Abstract:
:Gamma linolenic acid (GLA) is an anti-cancer agent recently reported to inhibit tumour cell-matrix attachment. This study examined the effects of GLA on the adhesion of two tumour cell lines, HT115 (human colon) and MDA MB 231 (human breast), to an extracellular matrix, Matrigel. The action of GLA on focal adhesion kinase(FAK) and paxillin was also investigated. Following cell adhesion to Matrigel in control experiments, both FAK and paxillin were quickly tyrosine phosphorylated and become concentrated at focal adhesion areas. Inclusion of GLA resulted in an inhibition of the tyrosine phosphorylation of both FAK and paxillin leading to a reduced attachment of both cell types to Matrigel. FAK and paxillin were also less well distributed in the focal adhesions compared with the controls. It is concluded, therefore, that GLA inhibits tumour-matrix adhesion via the inhibition of FAK and paxillin tyrosine phosphorylation.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Jiang W,Hiscox S,Puntis M,Hallett M,Bryce R,Horrobin D,Mansel Rdoi
10.3892/ijo.8.3.583subject
Has Abstractpub_date
1996-03-01 00:00:00pages
583-7issue
3eissn
1019-6439issn
1791-2423journal_volume
8pub_type
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