当前位置： SCI文献检索 > CYTOTHERAPY期刊下所有文献 > Exosomes from mesenchymal stromal cells enhance imatinib-induced apoptosis in human leukemia cells via activation of caspase signaling pathway.

Exosomes from mesenchymal stromal cells enhance imatinib-induced apoptosis in human leukemia cells via activation of caspase signaling pathway.

Abstract：

BACKGROUND AIMS:Imatinib (IM), a tyrosine kinase inhibitor targeting the BCR-ABL oncoprotein, remains a major therapeutic strategy for patients with chronic myelogenous leukemia (CML). However, IM resistance is still a challenge in the treatment of CML. Recently, it was reported that exosomes (Exo) were involved in drug resistance. Therefore, the present study investigated whether Exo secreted by human umbilical cord mesenchymal stromal cells (hUC-MSC-Exo) affected the sensitivity of K562 cells to IM. METHODS:hUC-MSC-Exo were isolated and identified. K562 cells were then treated or not with IM (1 µmol/L) in combination with hUC-MSC-Exo (50 µg/mL). Cell viability and apoptosis were determined by cell counting kit 8 (CCK-8) and annexin V/propidium iodide (PI) double staining, respectively. Apoptotic proteins, caspase and their cleaved forms were detected by Western blot. RESULTS:It was shown that hUC-MSC-Exo alone had no effect on cell viability and apoptosis of K562 cells. However, hUC-MSC-Exo promoted IM-induced cell viability inhibition and apoptosis. Moreover, hUC-MSC-Exo enhanced the increased Bax expression and the decreased Bcl-2 expression that were induced by IM. Compared with IM alone, caspase-9 and caspase-3 were further activated by combination of hUC-MSC-Exo with IM. Finally, the effects of hUC-MSC-Exo on K562 cells could be reversed by pretreatment of K562 cells with caspase inhibitor Z-VAD-FMK (30 µmol/L) DISCUSSION: These results indicate that hUC-MSC-Exo enhanced the sensitivity of K562 cells to IM via activation of caspase signaling pathway. Therefore, combining IM with hUC-MSC-Exo could be a promising approach to improve the efficacy of CML treatment.

journal_name

Cytotherapy

journal_title

Cytotherapy

authors

Liu Y,Song B,Wei Y,Chen F,Chi Y,Fan H,Liu N,Li Z,Han Z,Ma F

doi

10.1016/j.jcyt.2017.11.006

subject

Has Abstract

pub_date

2018-02-01 00:00:00

pages

181-188

issue

2

eissn

1465-3249

issn

1477-2566

pii

S1465-3249(17)30733-8

journal_volume

20

pub_type

杂志文章

相关文献

CYTOTHERAPY文献大全
  • Protein profiling of anastomosed facial nerve treated with mesenchymal stromal cells.

    abstract：BACKGROUND AIMS:The types of proteins released from mesenchymal stromal cells (MSC) are still unclear. Our aim was to compare apoptosis scores and the expression of myelin-associated glycoprotein (MAG), myelin basic protein (MBP), neural cell adhesion molecule (NCAM)-1,matrix metalloproteinase (MMP)-1A, tissue inhibito...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.651530

    authors： Satar B,Hidir Y,Serdar MA,Kucuktag Z,Ural AU,Avcu F,Safali M,Oguztuzun S

    更新日期：2012-05-01 00:00:00

  • Manufacture and quality control of CAMPATH-1 antibodies for clinical trials.

    abstract：BACKGROUND:CAMPATH-1 Abs have been used for T-cell depletion in stem-cell transplantation since the early 1980s. During that time there has been substantial progress in manufacturing techniques and quality control procedures. This article summarizes the methods used to produce the Abs for clinical use and describes res...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/146532401753174061

    authors： Phillips J,Drumm A,Harrison P,Bird P,Bhamra K,Berrie E,Hale G

    更新日期：2001-01-01 00:00:00

  • Screening of genes responsible for differentiation of mouse mesenchymal stromal cells by DNA micro-array analysis of C3H10T1/2 and C3H10T1/2-derived cell lines.

    abstract：BACKGROUND:The molecular mechanisms underlying the biologic effects or differentiation of mesenchymal stromal cells (MSC) have not been clarified. Screening for genes differentially expressed at different stages is an important step in determining these molecular mechanisms. METHODS:In this study, we analyzed the gene...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240601016374

    authors： Oh I,Ozaki M,Miyazato A,Sato K,Meguro A,Muroi K,Nagai T,Mano H,Ozawa K

    更新日期：2007-01-01 00:00:00

  • A robust, good manufacturing practice-compliant, clinical-scale procedure to generate regulatory T cells from patients with amyotrophic lateral sclerosis for adoptive cell therapy.

    abstract：:Regulatory T cells (Tregs) play a fundamental role in the maintenance of self-tolerance and immune homeostasis. Defects in Treg function and/or frequencies have been reported in multiple disease models. Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting upper and lower motor neur...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.06.012

    authors： Alsuliman A,Appel SH,Beers DR,Basar R,Shaim H,Kaur I,Zulovich J,Yvon E,Muftuoglu M,Imahashi N,Kondo K,Liu E,Shpall EJ,Rezvani K

    更新日期：2016-10-01 00:00:00

  • Adipose-derived mesenchymal stromal cells from genetically modified pigs: immunogenicity and immune modulatory properties.

    abstract：BACKGROUND AIMS:The immunomodulatory and anti-inflammatory effects of mesenchymal stromal cells (MSC) could prove to be a potential therapeutic approach for prolongation of survival of cell xenotransplantation. Adipose (Ad) MSC from genetically modified pigs could be an abundant source of pig donor-specific MSC. METHO...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.651529

    authors： Kumar G,Hara H,Long C,Shaikh H,Ayares D,Cooper DK,Ezzelarab M

    更新日期：2012-04-01 00:00:00

  • Human umbilical cord mesenchymal stromal cells mitigate chemotherapy-associated tissue injury in a pre-clinical mouse model.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been shown to be a promising candidate for tissue regeneration and cancer therapy. However, their therapeutic potential against chemotherapy-induced side-effects remains unclear. METHODS:We treated murine Lewis lung carcinoma (LLC) and xenograft human colon tumors w...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.646044

    authors： Di GH,Jiang S,Li FQ,Sun JZ,Wu CT,Hu X,Duan HF

    更新日期：2012-04-01 00:00:00

  • Intravenous infusion of adipose-derived stem/stromal cells improves functional recovery of rats with spinal cord injury.

    abstract：BACKGROUND AIMS:Adipose tissue has therapeutic potential for spinal cord injury (SCI) because it contains multipotent cells known as adipose-derived stem/stromal cells (ASCs). In this study, we attempted intravenous ASC transplantation in rats with SCI to examine the effect on functional recovery. METHODS:ASCs (2.5 × ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2017.04.002

    authors： Ohta Y,Hamaguchi A,Ootaki M,Watanabe M,Takeba Y,Iiri T,Matsumoto N,Takenaga M

    更新日期：2017-07-01 00:00:00

  • Secretomes from bone marrow-derived mesenchymal stromal cells enhance periodontal tissue regeneration.

    abstract：BACKGROUND AIMS:Periodontal tissue regeneration with the use of mesenchymal stromal cells (MSCs) has been regarded as a future cell-based therapy. However, low survival rates and the potential tumorigenicity of implanted MSCs could undermine the efficacy of cell-based therapy. The use of conditioned media from MSCs (MS...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.11.009

    authors： Kawai T,Katagiri W,Osugi M,Sugimura Y,Hibi H,Ueda M

    更新日期：2015-04-01 00:00:00

  • Adipose mesenchymal stromal cells minimize and repair radiation-induced oral mucositis.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been used to minimize and repair radiation-induced normal tissue injury in the intestine, salivary gland, liver, skin, lungs and cardiac muscle. This study investigated the ability of adipose tissue-derived MSCs (aMSCs) to minimize and/or repair single dose radiatio...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2016.06.008

    authors： Maria OM,Shalaby M,Syme A,Eliopoulos N,Muanza T

    更新日期：2016-09-01 00:00:00

  • Improving efficacy of cancer immunotherapy by genetic modification of natural killer cells.

    abstract：:Natural killer (NK) cells are members of the innate immune system that recognize target cells via activating and inhibitory signals received through cell receptors. Derived from the lymphoid lineage, NK cells are able to produce cytokines and exert a cytotoxic effect on viral infected and malignant cells. It is their ...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2016.05.018

    authors： Burga RA,Nguyen T,Zulovich J,Madonna S,Ylisastigui L,Fernandes R,Yvon E

    更新日期：2016-11-01 00:00:00

  • Current evidence on mesenchymal stem cell therapy for traumatic spinal cord injury: systematic review and meta-analysis.

    abstract：BACKGROUND AIMS:The authors aim to analyze the evidence in the literature regarding the efficacy and safety of mesenchymal stem cell (MSC) therapy in human subjects with traumatic spinal cord injury (SCI) and identify its potential role in the management of SCI. METHODS:The authors conducted independent and duplicate ...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2020.09.007

    authors： Muthu S,Jeyaraman M,Gulati A,Arora A

    更新日期：2020-11-09 00:00:00

  • Adipose tissue-derived mesenchymal stem cell yield and growth characteristics are affected by the tissue-harvesting procedure.

    abstract：BACKGROUND:Adipose tissue contains a stromal vascular fraction that can be easily isolated and provides a rich source of adipose tissue-derived mesenchymal stem cells (ASC). These ASC are a potential source of cells for tissue engineering. We studied whether the yield and growth characteristics of ASC were affected by ...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240600621125

    authors： Oedayrajsingh-Varma MJ,van Ham SM,Knippenberg M,Helder MN,Klein-Nulend J,Schouten TE,Ritt MJ,van Milligen FJ

    更新日期：2006-01-01 00:00:00

  • A study of the immune properties of human umbilical cord lining epithelial cells.

    abstract：BACKGROUND AIMS:Our previous study has demonstrated the stem cell-like properties of human umbilical cord lining epithelial cells (CLECs) and their capability for epidermal reconstitution in organotypic skin culture; however, the immunogenicity of these cells has not been clearly defined. We assessed several aspects of...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.10.008

    authors： Cai YJ,Huang L,Leung TY,Burd A

    更新日期：2014-05-01 00:00:00

  • Hypoxia-inducible factor 1Α may regulate the commitment of mesenchymal stromal cells toward angio-osteogenesis by mirna-675-5P.

    abstract：BACKGROUND AIMS:During bone formation, angiogenesis and osteogenesis are regulated by hypoxia, which is able to induce blood vessel formation, as well as recruit and differentiate human mesenchymal stromal cells (hMSCs). The molecular mechanisms involved in HIF-1α response and hMSC differentiation during bone formation...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2017.09.007

    authors： Costa V,Raimondi L,Conigliaro A,Salamanna F,Carina V,De Luca A,Bellavia D,Alessandro R,Fini M,Giavaresi G

    更新日期：2017-12-01 00:00:00

  • Highly enriched cardiomyocytes from human embryonic stem cells.

    abstract：BACKGROUND:Current efforts to direct differentiation of human embryonic stem cells (hESC) into a particular cell lineage usually lead to a heterogeneous cell population with only a fraction of the desired cell type present. We show the generation of an essentially pure population of human cardiomyocytes from hESC using...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240802105307

    authors： Xu XQ,Zweigerdt R,Soo SY,Ngoh ZX,Tham SC,Wang ST,Graichen R,Davidson B,Colman A,Sun W

    更新日期：2008-01-01 00:00:00

  • Effects of stem cells on non-ischemic cardiomyopathy: a systematic review and meta-analysis of randomized controlled trials.

    abstract：BACKGROUND AIMS:To assess the impacts of stem cell therapy on clinical outcomes in patients with non-ischemic cardiomyopathy (NICM). The effect of stem cell therapy on prognosis is unclear and controversial. METHODS:The authors performed a systematic review and meta-analysis of the effects of autologous stem cell tran...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1016/j.jcyt.2020.06.006

    authors： Xia L,Zeng L,Pan J,Ding Y

    更新日期：2020-12-01 00:00:00

  • Expanded umbilical cord blood T cells used as donor lymphocyte infusions after umbilical cord blood transplantation.

    abstract：BACKGROUND:Umbilical cord blood (UCB) is an alternative graft source for hematopoietic stem cell transplantation and has been shown to give results comparable to transplantation with other stem cell sources. Donor lymphocyte infusion (DLI) is an effective treatment for relapsed malignancies after hematopoietic stem cel...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.08.001

    authors： Berglund S,Gertow J,Uhlin M,Mattsson J

    更新日期：2014-11-01 00:00:00

  • Effect of exposure to interleukin-21 at various time points on human natural killer cell culture.

    abstract：BACKGROUND AIMS:Interleukin-21 (IL-21) can enhance the effector function of natural killer (NK) cells but also limits their proliferation when continuously combined with IL-2/IL-15. Paradoxically, membrane-bound (mb)-IL-21 has been shown to improve human NK cell proliferation when cultured with IL-2/mb-IL-15. To clarif...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.04.008

    authors： Lim DP,Jang YY,Kim S,Koh SS,Lee JJ,Kim JS,Thi Phan MT,Shin DJ,Shin MG,Lee SH,Yoon M,Kim SK,Yoon JH,Park MH,Cho D

    更新日期：2014-10-01 00:00:00

  • Recombinant expression of coagulation factor VIII in hepatic and non-hepatic cell lines stably transduced with third generation lentiviral vectors comprising the minimal factor VIII promoter.

    abstract：BACKGROUND:Lentiviral vectors have the capacity to transduce stably non-dividing, differentiated and undifferentiated cells of various tissues, including liver. To obtain high-level expression of transgenes, vectors often rely on viral promoters. However, recent data suggest that the supraphysiologic expression from ub...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240701656053

    authors： Picanço V,Heinz S,Bott D,Behrmann M,Covas DT,Seifried E,Tonn T

    更新日期：2007-01-01 00:00:00

  • Neuropeptides to replace serum in cryopreservation of mesenchymal stromal cells?

    abstract：BACKGROUND AIMS:The therapeutic potential of human mesenchymal stromal cells (MSCs) has generated considerable interest in a wide variety of areas. MSC banking is feasible, but the optimal technique of cryopreservation remains to be determined. METHODS:To reduce dimethyl sulfoxide (DMSO) concentration in cryopreservat...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2013.06.012

    authors： Briquet A,Halleux A,Lechanteur C,Beguin Y

    更新日期：2013-11-01 00:00:00

  • Labeling of mesenchymal stromal cells with iron oxide-poly(L-lactide) nanoparticles for magnetic resonance imaging: uptake, persistence, effects on cellular function and magnetic resonance imaging properties.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) are the focus of research in regenerative medicine aiming at the regulatory approval of these cells for specific indications. To cope with the regulatory requirements for somatic cell therapy, novel approaches that do not interfere with the natural behavior of the cells a...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2011.571246

    authors： Schmidtke-Schrezenmeier G,Urban M,Musyanovych A,Mailänder V,Rojewski M,Fekete N,Menard C,Deak E,Tarte K,Rasche V,Landfester K,Schrezenmeier H

    更新日期：2011-09-01 00:00:00

  • Adverse events after infusions of cryopreserved hematopoietic stem cells depend on non-mononuclear cells in the infused suspension and patient age.

    abstract：BACKGROUND:Adverse events (AE) represent a significant clinical problem after infusion of cryopreserved HPC. However, the factors playing a role in the pathogenesis have not yet been fully established. METHODS:We prospectively collected data on AE that occurred with 179 HPC infusions performed on patients affected wit...

    journal_title：Cytotherapy

    pub_type： 临床试验,杂志文章

    doi：10.1080/14653240701326756

    authors： Milone G,Mercurio S,Strano A,Leotta S,Pinto V,Battiato K,Coppoletta S,Murgano P,Farsaci B,Privitera A,Giustolisi R

    更新日期：2007-01-01 00:00:00

  • Clinical analysis of the treatment of spinal cord injury with umbilical cord mesenchymal stem cells.

    abstract：BACKGROUND AIMS:The purpose of this study was to observe the clinical effect and safety of umbilical cord mesenchymal stem cells (UC-MSCs) in treating spinal cord injury (SCI) by intrathecal injection. METHODS:From January 2008 to October 2010, we treated 22 patients with SCI with UC-MSCs by intrathecal injection; dos...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2012.09.005

    authors： Liu J,Han D,Wang Z,Xue M,Zhu L,Yan H,Zheng X,Guo Z,Wang H

    更新日期：2013-02-01 00:00:00

  • Suicide genes as safety switches in T lymphocytes.

    abstract：:Broader application of adoptive transfer of tumor-specific T-lymphocytes is accompanied by the need for effective suicide genes to ensure the safety of this cell-based therapy. In vivo elimination of T-lymphocytes expressing the herpes simplex virus-derived thymidine kinase gene has demonstrated the feasibility of thi...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1080/14653240310001497

    authors： Straathof KC,Spencer DM,Sutton RE,Rooney CM

    更新日期：2003-01-01 00:00:00

  • Good manufacturing practice-compliant validation and preparation of BM cells for the therapy of acute myocardial infarction.

    abstract：BACKGROUND:Intracoronary application of BM-derived cells for the treatment of acute myocardial infarction (AMI) is currently being studied intensively. Simultaneously, strict legal requirements surround the production of cells for clinical studies. Thus good manufacturing practice (GMP)-compliant collection and prepara...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240601052734

    authors： Griesel C,Heuft HG,Herrmann D,Franke A,Ladas D,Stiehler N,Stucki A,Braun M,Wollert KC,Meyer GP,Drexler H,Hertenstein B,Ganser A,Rüdinger W,Arseniev L

    更新日期：2007-01-01 00:00:00

  • The significance of CD14+ monocytes in peripheral blood stem cells for the treatment of rat liver cirrhosis.

    abstract：BACKGROUND AIMS:Circulating monocytes have been exploited as an important progenitor cell resource for hepatocytes in vitro and are instrumental in the removal of fibrosis. We investigated the significance of monocytes in peripheral blood stem cells (PBSC) for the treatment of liver cirrhosis. METHODS:Rat CD14+ monocy...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.3109/14653249.2010.515578

    authors： Wang J,Zhou X,Cui L,Yan L,Liang J,Cheng X,Qiao L,Shi Y,Han Z,Cao Y,Han Y,Fan D

    更新日期：2010-12-01 00:00:00

  • Immunological and clinical effects of post-transplant G-CSF versus placebo in T-cell replete allogeneic blood transplant patients: results from a randomized double-blind study.

    abstract：BACKGROUND:Immunological and clinical effects of post-transplant growth factor administration have not been well studied. This report describes the outcome and immune functions of a total of 50 HLA-matched related donor allogeneic blood stem-cell transplantation patients who received post-transplant G-CSF (10 microg/kg...

    journal_title：Cytotherapy

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1080/14653240310003648

    authors： Joshi SS,Bishop MR,Lynch JC,Tarantolo SR,Abhyankar S,Bierman PJ,Vose JM,Geller RB,McGuirk J,Foran J,Bociek RG,Hadi A,Day SD,Armitage JO,Kessinger A,Pavletic ZS

    更新日期：2003-01-01 00:00:00

  • Cytokine flow cytometry: multiparametric approach to immune function analysis.

    abstract：:More precise quantitation of cellular immune responses has become possible with the advent of single-cell assays of immune function, such as cytokine flow cytometry, enzyme-linked immunospot (ELISPOT), and MHC-peptide multimers. Cytokine flow cytometry is an attractive technique because it allows the detection of resp...

    journal_title：Cytotherapy

    pub_type： 杂志文章,评审

    doi：10.1080/14653240310000029

    authors： Ghanekar SA,Maecker HT

    更新日期：2003-01-01 00:00:00

  • CTLA-4 blockade by a human MAb enhances the capacity of AML-derived DC to induce T-cell responses against AML cells in an autologous culture system.

    abstract：BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. M...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1080/14653240500499507

    authors： Zhong RK,Loken M,Lane TA,Ball ED

    更新日期：2006-01-01 00:00:00

  • Genomic and functional comparison of mesenchymal stromal cells prepared using two isolation methods.

    abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSCs) have been applied to patients in cell therapy for various diseases. Recently, we introduced a novel MSC separation filter device which could yield approximately 2.5-fold more MSCs from bone marrow in a closed system compared with the conventional open density gradient ce...

    journal_title：Cytotherapy

    pub_type： 杂志文章

    doi：10.1016/j.jcyt.2014.10.013

    authors： Otsuru S,Hofmann TJ,Raman P,Olson TS,Guess AJ,Dominici M,Horwitz EM

    更新日期：2015-03-01 00:00:00