Abstract:
BACKGROUND:Genome-wide changes in DNA methylation are an epigenetic phenomenon that can lead to the development of disease. The study of global DNA methylation utilizes technology that requires both expensive equipment and highly specialized skill sets. METHODS:We have designed and developed an assay, CpGlobal, which is easy-to-use, does not utilize PCR, radioactivity and expensive equipment. CpGlobal utilizes methyl-sensitive restriction enzymes, HRP Neutravidin to detect the biotinylated nucleotides incorporated in an end-fill reaction and a luminometer to measure the chemiluminescence. The assay shows high accuracy and reproducibility in measuring global DNA methylation. Furthermore, CpGlobal correlates significantly with High Performance Capillary Electrophoresis (HPCE), a gold standard technology. We have applied the technology to understand the role of global DNA methylation in the natural history of lung cancer. World-wide, it is the leading cause of death attributed to any cancer. The survival rate is 15% over 5 years due to the lack of any clinical symptoms until the disease has progressed to a stage where cure is limited. RESULTS:Through the use of cell lines and paired normal/tumor samples from patients with non-small cell lung cancer (NSCLC) we show that global DNA hypomethylation is highly associated with the progression of the tumor. In addition, the results provide the first indication that the normal part of the lung from a cancer patient has already experienced a loss of methylation compared to a normal individual. CONCLUSION:By detecting these changes in global DNA methylation, CpGlobal may have a role as a barometer for the onset and development of lung cancer.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Anisowicz A,Huang H,Braunschweiger KI,Liu Z,Giese H,Wang H,Mamaev S,Olejnik J,Massion PP,Del Mastro RGdoi
10.1186/1471-2407-8-222subject
Has Abstractpub_date
2008-08-03 00:00:00pages
222issn
1471-2407pii
1471-2407-8-222journal_volume
8pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Recent randomized controlled trials comparing neoadjuvant chemoradiation plus surgery or perioperative chemotherapy plus surgery with surgery alone showed significant survival benefits for combined modality treatment of patients with localized esophageal adenocarcinoma. However, head-to-head comparisons of n...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-016-2564-y
更新日期:2016-07-19 00:00:00
abstract:BACKGROUND:Despite the increase in cancer incidence in the last years in Serbia, no nation-wide, population-based cancer epidemiology data have been reported. In this study cancer incidence and mortality rates for Serbia are presented using nation-wide data from two population-based cancer registries. These rates are a...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-18
更新日期:2013-01-15 00:00:00
abstract:BACKGROUND:Limited clinical data on real-world practice patterns are available for patients with metastatic/relapsed soft tissue sarcomas (STS). The primary objective of this study was to evaluate treatment patterns in patients with metastatic/relapsed STS following failure of prior chemotherapy by examining data colle...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1182-4
更新日期:2015-03-25 00:00:00
abstract:BACKGROUND:The 5-year overall survival rates for head and neck cancer (HNC) relies on distant metastasis. Importantly, the epithelial-mesenchymal transition (EMT) is believed to be an initial step of metastasis. However, the relationship of epigenetic with EMT formation is still unexplored in HNC. This study focuses on...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2468-x
更新日期:2016-07-08 00:00:00
abstract:BACKGROUND:We analyzed the changes in permeability of endothelial cell layers after photon irradiation, with a focus on the metalloproteases ADAM10 and ADAM17, and on VE-cadherin, components crucial for the integrity of endothelial intercellular junctions, and their roles in the transmigration of cancer cells through e...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6219-7
更新日期:2019-10-16 00:00:00
abstract:BACKGROUND:Interpretation of comparative health-related quality of life (HRQOL) studies following different prostate cancer treatments is often difficult due to differing patient ages. Furthermore, age-related changes can hardly be discriminated from therapy-related changes. The evaluation of age-and comorbidity-relate...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-9-296
更新日期:2009-08-24 00:00:00
abstract:BACKGROUND:To investigate the incidence of gastrointestinal stromal tumors (GISTs) in Taiwan and the impact of imatinib on the overall survival (OS) of GIST patients. METHODS:GISTs were identified from the Taiwan Cancer Registry (TCR) from 1998 to 2008. The age-adjusted incidence rates and the observed OS rates were c...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-102
更新日期:2014-02-18 00:00:00
abstract:BACKGROUND:Breast cancer risk, development, and treatment are influenced by genetic variation in certain genes, namely those involved in cell proliferation, tumor suppression, and drug metabolism. In turn, the relevance of the aforementioned genetic variation to cancer depends on the ethnic group in question, highlight...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6490-7
更新日期:2019-12-30 00:00:00
abstract:BACKGROUND:The prognosis for patients with recurrent glioblastoma is still poor with a median survival between 3 and 6 months. Reports about the application of carmustine (BCNU), one of the standard chemotherapeutic drugs in the treatment of newly diagnosed glioblastoma, in the recurrent situation are rare. METHODS:We...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-30
更新日期:2010-02-02 00:00:00
abstract:BACKGROUND:Mounting evidence demonstrates a causal role for S100 proteins in tumourigenesis and several S100 isoforms have shown utility as biomarkers of several types of cancer. The S100 family is comprised of 21 small isoforms, many of them implicated in important cellular functions such as proliferation, motility an...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1217-x
更新日期:2015-03-29 00:00:00
abstract:BACKGROUND:The cholesterol biosynthesis pathway is typically upregulated in breast cancer. The role of NAD(P)-dependent steroid dehydrogenase-like (NSDHL) gene, which is involved in cholesterol biosynthesis, in breast cancer remains unknown. This study aimed to uncover the role of NSDHL in the growth and metastasis of ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06840-2
更新日期:2020-05-04 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:BMC cancer
pub_type: 已发布勘误
doi:10.1186/s12885-020-07245-x
更新日期:2020-09-01 00:00:00
abstract:BACKGROUND:Pancreas ductal adenocarcinoma (PDAC) has the most dismal prognosis among all human cancers since it is highly resistant to chemotherapy, radiotherapy and immunotherapy. The anticipated consequence of all therapies is induction of tumor apoptosis. The highly resistance nature of PDACs to all therapies sugges...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4061-y
更新日期:2018-02-06 00:00:00
abstract:BACKGROUND:Axonal sensory peripheral neuropathy is the major dose-limiting side effect of paclitaxel.Omega-3 fatty acids have beneficial effects on neurological disorders from their effects on neurons cells and inhibition of the formation of proinflammatory cytokines involved in peripheral neuropathy. METHODS:This stu...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1186/1471-2407-12-355
更新日期:2012-08-15 00:00:00
abstract:BACKGROUND:Whether PET scan maximum standard uptake value (SUVmax) could differentiate luminal A from luminal B and help predict the survival of metastatic breast cancer (MBC) patients with luminal subtype is still unknown and need to be investigated. METHODS:305 MBC patients with luminal subtypes were screened with P...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-42
更新日期:2013-01-31 00:00:00
abstract:BACKGROUND:HAX-1 has been described as a protein potentially involved in carcinogenesis and especially metastasis. Its involvement in regulation of apoptosis and cell migration along with some data indicating its overexpression in cancer cell lines and tumors suggests that HAX-1 may play a role in neoplastic transforma...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-10-76
更新日期:2010-03-02 00:00:00
abstract:BACKGROUND:To determine the effect of surgery on the survival status of patients with locally advanced cervical cancer after radiotherapy/chemoradiotherapy. METHODS:PubMed, Web of Science, ProQuest and Medline were searched using the key words "cervical cancer", "locally advanced disease", "radiotherapy" and "surgery ...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析
doi:10.1186/s12885-018-4232-x
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:E2F1 transcription factor plays a vital role in the regulation of diverse cellular processes including cell proliferation, apoptosis, invasion and metastasis. E2F1 overexpression has been demonstrated in small cell lung cancer (SCLC), and extensive metastasis in early phase is the most important feature of S...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-276
更新日期:2014-04-22 00:00:00
abstract:BACKGROUND:Malignant Pleural Mesothelioma (MPM) is an aggressive tumor arising from mesothelial cells lining the pleural cavities characterized by resistance to standard therapies. Most of the molecular steps responsible for pleural transformation remain unclear; however, several growth factor signaling cascades are kn...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1363-1
更新日期:2015-05-08 00:00:00
abstract:BACKGROUND:Immune checkpoint inhibitors (ICIs) were approved to have a significant antitumor activity in various tumor types. In practice, some patients do not seem to benefit from ICIs but rather to have accelerating disease. The aim of this study was to evaluate hyperprogression in patients with malignant tumors of d...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5921-9
更新日期:2019-07-17 00:00:00
abstract:BACKGROUND:Whether perioperative blood transfusions (PBTs) adversely influence oncological outcomes for intrahepatic cholangiocarcinoma (ICC) patients after curative resection remains undetermined. METHODS:Of the 605 patients who underwent curative liver resection for ICC between 2000 and 2012, 93 received PBT. We con...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3745-z
更新日期:2017-11-14 00:00:00
abstract:BACKGROUND:Continued smoking after cancer adversely affects quality of life and survival, but one fifth of cancer survivors still smoke. Despite its demands, cancer presents an opportunity for positive behaviour change. Smoking often occurs in social groups, therefore interventions which target families and individuals...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3344-z
更新日期:2017-05-19 00:00:00
abstract:BACKGROUND:The objective of this study is to investigate the role and experience of early stage non-small cell lung cancer (NSCLC) patient in decision making process concerning treatment selection in the current clinical practice. METHODS:Stage I-II NSCLC patients (surgery 55 patients, SBRT 29 patients, median age 68)...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-3986-5
更新日期:2018-01-15 00:00:00
abstract:BACKGROUND:Angiogenesis is generally involved during the cancer development and hematogenous metastasis. Vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) are considered to have an important role in tumor-associated angiogenesis. However, the effects of simultaneously targeting on VE...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2834-8
更新日期:2016-10-12 00:00:00
abstract:BACKGROUND:The molecular chaperone, heat shock protein 90 (Hsp90) has been shown to be overexpressed in a number of cancers, including prostate cancer, making it an important target for drug discovery. Unfortunately, results with N-terminal inhibitors from initial clinical trials have been disappointing, as toxicity an...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-468
更新日期:2011-10-31 00:00:00
abstract:BACKGROUND:Several studies show that the regulatory impact of microRNAs (miRNAs) is an essential contribution to the pathogenesis of colorectal cancer (CRC). The expression levels of diverse miRNAs are associated with specific clinical diagnoses and prognoses of CRC. However, this association reveals very little action...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1437-0
更新日期:2015-06-18 00:00:00
abstract:BACKGROUND:The PTEN tumour suppressor gene and PIK3CA proto-oncogene encode proteins which contribute to regulation and propagation of signal transduction through the PI3K/AKT signalling pathway. This study investigates the prevalence of loss of PTEN expression and mutations in both PTEN and PIK3CA in colorectal cancer...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-123
更新日期:2011-04-07 00:00:00
abstract:BACKGROUND:Leukemia is different from solid tumor by harboring genetic rearrangements that predict prognosis and guide treatment strategy. PML-RARA, RUNX1-RUNX1T1, and KMT2A-rearrangement are common genetic rearrangements that drive the development of acute myeloid leukemia (AML). By contrast, rare genetic rearrangemen...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4396-4
更新日期:2018-04-23 00:00:00
abstract:BACKGROUND:Limited studies examine the immune landscape in Esophageal Adenocarcinoma (EAC). We aim to identify novel associations, which may inform immunotherapy treatment stratification. METHODS:Three hundred twenty-nine EAC cases were available in Tissue Microarrays (TMA) format. A discovery cohort of 166 EAC cases ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06987-y
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND:The transient receptor potential, subfamily V, member 6 (TRPV6) is a Ca(2+) selective cation channel. Several studies have shown that TRPV6 transcripts are expressed in locally advanced prostatic adenocarcinoma, metastatic and androgen-insensitive prostatic lesions but are undetectable in healthy prostate ti...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-9-380
更新日期:2009-10-26 00:00:00