Abstract:
:Epidermal growth factor (EGF), a multifunctional growth factor, is a regulator in a wide variety of physiological processes. EGF plays an important role in the regulation of liver regeneration. This study was aimed at investigating the methylation level of EGF gene throughout liver regeneration. DNA of liver tissue from control rats and partial hepatectomy (PH) rats at 10 time points was extracted and a 354 bp fragment including 10 CpG sites from the transcription start was amplified after DNA was modified by sodium bisulfate. The result of sequencing suggested that methylation ratio of four CpG sites was found to be significantly changed when PH group was compared to control group, in particular two of them were extremely striking. mRNA expression of EGF was down-regulated in total during liver regeneration. We think that the rat EGF promoter region is regulated by variation in DNA methylation during liver regeneration.
journal_name
Genet Mol Bioljournal_title
Genetics and molecular biologyauthors
Li D,Fan J,Li Z,Xu Cdoi
10.1590/s1415-47572014000300017subject
Has Abstractpub_date
2014-06-01 00:00:00pages
439-43issue
2eissn
1415-4757issn
1678-4685journal_volume
37pub_type
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