Genome-wide methylation profile of nasal polyps: relation to aspirin hypersensitivity in asthmatics.


BACKGROUND:In addition to the dysregulation of arachidonic acid metabolism in aspirin-intolerant asthma (AIA), aspirin acetylsalicylic acid (ASA) exerts effects on inflammation and immunity; however, many of these effects are unknown. OBJECTIVE:The aim of the study was to evaluate the methylation status of whole genome in blood and polyp tissues with and without aspirin hypersensitivity. METHODS:Genome-wide DNA methylation levels in nasal polyps and peripheral blood cells were examined by microarray analysis using five subjects with AIA and four subjects with aspirin-tolerant asthma (ATA). RESULTS:In the nasal polyps of the patients with AIA, hypermethylation was detected at 332 loci in 296 genes, while hypomethylation was detected at 158 loci in 141 genes. Gene ontologic and pathway enrichment analyses revealed that genes involved in lymphocyte proliferation, cell proliferation, leukocyte activation, cytokine biosynthesis, cytokine secretion, immune responses, inflammation, and immunoglobulin binding were hypomethylated, while genes involved in ectoderm development, hemostasis, wound healing, calcium ion binding, and oxidoreductase activity were hypermethylated. In the arachidonate pathway, PGDS, ALOX5AP, and LTB4R were hypomethylated, whereas PTGES was hypermethylated. CONCLUSION:The nasal polyps of patients with AIA have characteristic methylation patterns affecting 337 genes. The genes and pathways identified in this study may be associated with the presence of aspirin hypersensitivity in asthmatics and are therefore attractive targets for future research.






Cheong HS,Park SM,Kim MO,Park JS,Lee JY,Byun JY,Park BL,Shin HD,Park CS




Has Abstract


2011-05-01 00:00:00














  • Clinical and immunological effects of oral immunotherapy with a standardized birch pollen extract.

    abstract::Oral immunotherapy (IT) was evaluated in a pilot study in two centres in children aged 8-15 years with allergic rhinoconjunctivitis. High doses (up to 20 X 10(6) BU monthly) of a defined freeze-dried birch pollen extract administered in enteric-coated gelatine capsules were given either daily for seven consecutive day...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Björkstén B,Möller C,Broberger U,Ahlstedt S,Dreborg S,Johansson SG,Juto P,Lanner A

    更新日期:1986-05-01 00:00:00

  • Matrix metalloproteinase-9: a novel biomarker for monitoring disease activity in patients with chronic urticaria patients?

    abstract:BACKGROUND:Matrix metalloproteinase (MMP)-9, an enzyme that contributes to inflammatory responses and subsequent tissue remodelling, has recently been suggested to be a good biomarker for monitoring disease activity in patients with chronic urticaria (CU). Here, we assessed whether total MMP-9 and/or active MMP-9 plasm...


    pub_type: 杂志文章


    authors: Altrichter S,Boodstein N,Maurer M

    更新日期:2009-04-01 00:00:00

  • Anaphylactic reaction caused by neoallergens in heated pecan nut.

    abstract::An atopic girl experienced an anaphylactic reaction after eating cookies containing pecan nuts. Investigations revealed that she had developed IgE antibodies exclusively specific against allergenic determinants present in aged or heated pecan nuts, but not in fresh pecans. These neoallergenic determinants were located...


    pub_type: 杂志文章


    authors: Malanin K,Lundberg M,Johansson SG

    更新日期:1995-12-01 00:00:00

  • Comparison of FEV1 and specific airway conductance in assessing airway response to occupational agents.

    abstract:BACKGROUND:There is theoretical evidence that specific airway conductance (SGaw) could be more reliable than forced expiratory volume in 1 s (FEV1) for assessing changes in airway calibre. We investigated the changes in FEV1 and SGaw when assessing bronchial responses to occupational agents. METHODS:SGaw and FEV1 were...


    pub_type: 杂志文章


    authors: Larbanois A,Delwiche JP,Jamart J,Vandenplas O

    更新日期:2003-12-01 00:00:00

  • Monitoring of mite Dermatophagoides farinae allergen-specific IgG and IgG subclass distribution in patients on immunotherapy.

    abstract::House dust mite D. farinae and Der f II-specific IgG and IgG subclass responses were evaluated in 32 adults with perennial rhinitis undergoing immunotherapy for 1 year by means of IgG-RAST and ELISA. The ELISA method, which is based on subclass-specific monoclonal antibodies, could detect 0.5-1.5 ng/ml of specific ant...


    pub_type: 杂志文章


    authors: Einarsson R,Dreborg S,Hammarström L,Löfkvist T,Smith CI,Svensson G

    更新日期:1992-04-01 00:00:00

  • Multiple roles of Bet v 1 ligands in allergen stabilization and modulation of endosomal protease activity.

    abstract:BACKGROUND:Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major birch pollen allergen. However, the molecular mechanisms of birch allergic sensitization, including the roles of Bet v 1 and other components of the birch pollen extract, remain incompletely understoo...


    pub_type: 杂志文章


    authors: Soh WT,Aglas L,Mueller GA,Gilles S,Weiss R,Scheiblhofer S,Huber S,Scheidt T,Thompson PM,Briza P,London RE,Traidl-Hoffmann C,Cabrele C,Brandstetter H,Ferreira F

    更新日期:2019-12-01 00:00:00

  • Detection of differentiation- and activation-linked cell surface antigens on cultured mast cell progenitors.

    abstract:BACKGROUND:Mast cells (MC) are multifunctional effector cells of the immune system. They derive from uncommitted CD34(+) hemopoietic progenitor cells (HPC). Depending on the stage of maturation and the environment, MC variably express differentiation- and activation-linked antigens. Little is known, however, about the ...


    pub_type: 杂志文章


    authors: Schernthaner GH,Hauswirth AW,Baghestanian M,Agis H,Ghannadan M,Worda C,Krauth MT,Printz D,Fritsch G,Sperr WR,Valent P

    更新日期:2005-10-01 00:00:00

  • Full automation in allergy testing: measurement of specific IgE by the ENEA System.

    abstract:UNLABELLED:We evaluated the ENEA System, a fully automated instrument for the measurement of specific IgE antibodies. The instrument dispenses sera and reagents, incubates, washes, and reads and prints results automatically. The "core" of the instrument is the reactive unit called ACE (Allergy Chamber Enzymatic), which...


    pub_type: 杂志文章


    authors: Plebani M,Faggian D,Borghesan F

    更新日期:1995-03-01 00:00:00

  • Bee moth (Galleria mellonella) allergic reactions are caused by several thermolabile antigens.

    abstract:BACKGROUND:Exposure and contact with bee moth (Galleria mellonella) larvae (Gm) can cause an allergic reaction both in anglers and breeders. We described the case of an amateur fisherman who experienced an allergic reaction using Gm but not using heat-treated Gm (h-Gm) (mummies). The aim of this study was to demonstrat...


    pub_type: 杂志文章


    authors: Villalta D,Martelli P,Mistrello G,Roncarolo D,Zanoni D

    更新日期:2004-09-01 00:00:00

  • Human IgE synthesis in vitro. Detection with monoclonal antibodies.

    abstract::Using monoclonal anti-human IgE antibodies recognizing the D epsilon 1 or D epsilon 2 epitope, we have developed a sandwich radioimmunoassay (RIA) to determine IgE in human cell cultures. With the help of this assay, various methods of measuring an actual de novo IgE synthesis were compared. It was necessary to subtra...


    pub_type: 杂志文章


    authors: Knutti-Müller JM,Stadler BM,Magnusson CM,de Weck L

    更新日期:1986-08-01 00:00:00

  • A double-blind controlled trial of elemental diet in severe, perennial asthma.

    abstract::In a double-blind controlled trial 41 hospitalized adults with severe, perennial asthma of unknown aetiology were allocated to either an antigen-free elemental diet (Vivasorb) or control diet, i.e. blended ordinary hospital food, for 2 weeks. At the time of entry into the trial all patients were in an active but stabl...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Høj L,Osterballe O,Bundgaard A,Weeke B,Weiss M

    更新日期:1981-05-01 00:00:00

  • Airway expression of calcitonin gene-related peptide in T-cell peptide-induced late asthmatic reactions in atopics.

    abstract:BACKGROUND:The mechanisms of late asthmatic reactions provoked in atopic asthmatics by allergen-derived T-cell peptide epitopes remain unclear. Previous studies showed no changes in airway eosinophils or mast cell products after peptide challenge. In the present study our aim was to measure calcitonin gene-related pept...


    pub_type: 杂志文章


    authors: Kay AB,Ali FR,Heaney LG,Benyahia F,Soh CP,Renz H,Lee TH,Larché M

    更新日期:2007-05-01 00:00:00

  • Mite-allergen levels in Norwegian homes.

    abstract::The scarce data published on mite sensitization and mite-allergen levels in Norway indicate that the mites may not be of the same importance in allergic disease in Norway as in countries such as the UK and the rest of Europe. The problem also involves the low allergen levels and small amounts of dust collected for all...


    pub_type: 杂志文章,评审


    authors: Gaarder PI

    更新日期:1998-01-01 00:00:00

  • Chitotriosidase inhibits allergic asthmatic airways via regulation of TGF-β expression and Foxp3+ Treg cells.

    abstract:BACKGROUND:Chitotriosidase (chitinase 1, Chit1), a major true chitinase in humans, is induced in childhood asthma and has been implicated in the pathogenesis of a variety of inflammatory and tissue remodeling responses. However, the role and the mechanisms that underlie these contributions to the diseases have not been...


    pub_type: 杂志文章


    authors: Hong JY,Kim M,Sol IS,Kim KW,Lee CM,Elias JA,Sohn MH,Lee CG

    更新日期:2018-08-01 00:00:00

  • Green, Yellow, and Red risk perception in everyday life - a communication tool.

    abstract:BACKGROUND:Adolescents have the highest risk for food allergy-related fatalities. Our main aim was to investigate the level of risk in everyday social situations as perceived by adolescents/young adults with peanut allergy, their families, and their friends. METHODS:The web-based 'Colours Of Risks' (COR) questionnaire...


    pub_type: 杂志文章


    authors: Stensgaard A,DunnGalvin A,Nielsen D,Munch M,Bindslev-Jensen C

    更新日期:2017-07-01 00:00:00

  • Epidermal Langerhans cells in patients with primary Sjögren's syndrome.

    abstract::IgG-binding to the surface of Langerhans cells (LC) is a feature of primary Sjögren's syndrome and might be associated with qualitative and quantitative defects in LC. Fifteen patients with primary Sjögren's syndrome and 15 healthy controls had a punch biopsy performed from clinically unaffected skin. LC were enumerat...


    pub_type: 杂志文章


    authors: Oxholm P,Oxholm A,Manthorpe R

    更新日期:1986-08-01 00:00:00

  • European birth cohort studies on asthma and atopic diseases: I. Comparison of study designs -- a GALEN initiative.

    abstract:BACKGROUND:The reasons for the rise in asthma and allergies remain unclear. To identify risk or protective factors, it is essential to carry out longitudinal epidemiological studies, preferably birth cohort studies. In Europe, several birth cohort studies on asthma and atopic diseases have been initiated over the last ...


    pub_type: 杂志文章,评审


    authors: Keil T,Kulig M,Simpson A,Custovic A,Wickman M,Kull I,Lødrup Carlsen KC,Carlsen KH,Smit HA,Wijga AH,Schmid S,Berg A,Bollrath C,Eller E,Bindslev-Jensen C,Halken S,Høst A,Heinrich J,Porta D,Forastiere F,Brunekreef B

    更新日期:2006-02-01 00:00:00

  • Protein S protects against allergic bronchial asthma by modulating Th1/Th2 balance.

    abstract:BACKGROUND:Bronchial asthma is a chronic disease characterized by inflammation, obstruction, and hyperresponsiveness of the airways. There is currently no curative therapy for asthma. Type 2 helper T cell response plays a critical role in the pathogenesis of the disease. Protein S is a glycoprotein endowed with anticoa...


    pub_type: 杂志文章


    authors: Asayama K,Kobayashi T,D'Alessandro-Gabazza CN,Toda M,Yasuma T,Fujimoto H,Okano T,Saiki H,Takeshita A,Fujiwara K,Fridman D'Alessandro V,Nishihama K,Totoki T,Inoue R,Takei Y,Gabazza EC

    更新日期:2020-09-01 00:00:00

  • Can food allergy be cured? What are the future prospects?

    abstract::Food allergies have become a significant heath burden as prevalence continues to rise, affecting 6%-13% of the global population. In the absence of drugs approved by regulatory agencies, the current standard of care remains avoidance of allergenic foods and management of acute allergic reactions with antihistamines an...


    pub_type: 杂志文章,评审


    authors: Sampath V,Sindher SB,Alvarez Pinzon AM,Nadeau KC

    更新日期:2020-06-01 00:00:00

  • Systemic contact dermatitis to copper-containing IUD.

    abstract::Although copper sulfate can cause systemic contact dermatitis, few such cases have been recorded among copper-releasing IUD users. Reported in this paper is a case of endometritis and urticaria-angioedema syndrome in a 32-year-old user of a copper IUD. Widespread urticaria, as well as angioedema of the eyelids and t...


    pub_type: 杂志文章


    authors: Purello D'Ambrosio F,Ricciardi L,Isola S,Gangemi S,Cilia M,Levanti C,Marcazzò A

    更新日期:1996-09-01 00:00:00

  • The value of SCORAD and beyond. Towards a standardized evaluation of severity?

    abstract::The clinical scoring systems of atopic dermatitis were analysed and compared. Some biological parameters that can correlate with the clinical score were also reviewed. After the definition of the disease based on validated clinical criteria, the second necessity was the availability of reliable severity scores to allo...


    pub_type: 杂志文章


    authors: Gelmetti C,Colonna C

    更新日期:2004-08-01 00:00:00

  • A comparative analysis of the cross-reactivity in the polcalcin family including Syr v 3, a new member from lilac pollen.

    abstract:BACKGROUND:Polcalcins are pollen-specific allergens with two EF-hand calcium-binding sites that exhibit strong cross-reactivity. Our objective was to isolate and express the cDNA coding of the EF-hand calcium-binding allergen from lilac pollen and to study cross-reactivity with other polcalcins from related and nonrela...


    pub_type: 杂志文章


    authors: Ledesma A,Barderas R,Westritschnig K,Quiralte J,Pascual CY,Valenta R,Villalba M,Rodríguez R

    更新日期:2006-04-01 00:00:00

  • A novel study design to investigate the early-life origins of asthma in children (SAGE study).

    abstract::This is a description of the Study of Asthma, Genes and the Environment (SAGE), a novel birth cohort created from provincial healthcare administrative records. It is a general population-based cohort, composed of children at high and low risk for asthma, living in urban and rural environments in Manitoba, Canada. The ...


    pub_type: 杂志文章


    authors: Kozyrskyj AL,HayGlass KT,Sandford AJ,Paré PD,Chan-Yeung M,Becker AB

    更新日期:2009-08-01 00:00:00

  • Ebastine in allergic rhinitis and chronic idiopathic urticaria.

    abstract::Histamine is a key mediator in the development of allergy symptoms, and oral H(1)-antihistamines are among the most widely used treatments for symptomatic relief in conditions such as allergic rhinitis and chronic urticaria. Ebastine is a second-generation antihistamine which has been shown to be an effective treatmen...


    pub_type: 杂志文章,评审


    authors: Sastre J

    更新日期:2008-12-01 00:00:00

  • Genetic and environmental factors in the changing incidence of allergy.

    abstract::Several studies have indicated that the incidence and prevalence of allergic diseases are increasing. Such data should not be regarded as mere statistical curiosities, but should be analysed to provide information on the factors contributing to allergy and its changing epidemiology. Extensive evidence has been accumul...


    pub_type: 杂志文章


    authors: Bonini S,Magrini L,Rotiroti G,Ronchetti MP,Onorati P

    更新日期:1994-01-01 00:00:00

  • Quantification of Blo t 5 in mite and dust extracts by two-site ELISA.

    abstract:BACKGROUND:Blomia tropicalis is an important mite species in the tropical and sub-tropical regions of the world. Blo t 5 is the major allergen with up to 70% sensitization rates in B. tropicalis allergic populations. METHODS:Mice were immunized intramuscularly with naked plasmid DNA encoding Blo t 5 gene with in vivo ...


    pub_type: 杂志文章


    authors: Yi FC,Lee BW,Cheong N,Chua KY

    更新日期:2005-01-01 00:00:00

  • Lolium perenne peptide immunotherapy is well tolerated and elicits a protective B-cell response in seasonal allergic rhinitis patients.

    abstract:BACKGROUND:Systemic allergic reactions are a risk for allergen immunotherapy that utilizes intact allergen preparations. We evaluated the safety, efficacy and immune mechanisms of short-course treatment with adjuvant-free Lolium perenne peptides (LPP) following a 6-week dose-escalation protocol. METHODS:In a prospecti...


    pub_type: 杂志文章


    authors: Mösges R,Koch AF,Raskopf E,Singh J,Shah-Hosseini K,Astvatsatourov A,Hauswald B,Yarin Y,Corazza F,Haazen L,Pirotton S,Allekotte S,Zadoyan G,Legon T,Durham SR,Shamji MH

    更新日期:2018-06-01 00:00:00

  • The effect of immediate-hypersensitivity reactions on the level of SLPI, granulocyte elastase, alpha1-antitrypsin, and albumin in nasal secretions, by the method of unilateral antigen challenge.

    abstract:BACKGROUND:The aim of this paper was to investigate the role of SLPI in patients with allergic rhinitis. From this point of view, we also examined leukocyte elastase, alpha1-antitrypsin, and albumin. SLPI is an inhibitor of serine proteases such as leukocyte elastase, cathepsin G, and mast-cell chymase. Since chymase i...


    pub_type: 杂志文章


    authors: Westin U,Lundberg E,Wihl JA,Ohlsson K

    更新日期:1999-08-01 00:00:00

  • Mutational spectrum and phenotypes in Danish families with hereditary angioedema because of C1 inhibitor deficiency.

    abstract:BACKGROUND:Hereditary angioedema (HAE), type I and II, is an autosomal dominant disease with deficiency of functional C1 inhibitor protein causing episodic swellings of skin, mucosa and viscera. HAE is a genetically heterogeneous disease with more than 200 different mutations in the SERPING1 gene. A genotype-phenotype ...


    pub_type: 杂志文章


    authors: Bygum A,Fagerberg CR,Ponard D,Monnier N,Lunardi J,Drouet C

    更新日期:2011-01-01 00:00:00

  • Role of nonallergic hypersensitivity reactions in children with chronic urticaria.

    abstract:BACKGROUND:IgE-independent (pseudoallergic) reactions to food and food ingredients are common in a subgroup of adult patients with chronic urticaria, who have daily spontaneous occurrence of wheals. However, for children with chronic urticaria (duration longer than 6 weeks, no physical influence), no data on the import...


    pub_type: 临床试验,杂志文章


    authors: Ehlers I,Niggemann B,Binder C,Zuberbier T

    更新日期:1998-11-01 00:00:00