当前位置： SCI文献检索 > CYTOTHERAPY期刊下所有文献 > T lymphocytes engineered to express a CD16-chimeric antigen receptor redirect T-cell immune responses against immunoglobulin G-opsonized target cells.

T lymphocytes engineered to express a CD16-chimeric antigen receptor redirect T-cell immune responses against immunoglobulin G-opsonized target cells.

Abstract：

BACKGROUND AIMS:Chimeric antigen receptors (CARs) designed for adoptive immunotherapy need to achieve two functions: antigen recognition and triggering of the lytic machinery of reprogrammed effector cells. Cytotoxic T cells have been engineered with FcγRIII (CD16) chimeric molecules to be redirected against malignant cells by monoclonal antibodies (mAbs). These cells have been proven to mediate granule-dependent cellular cytotoxicity, but it is not clear whether they can also kill malignant cells by a granule-independent mechanism of cell cytotoxicity. METHODS:We engineered a CD16A-CAR equipped with the extracellular CD16A, the hinge spacer and the transmembrane region of CD8, and the ζ-chain of the T-cell receptor/CD3 complex in tandem with the CD28 co-stimulatory signal transducer module. The CD16A-CAR was expressed and functionally tested in the MD45 cell line, a murine T-cell hybridoma with a defective granular exocytosis pathway but capable of killing target cells by a Fas ligand-mediated lysis. RESULTS:Our results indicate that in vitro cross-linking of CD16A-CAR on MD45 cells by the Fc fragment of mAb opsonized tumor cells induced interleukin-2 release and granule-independent cellular cytotoxicity. CONCLUSIONS:We conclude that strategies aimed to implement the therapeutic functions of mAbs used in the clinic with T-dependent immune responses driven by engineered T cells expressing FcγR-CAR can boost the antitumor efficacy of mAbs used in the clinic.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

D'Aloia MM,Caratelli S,Palumbo C,Battella S,Arriga R,Lauro D,Palmieri G,Sconocchia G,Alimandi M

doi

10.1016/j.jcyt.2015.10.014

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

278-90

issue

2

eissn

1465-3249

issn

1477-2566

pii

S1465-3249(15)01119-6

journal_volume

18

pub_type

杂志文章

相关文献

CYTOTHERAPY文献大全
  • Recombinant expression of coagulation factor VIII in hepatic and non-hepatic cell lines stably transduced with third generation lentiviral vectors comprising the minimal factor VIII promoter.

    abstract：BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240701656053

    authors： Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

    更新日期：2007-01-01 00:00:00

  • Stem cell comparison: what can we learn clinically from unrelated cord blood transplantation as an alternative stem cell source?

    abstract：:Allogeneic hematopoietic cell transplantation (HCT) is an important therapeutic option for a variety of malignant and non-malignant disorders (NMD). The use of umbilical cord blood transplantation (UCBT) has made HCT available to many more patients. The increased level of human leukocyte antigen disparity that can be ...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2015.03.003

    authors： Milano F,Boelens JJ

    更新日期：2015-06-01 00:00:00

  • New allergies after cord blood transplantation.

    abstract：BACKGROUND AIMS:Umbilical cord blood transplantation (CBT) is an effective treatment for benign and malignant diseases. Late effects of CBT are not well described in the literature. In the present study, we present our experience of new-onset allergies in long-term survivors after CBT. METHODS:After an initial patient...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.05.018

    authors： Vaughan LA,Vu M,Sengsayadeth S,Lucid C,Clifton C,McCarty K,Hagaman D,Domm J,Kassim A,Chinratanalab W,Goodman S,Greer J,Frangoul H,Engelhardt BG,Jagasia M,Savani BN

    更新日期：2013-10-01 00:00:00

  • Current state of U.S. Food and Drug Administration regulation for cellular and gene therapy products: potential cures on the horizon.

    abstract：:Cellular & Gene Therapies (CGTs) are complex products, which have been key foci of the International Society for Cell & Gene Therapy (ISCT). For this ISCT North American Legal & Regulatory Affairs Committee review publication, CGTs include but are not limited to somatic cell-based therapies, pluripotent cell-derived c...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2019.04.002

    authors： Mendicino M,Fan Y,Griffin D,Gunter KC,Nichols K

    更新日期：2019-07-01 00:00:00

  • Human allogeneic AB0/Rh-identical umbilical cord blood cells in the treatment of juvenile patients with cerebral palsy.

    abstract：BACKGROUND AIMS:The term "cerebral palsy" (CP) encompasses many syndromes that emerge from brain damage at early stages of ontogenesis and manifest as the inability to retain a normal body position or perform controlled movements. Existing methods of CP treatment, including various rehabilitation strategies and surgica...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.02.010

    authors： Romanov YA,Tarakanov OP,Radaev SM,Dugina TN,Ryaskina SS,Darevskaya AN,Morozova YV,Khachatryan WA,Lebedev KE,Zotova NS,Burkova AS,Sukhikh GT,Smirnov VN

    更新日期：2015-07-01 00:00:00

  • Combined immunotherapy with whole tumor lysate-pulsed interleukin-15-activated dendritic cells and cucurbitacin I promotes strong CD8(+) T-cell responses and cures highly aggressive lymphoma.

    abstract：BACKGROUND AIMS:Dendritic cell (DC)-based therapies could be important strategies for lymphoma treatment. METHODS:AKR/J mice with Dalton's lymphoma were treated with recombinant interleukin-15 (rIL-15)-activated autologous DCs and pulsed with whole tumor cell lysates in the presence or absence of suboptimal doses of t...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.01.006

    authors： Hira SK,Mondal I,Manna PP

    更新日期：2015-05-01 00:00:00

  • Ex vivo expansion of mafosfamide-purged PBPC products.

    abstract：BACKGROUND:Multiple studies have demonstrated that 'purging' of autografts with 4-hydroperoxycyclophosphamide (4HC) or the related compound mafosfamide (Mf), to eradicate residual leukemia, produces the best results associated with autologous blood and marrow transplantation for AML. However, 4HC purging results in pro...

    journal_title：Cytotherapy

    pub_type： 临床试验,杂志文章

    doi：10.1080/14653240600905353

    authors： McNiece I,Civin C,Harrington J,Kellner J,Malehorn M,Turney J,Barber J,Jones R

    更新日期：2006-01-01 00:00:00

  • Developing a co-culture system for effective megakaryo/thrombopoiesis from umbilical cord blood hematopoietic stem/progenitor cells.

    abstract：BACKGROUND AIMS:Platelet transfusion can be a life-saving procedure in different medical settings. Thus, there is an increasing demand for platelets, of which shelf-life is only 5 days. The efficient ex vivo biomanufacturing of platelets would allow overcoming the shortages of donated platelets. METHODS:We exploited a...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.12.010

    authors： Hatami J,Andrade PZ,Alves de Matos AP,Djokovic D,Lilaia C,Ferreira FC,Cabral JM,da Silva CL

    更新日期：2015-04-01 00:00:00

  • Reprogramming of mouse renal tubular epithelial cells to induced pluripotent stem cells.

    abstract：:Kidney disease has reached epidemic proportions and is associated with high mortality and morbidity rates. Stem cell-based therapy may effectively treat kidney damage by cell transplantation. The breakthrough discovery using a combination of four transcription factors to reprogram genetically somatic cells into induce...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.01.008

    authors： Wang WW,Wang W,Jiang Y,Han GF,Lu S,Li G,Zhang J

    更新日期：2013-05-01 00:00:00

  • Proteomic analysis of human mesenchymal stromal cells derived from adipose tissue undergoing osteoblast differentiation.

    abstract：BACKGROUND AIMS:Stem cells derived from human adipose tissue (ASC) have the capacity for renewal, are easily obtained and have plasticity properties that allow them to differentiate into several cell types, including osteoblast cells. With the aim of understanding the issue of the osteogenic process and finding reliabl...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653240903580270

    authors： Giusta MS,Andrade H,Santos AV,Castanheira P,Lamana L,Pimenta AM,Goes AM

    更新日期：2010-07-01 00:00:00

  • Cryopreserved mesenchymal stromal cell treatment is safe and feasible for severe dilated ischemic cardiomyopathy.

    abstract：BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653240903313966

    authors： Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

    更新日期：2010-01-01 00:00:00

  • Decrease in circulating hematopoietic progenitor cells by trapping in the pulmonary circulation.

    abstract：BACKGROUND:When stem-cell grafts are infused into the venous circulation and stem/progenitor cells egress from BM, pulmonary capillary beds are the first microcirculation site that they encounter. This provides the potential for circulating progenitor cells to be trapped in the pulmonary circulation. METHODS:We compar...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/146532401317248063

    authors： Watanabe T,Kajiume T,Takaue Y,Kawano Y,Kanamaru S,Okamura S,Onishi T,Suzuya H,Nakagawa R,Kuroda Y,Talmadge JE

    更新日期：2001-01-01 00:00:00

  • Genomic and functional comparison of mesenchymal stromal cells prepared using two isolation methods.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been applied to patients in cell therapy for various diseases. Recently, we introduced a novel MSC separation filter device which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system compared with the conventional open density gradient ce...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.10.013

    authors： Otsuru S,Hofmann TJ,Raman P,Olson TS,Guess AJ,Dominici M,Horwitz EM

    更新日期：2015-03-01 00:00:00

  • IL-2-activated cord blood mononuclear cells.

    abstract：BACKGROUND:[corrected] Recent findings in cord blood (CB) research indicate the potential clinical usefulness of IL-2-activated CB in eradication of minimal malignant residual disease after hematopoietic stem cell transplantation. This feasible approach to immunotherapy merits further pre-clinical investigations using ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240500319184

    authors： Derzic S,Slone V,Sender L

    更新日期：2005-01-01 00:00:00

  • Protein profiling of anastomosed facial nerve treated with mesenchymal stromal cells.

    abstract：BACKGROUND AIMS:The types of proteins released from mesenchymal stromal cells (MSC) are still unclear. Our aim was to compare apoptosis scores and the expression of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), neural cell adhesion molecule (NCAM)-1,matrix metalloproteinase (MMP)-1A, tissue inhibito...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.651530

    authors： Satar B,Hidir Y,Serdar MA,Kucuktag Z,Ural AU,Avcu F,Safali M,Oguztuzun S

    更新日期：2012-05-01 00:00:00

  • Autologous mesenchymal stromal cell transplantation for spinal cord injury: A Phase I pilot study.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cell (MSC) transplantation has immerged as promising therapeutic approach to treat spinal cord injury (SCI). In this pilot study, we investigated the safety of intrathecal injection of autologous bone marrow-derived MSCs in nine patients with SCI. METHODS:Patients with complete SCI ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.01.004

    authors： Satti HS,Waheed A,Ahmed P,Ahmed K,Akram Z,Aziz T,Satti TM,Shahbaz N,Khan MA,Malik SA

    更新日期：2016-04-01 00:00:00

  • A robust, good manufacturing practice-compliant, clinical-scale procedure to generate regulatory T cells from patients with amyotrophic lateral sclerosis for adoptive cell therapy.

    abstract：:Regulatory T cells (Tregs) play a fundamental role in the maintenance of self-tolerance and immune homeostasis. Defects in Treg function and/or frequencies have been reported in multiple disease models. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting upper and lower motor neur...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.06.012

    authors： Alsuliman A,Appel SH,Beers DR,Basar R,Shaim H,Kaur I,Zulovich J,Yvon E,Muftuoglu M,Imahashi N,Kondo K,Liu E,Shpall EJ,Rezvani K

    更新日期：2016-10-01 00:00:00

  • In vitro expression of erythropoietin by transfected human mesenchymal stromal cells.

    abstract：BACKGROUND:Mesenchymal stromal cells (MSC) are pluripotent progenitor cells that can be found in human bone marrow (BM). These cells have low immunogenicity and could suppress alloreactive T-cell responses. In the current study, MSC were tested for their capacity to carry and deliver the erythropoietin (EPO) gene in vi...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240701816996

    authors： Mok PL,Cheong SK,Leong CF,Othman A

    更新日期：2008-01-01 00:00:00

  • Expansion in microcarrier-spinner cultures improves the chondrogenic potential of human early mesenchymal stromal cells.

    abstract：BACKGROUND AIMS:Cartilage tissue engineering with human mesenchymal stromal cells (hMSC) is promising for allogeneic cell therapy. To achieve large-scale hMSC propagation, scalable microcarrier-based cultures are preferred over conventional static cultures on tissue culture plastic. Yet it remains unclear how microcarr...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.03.293

    authors： Lin YM,Lim JF,Lee J,Choolani M,Chan JK,Reuveny S,Oh SK

    更新日期：2016-06-01 00:00:00

  • Expression and role of Toll-like receptors on human umbilical cord mesenchymal stromal cells.

    abstract：BACKGROUND AIMS:Toll-like receptors (TLRs) play an important role in innate and adaptive immunity by recognizing pathogen-associated molecular patterns (PAMPs). METHODS:In the present study, we investigated the expression and role of TLRs on human umbilical cord mesenchymal stromal cells (UC-MSCs). The proliferation, ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2012.12.001

    authors： Chen D,Ma F,Xu S,Yang S,Chen F,Rong L,Chi Y,Zhao Q,Lu S,Han Z,Pang A,Han Z

    更新日期：2013-04-01 00:00:00

  • Large-scale production and directed induction of functional dendritic cells ex vivo from serum-free expanded human hematopoietic stem cells.

    abstract：BACKGROUND:Dendritic cells (DCs) that are derived from hematopoietic stem cells (HSCs) are the most potent antigen-presenting cells and play a pivotal role in initiating the immune response. Hence, large-scale production and direct induction of functional DCs ex vivo from HSCs are crucial to HSC research and clinical p...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2019.04.059

    authors： Hsu SC,Lu LC,Chan KY,Huang CH,Cheng SL,Chan YS,Yang YS,Lai YT,Yao CL

    更新日期：2019-07-01 00:00:00

  • Neuropeptides to replace serum in cryopreservation of mesenchymal stromal cells?

    abstract：BACKGROUND AIMS:The therapeutic potential of human mesenchymal stromal cells (MSCs) has generated considerable interest in a wide variety of areas. MSC banking is feasible, but the optimal technique of cryopreservation remains to be determined. METHODS:To reduce dimethyl sulfoxide (DMSO) concentration in cryopreservat...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.06.012

    authors： Briquet A,Halleux A,Lechanteur C,Beguin Y

    更新日期：2013-11-01 00:00:00

  • Cytokine flow cytometry: multiparametric approach to immune function analysis.

    abstract：:More precise quantitation of cellular immune responses has become possible with the advent of single-cell assays of immune function, such as cytokine flow cytometry, enzyme-linked immunospot (ELISPOT), and MHC-peptide multimers. Cytokine flow cytometry is an attractive technique because it allows the detection of resp...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1080/14653240310000029

    authors： Ghanekar SA,Maecker HT

    更新日期：2003-01-01 00:00:00

  • Is the subarachnoid administration of mesenchymal stromal cells a useful strategy to treat chronic brain damage?

    abstract：BACKGROUND AIMS:Traumatic brain injury (TBI) is a leading cause of mortality and morbidity worldwide. Developing effective protocols for the administration of mesenchymal stromal cells (MSCs) is a promising therapeutic strategy to treat TBI. It is important to develop alternatives to direct parenchymal injection at the...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.07.007

    authors： Bonilla C,Zurita M,Aguayo C,Rodríguez A,Vaquero J

    更新日期：2014-11-01 00:00:00

  • Immunogenic potential of human bone marrow mesenchymal stromal cells is enhanced by hyperthermia.

    abstract：BACKGROUND AIMS:Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to suppress T-cell proliferation and used to alleviate the symptoms of graft-versus-host disease (GVHD). MSCs are a mixed cell population and at this time there are no tools to isolate the cells responsible for the T-cell suppressio...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2018.10.002

    authors： McClain-Caldwell I,Vitale-Cross L,Mayer B,Krepuska M,Boyajian M,Myneni V,Martin D,GENOMICS AND COMPUTATIONAL BIOLOGY CORE.,Marko K,Nemeth K,Mezey E

    更新日期：2018-12-01 00:00:00

  • BM-derived cells restore expression of peroxiredoxin V in the airways following acute naphthalene injury in mice.

    abstract：BACKGROUND:Naphthalene-induced respiratory tract toxicity in mice is characterized by specific and rapid loss of the Clara cell population, which is restored only after several days. The sources of restoration of this cell population remain unclear. We investigated whether BM-derived cells participated in the process o...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240500361012

    authors： Serikov VB,Popov BV,Kropotov AV,Tomilin NV

    更新日期：2005-01-01 00:00:00

  • Highly enriched cardiomyocytes from human embryonic stem cells.

    abstract：BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240802105307

    authors： Xu XQ,Zweigerdt R,Soo SY,Ngoh ZX,Tham SC,Wang ST,Graichen R,Davidson B,Colman A,Sun W

    更新日期：2008-01-01 00:00:00

  • Suicide genes as safety switches in T lymphocytes.

    abstract：:Broader application of adoptive transfer of tumor-specific T-lymphocytes is accompanied by the need for effective suicide genes to ensure the safety of this cell-based therapy. In vivo elimination of T-lymphocytes expressing the herpes simplex virus-derived thymidine kinase gene has demonstrated the feasibility of thi...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1080/14653240310001497

    authors： Straathof KC,Spencer DM,Sutton RE,Rooney CM

    更新日期：2003-01-01 00:00:00

  • Addition of varicella zoster virus-specific T cells to cytomegalovirus, Epstein-Barr virus and adenovirus tri-specific T cells as adoptive immunotherapy in patients undergoing allogeneic hematopoietic stem cell transplantation.

    abstract：BACKGROUND AIMS:Virus-specific T-cell immunotherapy is emerging as a promising management strategy for virus infections in patients after hematopoietic stem cell transplant (HSCT). Here we present outcomes of 10 adult patients who received multi-virus-specific T cells prophylactically after HSCT. METHODS:Donor-derived...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.07.005

    authors： Ma CK,Blyth E,Clancy L,Simms R,Burgess J,Brown R,Deo S,Micklethwaite KP,Gottlieb DJ

    更新日期：2015-10-01 00:00:00

  • Clinical analysis of the treatment of spinal cord injury with umbilical cord mesenchymal stem cells.

    abstract：BACKGROUND AIMS:The purpose of this study was to observe the clinical effect and safety of umbilical cord mesenchymal stem cells (UC-MSCs) in treating spinal cord injury (SCI) by intrathecal injection. METHODS:From January 2008 to October 2010, we treated 22 patients with SCI with UC-MSCs by intrathecal injection; dos...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2012.09.005

    authors： Liu J,Han D,Wang Z,Xue M,Zhu L,Yan H,Zheng X,Guo Z,Wang H

    更新日期：2013-02-01 00:00:00