Abstract:
:Expression of the tumor suppressor gene p53 and the ras oncogene were examined in 46 tumor and nodal specimens of non-small cell lung cancer (NSCLC) using the antibodies p53 pAb 240 and ras Y13-259 respectively. p53 expression was elevated in 46% and ras p21 was over-expressed in 85% of the tumor specimens analyzed. Fifteen cases of benign lessions were also assessed for both ras p21 and p53 expression; all were found to have negative staining. p53 over-expression was found to correlate with a poor prognosis in both the tumor specimens (p<0.05) and in the nodal tissues (p<0.005). Ras p21 over-expression was found to be associated with survival (p<0.1) in both the tumor and the nodal specimens. Stage of the disease correlated with survival; similarly both p53 and ras p21 over-expression correlated with stage. No correlations were found with the pathological grade of the tumors nor with a history of smoking or duration of smoking. No K-ras mutations at codon 12 were observed in a further 15 NSCLC specimens analyzed. These results indicate that the p53 gene in particular plays a role in the stages of NSCLC.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Papadakis E,Malliri A,Linardopoulos S,Karaiossifidi H,Field J,Spandidos Ddoi
10.3892/ijo.1.4.403subject
Has Abstractpub_date
1992-09-01 00:00:00pages
403-13issue
4eissn
1019-6439issn
1791-2423journal_volume
1pub_type
杂志文章abstract::From January 1992 to December 1995, 129 patients with previously untreated non-Hodgkin's lymphoma were randomised in a phase III multicenter trial to receive CEOP-B/VIMB or ProMACE-CytaBOM. Eligibility criteria included intermediate or high grade lymphoma (follicular large cell, diffuse small cleaved-cell, diffuse mix...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.3892/ijo.16.1.149
更新日期:2000-01-01 00:00:00
abstract::One of the early events of apoptosis is proteolysis. The enzyme(s) involved in this event appear to be the serine protease(s), inasmuch as the reversible or irreversible inhibitors of serine proteases prevent DNA degradation and abrogate other signs of apoptosis in several cell systems (Gorczyca et al, Int J Oncol 1: ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.4.627
更新日期:1993-10-01 00:00:00
abstract::We previously reported that podophyllotoxin acetate (PA) radiosensitizes NCI-H460 cells. Here, we confirmed that PA treatment also induces cell death among two other non-small cell lung cancer (NSCLC) cell lines: NCI-H1299 and A549 cells (IC50 values = 7.6 and 16.1 nM, respectively). Our experiments further showed tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3123
更新日期:2015-10-01 00:00:00
abstract::Two mouse HPV16-transformed cell lines, viz. MK16 cells, which induce metastasizing tumors, and TC-1 cells, which induce non-metastasizing tumors were transduced with the gene for mouse endostatin. Two clones constitutively expressing endostatin were isolated from each of them. They were denoted ME3 and ME9, and TE2 a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000331
更新日期:2009-07-01 00:00:00
abstract::DNA double-strand breaks (DSBs) are potentially mutagenic/carcinogenic lesions. Induction of DSBs triggers phosphorylation of histone H2AX on Ser-139. Phosphorylated H2AX (gammaH2AX) can be detected immunocytochemically, and the intensity of gammaH2AX immunofluorescence (IF), reflecting the number of gammaH2AX-IF foci...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::The GTPase activity of purified dimeric tubulin (alpha+beta) at 5 mu M was insensitive to methyl-3,5-diiodo-4-(4'-methoxyphenoxy) benzoate (DIME), in contrast to nocodazole which activated GTPase. Cellular motility of MDA-MB-231 (human mammary cancer) cells migrating through 12-mu m pores was inhibited by DIME similar...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1247
更新日期:1997-12-01 00:00:00
abstract::Accumulating evidence suggests that colorectal cancer (CRC) should be viewed as a heterogeneous disease, with proximal and distal CRCs showing multiple biological and clinical differences. The aim of this study was to develop a clinicopathological, molecular and protein profile for CRCs based on their region and thus ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000720
更新日期:2010-09-01 00:00:00
abstract::Acquisition of P-gp-mediated multidrug-resistance does not always correlate with observed malignant behavior of NB. To characterize alterations accompanying development of multidrug-resistance in NB we established two neuroblastoma cell sublines resistant to vincristine (UKF-NB-3rVCR10) and doxorubicin (UKF-NB-3rDOX20...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::The support mechanisms that are involved in lymph-node metastasis of oral squamous cell carcinoma (OSCC) remain largely unknown. Recent studies have demonstrated that tumor cells express chemokine receptors and use chemokines to metastasize to the target organ in many malignancies in humans. In this study, we examined...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Mesothelioma is an aggressive tumor which is mainly derived from the pleura of lung. In the present study, we evaluated the anticancer effect of suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor on human mesothelioma cells in relation to the levels of HDAC1, reactive oxygen species (ROS) a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3402
更新日期:2016-05-01 00:00:00
abstract::The aim of this study was to assess pivotal factors determining the oxygenation status of malignant tumors, and, for comparison, of normal tissues. For this purpose, microvascular patterns, blood flow data, O2 consumption rates and O2 extractions were correlated to the median pO2 values of isografted rodent and xenogr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Endometrial cancer (EC) is one of the most common malignancies of the female reproductive system, and metastasis is a major cause of mortality. In this study, we aimed to explore the role of CP‑31398 in the migration, invasion and apoptosis of EC cells by its regulation of the expression of the murine double minute 2 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4681
更新日期:2019-03-01 00:00:00
abstract::2-Phenyl-4-quinolone series compounds have exhibited growth inhibitory influence on several human cancer cell lines. In this study, we investigated the effects of 2-(3-(methylamino)phenyl)-6-(pyrrolidin-1-yl)quinolin-4-one (Smh-3) on viability, cell cycle and apoptotic cell death which occurred in different leukemia c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.952
更新日期:2011-05-01 00:00:00
abstract::The naturally occurring photosensitizer, hypericin, with its high quantum yield of singlet oxygen photogeneration was studied for its ability to differentiate between glioblastoma cells and fetal rat neurons using fluorescence microscopy. Eight human glioma cell lines and twelve primary human glioma cell cultures were...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::The promising benefits of cancer gene therapy have been limited by the inability to deliver therapeutic genes homogeneously throughout the tumor mass and to control gene expression within the tumor cells. Transcriptional targeting, the use of DNA regulatory promoter sequences to localize transgene expression, has been...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-01 00:00:00
abstract::Subsequent to the publication of the above article, the authors have realized that there were errors associated with Figs. 1c and 2b. In Fig. 1c, the authors noted that the same data were incorrectly presented for the 'Untreated cells" and 'DMSO' dot‑blot experiments. After having re‑examined their source data, the au...
journal_title:International journal of oncology
pub_type: 杂志文章,已发布勘误
doi:10.3892/ijo.2019.4874
更新日期:2019-12-01 00:00:00
abstract::The p53-induced wig-1 gene encodes an unusual nuclear zinc finger protein with high sequence similarity between human, rat and mouse. Wig-1 belongs to a group of proteins with widely spaced zinc fingers that bind double stranded (ds) RNA. We show here that wig-1 is present in Gallus gallus (chicken), Silurana tropical...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-06-01 00:00:00
abstract::The Drosophila ras2 and rop gene pair are governed by a common promoter. Characterisation of the Drosophila ras2/rop bidirectional promoter has revealed that a single major protein complex (M), composed of two subunits (factors A and B) interacts with two central promoter domains (regions A and B). To define the trans...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.5.1185
更新日期:1995-11-01 00:00:00
abstract::To investigate the relationships of the gross appearance to cancer depth and microinvasion in gallbladder cancer, thirty-three cancers were macroscopically subgrouped: papillary (n=8), nodular (n=6), papillo-invasive (n=1), nodulo-invasive (n=11), and invasive (n=7) type, respectively. Papillary type cancers were limi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Photodynamic therapy (PDT) is a tool against neoplastic and non-neoplastic diseases. PDT is capable to induce different cell death mechanisms in vitro, triggered in a dose-dependent manner. Relationships between PDT and apoptosis or necrosis induction are well-known, but other cell death mechanisms triggered after PDT...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_32_6_1189
更新日期:2008-06-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1461
更新日期:2012-07-01 00:00:00
abstract::Although epidemiological studies have suggested a positive correlation between environmental radon exposure and prostate cancer, the mechanism involved is not clear. In the present study, we examined the oncogenic transforming potency of alpha-particles using non-tumorigenic, telomerase-immortalized human benign prost...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-09-01 00:00:00
abstract::Gene expression of dihydropyrimidine dehydrogenase (DPD) and newly multidrug resistance-associated protein (MRP) was found to correlate well with primary 5-FU resistance in 7 human gastrointestinal cancer cell lines. Although mRNA and protein levels of thymidylate synthase (TS) did not relate to the resistance, 5-FU t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.3.551
更新日期:1999-03-01 00:00:00
abstract::The purpose of this study was to test the hypothesis that cooperative interaction between cutaneous fatty acid-binding protein (C-FABP) and peroxisome proliferator-activated receptors (PPAR) promotes the malignant progression of human prostate cancer. The expression of C-FABP, PPARβ/δ and PPARγ was measured by western...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2166
更新日期:2014-01-01 00:00:00
abstract::We examined the effects of suppressing multidrug resistance-associated protein (MRP) and multidrug resistance 1 (MDR1) gene expression in HCT-8DDP human colon cancer cell lines, which showed both cisplatin and multidrug resistance. Hammerhead ribozymes, designed to cleave MRP mRNA (anti-MRP Rz) and MDR1 mRNA (anti-MDR...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-11-01 00:00:00
abstract::Human squamous cell carcinomas frequently overexpress the epidermal growth factor receptor (EGFR) and this is often associated with poor prognosis in patients with these cancers. The high level of expression of the EGFR provides an important target for therapy and we and others have shown that monoclonal antibodies (m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.2.335
更新日期:1998-08-01 00:00:00
abstract::Loss of heterozygosity (LOH) or allelic deletion at various loci has been reported in the majority of human tumors. The frequently deleted targets are believed to be tumor suppressor genes. Recent studies have identified the APC and MCC genes at 5q21, as putative tumor suppressor genes. The APC and MCC genes have been...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.1.111
更新日期:1995-01-01 00:00:00
abstract::Although CD138 expression is a hallmark of plasma cells and myeloma cells, reduced CD138 expression is occasionally found. However, the mechanisms underlying CD138 downregulation in myeloma cells remain unclear. Previous reports suggest that the bone marrow microenvironment may contribute to CD138 downregulation. Amon...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2134
更新日期:2013-12-01 00:00:00
abstract::The prognosis of patients carrying glottic squamous cell carcinomas (GSCCs) involving the anterior commissure is often unpredictable. In order to assess the possible prognostic role of new and reliable parameters, p53 and cyclin D1 protein expression was immunohistochemically analysed in pathological samples from 27 p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.3.553
更新日期:1996-09-01 00:00:00
abstract::The expression of RNA for interleukin (IL) -9, -10 and -12, interferon gamma (IFN-gamma), transforming growth factor beta one (TGF-beta1), macrophage inflammatory protein one alpha (MIP-1alpha) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in a panel of human T leukemia cell lines at various stages of ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.3.633
更新日期:1994-03-01 00:00:00