Neuropeptides to replace serum in cryopreservation of mesenchymal stromal cells?

abstract：BACKGROUND AIMS:This study evaluated the feasibility, safety and immunological effects of the intravenous administration of mesenchymal stromal cells (MSCs) from a related donor in patients with refractory aplastic anemia (AA). METHODS:A mean of 6 × 10(5)/kg (range, 5.0-7.1 × 10(5)) MSCs were injected intravenously to...

journal_title：Cytotherapy

authors： Xiao Y,Jiang ZJ,Pang Y,Li L,Gao Y,Xiao HW,Li YH,Zhang H,Liu Q

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND AIMS:Tissue engineering technology is a promising therapeutic strategy in peripheral nerve injury. Schwann cells (SCs) are deemed to be a vital component of cell-based nerve regeneration therapies. Many methods for producing SC-like cells derived from adipose-derived stromal cells (ADSCs) have been explored,...

journal_title：Cytotherapy

authors： Kang Y,Liu Y,Liu Z,Ren S,Xiong H,Chen J,Duscher D,Machens HG,Liu W,Guo G,Zhan P,Chen H,Chen Z

更新日期：2019-09-01 00:00:00

abstract：BACKGROUND AIMS:Behcet's disease (BD) is a chronic, multisystemic inflammatory disorder with arthritic, gastrointestinal, mucocutaneous, ocular, vascular and central nervous system involvement. It is well known that CD4(+) CD25(+) T-regulatory (Treg) cells prevent harmful immune responses to self- and non-self-antigens...

journal_title：Cytotherapy

authors： Shim J,Lee ES,Park S,Bang D,Sohn S

更新日期：2011-08-01 00:00:00

abstract：BACKGROUND AIMS:The purpose of this study was to observe the clinical effect and safety of umbilical cord mesenchymal stem cells (UC-MSCs) in treating spinal cord injury (SCI) by intrathecal injection. METHODS:From January 2008 to October 2010, we treated 22 patients with SCI with UC-MSCs by intrathecal injection; dos...

journal_title：Cytotherapy

authors： Liu J,Han D,Wang Z,Xue M,Zhu L,Yan H,Zheng X,Guo Z,Wang H

更新日期：2013-02-01 00:00:00

abstract：BACKGROUND AIMS:The aim was to investigate the therapeutic effect of granulocyte-colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia. METHODS:The design was a randomized controlled trial. Fifteen patients wit...

journal_title：Cytotherapy

authors： Zafarghandi MR,Ravari H,Aghdami N,Namiri M,Moazzami K,Taghiabadi E,Fazel A,Pournasr B,Farrokhi A,Sharifian RA,Salimi J,Moini M,Baharvand H

更新日期：2010-10-01 00:00:00

abstract：:Regulatory T cells (Tregs) play a fundamental role in the maintenance of self-tolerance and immune homeostasis. Defects in Treg function and/or frequencies have been reported in multiple disease models. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting upper and lower motor neur...

journal_title：Cytotherapy

authors： Alsuliman A,Appel SH,Beers DR,Basar R,Shaim H,Kaur I,Zulovich J,Yvon E,Muftuoglu M,Imahashi N,Kondo K,Liu E,Shpall EJ,Rezvani K

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND:Retroviral vectors are regularly used to transduce stem cells and their derivatives for experimental and therapeutic purposes. Because these vectors integrate semi-randomly into the cellular genome, analysis of integranated retroviral DNA/host cell DNA junctions (IHJ) facilitates clonality studies of engraft...

journal_title：Cytotherapy

authors： Pule MA,Rousseau A,Vera J,Heslop HE,Brenner MK,Vanin EF

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

journal_title：Cytotherapy

authors： Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM)-derived mesenchymal stromal cells (MSC) are promising candidate cells for the development of neuroregenerative therapies. We have previously introduced the pro-neural conversion of human MSC to neural stem cell-like cells (m-NSC) by culturing them in suspension culture under serum-free condi...

journal_title：Cytotherapy

authors： Habisch HJ,Fiedler J,Ludolph AC,Storch A,Brenner RE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been shown to be a promising candidate for tissue regeneration and cancer therapy. However, their therapeutic potential against chemotherapy-induced side-effects remains unclear. METHODS:We treated murine Lewis lung carcinoma (LLC) and xenograft human colon tumors w...

journal_title：Cytotherapy

authors： Di GH,Jiang S,Li FQ,Sun JZ,Wu CT,Hu X,Duan HF

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND AIMS:The beneficial effect of human (h) mesenchymal stromal cell (MSC) transplantation in a variety of cell-based intervention strategies is widely believed to be because of paracrine mechanisms. The modification of hMSC cytokine and growth-factor expression patterns were studied following exposure to lipopo...

journal_title：Cytotherapy

authors： Montzka K,Führmann T,Müller-Ehmsen J,Wöltje M,Brook GA

更新日期：2010-11-01 00:00:00

abstract：:Initially used as an alternative hematopoietic stem cell source for patients without a human leukocyte antigen-matched bone marrow or peripheral blood stem cell donor, unrelated cord blood (UCB) is now the preferred donor source when hematopoietic stem cell transplantation (HSCT) is used to treat patients with lysosom...

journal_title：Cytotherapy

authors： Aldenhoven M,Kurtzberg J

更新日期：2015-06-01 00:00:00

abstract：BACKGROUND:Intracoronary application of BM-derived cells for the treatment of acute myocardial infarction (AMI) is currently being studied intensively. Simultaneously, strict legal requirements surround the production of cells for clinical studies. Thus good manufacturing practice (GMP)-compliant collection and prepara...

journal_title：Cytotherapy

authors： Griesel C,Heuft HG,Herrmann D,Franke A,Ladas D,Stiehler N,Stucki A,Braun M,Wollert KC,Meyer GP,Drexler H,Hertenstein B,Ganser A,Rüdinger W,Arseniev L

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND AIMS:Multiple cell-therapy products require density separation as a part of manufacturing. The traditional method for Ficoll separation, layering cell suspensions over Ficoll in tubes, followed by centrifugation and collection of cells from the interface, is too cumbersome and poses too high a risk of contam...

journal_title：Cytotherapy

authors： Janssen WE,Ribickas A,Meyer LV,Smilee RC

更新日期：2010-05-01 00:00:00

abstract：BACKGROUND AIMS:Existing treatments have limited success in modifying the course of peripheral artery disease, which may eventually lead to limb-threatening ulcers and amputation. Cellular therapies have the potential to provide a new treatment option for this condition, but isolation of cells by conventional means has...

journal_title：Cytotherapy

authors： Koch JM,D'Souza SS,Schwahn DJ,Dixon I,Hacker TA

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:Adipose tissue contains a stromal vascular fraction that can be easily isolated and provides a rich source of adipose tissue-derived mesenchymal stem cells (ASC). These ASC are a potential source of cells for tissue engineering. We studied whether the yield and growth characteristics of ASC were affected by ...

journal_title：Cytotherapy

authors： Oedayrajsingh-Varma MJ,van Ham SM,Knippenberg M,Helder MN,Klein-Nulend J,Schouten TE,Ritt MJ,van Milligen FJ

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:The use of myeloma Ag-loaded mature DC vaccines, cryopreserved in single-use aliquots, is an attractive immunotherapeutic strategy. In this study we investigated the retention of phenotype, viability and potency of DC vaccines after freezing and thawing. METHODS:Plastic-adherent monocytes, derived from a st...

journal_title：Cytotherapy

authors： Szmania S,Yi Q,Cottler-Fox M,Rosen NA,Freeman J,Kordsmeier BJ,Moreno A,Shi J,Barlogie B,Tricot G,van Rhee F

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND AIMS:Umbilical cord blood transplantation (UCBT) is increasingly used to treat acute leukemias. UCB units are thawed and infused in their entirety at transplant, precluding later use as immunotherapy to prevent or treat leukemia relapse. METHODS:We developed a device that selectively thaws only 1 mL of the ...

journal_title：Cytotherapy

authors： Vasu S,Berg M,Davidson-Moncada J,Tian X,Cullis H,Childs RW

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) are gaining in popularity as an experimental therapy for a number of conditions that often require expansion ex vivo prior to use. Data comparing clinical-grade MSC from various ages of donors are scant. We hypothesized that MSC from older donors may display differences i...

journal_title：Cytotherapy

authors： Lund TC,Kobs A,Blazar BR,Tolar J

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Corticosteroids are the standard first-line treatment for acute graft-versus-host disease (aGVHD), but they are associated with many complications, and less than half of patients have a sustained response. METHODS:To improve outcomes, we performed a retrospective study to analyze the efficacy of the ad...

journal_title：Cytotherapy

authors： Sestili S,Eder S,Belhocine R,Dulery R,Battipaglia G,Brissot E,Mediavilla C,Banet A,van de Wyngaert Z,Paviglianiti A,Ledraa T,Bonin A,Mohty M,Malard F

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND AIMS:In recent years, bone marrow (BM)-derived mesenchymal stromal cells (MSC) have become a promising source for neuroregenerative therapies. We evaluated the trophic effects of neuroectodermally converted MSC (mNSC) on neural stem cells (NSC). METHODS:We quantified the expression of growth factors by mNSC...

journal_title：Cytotherapy

authors： Habisch HJ,Liebau S,Lenk T,Ludolph AC,Brenner R,Storch A

更新日期：2010-07-01 00:00:00

abstract：:Kidney disease has reached epidemic proportions and is associated with high mortality and morbidity rates. Stem cell-based therapy may effectively treat kidney damage by cell transplantation. The breakthrough discovery using a combination of four transcription factors to reprogram genetically somatic cells into induce...

journal_title：Cytotherapy

authors： Wang WW,Wang W,Jiang Y,Han GF,Lu S,Li G,Zhang J

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND:Multiple myeloma (MM) is a hematologic cancer caused by the abnormal expansion of plasma cells, but the exact mechanism underlying MM development is not completely known. Recently, multiple long noncoding RNAs (lncRNAs) were implicated in the regulation of MM development. METHODS:Samples from patients with ...

journal_title：Cytotherapy

authors： Deng M,Yuan H,Liu S,Hu Z,Xiao H

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND AIMS:Chimeric antigen receptors (CARs) designed for adoptive immunotherapy need to achieve two functions: antigen recognition and triggering of the lytic machinery of reprogrammed effector cells. Cytotoxic T cells have been engineered with FcγRIII (CD16) chimeric molecules to be redirected against malignant ...

journal_title：Cytotherapy

authors： D'Aloia MM,Caratelli S,Palumbo C,Battella S,Arriga R,Lauro D,Palmieri G,Sconocchia G,Alimandi M

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND AIMS:Dendritic cell (DC)-based therapies could be important strategies for lymphoma treatment. METHODS:AKR/J mice with Dalton's lymphoma were treated with recombinant interleukin-15 (rIL-15)-activated autologous DCs and pulsed with whole tumor cell lysates in the presence or absence of suboptimal doses of t...

journal_title：Cytotherapy

authors： Hira SK,Mondal I,Manna PP

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND AIMS:Enumeration of viable CD34(+) cells provides critical information for the bone marrow (BM) transplant physician. The single-platform ISHAGE protocol is the most reliable method currently available to quantitate accurately this important subset of cells. Previous studies have shown that 5 CD34(+) cells/m...

journal_title：Cytotherapy

authors： Sutherland DR,Nayyar R,Acton E,Giftakis A,Dean S,Mosiman VL

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:The types of proteins released from mesenchymal stromal cells (MSC) are still unclear. Our aim was to compare apoptosis scores and the expression of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), neural cell adhesion molecule (NCAM)-1,matrix metalloproteinase (MMP)-1A, tissue inhibito...

journal_title：Cytotherapy

authors： Satar B,Hidir Y,Serdar MA,Kucuktag Z,Ural AU,Avcu F,Safali M,Oguztuzun S

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND AIMS:Human mesenchymal stromal cells (hMSC) are a promising tool for future clinical application, but their use requires rapid cell expansion in media suitable for clinical use. Therefore, we tested the influence of several culture media on colony formation, population doubling (PD) time, cell cycle and surf...

journal_title：Cytotherapy

authors： Turnovcova K,Ruzickova K,Vanecek V,Sykova E,Jendelova P

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Hematopoietic progenitor stem cells (HPSC) are a specialized transfusion product used for transplantation. Microbial contamination may occur during harvest or subsequent manipulation of these cells. The same difficulties in ensuring a safe, sterile, final product are faced in the preparation of other cell-th...

journal_title：Cytotherapy

authors： Lowder JN,Whelton P

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) stromal cells, also referred to as mesenchymal stromal cells (MSC), can be expanded ex vivo and are able to differentiate along multiple lineages, including chondrocytes, osteoblasts and adipocytes. MSC are known to secrete a number of cytokines and regulatory molecules implicated in di...

journal_title：Cytotherapy

authors： Lazzarotto-Silva C,Binato R,Rocher BD,Costa JA,Pizzatti L,Bouzas LF,Abdelhay E

更新日期：2009-01-01 00:00:00