Abstract:
BACKGROUND:We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress response, apoptosis and survival. It remains unknown whether AP-1 and other transcription factors are mechanisms by which bDLE induces cell death. METHODS:To determine whether bDLE modulates the AP-1 DNA binding and gene expression, MCF-7 breast cancer cells were treated with bDLE (0, 1, 5, 10 U) for 72 h and evaluated by electrophoretic mobility shift assay, reverse transcriptase-polymerase chain reaction and Western blot assays. RESULTS:bDLE induced inhibition of cell growth, suppressed the AP-1 DNA-binding activity, decreased c-Jun protein expression and modulated NFATx, NFATc, NFkappaB, c-Jun and c-Fos transcription factor gene expression in MCF-7 breast cancer cells. DISCUSSION:The present data indicate that bDLE can block the AP-1 DNA-binding activity and expression of several transcriptions factors in breast cancer cells, which will have great potential in improving cancer therapy.
journal_name
Cytotherapyjournal_title
Cytotherapyauthors
Mendoza-Gamboa E,Franco-Molina MA,Zapata-Benavides P,Castillo-Tello P,Vera-García ME,Tamez-Guerra RS,Rodríguez-Padilla Cdoi
10.1080/14653240801891659subject
Has Abstractpub_date
2008-01-01 00:00:00pages
212-9issue
2eissn
1465-3249issn
1477-2566pii
790554404journal_volume
10pub_type
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