Abstract:
:Cannabinoids, flavonoids, and stilbenoids have been identified in the annual dioecious plant Cannabis sativa L. Of these, the cannabinoids are the best known group of this plant's natural products. Polyketide synthases (PKSs) are responsible for the biosynthesis of diverse secondary metabolites, including flavonoids and stilbenoids. Biosynthetically, the cannabinoids are polyketide substituted with terpenoid moiety. Using an RT-PCR homology search, PKS cDNAs were isolated from cannabis plants. The deduced amino acid sequences showed 51%-73% identity to other CHS/STS type sequences of the PKS family. Further, phylogenetic analysis revealed that these PKS cDNAs grouped with other non-chalcone-producing PKSs. Homology modeling analysis of these cannabis PKSs predicts a 3D overall fold, similar to alfalfa CHS2, with small steric differences on the residues that shape the active site of the cannabis PKSs.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Flores-Sanchez IJ,Linthorst HJ,Verpoorte Rdoi
10.1590/S1415-47572010005000088subject
Has Abstractpub_date
2010-10-01 00:00:00pages
703-13issue
4eissn
1415-4757issn
1678-4685journal_volume
33pub_type
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