitans. Total genomic DNA was digested with a restriction endonuclease, and fragments were enabled with [alpha-32P]dATP by using the Klenow fragment of DNA polymerase and separated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Steenbergen TJ,Colloms SD,Hermans PW,de Graaff J,Plasterk RH

更新日期：1995-06-06 00:00:00

abstract：:Mechanisms by which the extracellular domain of Notch1 controls Notch1 signaling are not well defined. Here, we show that the O-fucose glycan in the Notch1 ligand-binding domain regulates the strength of Notch1 signaling during embryogenesis, postweaning growth, and T cell development in the mouse. Heterozygotes carry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ge C,Stanley P

更新日期：2008-02-05 00:00:00

abstract：:Local tissue stem cells have been described in airways of the lung but their contribution to normal epithelial maintenance is currently unknown. We therefore developed aggregation chimera mice and a whole-lung imaging method to determine the relative contributions of progenitor (Clara) and bronchiolar stem cells to ep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giangreco A,Arwert EN,Rosewell IR,Snyder J,Watt FM,Stripp BR

更新日期：2009-06-09 00:00:00

abstract：:Knowledge of normal human cardiac excitation stems from isolated heart or intraoperative mapping studies under nonphysiological conditions. Here, we use a noninvasive imaging modality (electrocardiographic imaging) to study normal activation and repolarization in intact unanesthetized healthy adults under complete phy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ramanathan C,Jia P,Ghanem R,Ryu K,Rudy Y

更新日期：2006-04-18 00:00:00

abstract：:We have investigated the theory that the insulin-induced loss of insulin binding from adipocytes is due to internalization of insulin receptors. Cell-surface receptors were assessed by the binding capacity of intact cells at 16 degrees C. Total (i.e., cell-surface plus intracellular) receptors were assessed by solubil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Green A,Olefsky JM

更新日期：1982-01-01 00:00:00

abstract：:We have applied a molecular biology approach to the identification of human monoclonal antibodies. Human peripheral blood lymphocyte mRNA was converted to cDNA and a select subset was amplified by the polymerase chain reaction. These products, containing coding sequences for numerous immunoglobulin heavy- and kappa li...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mullinax RL,Gross EA,Amberg JR,Hay BN,Hogrefe HH,Kubitz MM,Greener A,Alting-Mees M,Ardourel D,Short JM

更新日期：1990-10-01 00:00:00