Enumeration of viable CD34(+) cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit.

abstract：BACKGROUND AIMS:Hepatic stellate cells (HSCs) are liver-resident mesenchymal cells involved in essential processes in the liver. However, knowledge concerning these cells in human livers is limited because of the lack of a simple isolation method. METHODS:We isolated fetal and adult human liver cells by immunomagnetic...

journal_title：Cytotherapy

authors： Patil PB,Joshi M,Kuna VK,Xu B,Johannesson L,Olausson M,Sumitran-Holgersson S

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of ...

journal_title：Cytotherapy

authors： Harting M,Jimenez F,Pati S,Baumgartner J,Cox C Jr

更新日期：2008-01-01 00:00:00

abstract：:For more than a decade, Ab conjugated to a radionuclide emitting particulate radiation has been used in the management of leukemia in an effort to deliver targeted doses of radiation to BM, spleen and other sites of disease, while sparing normal organs. This radioimmunotherapy (RIT) approach has been employed to achie...

journal_title：Cytotherapy

authors： Pagel JM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been used to minimize and repair radiation-induced normal tissue injury in the intestine, salivary gland, liver, skin, lungs and cardiac muscle. This study investigated the ability of adipose tissue-derived MSCs (aMSCs) to minimize and/or repair single dose radiatio...

journal_title：Cytotherapy

authors： Maria OM,Shalaby M,Syme A,Eliopoulos N,Muanza T

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND AIMS:CD1d-restricted invariant natural killer (iNK) T cells are rare regulatory T cells that may contribute to the immune-regulation in allogeneic stem cell transplantation (ASCT). Here, we sought to develop an effective strategy to expand human iNK T cells for use in cell therapy to prevent graft-versus-hos...

journal_title：Cytotherapy

authors： Trujillo-Ocampo A,Cho HW,Herrmann AC,Ruiz-Vazquez W,Thornton AB,He H,Li D,Qazilbash MA,Ma Q,Porcelli SA,Shpall EJ,Molldrem J,Im JS

更新日期：2018-08-01 00:00:00

abstract：BACKGROUND:The isolation of lymphocytes - and removal of platelets (PLTs) and red blood cells (RBCs) - from an initial blood sample prior to culture is a key enabling step for effective manufacture of cellular therapies. Unfortunately, currently available methods suffer from various drawbacks, including low cell recove...

journal_title：Cytotherapy

authors： Strachan BC,Xia H,Vörös E,Gifford SC,Shevkoplyas SS

更新日期：2019-02-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) suppress T-cell proliferation, especially after activation with inflammatory cytokines. We compared the dynamic action of unprimed and interferon (IFN)-γ plus tumor necrosis factor (TNF)-α-pretreated human bone marrow-derived MSCs on resting or activated T cells. METHOD...

journal_title：Cytotherapy

authors： Cuerquis J,Romieu-Mourez R,François M,Routy JP,Young YK,Zhao J,Eliopoulos N

更新日期：2014-02-01 00:00:00

abstract：:Regulatory T cells (Tregs) play a fundamental role in the maintenance of self-tolerance and immune homeostasis. Defects in Treg function and/or frequencies have been reported in multiple disease models. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting upper and lower motor neur...

journal_title：Cytotherapy

authors： Alsuliman A,Appel SH,Beers DR,Basar R,Shaim H,Kaur I,Zulovich J,Yvon E,Muftuoglu M,Imahashi N,Kondo K,Liu E,Shpall EJ,Rezvani K

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND AIMS:Intrauterine adhesion (IUA) is a common uterine cavity disease characterized by the unsatisfactory regeneration of damaged endometria. Recently, stem cell transplantation has been proposed to promote the recovery process. Here we investigated whether human amniotic mesenchymal stromal cells (hAMSCs), a ...

journal_title：Cytotherapy

authors： Gan L,Duan H,Xu Q,Tang YQ,Li JJ,Sun FQ,Wang S

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...

journal_title：Cytotherapy

authors： Štach M,Ptáčková P,Mucha M,Musil J,Klener P,Otáhal P

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND AIMS:Treatment of tendon-derived stem cells (TDSCs) with connective tissue growth factor (CTGF) and ascorbic acid promoted their tenogenic differentiation. We investigated the effects of TDSCs pre-treated with CTGF and ascorbic acid on tendon repair in a patellar tendon window injury rat model. METHODS:Gree...

journal_title：Cytotherapy

authors： Lui PP,Wong OT,Lee YW

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND AIMS:T cells can be redirected to reject cancer by retroviral transduction with a chimeric antigen receptor (CAR) or by administration of a bispecific T cell engager (BiTE). We demonstrate that transfection of T cells with messenger (m) RNA coding for CAR is an alternative strategy. METHODS:We describe the ...

journal_title：Cytotherapy

authors： Almåsbak H,Rian E,Hoel HJ,Pulè M,Wälchli S,Kvalheim G,Gaudernack G,Rasmussen AM

更新日期：2011-05-01 00:00:00

abstract：BACKGROUND AIMS:Periodontal tissue regeneration with the use of mesenchymal stromal cells (MSCs) has been regarded as a future cell-based therapy. However, low survival rates and the potential tumorigenicity of implanted MSCs could undermine the efficacy of cell-based therapy. The use of conditioned media from MSCs (MS...

journal_title：Cytotherapy

authors： Kawai T,Katagiri W,Osugi M,Sugimura Y,Hibi H,Ueda M

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vi...

journal_title：Cytotherapy

authors： Mok PL,Cheong SK,Leong CF,Othman A

更新日期：2008-01-01 00:00:00

abstract：:Despite improved prophylaxis and treatment, GvHD remains a major limitation to optimal allogeneic stem cell transplantation. Ex vivo selective depletion (SD) is a strategy to prevent GvHD, in which host-reactive donor lymphocytes are selectively eliminated from a PBSC allograft while useful donor immune function is pr...

journal_title：Cytotherapy

authors： Mielke S,Solomon SR,Barrett AJ

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND AIMS:Cell therapy is promising as an exploratory cardiovascular therapy. We have recently developed an investigational new drug named Stempeucel (bone marrow-derived allogeneic mesenchymal stromal cells) for patients with acute myocardial infarction (AMI) with ST-segment elevation. A phase I/II randomized, d...

journal_title：Cytotherapy

authors： Chullikana A,Majumdar AS,Gottipamula S,Krishnamurthy S,Kumar AS,Prakash VS,Gupta PK

更新日期：2015-03-01 00:00:00

abstract：BACKGROUND AIMS:The aim was to investigate the therapeutic effect of granulocyte-colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia. METHODS:The design was a randomized controlled trial. Fifteen patients wit...

journal_title：Cytotherapy

authors： Zafarghandi MR,Ravari H,Aghdami N,Namiri M,Moazzami K,Taghiabadi E,Fazel A,Pournasr B,Farrokhi A,Sharifian RA,Salimi J,Moini M,Baharvand H

更新日期：2010-10-01 00:00:00

abstract：BACKGROUND AIMS:Apoptosis of radiosensitive cells in the bone marrow and gut is a serious, at times life-threatening, complication arising from radiation exposure. METHODS:We investigated whether adoptive transfer of allogeneic bone marrow-derived mesenchymal stromal cells (MSC) could exert cytoprotective and life-spa...

journal_title：Cytotherapy

authors： François M,Birman E,Forner KA,Gaboury L,Galipeau J

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND AIMS:The therapeutic potential of human mesenchymal stromal cells (MSCs) has generated considerable interest in a wide variety of areas. MSC banking is feasible, but the optimal technique of cryopreservation remains to be determined. METHODS:To reduce dimethyl sulfoxide (DMSO) concentration in cryopreservat...

journal_title：Cytotherapy

authors： Briquet A,Halleux A,Lechanteur C,Beguin Y

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) are gaining in popularity as an experimental therapy for a number of conditions that often require expansion ex vivo prior to use. Data comparing clinical-grade MSC from various ages of donors are scant. We hypothesized that MSC from older donors may display differences i...

journal_title：Cytotherapy

authors： Lund TC,Kobs A,Blazar BR,Tolar J

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Platelet transfusion can be a life-saving procedure in different medical settings. Thus, there is an increasing demand for platelets, of which shelf-life is only 5 days. The efficient ex vivo biomanufacturing of platelets would allow overcoming the shortages of donated platelets. METHODS:We exploited a...

journal_title：Cytotherapy

authors： Hatami J,Andrade PZ,Alves de Matos AP,Djokovic D,Lilaia C,Ferreira FC,Cabral JM,da Silva CL

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...

journal_title：Cytotherapy

authors： Kumar G,Hara H,Long C,Shaikh H,Ayares D,Cooper DK,Ezzelarab M

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND AIMS:To assess the impacts of stem cell therapy on clinical outcomes in patients with non-ischemic cardiomyopathy (NICM). The effect of stem cell therapy on prognosis is unclear and controversial. METHODS:The authors performed a systematic review and meta-analysis of the effects of autologous stem cell tran...

journal_title：Cytotherapy

authors： Xia L,Zeng L,Pan J,Ding Y

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND AIMS:Behcet's disease (BD) is a chronic, multisystemic inflammatory disorder with arthritic, gastrointestinal, mucocutaneous, ocular, vascular and central nervous system involvement. It is well known that CD4(+) CD25(+) T-regulatory (Treg) cells prevent harmful immune responses to self- and non-self-antigens...

journal_title：Cytotherapy

authors： Shim J,Lee ES,Park S,Bang D,Sohn S

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND AIMS:Adoptive transfer of tumor-infiltrating lymphocytes (TIL) has proven effective in metastatic melanoma and should therefore be explored in other types of cancer. The aim of this study was to examine the feasibility of potentially expanding clinically relevant quantities of tumor-specific T-cell cultures ...

journal_title：Cytotherapy

authors： Junker N,Andersen MH,Wenandy L,Dombernowsky SL,Kiss K,Sørensen CH,Therkildsen MH,Von Buchwald C,Andersen E,Straten PT,Svane IM

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND:Umbilical cord blood (UCB) is an alternative graft source for hematopoietic stem cell transplantation and has been shown to give results comparable to transplantation with other stem cell sources. Donor lymphocyte infusion (DLI) is an effective treatment for relapsed malignancies after hematopoietic stem cel...

journal_title：Cytotherapy

authors： Berglund S,Gertow J,Uhlin M,Mattsson J

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow mononuclear cells (BM-MNCs) and bone marrow-derived mesenchymal stem stromal cells (BM-MSCs) could have therapeutic potential for numerous conditions, including ischemia-related injury. Cells transplanted intravascularly may become entrapped in the lungs, which potentially decreases their th...

journal_title：Cytotherapy

authors： Mäkelä T,Takalo R,Arvola O,Haapanen H,Yannopoulos F,Blanco R,Ahvenjärvi L,Kiviluoma K,Kerkelä E,Nystedt J,Juvonen T,Lehenkari P

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:Clinical studies have shown the efficacy of combination therapy for various malignancies. In this study, the characteristics, safety and feasibility of use of cascade-primed (CAPRI) cells for the combination treatment of non-small-cell lung cancer (NSCLC) were evaluated both in vitro and in vivo. METHODS:Si...

journal_title：Cytotherapy

authors： Li H,Zhang Z,Duan X,Maimela NR,Yang S,Zhao X,Huang J,Zhang Y

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND:Container integrity is critical for maintaining sterility of cryopreserved cellular therapy products. We investigated a series of catastrophic bag failures, first noticed in early 2001. METHODS:Process records were reviewed for all PBPC and lymphocyte products cryopreserved in bags from January 2000 through...

journal_title：Cytotherapy

authors： Khuu HM,Cowley H,David-Ocampo V,Carter CS,Kasten-Sportes C,Wayne AS,Solomon SR,Bishop MR,Childs RM,Read EJ

更新日期：2002-01-01 00:00:00

abstract：:Pulmonary arterial hypertension (PAH) is characterized by pulmonary arterial endothelial hyperproliferation and dysfunction. Restoration of endothelial function is a common goal of available treatments. In the present study, human adipose-derived mesenchymal stromal cells (ASCs) were co-cultured with monocrotaline pyr...

journal_title：Cytotherapy

authors： Wang P,Zhang C,Li J,Luo L,Zhang S,Dong F,Tang Z,Ni S

更新日期：2019-04-01 00:00:00