In vitro antifungal activities of amphotericin B in combination with acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia.

Abstract:

:This study was undertaken to investigate the synergistic interaction between amphotericin B (AmB) and acteoside, isolated from the aerial parts of the shrub Colebrookea oppositifolia (Lamiaceae). Acteoside alone exhibited no intrinsic antifungal activity but showed a potent synergism in combination with AmB against selected pathogenic species, with fractional inhibitory concentration indices in the range of 0.0312-0.1562. The combination of acteoside at 3.12 and 12.5 µg ml(-1) with subinhibitory concentrations of AmB resulted in a potent fungicidal effect and also exhibited a significantly extended post-antifungal effect. Furthermore, the combination also reduced the minimum biofilm reduction concentration values of AmB (2-16-fold) in preformed biofilms of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. There was decreased viability of the cells, increased uptake of propidium iodide and enhanced leakage of 260 nm-absorbing material by Candida albicans cells when exposed to AmB in the presence of acteoside. The reason for potentiation is likely to be that the subinhibitory concentrations of AmB facilitated the uptake of acteoside, which resulted in increased killing of the fungal cells. Administration of acteoside in mice at up to 2000 mg (kg body weight)(-1) by the intraperitoneal or oral route produced no overt toxicity. The data presented here support synergism between acteoside and AmB, and it is therefore proposed that a prospective new management strategy for therapeutic application of this combination should be explored.

journal_name

J Med Microbiol

authors

Ali I,Sharma P,Suri KA,Satti NK,Dutt P,Afrin F,Khan IA

doi

10.1099/jmm.0.031906-0

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

1326-1336

issue

Pt 9

eissn

0022-2615

issn

1473-5644

journal_volume

60

pub_type

杂志文章
  • Mapping the genetic diversity within major clonal complexes of meticillin-resistant Staphylococcus aureus utilizing genome-wide fluorescent amplified fragment length polymorphism markers.

    abstract::The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049429-0

    authors: Desai D,Ellington MJ,Arnold C,Desai M

    更新日期:2012-12-01 00:00:00

  • Rapid differentiation of Prevotella intermedia and P. nigrescens by 16S rDNA PCR-RFLP.

    abstract::Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh iso...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-1-41

    authors: Milsom SE,Sprague SV,Dymock D,Weightman AJ,Wade WG

    更新日期:1996-01-01 00:00:00

  • Prevalence and characterization of quinolone resistance in Laribacter hongkongensis from grass carp and Chinese tiger frog.

    abstract::Laribacter hongkongensis is a food-borne bacterium associated with community-acquired gastroenteritis and diarrhoea. Quinolone resistance was recently reported in bacterial isolates from aquatic products, but the molecular mechanisms for resistance were still unknown. In this study, a total of 157 L. hongkongensis str...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.059451-0

    authors: Chen DQ,Yang L,Luo YT,Mao MJ,Lin YP,Wu AW

    更新日期:2013-10-01 00:00:00

  • Localisation of the mitogenic epitope of staphylococcal enterotoxin B.

    abstract::Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-3-155

    authors: Binek M,Newcomb JR,Rogers CM,Rogers TJ

    更新日期:1992-03-01 00:00:00

  • Resistance behaviour of inducible clindamycin-resistant staphylococci from clinical samples and aquatic environments.

    abstract::In this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin B (MLSB) isolated from clinical samples, sewage and river water was investigated. Inducible clindamycin resistance was tested using a D-test and macrodilution assays. Inducible cross-resistance...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.077081-0

    authors: Heß S,Gallert C

    更新日期:2014-11-01 00:00:00

  • Characteristics of toxicity and haemorrhagic toxin produced by Clostridium sporogenes in various animals and cultured cells.

    abstract::The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-4-270

    authors: Hara-Kudo Y,Ogura A,Noguchi Y,Kumagai S

    更新日期:1997-04-01 00:00:00

  • Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo).

    abstract::A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.038364-0

    authors: Radhouani H,Poeta P,Gonçalves A,Pacheco R,Sargo R,Igrejas G

    更新日期:2012-06-01 00:00:00

  • Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus isolated from a Malaysian teaching hospital.

    abstract::The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30 % to 40 % of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000387

    authors: Noordin A,Sapri HF,Mohamad Sani NA,Leong SK,Tan XE,Tan TL,Mohamad Zin N,Neoh HM,Hussin S

    更新日期:2016-12-01 00:00:00

  • The effects of ethyleneglycoltetra-acetic acid on bactericidal activity of human serum against Escherichia coli.

    abstract::The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-2-195

    authors: Roberts AP,Phillips R

    更新日期:1981-05-01 00:00:00

  • Liver abscess due to Neisseria sicca after repeated transcatheter arterial embolization.

    abstract::Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47192-0

    authors: Chung HC,Teng LJ,Hsueh PR

    更新日期:2007-11-01 00:00:00

  • Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010.

    abstract::Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034744-0

    authors: Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

    更新日期:2012-03-01 00:00:00

  • Candida auris: a comparison between planktonic and biofilm susceptibility to antifungal drugs.

    abstract::Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001036

    authors: Romera D,Aguilera-Correa JJ,Gadea I,Viñuela-Sandoval L,García-Rodríguez J,Esteban J

    更新日期:2019-09-01 00:00:00

  • Effect of intensive handwashing in the prevention of diarrhoeal illness among patients with AIDS: a randomized controlled study.

    abstract::Patients with AIDS frequently develop diarrhoeal illness. In this randomized, controlled study, 260 patients were screened for those who had not had diarrhoea in the preceding 3 months and who had received a stable highly active antiretroviral therapy regimen for at least 6 weeks prior to the study enrollment. A total...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1099/jmm.0.46867-0

    authors: Huang DB,Zhou J

    更新日期:2007-05-01 00:00:00

  • An invasive infection with an unusual spaB-possessing Erysipelothrix rhusiopathiae in a human.

    abstract::Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expres...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001252

    authors: Taguchi Y,Shiraiwa K,Ogawa Y,Oi T,Nakamura S,Okumura J,Shimoji Y

    更新日期:2020-10-01 00:00:00

  • Disseminated intravascular coagulation in rabbits: synergistic activity of meningococcal endotoxin and materials egested from leucocytes containing meningococci.

    abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-9-4-451

    authors: DeVoe IW,Gilka F

    更新日期:1976-11-01 00:00:00

  • Identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.

    abstract::Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46417-0

    authors: Colombo AV,Silva CM,Haffajee A,Colombo APV

    更新日期:2006-05-01 00:00:00

  • Motility of enterococci isolated from the urinary tract and from other sources.

    abstract::The identity and motility of enterococci isolated as urinary pathogens were compared with those of strains from other sources. The majority of isolates from all sources proved to be Streptococcus faecalis of S. faecium but eight of 117 urinary strains and seven of 127 non-urinary strains belonged to a group intermedia...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-1-127

    authors: Bransby RD,Grüneberg RN

    更新日期:1982-02-01 00:00:00

  • Ribotype differences between clinical and environmental isolates of Burkholderia pseudomallei.

    abstract::Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-7-565

    authors: Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

    更新日期:1997-07-01 00:00:00

  • Characterisation of cell-wall-derived polypeptide antigens from different species of Mycobacterium.

    abstract::Cell walls from different species of Mycobacterium were purified on a sucrose step gradient. The components derived from these preparations were characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by staining or by Western blotting. Surface-exposed polypeptide molecules were also iden...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-4-263

    authors: Babu S,Jain NK,Prasad G,Bhattacharya S,Bhattacharya A

    更新日期:1990-08-01 00:00:00

  • A real-time quantitative PCR assay for evaluating Clostridium difficile adherence to differentiated intestinal Caco-2 cells.

    abstract::Herein we describe a real-time quantitative PCR assay for evaluating the adherence of Clostridium difficile to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the C. difficile-specific triose-phosphate isomerase gene, is as reliable but less time-consuming than ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019752-0

    authors: Dingle T,Mulvey GL,Humphries RM,Armstrong GD

    更新日期:2010-08-01 00:00:00

  • Lesions produced by Clostridium butyricum strain CB 1002 in ligated intestinal loops in guinea pigs.

    abstract::Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-3-351

    authors: Popoff MR,Ravisse P

    更新日期:1985-06-01 00:00:00

  • Effect of EDTA on the resistance of clinical isolates of Acinetobacter baumannii to the bactericidal activity of normal human serum.

    abstract::Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-11-1047

    authors: García A,Solar H,González C,Zemelman R

    更新日期:2000-11-01 00:00:00

  • The third quorum-sensing system of Pseudomonas aeruginosa: Pseudomonas quinolone signal and the enigmatic PqsE protein.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen that produces several virulence factors such as lectin A, pyocyanin, elastase and rhamnolipids. These compounds are controlled transcriptionally by three quorum-sensing circuits, two based on the synthesis and detection of N-acyl-homoserine-lactone termed the Las and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.001116

    authors: García-Reyes S,Soberón-Chávez G,Cocotl-Yanez M

    更新日期:2020-01-01 00:00:00

  • Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices.

    abstract:PURPOSE:Candida biofilm infections are frequently linked to the use of biomaterials and are of clinical significance because they are commonly resistant to antifungals. Clioquinol is an antiseptic drug and is effective against multidrug-resistant Candida. We investigated the effect of clioquinol and two other 8-hydroxy...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000850

    authors: Pippi B,Machado GDRM,Bergamo VZ,Alves RJ,Andrade SF,Fuentefria AM

    更新日期:2018-11-01 00:00:00

  • Human Fusobacterium necrophorum strains have a leukotoxin gene and exhibit leukotoxic activity.

    abstract::Fusobacterium necrophorum, a Gram-negative anaerobe, causes a variety of necrotic infections in humans and animals. There are two subspecies: subsp. necrophorum and subsp. funduliforme. In cattle, subsp. necrophorum is more prevalent and production of leukotoxin is a major virulence factor. The leukotoxin operon (lktB...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47598-0

    authors: Tadepalli S,Stewart GC,Nagaraja TG,Narayanan SK

    更新日期:2008-02-01 00:00:00

  • Susceptibility to beta-lactam antibiotics of mutant strains of Xanthomonas maltophilia with high- and low-level constitutive expression of L1 and L2 beta-lactamases.

    abstract::Xanthomonas maltophilia produces two inducible beta-lactamases, L1 and L2, and resists the antimicrobial activity of beta-lactam antibiotics, including carbapenems. L1 is a zinc-metaloenzyme with carbapenemase activity; L2 is an unusual cephalosporinase. Mutant strains with high- and low-level constitutive expression ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-4-208

    authors: Akova M,Bonfiglio G,Livermore DM

    更新日期:1991-10-01 00:00:00

  • Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali, Indonesia.

    abstract::The purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete Brachyspira pilosicoli amongst Indonesians living in rural and urban settings on the island of Bali. Faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05415-0

    authors: Margawani KR,Robertson ID,Brooke CJ,Hampson DJ

    更新日期:2004-04-01 00:00:00

  • Evaluation of risk factors for colistin resistance among uropathogenic isolates of Escherichia coli and Klebsiella pneumoniae: a case-control study.

    abstract:INTRODUCTION:The last few years have seen the emergence of multi-drug resistant (MDR) Gram-negative infections, which are associated with high morbidity and mortality. The indiscriminate use of colistin has led to the development of resistance, which can be diagnosed effectively by broth microdilution. Studies from Ind...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000986

    authors: Kaza P,Mahindroo J,Veeraraghavan B,Mavuduru RS,Mohan B,Taneja N

    更新日期:2019-06-01 00:00:00

  • A particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci.

    abstract::The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-4-263

    authors: Pascu C,Hirmo S,Ljungh A,Wadström T

    更新日期:1996-10-01 00:00:00

  • Voriconazole susceptibility of yeasts isolated from the mouths of patients with advanced cancer.

    abstract::The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45720-0

    authors: Bagg J,Sweeney MP,Davies AN,Jackson MS,Brailsford S

    更新日期:2005-10-01 00:00:00