Abstract：
PURPOSE:To establish a rat glaucoma model with chronic intraocular pressure (IOP) elevation induced by microbeads suspended in sodium sulfate-sodium hyaluronate. METHODS:Chronic elevation of IOP was induced unilaterally by injecting polystyrene microbeads, suspended in 4 % sodium sulfate and 3 % sodium hyaluronate, into the anterior chamber. The microbead suspension was injected through either the clear corneal (CC) or sclerocorneal (SC) tunnel. IOP changes were monitored up to 8 weeks after injection. The loss of retinal ganglion cells (RGCs) was assessed using fluorogold retrograde labeling of RGCs. RGC axons were evaluated by immunohistochemistry and immunoblotting. RESULTS:The resulting IOP elevation was maintained up to 3 weeks after the intracameral injection of microbeads through the CC route and up to 4 weeks after injection through the SC route. The density of RGCs was significantly reduced at 4 weeks after injection, with the SC route leading to more RGC loss than the CC route (p = 0.037). The neurofilament immunoreactivity and protein levels in the optic nerve were also significantly reduced at 4 weeks after injection. Some eyes in the SC route cohort received re-injection of the microbead suspension at 4 weeks after the initial injection, which led to an elevated IOP more than 8 weeks after the initial injection, and eventually a 27.5 % loss of RGC density compared with the control eyes. CONCLUSION:The intracameral injection of microbeads suspended in hyaluronate effectively produced chronic IOP elevation and subsequent RGC degeneration in rat eyes. The sclerocorneal tunnel approach yielded a longer period of IOP elevation than the clear corneal approach. Our modified microbead injection offers a reliable high-pressure glaucoma model.
journal_name
Jpn J Ophthalmoljournal_title
Japanese journal of ophthalmologyauthors
Matsumoto Y,Kanamori A,Nakamura M,Negi Adoi
10.1007/s10384-014-0311-ysubject
Has Abstractpub_date
2014-05-01 00:00:00pages
290-7issue
3eissn
0021-5155issn
1613-2246journal_volume
58pub_type
杂志文章abstract：:In an attempt to compare surgical peripheral iridectomy and laser iridotomy with regards to the long-term control of the intraocular pressure (IOP), analyses were carried out of the clinical records of 195 eyes of 149 primary angle-closure glaucoma patients who had undergone either peripheral iridectomy or laser irido...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:To determine whether the single-flash electroretinogram (ERG) can predict the postoperative outcome in diabetic cases where massive vitreous hemorrhage precludes fundus observation. METHODS:Eighty-five diabetic patients (105 eyes) who underwent vitrectomy due to dense vitreous hemorrhage were studied retrospec...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(03)00015-7
更新日期：2003-05-01 00:00:00
abstract：:Movement of fluorescein and fluorescein glucuronide, a fluorescent metabolite of fluorescein, across the isolated iris-ciliary body of the albino rabbit was determined under short-circuit conditions using a modified Ussing's chamber. The permeabilities of this tissue to these dyes were calculated. The outward permeabi...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：PURPOSE:To present and compare the long-term results of Dr. Tamcelik's previously described technique of Tenon advancement and duplication with the conventional Ahmed glaucoma valve (AGV) implantation technique in patients with refractory glaucoma. METHODS:This study was a multicenter, retrospective case series that i...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s10384-013-0249-5
更新日期：2013-07-01 00:00:00
abstract：:Localization of carbonic anhydrase (CA) was investigated in the rat eye by light and electron microscopy, and the inhibitory effects on CA distribution in the rat eye were compared between dorzolamide and acetazolamide. The cobalt sulfide histochemical method was used to study the CA localization. Acidic osmification ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1996-01-01 00:00:00
abstract：:Hematoxylin-eosin staining, the TUNEL method for in situ detection of the intranuclear DNA fragmentation, which indicates apoptosis, and electron microscopy were used to study the morphologic changes in specimens from the eyes of 8 patients with secondary glaucoma and 2 normal control eyes to evaluate our hypothesis t...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(97)00013-0
更新日期：1997-03-01 00:00:00
abstract：PURPOSE:We evaluated central retinal thickness (CRT) in diabetic macular edema (DME) using two different spectral-domain (SD) optical coherence tomography (OCT) instruments: the Cirrus and Spectralis OCTs. METHODS:CRT was measured in 63 eyes of 32 patients with DME using both instruments on the same day. RESULTS:CRT ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-011-0085-4
更新日期：2011-11-01 00:00:00
abstract：:The effect of stimulus contrast changes on the binocular visual evoked response (VER) was investigated using pattern reversal VER, dichoptic stimulation, and Fast Fourier Transform. When the stimulus contrast was changed binocularly, the monocular components increased as the contrast increased. The binocular component...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND:Ophthalmologists should be aware of the signs and symptoms of anthrax, although it is a rare disease in humans. We report our successful treatment of three patients with cutaneous lesions in the periorbital area. CASES:In this study, we report on the treatment of three female patients who were initially dia...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-003-0047-6
更新日期：2004-05-01 00:00:00
abstract：PURPOSE:From an early stage, retinitis pigmentosa (RP) patients suffer from night blindness which causes nocturnal mobility difficulties. We created a wearable visual aid that uses a high-performance see-through display, and added a high-sensitivity camera with a complementary metal-oxide-semiconductor sensor. Here, we...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-018-00644-5
更新日期：2019-03-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(03)00144-8
更新日期：2003-11-01 00:00:00
abstract：BACKGROUND:Interferon alpha-2b (IFN alpha 2b) was recently shown to be effective as an alternative therapy for conjunctival intraepithelial neoplasia (CIN). Herein, we report our successful management of CIN by the use of IFN alpha 2b. CASE:A 73-year-old woman presented with a complaint of irritation due to a tumorous...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(01)00494-4
更新日期：2002-03-01 00:00:00
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-011-0096-1
更新日期：2012-01-01 00:00:00
abstract：:Inflammation induced by systemic injection of endotoxin can be a good model for endogenous uveitis since ocular inflammation is induced without manipulating the eye. We carried out morphological and biochemical studies in Lewis rats to evaluate the breakdown of the blood-ocular barrier following injection of endotoxin...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:The head-mounted display (HMD) has produced conflict between visual and vestibular stimuli because the HMD image does not move with the head motion of the wearer. The HMD can show binocular parallax three-dimensional (3D) images, in which vergence and accommodation conflict. Thus, the HMD may affect the normal ...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(00)00289-6
更新日期：2001-01-01 00:00:00
abstract：PURPOSE:To elucidate clinical and angiographic features of retinal manifestations in cat scratch disease. METHODS:Clinical characteristics as well as fluorescein and indocyanine green (ICG) angiographic features were reviewed in 4 consecutive patients with retinal manifestations caused by serologically confirmed cat s...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(99)00195-1
更新日期：2000-03-01 00:00:00
abstract：:Long-term incubation of the lens of rainbow trout, a cold water fish, can be carried out at 0 degrees C, without altering its transparency. This is not possible in mammalian lenses. Rainbow trout lenses maintained their transparency with normal cation levels for 2.5 months in H10 medium, which is a simple balanced sal...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
abstract：PURPOSE:To evaluate the effects of topical latanoprost and timolol in combined therapy on retinal blood flow and tissue circulation in the optic nerve head (ONH) of the cynomolgus monkey. METHODS:Latanoprost (30 microL, 0.005%) was instilled once daily and timolol (30 microL, 0.5%) twice daily for 6 days into 1 eye, a...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(99)00217-8
更新日期：2000-05-01 00:00:00
abstract：PURPOSE:To study the effects of epigenetic events on corneal barrier functions, we examined tight junctions (TJs), the most important factor in the barrier function of the cornea, by using in vitro and in vivo corneal trauma models. METHODS:We examined alteration of TJ-associated gene expression and corneal barrier fu...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-008-0524-z
更新日期：2008-05-01 00:00:00
abstract：:Latanoprost (PhXA41, Xalatan) and isopropyl unoprostone (UF-021, unoprostone, Rescula) two new prostanoid derivatives, have been shown to reduce intraocular pressure (IOP) significantly in patients with glaucoma or ocular hypertension. This study was designed to compare the ocular hypotensive effects of latanoprost an...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(97)00128-7
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journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:A 10-year-old girl complained or poor vision in both eyes. The patient showed progeria, physical and mental retardation, sensorineural hearing loss, cutaneous photosensitivity, hyperopia, poor pupillary dilation, exotropia, salt-and-pepper fundi, nondetectable cone and rod electroretinographic (ERG) responses, cerebra...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：PURPOSE:To investigate the efficacy of intravitreal bevacizumab for the treatment of idiopathic macular telangiectasia (IMT). METHODS:Ten eyes of eight consecutive patients with IMT were studied. Four eyes had type 1, and six had type 2 IMT according to Yannuzzi's classification. All patients were treated with intravi...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-010-0810-4
更新日期：2010-07-01 00:00:00
abstract：PURPOSE:To determine the pharmacological response when dysiherbaine acts on cultured Müller cells, considering the glutamate receptor functions. Dysiherbaine is a new excitotoxic amino acid, which was recently isolated from the liquid extract of a certain sponge. METHODS:Retinas of adult rabbits were used to culture t...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(01)00488-9
更新日期：2002-03-01 00:00:00
abstract：PURPOSE:To investigate the changes in the electrolyte and protein concentrations in the vitreous of 3-week-old chicks with form-deprivation myopia (FDM). METHODS:FDM was induced in 2-day-old male white leghorn chicks by covering the left eye with a translucent plastic goggle and leaving the right eye uncovered to serv...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0021-5155(99)00177-x
更新日期：2000-01-01 00:00:00
abstract：PURPOSE:To measure choroidal thickness in healthy pregnant women using enhanced depth imaging optical coherence tomography (EDI-OCT) and compare the measurements with those of healthy non-pregnant women. METHODS:Choroidal thickness was measured at the fovea and at 3 mm superior, inferior, temporal, and nasal to the fo...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-013-0265-5
更新日期：2013-09-01 00:00:00
abstract：PURPOSE:The mortality rate of patients with retinoblastoma still varies in developing countries, and data for Asia and Taiwan are relatively scarce. In this retrospective study, we aimed to describe the survival characteristics and prognostic factors of 54 retinoblastoma cases diagnosed at the Taipei Veterans General H...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-005-0320-y
更新日期：2006-05-01 00:00:00
abstract：PURPOSE:Lymphoproliferative disorders (LPDs) can develop in patients treated with methotrexate (MTX) and usually respond well to MTX withdrawal. Mucosa-associated lymphoid tissue (MALT) lymphoma is a relatively rare type of MTX-LPD. The development of MTX-LPD in the orbit has not been previously described. We here repo...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-016-0439-z
更新日期：2016-05-01 00:00:00
abstract：:Diabetic retinopathy is a frequent cause of visual impairment in working-age adults (≥ 30 years) and in Japan is most commonly observed in those aged 50-69 years. Diabetic macular edema (DME) is one of the main causes of vision disturbance in diabetic retinopathy, which is a clinically significant microvascular compli...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1007/s10384-017-0537-6
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:To identify the cross-sectional characteristics of filtering blebs associated with intraocular pressure (IOP) control at 1 year after trabeculectomy with a square scleral flap and a fornix-based conjunctival flap. METHODS:A retrospective consecutive case series study was conducted. Eighty-four eyes of 79 patie...
journal_title：Japanese journal of ophthalmology
pub_type： 杂志文章
doi：10.1007/s10384-017-0504-2
更新日期：2017-05-01 00:00:00
