Closed-system manufacturing of CD19 and dual-targeted CD20/19 chimeric antigen receptor T cells using the CliniMACS Prodigy device at an academic medical center.

abstract：BACKGROUND AIMS:Adipose tissue has therapeutic potential for spinal cord injury (SCI) because it contains multipotent cells known as adipose-derived stem/stromal cells (ASCs). In this study, we attempted intravenous ASC transplantation in rats with SCI to examine the effect on functional recovery. METHODS:ASCs (2.5 × ...

journal_title：Cytotherapy

authors： Ohta Y,Hamaguchi A,Ootaki M,Watanabe M,Takeba Y,Iiri T,Matsumoto N,Takenaga M

更新日期：2017-07-01 00:00:00

abstract：BACKGROUND:Bone marrow (BM)-derived mesenchymal stromal cells (MSC) are promising candidate cells for the development of neuroregenerative therapies. We have previously introduced the pro-neural conversion of human MSC to neural stem cell-like cells (m-NSC) by culturing them in suspension culture under serum-free condi...

journal_title：Cytotherapy

authors： Habisch HJ,Fiedler J,Ludolph AC,Storch A,Brenner RE

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Container integrity is critical for maintaining sterility of cryopreserved cellular therapy products. We investigated a series of catastrophic bag failures, first noticed in early 2001. METHODS:Process records were reviewed for all PBPC and lymphocyte products cryopreserved in bags from January 2000 through...

journal_title：Cytotherapy

authors： Khuu HM,Cowley H,David-Ocampo V,Carter CS,Kasten-Sportes C,Wayne AS,Solomon SR,Bishop MR,Childs RM,Read EJ

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND AIMS:Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSCs) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the...

journal_title：Cytotherapy

authors： Zimmerlin L,Rubin JP,Pfeifer ME,Moore LR,Donnenberg VS,Donnenberg AD

更新日期：2013-01-01 00:00:00

abstract：BACKGROUND AIMS:Virus-specific T-cell immunotherapy is emerging as a promising management strategy for virus infections in patients after hematopoietic stem cell transplant (HSCT). Here we present outcomes of 10 adult patients who received multi-virus-specific T cells prophylactically after HSCT. METHODS:Donor-derived...

journal_title：Cytotherapy

authors： Ma CK,Blyth E,Clancy L,Simms R,Burgess J,Brown R,Deo S,Micklethwaite KP,Gottlieb DJ

更新日期：2015-10-01 00:00:00

abstract：:For more than a decade, Ab conjugated to a radionuclide emitting particulate radiation has been used in the management of leukemia in an effort to deliver targeted doses of radiation to BM, spleen and other sites of disease, while sparing normal organs. This radioimmunotherapy (RIT) approach has been employed to achie...

journal_title：Cytotherapy

authors： Pagel JM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow (BM) mesenchymal stromal cells (MSC) represent a novel therapy for severe heart failure with extensive myocardial scarring, especially when performed concurrently with conventional revascularization. However, stem cells are difficult to transport in culture media without risk of contaminatio...

journal_title：Cytotherapy

authors： Chin SP,Poey AC,Wong CY,Chang SK,Teh W,Mohr TJ,Cheong SK

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Tumor cell lines have enormous value for the study of different aspects of cancer biology and have also recently gained great importance in autologous cell-based anti-tumor therapies. However, the use of these cells is still limited because in vitro growth is hampered by suboptimal culture conditions and cur...

journal_title：Cytotherapy

authors： Stadler G,Wieser M,Steindl F,Grillari J,Katinger H,Pfragner R,Voglauer R

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND AIMS:The microenvironment of the chronically injured spinal cord does not allow for axonal regeneration due to glial scarring. To ameliorate this, several therapeutic strategies have been used. We investigated whether combined transplantation of chondroitinase ABC (chABC) and mesenchymal stromal cells (MSCs)...

journal_title：Cytotherapy

authors： Lee SH,Kim Y,Rhew D,Kim A,Jo KR,Yoon Y,Choi KU,Jung T,Kim WH,Kweon OK

更新日期：2016-10-28 00:00:00

abstract：BACKGROUND AIMS:Imatinib (IM), a tyrosine kinase inhibitor targeting the BCR-ABL oncoprotein, remains a major therapeutic strategy for patients with chronic myelogenous leukemia (CML). However, IM resistance is still a challenge in the treatment of CML. Recently, it was reported that exosomes (Exo) were involved in dru...

journal_title：Cytotherapy

authors： Liu Y,Song B,Wei Y,Chen F,Chi Y,Fan H,Liu N,Li Z,Han Z,Ma F

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND AIMS:Umbilical cord blood (UCB) provides an alternative source for hematopoietic stem/progenitor cells (HSPCs) in the treatment of hematological malignancies. However, clinical usage is limited due to the low quantity of HSPCs in each unit of cord blood and defects in bone marrow homing. Hyperbaric oxygen (H...

journal_title：Cytotherapy

authors： Cheung KY,Berry A,Li D,Aljitawi OS

更新日期：2018-01-01 00:00:00

abstract：:Chimeric antigen receptor (CAR) T-cell therapy is an investigational immunocellular therapy that reprograms a patient's cytotoxic T cells to engage and eliminate malignant cells. CAR T-cell therapies targeting the CD19 antigen have demonstrated high efficacy in clinical trials for patients with B-cell malignancies and...

journal_title：Cytotherapy

authors： McGuirk J,Waller EK,Qayed M,Abhyankar S,Ericson S,Holman P,Keir C,Myers GD

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND AIMS:Although bone marrow c-kit(+) progenitor cells support myocardial regeneration, the cardiomyocyte differentiation potential of umbilical cord blood (UCB) c-kit(+) cells is unknown. METHODS:UCB mononuclear cells (MNCs) and c-kit(+) cells purified by use of immunomagnetic beads were used. Cardiomyocyte d...

journal_title：Cytotherapy

authors： Iachininoto MG,Capodimonti S,Podda MV,Valentini CG,Bianchi M,Leone AM,Teofili L,Leone G

更新日期：2015-11-01 00:00:00

abstract：:We report on a roundtable event hosted in Singapore that sought to identify some of the ethical and regulatory challenges in translating autologous cell-based interventions, particularly those claiming to involve stem cells, into safe and effective therapies and to propose some solutions to encourage responsible innov...

journal_title：Cytotherapy

authors： Lysaght T,Munsie M,Castricum A,Hui JHP,Okada K,Sato Y,Sawa Y,Stewart C,Tan LK,Tan LHY,Sugii S

更新日期：2018-09-01 00:00:00

abstract：BACKGROUND:PBSC transplant provides 10 times more T cells than BMT However, the incidence and severity of acute GvHD is similar among recipients of both types of transplants. Studies in mouse models suggest that the similar clinical outcome in BMT and PBSCT is due to differences in the lymphokine profiles. METHODS:PBM...

journal_title：Cytotherapy

authors： Vasconcelos ZF,Santos BM,Costa ES,Lima M,Tabak DG,Bouzas LF,Azevedo WM,Barcinski MA,Bonomo A

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND AIMS:Circulating monocytes have been exploited as an important progenitor cell resource for hepatocytes in vitro and are instrumental in the removal of fibrosis. We investigated the significance of monocytes in peripheral blood stem cells (PBSC) for the treatment of liver cirrhosis. METHODS:Rat CD14+ monocy...

journal_title：Cytotherapy

authors： Wang J,Zhou X,Cui L,Yan L,Liang J,Cheng X,Qiao L,Shi Y,Han Z,Cao Y,Han Y,Fan D

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) are gaining in popularity as an experimental therapy for a number of conditions that often require expansion ex vivo prior to use. Data comparing clinical-grade MSC from various ages of donors are scant. We hypothesized that MSC from older donors may display differences i...

journal_title：Cytotherapy

authors： Lund TC,Kobs A,Blazar BR,Tolar J

更新日期：2010-12-01 00:00:00

abstract：BACKGROUND AIMS:Defocused low-energy shock wave (DLSW) therapy has shown effectiveness in regenerative medicine. The mechanism of action was mainly focused on the pathophysiological improvement at the wound tissues. In this study, the activation of stem cells treated by DLSW was first examined as an important pathway d...

journal_title：Cytotherapy

authors： Zhao Y,Wang J,Wang M,Sun P,Chen J,Jin X,Zhang H

更新日期：2013-12-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow-derived mesenchymal stromal cells (MSCs) have been reported to suppress T-cell proliferation and used to alleviate the symptoms of graft-versus-host disease (GVHD). MSCs are a mixed cell population and at this time there are no tools to isolate the cells responsible for the T-cell suppressio...

journal_title：Cytotherapy

authors： McClain-Caldwell I,Vitale-Cross L,Mayer B,Krepuska M,Boyajian M,Myneni V,Martin D,GENOMICS AND COMPUTATIONAL BIOLOGY CORE.,Marko K,Nemeth K,Mezey E

更新日期：2018-12-01 00:00:00

abstract：:Initially used as an alternative hematopoietic stem cell source for patients without a human leukocyte antigen-matched bone marrow or peripheral blood stem cell donor, unrelated cord blood (UCB) is now the preferred donor source when hematopoietic stem cell transplantation (HSCT) is used to treat patients with lysosom...

journal_title：Cytotherapy

authors： Aldenhoven M,Kurtzberg J

更新日期：2015-06-01 00:00:00

abstract：:Not all patients with Hodgkin's disease (HD) respond to standard chemotherapy and/or radiation, and hematopoietic stem-cell transplantation has been gaining increasing acceptance in the management of HD. Phase II and, to a lesser extent, phase III studies of high-dose chemotherapy and autologous stem cell transplantat...

journal_title：Cytotherapy

authors： Anderlini P

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress respons...

journal_title：Cytotherapy

authors： Mendoza-Gamboa E,Franco-Molina MA,Zapata-Benavides P,Castillo-Tello P,Vera-García ME,Tamez-Guerra RS,Rodríguez-Padilla C

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Existing treatments have limited success in modifying the course of peripheral artery disease, which may eventually lead to limb-threatening ulcers and amputation. Cellular therapies have the potential to provide a new treatment option for this condition, but isolation of cells by conventional means has...

journal_title：Cytotherapy

authors： Koch JM,D'Souza SS,Schwahn DJ,Dixon I,Hacker TA

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:Multiple myeloma (MM) is a hematologic cancer caused by the abnormal expansion of plasma cells, but the exact mechanism underlying MM development is not completely known. Recently, multiple long noncoding RNAs (lncRNAs) were implicated in the regulation of MM development. METHODS:Samples from patients with ...

journal_title：Cytotherapy

authors： Deng M,Yuan H,Liu S,Hu Z,Xiao H

更新日期：2019-01-01 00:00:00

abstract：BACKGROUND:Cell therapy has been proposed as a salvage limb procedure in critical limb ischemia (CLI). In spite of the fact that clinical trials found some efficacy, the mechanism of action remains elusive. The objective of this study was to characterize two autologous cell therapy products (CTPs) obtained from patient...

journal_title：Cytotherapy

authors： Tournois C,Pignon B,Sevestre MA,Al-Rifai R,Creuza V,Poitevin G,François C,Nguyen P

更新日期：2017-02-01 00:00:00

abstract：BACKGROUND:BM mesenchymal stem cells (MSC) have the capacity for renewal and the potential to differentiate into multiple tissues. In this study, we compared different enrichment methods to obtain MSC from BM. METHODS:Three different methods were compared with a view to obtaining MSC more rapidly from BM: negative sel...

journal_title：Cytotherapy

authors： Tondreau T,Lagneaux L,Dejeneffe M,Delforge A,Massy M,Mortier C,Bron D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND AIMS:T cells can be redirected to reject cancer by retroviral transduction with a chimeric antigen receptor (CAR) or by administration of a bispecific T cell engager (BiTE). We demonstrate that transfection of T cells with messenger (m) RNA coding for CAR is an alternative strategy. METHODS:We describe the ...

journal_title：Cytotherapy

authors： Almåsbak H,Rian E,Hoel HJ,Pulè M,Wälchli S,Kvalheim G,Gaudernack G,Rasmussen AM

更新日期：2011-05-01 00:00:00

abstract：:Kidney disease has reached epidemic proportions and is associated with high mortality and morbidity rates. Stem cell-based therapy may effectively treat kidney damage by cell transplantation. The breakthrough discovery using a combination of four transcription factors to reprogram genetically somatic cells into induce...

journal_title：Cytotherapy

authors： Wang WW,Wang W,Jiang Y,Han GF,Lu S,Li G,Zhang J

更新日期：2013-05-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been studied intensively in regenerative medicine. However, their therapeutic potential against tumor formation and cancer metastasis is still unclear. The effects of transplantation of MSCs in early-stage of carcinogenesis, should be evaluated. METHODS:MSC isolated...

journal_title：Cytotherapy

authors： Sun B,Roh KH,Park JR,Lee SR,Park SB,Jung JW,Kang SK,Lee YS,Kang KS

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Enumeration of CD34(+) cells in leukocyte-rich cell suspensions is important for clinical decision-making in stem cell transplantation. Single-platform flow cytometry assays offer the significant advantages of speed and reproducibility, and have therefore become the gold standard in stem cell enumeratio...

journal_title：Cytotherapy

authors： Dauber K,Becker D,Odendahl M,Seifried E,Bonig H,Tonn T

更新日期：2011-04-01 00:00:00