Abstract:
:The HIV-1 nucleocapsid protein (NC) is a nucleic acid chaperone, which remodels nucleic acid structures so that the most thermodynamically stable conformations are formed. This activity is essential for virus replication and has a critical role in mediating highly specific and efficient reverse transcription. NC's function in this process depends upon three properties: (1) ability to aggregate nucleic acids; (2) moderate duplex destabilization activity; and (3) rapid on-off binding kinetics. Here, we present a detailed molecular analysis of the individual events that occur during viral DNA synthesis and show how NC's properties are important for almost every step in the pathway. Finally, we also review biological aspects of reverse transcription during infection and the interplay between NC, reverse transcriptase, and human APOBEC3G, an HIV-1 restriction factor that inhibits reverse transcription and virus replication in the absence of the HIV-1 Vif protein.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Levin JG,Mitra M,Mascarenhas A,Musier-Forsyth Kdoi
10.4161/rna.7.6.14115subject
Has Abstractpub_date
2010-11-01 00:00:00pages
754-74issue
6eissn
1547-6286issn
1555-8584pii
14115journal_volume
7pub_type
杂志文章,评审相关文献
RNA Biology文献大全abstract::Myocyte enhancer factor 2c (MEF2C) is the MADS-box type transcription factor involved in the differentiation of cardiac and skeletal muscle and synaptic formation. Alternatively spliced transcripts of the MEF2C gene were proven to encode isoforms which exert distinct functions in transcriptional regulation. During the...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1017213
更新日期:2015-01-01 00:00:00
abstract::The genome of most prokaryotes gives rise to surprisingly complex transcriptomes, comprising not only protein-coding mRNAs, often organized as operons, but also harbors dozens or even hundreds of highly structured small regulatory RNAs and unexpectedly large levels of anti-sense transcripts. Comprehensive surveys of p...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.28647
更新日期:2014-01-01 00:00:00
abstract::Chemical modification of nucleobases plays an important role for the control of gene expression on different levels. That includes the modulation of translation by modified tRNA-bases or silencing and reactivation of genes by methylation and demethylation of cytosine in promoter regions. Especially dynamic methylation...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2017.1318241
更新日期:2017-09-02 00:00:00
abstract::The question of how cysteinyl-tRNA(Cys) is synthesized in organisms that lack a canonical cysteinyl-tRNA synthetase (CysRS) is an important open question in understanding protein synthesis. The prolyl-tRNA synthetase (ProRS) of wide ranging organisms has the ability to mis-activate cysteine without editing. This raise...
journal_title:RNA biology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::The GIR1 branching ribozyme constitutes a separate class of naturally occurring ribozymes. Most studies have been performed with the single GIR1 known from the myxomycete Didymium iridis whereas the large number of GIR1s found in the amoeboflagellate Naegleria has remained largely uncharacterized. Here, we investigate...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.6.16027
更新日期:2011-11-01 00:00:00
abstract::Viroids are the smallest autonomous infectious nucleic acids known today. They are non-coding, unencapsidated, circular RNAs with sizes ranging from 250 to 400 nucleotides and infect certain plants. These RNAs are transcribed by rolling-circle mechanisms in the plant host's nuclei (Pospiviroidae) or chloroplasts (Avsu...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.19810
更新日期:2012-06-01 00:00:00
abstract::Single-stranded oligonucleotides (ssOligos) are efficiently taken up by living cells without the use of transfection reagents. This phenomenon called 'gymnosis' enables the sequence-specific silencing of target genes in various types of cells. Several antisense ssOligos are used for the treatment of human diseases. Ho...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2017.1302641
更新日期:2017-11-02 00:00:00
abstract::Circular RNAs (circRNAs) are closely associated with the development of non-small cell lung cancer (NSCLC); however, it is still unclear whether circular RNA circ-LDLRAD3 participated in the regulation of NSCLC progression. In this study, we found that circ-LDLRAD3 was high-expressed and miR-137 was low-expressed in N...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1789819
更新日期:2020-12-01 00:00:00
abstract::In mammals, the genic disequilibrium between males (XY) and females (XX) is resolved through the inactivation of one of the X-chromosomes in females. X-chromosome inactivation (XCI) takes place in all mammalian species, but has mainly been studied in the mouse model where it was shown to be controlled by the interplay...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.25802
更新日期:2013-08-01 00:00:00
abstract::The universal genetic code is a translation table by which nucleic acid sequences can be interpreted as polypeptides with a wide range of biological functions. That information is used by aminoacyl-tRNA synthetases to translate the code. Moreover, amino acid properties dictate protein folding. We recently reported tha...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1112488
更新日期:2016-01-01 00:00:00
abstract::I am honored to have been asked to contribute to this memorial issue, although I cannot claim to have known Carl Woese well. Carl's insights and the discoveries that his research group made over the years certainly stimulated my own research program, and at several points early on, interactions with him were pivotal i...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.27799
更新日期:2014-01-01 00:00:00
abstract::Previous mRNA transcriptome studies of Euglena gracilis have shown that this organism possesses a large and diverse complement of protein coding genes; however, the study of non-coding RNA classes has been limited. The natural extensive fragmentation of the E. gracilis large subunit ribosomal RNA presents additional b...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2018.1526561
更新日期:2018-01-01 00:00:00
abstract::Wobble uridines (U34) are generally modified in all species. U34 modifications can be essential in metazoans but are not required for viability in fungi. In this review, we provide an overview on the types of modifications and how they affect the physico-chemical properties of wobble uridines. We describe the molecula...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2017.1295204
更新日期:2017-09-02 00:00:00
abstract::The tight gene expression regulation controls the development and pathogenesis of human malaria parasite Plasmodium falciparum throughout the complex life cycle. Recent studies have revealed the pervasive nascent transcripts in the genome of P. falciparum, suggesting the existence of a hidden transcriptome involved in...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1732032
更新日期:2020-06-01 00:00:00
abstract::Seryl-tRNA synthetase (SerRS) catalyzes the ligation of serine to the 3'-end of serine tRNA (tRNA(Ser)), which is typical of the type-2 tRNAs characterized by a long extra arm. The SerRSs are divided into two types, the archaeal/eukaryal and bacterial types. In this study, we solved the crystal structures of the SerRS...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.5.3.6876
更新日期:2008-07-01 00:00:00
abstract::RNase J is a prokaryotic 5'-3' exo/endoribonuclease that functions in mRNA decay and rRNA maturation. Here, we report a novel duplex unwinding activity of mpy-RNase J, an archaeal RNase J from Methanolobus psychrophilus, which enables it to degrade duplex RNAs with hairpins up to 40 bp when linking a 5' single-strande...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1777379
更新日期:2020-10-01 00:00:00
abstract::5-fluorouracil (5-FU) was isolated as an inhibitor of thymidylate synthase, which is important for DNA synthesis. The drug was later found to also affect the conserved 3'-5' exoribonuclease EXOSC10/Rrp6, a catalytic subunit of the RNA exosome that degrades and processes protein-coding and non-coding transcripts. Work ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1581596
更新日期:2019-06-01 00:00:00
abstract::Transcription termination delineates the 3' ends of transcripts, prevents otherwise runaway RNA polymerase (RNAP) from intruding into downstream genes and regulatory elements, and enables release of the RNAP for recycling. While other eukaryotic RNAPs require complex cis-signals and/or accessory factors to achieve the...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2015.1116677
更新日期:2016-01-01 00:00:00
abstract::The quaking viable (qk(v)) mice have attracted attention because of their characteristic tremor caused by their dysmyelination. In the central nervous system, qk(v) mice fail to develop mature myelinating oligodendrocytes and display uncompacted myelin. The genetic defect in the qk(v) mice prevents the proper expressi...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.2.2.1603
更新日期:2005-04-01 00:00:00
abstract::Editing by ADAR enzymes is essential for mammalian life. Still, knowledge of the spatio-temporal editing patterns in mammals is limited. By use of 454 amplicon sequencing we examined the editing status of 12 regionally extracted mRNAs from porcine developing brain encompassing a total of 64 putative ADAR editing sites...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.21082
更新日期:2012-08-01 00:00:00
abstract::We previously showed that miR-122 was frequently downregulated in hepatocellular carcinoma (HCC) and C/EBPα transactivated miR-122 expression. In this study, we found that Sp1 bound to the miR-122 promoter at two different sites. Interestingly, either inhibition or overexpression of Sp1 could decrease the miR-122 prom...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1673656
更新日期:2020-02-01 00:00:00
abstract::MicroRNAs (miRNAs) are key regulators of gene expression and play critical roles in modulating metabolism, development and physiology in animals and plants. miRNA levels are transcriptionally and post-transcriptionally controlled for their proper function. Recent studies have shown that RNA-binding proteins play impor...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.22740
更新日期:2012-12-01 00:00:00
abstract::Circular RNAs (circRNAs) constitute a large class of RNA species formed by the back-splicing of co-linear exons, often within protein-coding transcripts. Despite much progress in the field, it remains elusive whether the majority of circRNAs are merely aberrant splicing by-products with unknown functions, or their pro...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2016.1207036
更新日期:2016-09-01 00:00:00
abstract::The innate immune system is the first line of defense against microbial pathogens, but tight regulation of gene expression is necessary to prevent the detrimental effects of unrestrained activation. Although the functions of most long noncoding RNAs (lncRNAs; >200 nucleotides) are unknown, many have been shown to regu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29937
更新日期:2014-01-01 00:00:00
abstract::Glioma is the most malignant primary brain cancer which frequently occurred in adults. In recent years, long-non coding RNAs (lncRNAs) have been demonstrated to play pivotal roles in human cancers. However, the role of most lncRNAs in gliomagenesis has not been probed. Presently, through TCGA, a novel lncRNA LINC01198...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1755112
更新日期:2020-07-01 00:00:00
abstract::Population differences observed for complex traits may be attributed to the combined effect of socioeconomic, environmental, genetic and epigenetic factors. To better understand population differences in complex traits, genome-wide genetic and gene expression differences among ethnic populations have been studied. Her...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.8.4.16029
更新日期:2011-07-01 00:00:00
abstract::As a mental framework for the transition of self-replicating biological forms, the RNA world concept stipulates a dual function of RNAs as genetic substance and catalyst. The chaperoning function is found intrinsic to ribozymes involved in protein synthesis and tRNA maturation, enriching the primordial RNA world with ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1801199
更新日期:2021-01-01 00:00:00
abstract::Genuine La and La-related proteins group 7 (LARP7) bind to the non-coding RNAs transcribed by RNA polymerase III (RNAPIII), which end in UUU-3'OH. The La motif and RRM1 of these proteins (the La module) cooperate to bind the UUU-3'OH, protecting the RNA from degradation, while other domains may be important for RNA fo...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.23608
更新日期:2013-03-01 00:00:00
abstract::Deinococcus radiodurans is a highly stress resistant bacterium that encodes universal as well as lineage-specific factors involved in DNA transcription and repair. However, the effects of DNA lesions on RNA synthesis by D. radiodurans RNA polymerase (RNAP) have never been studied. We investigated the ability of this R...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1656027
更新日期:2019-12-01 00:00:00
abstract::RNA interference (RNAi) is a powerful tool for gene functional analysis of plant-parasitic nematodes (PPNs). RNAi involving soaking in a dsRNA solution and in planta methods is commonly applied in the study of gene function in PPNs. However, certain problems restrict the application of these methods. Therefore, more c...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1852779
更新日期:2020-12-14 00:00:00