Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure.

abstract：BACKGROUND AIMS:Platelet transfusion can be a life-saving procedure in different medical settings. Thus, there is an increasing demand for platelets, of which shelf-life is only 5 days. The efficient ex vivo biomanufacturing of platelets would allow overcoming the shortages of donated platelets. METHODS:We exploited a...

journal_title：Cytotherapy

authors： Hatami J,Andrade PZ,Alves de Matos AP,Djokovic D,Lilaia C,Ferreira FC,Cabral JM,da Silva CL

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND AIMS:The authors aim to analyze the evidence in the literature regarding the efficacy and safety of mesenchymal stem cell (MSC) therapy in human subjects with traumatic spinal cord injury (SCI) and identify its potential role in the management of SCI. METHODS:The authors conducted independent and duplicate ...

journal_title：Cytotherapy

authors： Muthu S,Jeyaraman M,Gulati A,Arora A

更新日期：2020-11-09 00:00:00

abstract：BACKGROUND AIMS:Adipose-derived stem cells are easily accessed and have a relatively high density compared with other mesenchymal stromal cells. Isolation protocols of adipose-derived stem cells (ASC) rely on the cell's ability to adhere to tissue culture plastic overnight. It was evaluated whether the floating ASC fra...

journal_title：Cytotherapy

authors： Buschmann J,Gao S,Härter L,Hemmi S,Welti M,Werner CM,Calcagni M,Cinelli P,Wanner GA

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND AIMS:Previous studies have shown that nicotine increases endothelial progenitor cell (EPC) numbers and functional activity. However, the mechanisms by which nicotine increases EPC numbers and activity remain to be determined. Recent studies have demonstrated that EPC numbers and activity are associated with ...

journal_title：Cytotherapy

authors： Junhui Z,Xiaojing H,Binquan Z,Xudong X,Junzhu C,Guosheng F

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Cell therapy is promising as an exploratory cardiovascular therapy. We have recently developed an investigational new drug named Stempeucel (bone marrow-derived allogeneic mesenchymal stromal cells) for patients with acute myocardial infarction (AMI) with ST-segment elevation. A phase I/II randomized, d...

journal_title：Cytotherapy

authors： Chullikana A,Majumdar AS,Gottipamula S,Krishnamurthy S,Kumar AS,Prakash VS,Gupta PK

更新日期：2015-03-01 00:00:00

abstract：BACKGROUND AIMS:Tumor antigen-specific cytotoxic T lymphocytes (CTL) have been used in the treatment of human cancer, including leukemia. Several studies have established PR1 peptide, an HLA-A2.1-restricted peptide derived from proteinase 3 (P3), as a human leukemia-associated antigen. PR1-specific CTL elicited in vitr...

journal_title：Cytotherapy

authors： Ma Q,Wang C,Jones D,Quintanilla KE,Li D,Wang Y,Wieder ED,Clise-Dwyer K,Alatrash G,Mj Y,Munsell MF,Lu S,Qazilbash MH,Molldrem JJ

更新日期：2010-12-01 00:00:00

abstract：OBJECTIVE:The objective of this study was to investigate the effects of the intravenous transplantation of bone marrow mesenchymal stromal cells (BM-MSCs) on the repair of glomerular endothelia and angiogenesis in rats with chronic renal failure (CRF). Furthermore, the mechanism of BM-MSCs promoting angiogenesis was ex...

journal_title：Cytotherapy

authors： Jia X,Pan J,Li X,Li N,Han Y,Feng X,Cui J

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND AIMS:It is important to improve the migratory ability of dendritic cells (DCs) and to increase DC potency for successful DC-based cancer immunotherapy. The intracellular Ca(2+) signaling pathway has an important role on the regulation of DC migration. Our preliminary studies revealed that sarco/endoplasmic r...

journal_title：Cytotherapy

authors： Choi NR,Lee HJ,Jung SH,Hong CY,Vo MC,Hoang MD,Kim HJ,Lee JJ

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...

journal_title：Cytotherapy

authors： Xu XQ,Zweigerdt R,Soo SY,Ngoh ZX,Tham SC,Wang ST,Graichen R,Davidson B,Colman A,Sun W

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Cell therapy has been proposed as a salvage limb procedure in critical limb ischemia (CLI). In spite of the fact that clinical trials found some efficacy, the mechanism of action remains elusive. The objective of this study was to characterize two autologous cell therapy products (CTPs) obtained from patient...

journal_title：Cytotherapy

authors： Tournois C,Pignon B,Sevestre MA,Al-Rifai R,Creuza V,Poitevin G,François C,Nguyen P

更新日期：2017-02-01 00:00:00

abstract：BACKGROUND AIMS:Corticosteroids are the standard first-line treatment for acute graft-versus-host disease (aGVHD), but they are associated with many complications, and less than half of patients have a sustained response. METHODS:To improve outcomes, we performed a retrospective study to analyze the efficacy of the ad...

journal_title：Cytotherapy

authors： Sestili S,Eder S,Belhocine R,Dulery R,Battipaglia G,Brissot E,Mediavilla C,Banet A,van de Wyngaert Z,Paviglianiti A,Ledraa T,Bonin A,Mohty M,Malard F

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Very few tumoral Ags have yet been isolated in NHL B cells. It is nevertheless possible to use whole tumor cells as a source of tumor Ags. We describe the purification of large numbers of human NHL B cells directly from lymph node or spleen biopsies, and different preparations allowing their use in a clinica...

journal_title：Cytotherapy

authors： Lui G,Manches O,Chaperot L,Ducrot T,Molens JP,Sotto JJ,Bensa JC,Plumas J

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND AIMS:Intrauterine adhesion (IUA) is a common uterine cavity disease characterized by the unsatisfactory regeneration of damaged endometria. Recently, stem cell transplantation has been proposed to promote the recovery process. Here we investigated whether human amniotic mesenchymal stromal cells (hAMSCs), a ...

journal_title：Cytotherapy

authors： Gan L,Duan H,Xu Q,Tang YQ,Li JJ,Sun FQ,Wang S

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been shown to be a promising candidate for tissue regeneration and cancer therapy. However, their therapeutic potential against chemotherapy-induced side-effects remains unclear. METHODS:We treated murine Lewis lung carcinoma (LLC) and xenograft human colon tumors w...

journal_title：Cytotherapy

authors： Di GH,Jiang S,Li FQ,Sun JZ,Wu CT,Hu X,Duan HF

更新日期：2012-04-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to suppress T-cell proliferation and used to alleviate the symptoms of graft-versus-host disease (GVHD). MSCs are a mixed cell population and at this time there are no tools to isolate the cells responsible for the T-cell suppressio...

journal_title：Cytotherapy

authors： McClain-Caldwell I,Vitale-Cross L,Mayer B,Krepuska M,Boyajian M,Myneni V,Martin D,GENOMICS AND COMPUTATIONAL BIOLOGY CORE.,Marko K,Nemeth K,Mezey E

更新日期：2018-12-01 00:00:00

abstract：BACKGROUND AND AIMS:We recently demonstrated that injection of conditioned medium (CM) generated from cells of the mesenchymal region of human amniotic membrane (AMTCs) reduces bleomycin-induced lung fibrosis in mice, suggesting a crucial role of paracrine factor(s) secreted by AMTCs in these beneficial effects. We fur...

journal_title：Cytotherapy

authors： Cargnoni A,Piccinelli EC,Ressel L,Rossi D,Magatti M,Toschi I,Cesari V,Albertini M,Mazzola S,Parolini O

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND AIMS:Stem cell therapy for liver diseases has recently emerged as a promising alternative to liver transplantation. Eligible cells should have an appropriate immunophenotype. The aim of the present study was to define the immunological profile of two human liver-derived mesenchymal stromal cell populations, ...

journal_title：Cytotherapy

authors： Raicevic G,Najar M,Najimi M,El Taghdouini A,van Grunsven LA,Sokal E,Toungouz M

更新日期：2015-02-01 00:00:00

abstract：BACKGROUND AIMS:Allogeneic hematopoietic stem cell transplantation is curative for sickle cell disease, and the use of matched related donors, non-myeloablative conditioning and sirolimus immunosuppression results in stable mixed chimerism without graft-versus-host disease (GVHD). However, the time to terminate sirolim...

journal_title：Cytotherapy

authors： Raines LN,Hsieh MM,Nassehi T,Drysdale CM,Tisdale JF,Uchida N

更新日期：2019-12-01 00:00:00

abstract：BACKGROUND:Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process o...

journal_title：Cytotherapy

authors： Serikov VB,Popov BV,Kropotov AV,Tomilin NV

更新日期：2005-01-01 00:00:00

abstract：:Mesenchymal stem cells (MSC) are multipotent cells that differentiate into osteoblasts, myocytes, chondrocytes and adipocytes as well as insulin-producing cells. The mechanism underlying their in vivo differentiation is not clear and is thought to be caused by spontaneous cell fusion or factors present in the microenv...

journal_title：Cytotherapy

authors： Kode JA,Mukherjee S,Joglekar MV,Hardikar AA

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:CD1d-restricted invariant natural killer (iNK) T cells are rare regulatory T cells that may contribute to the immune-regulation in allogeneic stem cell transplantation (ASCT). Here, we sought to develop an effective strategy to expand human iNK T cells for use in cell therapy to prevent graft-versus-hos...

journal_title：Cytotherapy

authors： Trujillo-Ocampo A,Cho HW,Herrmann AC,Ruiz-Vazquez W,Thornton AB,He H,Li D,Qazilbash MA,Ma Q,Porcelli SA,Shpall EJ,Molldrem J,Im JS

更新日期：2018-08-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cell (MSC) transplantation has immerged as promising therapeutic approach to treat spinal cord injury (SCI). In this pilot study, we investigated the safety of intrathecal injection of autologous bone marrow-derived MSCs in nine patients with SCI. METHODS:Patients with complete SCI ...

journal_title：Cytotherapy

authors： Satti HS,Waheed A,Ahmed P,Ahmed K,Akram Z,Aziz T,Satti TM,Shahbaz N,Khan MA,Malik SA

更新日期：2016-04-01 00:00:00

abstract：BACKGROUND AIMS:The types of proteins released from mesenchymal stromal cells (MSC) are still unclear. Our aim was to compare apoptosis scores and the expression of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), neural cell adhesion molecule (NCAM)-1,matrix metalloproteinase (MMP)-1A, tissue inhibito...

journal_title：Cytotherapy

authors： Satar B,Hidir Y,Serdar MA,Kucuktag Z,Ural AU,Avcu F,Safali M,Oguztuzun S

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND AIMS:Although bone marrow c-kit(+) progenitor cells support myocardial regeneration, the cardiomyocyte differentiation potential of umbilical cord blood (UCB) c-kit(+) cells is unknown. METHODS:UCB mononuclear cells (MNCs) and c-kit(+) cells purified by use of immunomagnetic beads were used. Cardiomyocyte d...

journal_title：Cytotherapy

authors： Iachininoto MG,Capodimonti S,Podda MV,Valentini CG,Bianchi M,Leone AM,Teofili L,Leone G

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND:PBSC transplant provides 10 times more T cells than BMT However, the incidence and severity of acute GvHD is similar among recipients of both types of transplants. Studies in mouse models suggest that the similar clinical outcome in BMT and PBSCT is due to differences in the lymphokine profiles. METHODS:PBM...

journal_title：Cytotherapy

authors： Vasconcelos ZF,Santos BM,Costa ES,Lima M,Tabak DG,Bouzas LF,Azevedo WM,Barcinski MA,Bonomo A

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND AIMS:Pooled human platelet lysate (HPL) is becoming the new gold standard as supplement for ex vivo cell culture for clinical protocols. However, the risk of pathogen contamination of HPL increases with the platelet pool size. We hypothesized that hollow fiber anion exchange membrane chromatography using Qyu...

journal_title：Cytotherapy

authors： Kao YC,Bailey A,Samminger B,Tanimoto J,Burnouf T

更新日期：2016-07-01 00:00:00

abstract：:Allogeneic hematopoietic cell transplantation (HCT) is an important therapeutic option for a variety of malignant and non-malignant disorders (NMD). The use of umbilical cord blood transplantation (UCBT) has made HCT available to many more patients. The increased level of human leukocyte antigen disparity that can be ...

journal_title：Cytotherapy

authors： Milano F,Boelens JJ

更新日期：2015-06-01 00:00:00

abstract：BACKGROUND:Patients with ulcerative colitis (UC) have elevated/activated myeloid lineage leucocytes and may respond favorably to adsorptive granulocyte/monocyte apheresis (GMA). However, there are patients who respond well to GMA, and patients who do not benefit. Therefore, predictive factors of GMA efficacy need to be...

journal_title：Cytotherapy

authors： Yokoyama Y,Watanabe K,Ito H,Nishishita M,Sawada K,Okuyama Y,Okazaki K,Fujii H,Nakase H,Masuda T,Fukunaga K,Andoh A,Nakamura S

更新日期：2015-05-01 00:00:00

abstract：BACKGROUND:Mismatched family donor and unrelated donor BM transplants are associated with a high risk of acute GvHD. White T-cell depletion is the best method to reduce risk of acute GvHD, there was a reluctance to use T-cell depletion in the mismatched setting because of increased risk of rejection and relapse. Partia...

journal_title：Cytotherapy

authors： Gajewski JL,Nimer S,Saliba RM,Thomas M,Przepiorka D,Giralt S,von Besien K,Mehra R,Andersson B,Chan KW,Ippoliti C,Warkinten D,Feigs S,Territo M,Schiller G,Lebkowski J,Moseley AM,Lloyd K,von Hoeff M,Okarma T,Champli

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND AIMS:Periodontal tissue regeneration with the use of mesenchymal stromal cells (MSCs) has been regarded as a future cell-based therapy. However, low survival rates and the potential tumorigenicity of implanted MSCs could undermine the efficacy of cell-based therapy. The use of conditioned media from MSCs (MS...

journal_title：Cytotherapy

authors： Kawai T,Katagiri W,Osugi M,Sugimura Y,Hibi H,Ueda M

更新日期：2015-04-01 00:00:00