当前位置： SCI文献检索 > CYTOTHERAPY期刊下所有文献 > Large-scale production and directed induction of functional dendritic cells ex vivo from serum-free expanded human hematopoietic stem cells.

Large-scale production and directed induction of functional dendritic cells ex vivo from serum-free expanded human hematopoietic stem cells.

Abstract：

BACKGROUND:Dendritic cells (DCs) that are derived from hematopoietic stem cells (HSCs) are the most potent antigen-presenting cells and play a pivotal role in initiating the immune response. Hence, large-scale production and direct induction of functional DCs ex vivo from HSCs are crucial to HSC research and clinical potential, such as vaccines for cancer and immune therapy. METHODS:In a previous study, we developed a serum-free HSC expansion system (SF-HSC medium) to expand large numbers of primitive HSCs ex vivo. Herein, a DC induction and expansion medium (DC medium) was proposed to further generate large numbers of functional DCs from serum-free expanded HSCs, which were developed and optimized by factorial design and the steepest ascent method. RESULTS:The DC medium is composed of effective basal medium (Iscove's modified Dulbecco's medium [IMDM]) and cytokines (2.9 ng/mL stem cell factor [SCF], 2.1 ng/mL Flt-3 ligand, 3.6 ng/mL interleukin [IL]-1β, 19.3 ng/mL granulocyte-macrophage colony-stimulating factor [GM-CSF] and 20.0 ng/mL tumor necrosis factor-α [TNF-α]). After 10-day culture in DC medium, the maximum fold expansion for accumulated CD1a+CD11c+ DCs was more than 4000-fold, and the induced DCs were characterized and confirmed by analysis of growth kinetics, surface antigen expression, endocytosis ability, mixed lymphocyte reaction, specific cytokine secretion and lipopolysaccharide stimulation. DISCUSSION:In conclusion, the combination of DC medium and SF-HSC medium can efficiently induce and expand a large amount of functional DCs from a small scale of HSCs and might be a promising source of DCs for vaccine and immune therapy in the near future.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

Hsu SC,Lu LC,Chan KY,Huang CH,Cheng SL,Chan YS,Yang YS,Lai YT,Yao CL

doi

10.1016/j.jcyt.2019.04.059

subject

Has Abstract

pub_date

2019-07-01 00:00:00

pages

755-768

issue

7

eissn

1465-3249

issn

1477-2566

pii

S1465-3249(19)30749-2

journal_volume

21

pub_type

杂志文章

相关文献

CYTOTHERAPY文献大全
  • Combined cellular immunotherapy and chemotherapy improves clinical outcome in patients with gastric carcinoma.

    abstract：BACKGROUND AIMS:Despite the availability of multiple treatment strategies, patients with gastric carcinoma (GC) have a dismal prognosis. The aim of this study was to evaluate the efficacy and safety of cellular immunotherapy (CIT) with the use of autologous natural killer cells, γδT cells and cytokine-induced killer ce...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.03.605

    authors： Cui J,Li L,Wang C,Jin H,Yao C,Wang Y,Li D,Tian H,Niu C,Wang G,Han W,Xu J,Chen J,Li W

    更新日期：2015-07-01 00:00:00

  • Transplantation with low-density autologous PBSC prepared with BDS60 for women with Stage II, III and IV breast cancer.

    abstract：BACKGROUND:DS60 is a novel buoyant density solution, whose density has been adjusted to enrich PBSC from subjects who have been mobilized with cytokines alone, or cytokines plus chemotherapy. This report describes the use of BDS60 to enrich autologous PBSC that were used for hematological reconstitution after myeloabla...

    journal_title：Cytotherapy

    pub_type： 临床试验,杂志文章

    doi：10.1080/146532400539134

    authors： Laport GG,Valone FH,Zimmerman TM,Grinblatt DL,Van Vlasselaer P,Still BJ,Williams SF

    更新日期：2000-01-01 00:00:00

  • Intravenous infusion of adipose-derived stem/stromal cells improves functional recovery of rats with spinal cord injury.

    abstract：BACKGROUND AIMS:Adipose tissue has therapeutic potential for spinal cord injury (SCI) because it contains multipotent cells known as adipose-derived stem/stromal cells (ASCs). In this study, we attempted intravenous ASC transplantation in rats with SCI to examine the effect on functional recovery. METHODS:ASCs (2.5 × ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2017.04.002

    authors： Ohta Y,Hamaguchi A,Ootaki M,Watanabe M,Takeba Y,Iiri T,Matsumoto N,Takenaga M

    更新日期：2017-07-01 00:00:00

  • Closed-system manufacturing of CD19 and dual-targeted CD20/19 chimeric antigen receptor T cells using the CliniMACS Prodigy device at an academic medical center.

    abstract：BACKGROUND AIMS:Multiple steps are required to produce chimeric antigen receptor (CAR)-T cells, involving subset enrichment or depletion, activation, gene transduction and expansion. Open processing steps that increase risk of contamination and production failure are required. This complex process requires skilled pers...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2017.09.005

    authors： Zhu F,Shah N,Xu H,Schneider D,Orentas R,Dropulic B,Hari P,Keever-Taylor CA

    更新日期：2018-03-01 00:00:00

  • Highly enriched cardiomyocytes from human embryonic stem cells.

    abstract：BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240802105307

    authors： Xu XQ,Zweigerdt R,Soo SY,Ngoh ZX,Tham SC,Wang ST,Graichen R,Davidson B,Colman A,Sun W

    更新日期：2008-01-01 00:00:00

  • HLA tetramers and anti-CMV immune responses: from epitope to immunotherapy.

    abstract：BACKGROUND:Identification of HLA class I-restricted CMV epitopes, and the subsequent synthesis of HLA class I-peptide tetrameric complexes, have provided investigators with an important tool for visualising and quantifying the precise in vivo CTL response to CMV reactivation following stem cell transplantation. In conj...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/146532402317251518

    authors： Chen FE,Aubert G,Travers P,Dodi IA,Madrigal JA

    更新日期：2002-01-01 00:00:00

  • Summary of the National Institute of Standards and Technology and US Food And Drug Administration cell counting workshop: Sharing practices in cell counting measurements.

    abstract：:The emergence of cell-based therapeutics has increased the need for high-quality, robust and validated measurements for cell characterization. Cell count, being one of the most fundamental measures for cell-based therapeutics, now requires increased levels of measurement confidence. The National Institute of Standards...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2018.03.031

    authors： Lin-Gibson S,Sarkar S,Elliott JT

    更新日期：2018-06-01 00:00:00

  • A strategy for single nucleotide polymorphism analysis of chimerism for somatic cell therapy.

    abstract：BACKGROUND AIMS:Chimerism is an important outcome measure in hematopoietic cell transplantation as well as somatic cell therapy. Commonly used methods to estimate chimerism are restricted by either gender or inefficient sensitivity. In principle, real-time polymerase chain reaction (PCR)-based assays can be used to ass...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653241003774029

    authors： Chen X,Hofmann TJ,Otsuru S,Jethva R,Lind C,Monos D,Horwitz EM

    更新日期：2010-12-01 00:00:00

  • Exosome-transmitted LINC00461 promotes multiple myeloma cell proliferation and suppresses apoptosis by modulating microRNA/BCL-2 expression.

    abstract：BACKGROUND:Multiple myeloma (MM) is a hematologic cancer caused by the abnormal expansion of plasma cells, but the exact mechanism underlying MM development is not completely known. Recently, multiple long noncoding RNAs (lncRNAs) were implicated in the regulation of MM development. METHODS:Samples from patients with ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2018.10.006

    authors： Deng M,Yuan H,Liu S,Hu Z,Xiao H

    更新日期：2019-01-01 00:00:00

  • Phenotypic shift of human amniotic epithelial cells in culture is associated with reduced osteogenic differentiation in vitro.

    abstract：BACKGROUND:Amniotic membrane is a highly promising cell source for tissue engineering. Being part of the placenta, this tissue is abundantly available. It can be processed easily to yield large amounts of epithelial and mesenchymal cells that have shown broad differentiation potential. For tissue-engineering purposes, ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240802345804

    authors： Stadler G,Hennerbichler S,Lindenmair A,Peterbauer A,Hofer K,van Griensven M,Gabriel C,Redl H,Wolbank S

    更新日期：2008-01-01 00:00:00

  • Mesenchymoangioblast-derived mesenchymal stromal cells inhibit cell damage, tissue damage and improve peripheral blood flow following hindlimb ischemic injury in mice.

    abstract：BACKGROUND AIMS:Existing treatments have limited success in modifying the course of peripheral artery disease, which may eventually lead to limb-threatening ulcers and amputation. Cellular therapies have the potential to provide a new treatment option for this condition, but isolation of cells by conventional means has...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.10.013

    authors： Koch JM,D'Souza SS,Schwahn DJ,Dixon I,Hacker TA

    更新日期：2016-02-01 00:00:00

  • Genomic and functional comparison of mesenchymal stromal cells prepared using two isolation methods.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been applied to patients in cell therapy for various diseases. Recently, we introduced a novel MSC separation filter device which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system compared with the conventional open density gradient ce...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.10.013

    authors： Otsuru S,Hofmann TJ,Raman P,Olson TS,Guess AJ,Dominici M,Horwitz EM

    更新日期：2015-03-01 00:00:00

  • A decade of marketing approval of gene and cell-based therapies in the United States, European Union and Japan: An evaluation of regulatory decision-making.

    abstract：:There is a widely held expectation of clinical advance with the development of gene and cell-based therapies (GCTs). Yet, establishing benefits and risks is highly uncertain. We examine differences in decision-making for GCT approval between jurisdictions by comparing regulatory assessment procedures in the United Sta...

    journal_title：Cytotherapy

    pub_type： 历史文章,杂志文章

    doi：10.1016/j.jcyt.2018.03.038

    authors： Coppens DGM,de Wilde S,Guchelaar HJ,De Bruin ML,Leufkens HGM,Meij P,Hoekman J

    更新日期：2018-06-01 00:00:00

  • Is the subarachnoid administration of mesenchymal stromal cells a useful strategy to treat chronic brain damage?

    abstract：BACKGROUND AIMS:Traumatic brain injury (TBI) is a leading cause of mortality and morbidity worldwide. Developing effective protocols for the administration of mesenchymal stromal cells (MSCs) is a promising therapeutic strategy to treat TBI. It is important to develop alternatives to direct parenchymal injection at the...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.07.007

    authors： Bonilla C,Zurita M,Aguayo C,Rodríguez A,Vaquero J

    更新日期：2014-11-01 00:00:00

  • Current state of U.S. Food and Drug Administration regulation for cellular and gene therapy products: potential cures on the horizon.

    abstract：:Cellular & Gene Therapies (CGTs) are complex products, which have been key foci of the International Society for Cell & Gene Therapy (ISCT). For this ISCT North American Legal & Regulatory Affairs Committee review publication, CGTs include but are not limited to somatic cell-based therapies, pluripotent cell-derived c...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2019.04.002

    authors： Mendicino M,Fan Y,Griffin D,Gunter KC,Nichols K

    更新日期：2019-07-01 00:00:00

  • Adipose-derived mesenchymal stromal cells from genetically modified pigs: immunogenicity and immune modulatory properties.

    abstract：BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.651529

    authors： Kumar G,Hara H,Long C,Shaikh H,Ayares D,Cooper DK,Ezzelarab M

    更新日期：2012-04-01 00:00:00

  • Induction of potent protection against acute and latent herpes simplex virus infection in mice vaccinated with dendritic cells.

    abstract：BACKGROUND AIMS:Dendritic cells (DCs) are the most potent antigen presenting cells of the immune system and have been under intense study with regard to their use in immunotherapy against cancer and infectious disease agents. In the present study, DCs were employed to assess their value in protection against live virus...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2012.11.012

    authors： Ghasemi M,Erturk M,Buruk K,Sonmez M

    更新日期：2013-03-01 00:00:00

  • Neuropeptides to replace serum in cryopreservation of mesenchymal stromal cells?

    abstract：BACKGROUND AIMS:The therapeutic potential of human mesenchymal stromal cells (MSCs) has generated considerable interest in a wide variety of areas. MSC banking is feasible, but the optimal technique of cryopreservation remains to be determined. METHODS:To reduce dimethyl sulfoxide (DMSO) concentration in cryopreservat...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.06.012

    authors： Briquet A,Halleux A,Lechanteur C,Beguin Y

    更新日期：2013-11-01 00:00:00

  • Toll-like receptor 3 pre-conditioning increases the therapeutic efficacy of umbilical cord mesenchymal stromal cells in a dextran sulfate sodium-induced colitis model.

    abstract：BACKGROUND AIMS:Immunomodulatory properties of human umbilical cord-derived mesenchymal stromal cells (UCMSCs) can be differentially modulated by toll-like receptors (TLR) agonists. Here, the therapeutic efficacy of short TLR3 and TLR4 pre-conditioning of UCMSCs was evaluated in a dextran sulfate sodium (DSS)-induced c...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.02.002

    authors： Fuenzalida P,Kurte M,Fernández-O'ryan C,Ibañez C,Gauthier-Abeliuk M,Vega-Letter AM,Gonzalez P,Irarrázabal C,Quezada N,Figueroa F,Carrión F

    更新日期：2016-05-01 00:00:00

  • Long-term outcome of a phase II study of BM transplants, partially depleted ex-vivo of CD5-positive and CD8-positive T-lymphocytes in unrelated and related donor 1 antigen mismatched recipients.

    abstract：BACKGROUND:Mismatched family donor and unrelated donor BM transplants are associated with a high risk of acute GvHD. White T-cell depletion is the best method to reduce risk of acute GvHD, there was a reluctance to use T-cell depletion in the mismatched setting because of increased risk of rejection and relapse. Partia...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/0032472031000141284

    authors： Gajewski JL,Nimer S,Saliba RM,Thomas M,Przepiorka D,Giralt S,von Besien K,Mehra R,Andersson B,Chan KW,Ippoliti C,Warkinten D,Feigs S,Territo M,Schiller G,Lebkowski J,Moseley AM,Lloyd K,von Hoeff M,Okarma T,Champli

    更新日期：1999-01-01 00:00:00

  • Human mesenchymal stromal cells transiently increase cytokine production by activated T cells before suppressing T-cell proliferation: effect of interferon-γ and tumor necrosis factor-α stimulation.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) suppress T-cell proliferation, especially after activation with inflammatory cytokines. We compared the dynamic action of unprimed and interferon (IFN)-γ plus tumor necrosis factor (TNF)-α-pretreated human bone marrow-derived MSCs on resting or activated T cells. METHOD...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.11.008

    authors： Cuerquis J,Romieu-Mourez R,François M,Routy JP,Young YK,Zhao J,Eliopoulos N

    更新日期：2014-02-01 00:00:00

  • Ex vivo immunological evaluation of stable mixed chimeric patients after matched related donor allogeneic transplantation in sickle cell disease.

    abstract：BACKGROUND AIMS:Allogeneic hematopoietic stem cell transplantation is curative for sickle cell disease, and the use of matched related donors, non-myeloablative conditioning and sirolimus immunosuppression results in stable mixed chimerism without graft-versus-host disease (GVHD). However, the time to terminate sirolim...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2019.10.003

    authors： Raines LN,Hsieh MM,Nassehi T,Drysdale CM,Tisdale JF,Uchida N

    更新日期：2019-12-01 00:00:00

  • Autologous mesenchymal stromal cell transplantation for spinal cord injury: A Phase I pilot study.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cell (MSC) transplantation has immerged as promising therapeutic approach to treat spinal cord injury (SCI). In this pilot study, we investigated the safety of intrathecal injection of autologous bone marrow-derived MSCs in nine patients with SCI. METHODS:Patients with complete SCI ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.01.004

    authors： Satti HS,Waheed A,Ahmed P,Ahmed K,Akram Z,Aziz T,Satti TM,Shahbaz N,Khan MA,Malik SA

    更新日期：2016-04-01 00:00:00

  • Neural stem cells transplanted to the subretinal space of rd1 mice delay retinal degeneration by suppressing microglia activation.

    abstract：BACKGROUND AIMS:Retinal degeneration (RD) is an inherited eye disease characterized by irreversible photoreceptor loss. Conventionally, the activation of the resident microglia is secondary to the disease. Stem cell-based therapy has recently made rapid progress in treating RD. Although it has been demonstrated that th...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.03.001

    authors： Li Z,Zeng Y,Chen X,Li Q,Wu W,Xue L,Xu H,Yin ZQ

    更新日期：2016-06-01 00:00:00

  • Improved expansion of T cells in culture when isolated with an equipment-free, high-throughput, flow-through microfluidic module versus traditional density gradient centrifugation.

    abstract：BACKGROUND:The isolation of lymphocytes - and removal of platelets (PLTs) and red blood cells (RBCs) - from an initial blood sample prior to culture is a key enabling step for effective manufacture of cellular therapies. Unfortunately, currently available methods suffer from various drawbacks, including low cell recove...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2018.12.004

    authors： Strachan BC,Xia H,Vörös E,Gifford SC,Shevkoplyas SS

    更新日期：2019-02-01 00:00:00

  • International Society for Cellular Therapy perspective on immune functional assays for mesenchymal stromal cells as potency release criterion for advanced phase clinical trials.

    abstract：:Mesenchymal stromal cells (MSCs) as a pharmaceutical for ailments characterized by pathogenic autoimmune, alloimmune and inflammatory processes now cover the spectrum of early- to late-phase clinical trials in both industry and academic sponsored studies. There is a broad consensus that despite different tissue sourci...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.11.008

    authors： Galipeau J,Krampera M,Barrett J,Dazzi F,Deans RJ,DeBruijn J,Dominici M,Fibbe WE,Gee AP,Gimble JM,Hematti P,Koh MB,LeBlanc K,Martin I,McNiece IK,Mendicino M,Oh S,Ortiz L,Phinney DG,Planat V,Shi Y,Stroncek DF,Vi

    更新日期：2016-02-01 00:00:00

  • Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System.

    abstract：BACKGROUND AIMS:The Quantum® Cell Expansion System (Quantum; Terumo BCT, Inc, Lakewood, CO, USA) is a novel hollow fiber-based device that automates and closes the cell culture process, reducing labor intensive tasks such as manual cell culture feeding and harvesting. The manual cell selection and expansion processes f...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.05.024

    authors： Jones M,Varella-Garcia M,Skokan M,Bryce S,Schowinsky J,Peters R,Vang B,Brecheisen M,Startz T,Frank N,Nankervis B

    更新日期：2013-11-01 00:00:00

  • Hepatocyte growth factor enhances the inflammation-alleviating effect of umbilical cord-derived mesenchymal stromal cells in a bronchiolitis obliterans model.

    abstract：BACKGROUND AIMS:Specific and effective therapy for prevention or reversal of bronchiolitis obliterans (BO) is lacking. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF) gene modified mesenchymal stromal cells (MSCs) on BO. METHODS:A mouse model of experimental BO was established by s...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2015.12.006

    authors： Cao XP,Han DM,Zhao L,Guo ZK,Xiao FJ,Zhang YK,Zhang XY,Wang LS,Wang HX,Wang H

    更新日期：2016-03-01 00:00:00

  • Development of a surrogate angiogenic potency assay for clinical-grade stem cell production.

    abstract：BACKGROUND AIMS:Clinical results from acute myocardial infarction (AMI) patients treated with MultiStem®, a large-scale expanded adherent multipotent progenitor cell population (MAPC), have demonstrated a strong safety and benefit profile for these cells. The mechanism of benefit with MAPC treatment is a result, in par...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2012.688945

    authors： Lehman N,Cutrone R,Raber A,Perry R,Van't Hof W,Deans R,Ting AE,Woda J

    更新日期：2012-09-01 00:00:00

  • Yield and proliferation rate of adipose-derived stromal cells as a function of age, body mass index and harvest site-increasing the yield by use of adherent and supernatant fractions?

    abstract：BACKGROUND AIMS:Adipose-derived stem cells are easily accessed and have a relatively high density compared with other mesenchymal stromal cells. Isolation protocols of adipose-derived stem cells (ASC) rely on the cell's ability to adhere to tissue culture plastic overnight. It was evaluated whether the floating ASC fra...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.04.009

    authors： Buschmann J,Gao S,Härter L,Hemmi S,Welti M,Werner CM,Calcagni M,Cinelli P,Wanner GA

    更新日期：2013-09-01 00:00:00