Abstract:
PURPOSE:The involvement of cytokines and chemokines in vitreous fluid is important in the development and progression of diabetic retinopathy (DR) and central retinal vein occlusion (CRVO). In this study, the concentrations of cytokines and chemokines in the vitreous fluid of eyes with DR and CRVO were measured and compared. METHODS:We studied 76 eyes with proliferative DR and diabetic macular edema (DR group), 10 eyes with CRVO (CRVO group), and 23 eyes with an epiretinal membrane and macular hole (control group), among a series of 160 eyes from which vitreous fluid samples were collected during vitrectomy. The vitreous fluid samples were collected by suction with a vitreous cutter at the initial stage of vitrectomy. Twenty-seven different cytokines and chemokines were measured simultaneously using an array system (Bio-Plex(®)) with beads combined with antibodies (Bio-Rad), as follows: interleukin (IL)-1β, IL-1 receptor agonist, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, eotaxin, basic fibroblast growth factor, granulocyte colony-stimulating factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-γ, interferon-inducible 10-kDa protein (IP-10), monocytochemotactic protein-1 (MCP-1), macrophage inflammatory protein-1 alpha (MIP-1α), MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated upon activation, normal T cell expressed and secreted, tumor necrosis factor alpha (TNF-α), and vascular endothelial growth factor (VEGF). RESULTS:Compared to the control group, the levels of IL-6, IL-8, IL-10, IL-13, IP-10, MCP-1, MIP-1β, PDGF and VEGF in the vitreous fluid were significantly higher in the DR group, while the levels of IL-1β, IL-2, IL-5, IL-8, IL-9, IL-10, IL-12, IL-13, eotaxin, G-CSF, IFN-γ, IP-10, MCP-1, MIP-1β, TNF-α and VEGF were significantly higher in the CRVO group. Compared to the DR group, IL-2, IL-9, IL-12, MCP-1 and IFN-γ were significantly elevated in the CRVO group. Multivariate regression analysis revealed that among 6 factors correlated to VEGF in the DR group, IL-10 and IL-13 were more positively correlated and PDGF was most inversely correlated to VEGF. CONCLUSION:In addition to inflammatory cytokines and neurotrophic factors such as VEGF, anti-inflammatory cytokines such as IL-10 and IL-13 may be involved more in the pathogenesis of DR and CRVO than in other diseases; cytokines and chemokines may also be correlated to VEGF in the vitreous fluid. It is also suggested that the inflammatory reaction may be more activate in CRVO than in DR.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Suzuki Y,Nakazawa M,Suzuki K,Yamazaki H,Miyagawa Ydoi
10.1007/s10384-011-0004-8subject
Has Abstractpub_date
2011-05-01 00:00:00pages
256-263issue
3eissn
0021-5155issn
1613-2246pii
10.1007/s10384-011-0004-8journal_volume
55pub_type
杂志文章abstract::The value of the polymerase chain reaction (PCR) in the diagnosis of stromal herpetic keratitis was examined using tear fluid specimens; the sensitivity of the nested PCR method in detecting the herpes simplex virus (HSV) genome was 1 plaque-forming unit/mL. PCR assay of 72 tear samples from the eyes of 15 patients wi...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Two siblings with Usher syndrome had optic disc mulberry tumors that were photographically documented over a 5-year period. During this period, growth of these tumors was demonstrated. These lesions were reported histologically to be drusen. The existence of presumed optic disc drusen in siblings with Usher syndrome m...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::A new in vitro model to evaluate the corneal endothelial wound-healing process was developed. Confluent monolayer cell sheets of either rabbit or bovine corneal endothelial cells were cultured on cover slips, the surface of which had been precoated with hydroxyethyl methacrylate (HEMA) except for the 8 mm-diameter are...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:Interferon alpha-2b (IFN alpha 2b) was recently shown to be effective as an alternative therapy for conjunctival intraepithelial neoplasia (CIN). Herein, we report our successful management of CIN by the use of IFN alpha 2b. CASE:A 73-year-old woman presented with a complaint of irritation due to a tumorous...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00494-4
更新日期:2002-03-01 00:00:00
abstract:PURPOSE:To elucidate the characterization of intraocular pressure (IOP) spike after trabeculotomy, and after the combined procedure of phacoemulsification and aspiration (PEA) and intraocular lens (IOL) implantation. METHODS:Included in this study were 39 patients (53 eyes) with primary open-angle glaucoma with IOPs u...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(01)00322-7
更新日期:2001-05-01 00:00:00
abstract:PURPOSE:To establish a rat glaucoma model with chronic intraocular pressure (IOP) elevation induced by microbeads suspended in sodium sulfate-sodium hyaluronate. METHODS:Chronic elevation of IOP was induced unilaterally by injecting polystyrene microbeads, suspended in 4 % sodium sulfate and 3 % sodium hyaluronate, in...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0311-y
更新日期:2014-05-01 00:00:00
abstract:PURPOSE:To report a patient with agenesis of both internal carotid canals who presented with incomplete oculomotor palsy with pupil sparing. METHODS:The incomplete oculomotor palsy was followed clinically, and the precise anatomical relation of the aneurysm to the subarachnoid oculomotor nerve was investigated during ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-007-0481-y
更新日期:2007-11-01 00:00:00
abstract:PURPOSE:To evaluate dye retention in the fundus after indocyanine green (ICG)-assisted internal limiting membrane peeling. METHODS:Ten eyes with stage 3 or 4 nondiabetic idiopathic macular hole (MH group) and six eyes with diffuse diabetic macular edema (DM group) were studied. The fundus was examined with 780-nm infr...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-006-0325-1
更新日期:2006-07-01 00:00:00
abstract:PURPOSE:Topical mitomycin C (MMC) administration is reportedly effective in treating ocular surface neoplasms such as squamous cell carcinoma. We treated a case of ocular epithelial dysplasia that had spread too diffusely to be completely removed. We examined the ultrastructure of and c-met (hepatocyte growth factor re...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00270-7
更新日期:2000-11-01 00:00:00
abstract:PURPOSE:To report on isolated central retinal artery occlusion (CRAO) as an initial presentation in two patients with undiagnosed paroxysmal nocturnal hemoglobinuria (PNH). METHODS:CRAO related to the aggravation of PNH was observed in 2 of 98 consecutive PNH patients for 10 years. Ocular and systemic manifestations w...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,收录出版
doi:10.1007/s10384-013-0252-x
更新日期:2013-09-01 00:00:00
abstract::Movement of fluorescein and fluorescein glucuronide, a fluorescent metabolite of fluorescein, across the isolated iris-ciliary body of the albino rabbit was determined under short-circuit conditions using a modified Ussing's chamber. The permeabilities of this tissue to these dyes were calculated. The outward permeabi...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To clarify the redox states of the aqueous humor and serum in senile cataract patients. METHODS:We analyzed the reduced and oxidized albumin fractions in 58 patients. The patients had no detectable evidence of other eye or systemic diseases and were divided into two age groups (53-75 and 76-96 years). Analysis...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-010-0877-y
更新日期:2010-11-01 00:00:00
abstract::Purpose: We investigated the relationship between the progression of visual field defect and clinical factors in patients with normal-tension glaucoma (NTG).Subjects and Methods: Forty-eight eyes of 48 NTG patients undergoing more than 5-year follow-up were enrolled in this study. Their visual field defects ranged bet...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00334-8
更新日期:2001-01-01 00:00:00
abstract:PURPOSE:To identify any possible determinants in the development of uveitis in leprosy patients. METHODS:Human leukocyte antigen (HLA) class I and II antigen, and HLA class II genotypings were analyzed among Japanese leprosy patients. Ninety-three unrelated Japanese leprosy patients (46 patients with a history of uvei...
journal_title:Japanese journal of ophthalmology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0021-5155(98)00068-9
更新日期:1999-03-01 00:00:00
abstract:PURPOSE:To evaluate the effect of preservative-free (PF) tafluprost on diurnal variation of intraocular pressure (IOP) and ocular perfusion pressure (OPP), measured by use of home IOP and blood-pressure (BP) monitoring devices, for primary open angle glaucoma (POAG) patients. METHODS:Twenty-two eyes from 22 patients w...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-015-0413-1
更新日期:2016-01-01 00:00:00
abstract::Four patients with asteroid hyalosis who complained of seeing multiple spherical floaters were examined. Except for multiple brilliant particles in the gelatinous vitreous, no abnormalities were found by ophthalmoscopic examination. Asteroid bodies usually cause no visual symptoms in most patients. Therefore it is unu...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Although human immunodeficiency virus (HIV)-related ocular complexes are commonly observed in acquired immune deficiency syndrome (AIDS) patients, it has not been previously reported that HIV had already invaded the ocular tissues of HIV carriers. CASE:A 56-year-old woman was diagnosed as having rhegmatogen...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(00)00278-1
更新日期:2000-11-01 00:00:00
abstract::Management of intraocular tumors, specifically uveal melanoma, is a matter of controversy. The treatment of these ocular neoplasms varies from observation to enucleation, irradiation (cobalt plaque and proton-helium particle) and eye wall resection. Over the last 18 years, we have developed two techniques for surgical...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Distribution of microglia cells was studied in the normal retina of Wistar rats kept in a specific-pathogen-free condition, using a combined technique of light and electron microscopy with the weak silver carbonate staining method of del Rio-Hortega. Typical microglia cells were found mainly in the inner plexiform lay...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::Glycosaminoglycans (GAG) in pterygium tissues were analyzed and the results were compared with those in the normal human conjunctiva. A marked difference in the GAG composition was found: 78% of the total hexosamines of GAG from pterygium was glucosamine, whereas 98% of those of the conjunctiva was galactosamine. Pter...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract:PURPOSE:To assess whether the outcomes of toric intraocular lens (IOL) implantation in eyes with oblique astigmatism can be improved by direct measurements of posterior corneal astigmatism using anterior segment optical coherence tomography (AS-OCT) instead of by using anterior corneal measurements alone. STUDY DESIGN...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-021-00812-0
更新日期:2021-01-28 00:00:00
abstract::Prostaglandin D2 (PGD2) and 64E, a derivative of PGs, were tested for their capacity to suppress the growth of retinoblastoma in the nude mouse. Ten million cells of an established cell line of human retinoblastoma, Y-79 cells, were transferred subcutaneously into the nude mouse, and after the transferred cells became...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1987-01-01 00:00:00
abstract:PURPOSE:To report the reproducibility profile of optic nerve head parameters obtained by computer software-assisted fundus photoplanimetry. METHODS:Fundus photographs obtained during a population-based health survey (Sakurae Study) were planimetrically analyzed using newly developed computer software, CDSketch. The pa...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-013-0280-6
更新日期:2014-01-01 00:00:00
abstract::The effect of stimulus contrast changes on the binocular visual evoked response (VER) was investigated using pattern reversal VER, dichoptic stimulation, and Fast Fourier Transform. When the stimulus contrast was changed binocularly, the monocular components increased as the contrast increased. The binocular component...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract:PURPOSE:To compare the cytotoxicity of povidone-iodine solution (PVP-I) with that of polyvinyl alcohol-iodine solution (PAI) for ophthalmic use. METHODS:Cells of the human corneal epithelial cell line HCE-T were exposed to various dilutions of PVP-I or PAI, and the cytotoxicity of these two antiseptics was evaluated. ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-014-0348-y
更新日期:2014-11-01 00:00:00
abstract:PURPOSE:To evaluate dry eye symptoms, tear film function and ocular surface changes in patients with psoriasis. METHODS:The Dry Eye Questionnaire and ophthalmic examination including the Schirmer test, tear break-up time, corneal fluorescein test, meibomian gland obstruction and conjunctival impression cytology were a...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-012-0226-4
更新日期:2013-07-01 00:00:00
abstract:PURPOSE:To test the hypothesis that ischemia of the Schlemm's canal endothelium is involved in the control of intraocular pressure (IOP). METHODS:Experiments were conducted on pigeons. The course of the aqueous sinus and the aqueous sinus artery was determined by examining serial sections and the distribution of polym...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0021-5155(99)00017-9
更新日期:1999-07-01 00:00:00
abstract:PURPOSE:The aqueous humor (AH) contains numerous immunosuppressive molecules that contribute to the ocular immune privilege. Here, we mimic an inflammatory environment to analyze the inhibitory effects of the AH on lipopolysaccharide (LPS)-induced maturation of dendritic cells (DC). METHODS:Different concentrations of...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:10.1007/s10384-011-0060-0
更新日期:2011-09-01 00:00:00
abstract::A nationwide, multicenter study of typical retinitis pigmentosa was carried out in collaboration with 13 university hospitals throughout Japan. A total of 253 patients, 122 males and 131 females, with a wide range of ages (mean 48 years), were registered during a two-month period in 1989. Determination of inheritance ...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章,多中心研究
doi:
更新日期:1993-01-01 00:00:00
abstract::Experimental melanin-protein induced uveitis (EMIU) is a CD4 T cell-mediated disease involving the choroid and iris, but sparing the retina. The present study was designed to solubilize uveitogenic antigen from melanin granules without enzymatic digestion, and to investigate some of its elements by comparison with dif...
journal_title:Japanese journal of ophthalmology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00