In vitro expression of erythropoietin by transfected human mesenchymal stromal cells.

abstract：BACKGROUND AIMS:The Quantum® Cell Expansion System (Quantum; Terumo BCT, Inc, Lakewood, CO, USA) is a novel hollow fiber-based device that automates and closes the cell culture process, reducing labor intensive tasks such as manual cell culture feeding and harvesting. The manual cell selection and expansion processes f...

journal_title：Cytotherapy

authors： Jones M,Varella-Garcia M,Skokan M,Bryce S,Schowinsky J,Peters R,Vang B,Brecheisen M,Startz T,Frank N,Nankervis B

更新日期：2013-11-01 00:00:00

abstract：BACKGROUND AIMS:The term "cerebral palsy" (CP) encompasses many syndromes that emerge from brain damage at early stages of ontogenesis and manifest as the inability to retain a normal body position or perform controlled movements. Existing methods of CP treatment, including various rehabilitation strategies and surgica...

journal_title：Cytotherapy

authors： Romanov YA,Tarakanov OP,Radaev SM,Dugina TN,Ryaskina SS,Darevskaya AN,Morozova YV,Khachatryan WA,Lebedev KE,Zotova NS,Burkova AS,Sukhikh GT,Smirnov VN

更新日期：2015-07-01 00:00:00

abstract：:The prognosis of patients with acute myeloid leukemia (AML) remains dismal, with a 5-year overall survival rate of only 5.2% for the continuously growing subgroup of AML patients older than 65 years. These patients are generally not considered eligible for intensive chemotherapy and/or allogeneic hematopoietic stem ce...

journal_title：Cytotherapy

authors： Anguille S,Willemen Y,Lion E,Smits EL,Berneman ZN

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:The use of myeloma Ag-loaded mature DC vaccines, cryopreserved in single-use aliquots, is an attractive immunotherapeutic strategy. In this study we investigated the retention of phenotype, viability and potency of DC vaccines after freezing and thawing. METHODS:Plastic-adherent monocytes, derived from a st...

journal_title：Cytotherapy

authors： Szmania S,Yi Q,Cottler-Fox M,Rosen NA,Freeman J,Kordsmeier BJ,Moreno A,Shi J,Barlogie B,Tricot G,van Rhee F

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND AIMS:Despite the availability of multiple treatment strategies, patients with gastric carcinoma (GC) have a dismal prognosis. The aim of this study was to evaluate the efficacy and safety of cellular immunotherapy (CIT) with the use of autologous natural killer cells, γδT cells and cytokine-induced killer ce...

journal_title：Cytotherapy

authors： Cui J,Li L,Wang C,Jin H,Yao C,Wang Y,Li D,Tian H,Niu C,Wang G,Han W,Xu J,Chen J,Li W

更新日期：2015-07-01 00:00:00

abstract：BACKGROUND AIMS:Stem cell therapy for liver diseases has recently emerged as a promising alternative to liver transplantation. Eligible cells should have an appropriate immunophenotype. The aim of the present study was to define the immunological profile of two human liver-derived mesenchymal stromal cell populations, ...

journal_title：Cytotherapy

authors： Raicevic G,Najar M,Najimi M,El Taghdouini A,van Grunsven LA,Sokal E,Toungouz M

更新日期：2015-02-01 00:00:00

abstract：BACKGROUND AIMS:Shipment of therapeutic somatic cells between a current good manufacturing practice (cGMP) facility and a clinic or between different cGMP facilities requires validated standard operating procedures (SOP). Under National Heart Lung & Blood Institute (NHLBI) sponsorship, the Production Assistance for Cel...

journal_title：Cytotherapy

authors： Whiteside TL,Griffin DL,Stanson J,Gooding W,McKenna D,Sumstad D,Kadidlo D,Gee A,Durett A,Lindblad R,Wood D,Styers D

更新日期：2011-02-01 00:00:00

abstract：:There is a widely held expectation of clinical advance with the development of gene and cell-based therapies (GCTs). Yet, establishing benefits and risks is highly uncertain. We examine differences in decision-making for GCT approval between jurisdictions by comparing regulatory assessment procedures in the United Sta...

journal_title：Cytotherapy

authors： Coppens DGM,de Wilde S,Guchelaar HJ,De Bruin ML,Leufkens HGM,Meij P,Hoekman J

更新日期：2018-06-01 00:00:00

abstract：:There is rapidly increasing interest in the use of magnetic resonance imaging (MRI) to track cell migration in vivo. Iron oxide MR contrast agents can be detected at micromolar concentrations of iron, and offer sufficient sensitivity for T2*-weighted imaging. Cellular MRI shows potential for assessing aspects of cardi...

journal_title：Cytotherapy

authors： Zhang Z,Mascheri N,Dharmakumar R,Li D

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:Bone marrow mononuclear cells (BM-MNCs) and bone marrow-derived mesenchymal stem stromal cells (BM-MSCs) could have therapeutic potential for numerous conditions, including ischemia-related injury. Cells transplanted intravascularly may become entrapped in the lungs, which potentially decreases their th...

journal_title：Cytotherapy

authors： Mäkelä T,Takalo R,Arvola O,Haapanen H,Yannopoulos F,Blanco R,Ahvenjärvi L,Kiviluoma K,Kerkelä E,Nystedt J,Juvonen T,Lehenkari P

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cells (MSC) have been studied intensively in regenerative medicine. However, their therapeutic potential against tumor formation and cancer metastasis is still unclear. The effects of transplantation of MSCs in early-stage of carcinogenesis, should be evaluated. METHODS:MSC isolated...

journal_title：Cytotherapy

authors： Sun B,Roh KH,Park JR,Lee SR,Park SB,Jung JW,Kang SK,Lee YS,Kang KS

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:Multiple studies have demonstrated that 'purging' of autografts with 4-hydroperoxycyclophosphamide (4HC) or the related compound mafosfamide (Mf), to eradicate residual leukemia, produces the best results associated with autologous blood and marrow transplantation for AML. However, 4HC purging results in pro...

journal_title：Cytotherapy

authors： McNiece I,Civin C,Harrington J,Kellner J,Malehorn M,Turney J,Barber J,Jones R

更新日期：2006-01-01 00:00:00

abstract：:For more than a decade, Ab conjugated to a radionuclide emitting particulate radiation has been used in the management of leukemia in an effort to deliver targeted doses of radiation to BM, spleen and other sites of disease, while sparing normal organs. This radioimmunotherapy (RIT) approach has been employed to achie...

journal_title：Cytotherapy

authors： Pagel JM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND AIMS:The authors aim to analyze the evidence in the literature regarding the efficacy and safety of mesenchymal stem cell (MSC) therapy in human subjects with traumatic spinal cord injury (SCI) and identify its potential role in the management of SCI. METHODS:The authors conducted independent and duplicate ...

journal_title：Cytotherapy

authors： Muthu S,Jeyaraman M,Gulati A,Arora A

更新日期：2020-11-09 00:00:00

abstract：BACKGROUND AIMS:Mesenchymal stromal cell (MSC) transplantation has immerged as promising therapeutic approach to treat spinal cord injury (SCI). In this pilot study, we investigated the safety of intrathecal injection of autologous bone marrow-derived MSCs in nine patients with SCI. METHODS:Patients with complete SCI ...

journal_title：Cytotherapy

authors： Satti HS,Waheed A,Ahmed P,Ahmed K,Akram Z,Aziz T,Satti TM,Shahbaz N,Khan MA,Malik SA

更新日期：2016-04-01 00:00:00

abstract：BACKGROUND AIMS:Umbilical cord blood (UCB) is a rich source of stem cells, the characterization and isolation of which requires specific stem cell markers and reliable and reproducible protocols. METHODS:We assessed CD133 isolation in 39 UCB samples, using a commercial immunomagnetic cell-sorting protocol, and, becaus...

journal_title：Cytotherapy

authors： Pelagiadis I,Relakis K,Kalmanti L,Dimitriou H

更新日期：2012-07-01 00:00:00

abstract：BACKGROUND:The efficiency of chimeric antigen receptor (CAR) T-cell-based therapies depends on a sufficient expansion of CAR T cells in vivo and can be weakened by intra-tumoral suppression of CAR T cell functions, leading to a failure of therapy. For example, certain B-cell malignancies such as chronic lymphocytic leu...

journal_title：Cytotherapy

authors： Štach M,Ptáčková P,Mucha M,Musil J,Klener P,Otáhal P

更新日期：2020-12-01 00:00:00

abstract：BACKGROUND:Tumor cell lines have enormous value for the study of different aspects of cancer biology and have also recently gained great importance in autologous cell-based anti-tumor therapies. However, the use of these cells is still limited because in vitro growth is hampered by suboptimal culture conditions and cur...

journal_title：Cytotherapy

authors： Stadler G,Wieser M,Steindl F,Grillari J,Katinger H,Pfragner R,Voglauer R

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Cells from AML patients can differentiate into the phenotype of DC when cultured with GM-CSF and IL-4. Such cytokine-treated AML-derived DC (AML-DC) can stimulate autologous T cells. In this study we examined whether an anti-CTLA-4 MAb (MDX-010) could enhance the generation of autologous anti-AML T cells. M...

journal_title：Cytotherapy

authors： Zhong RK,Loken M,Lane TA,Ball ED

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND AIMS:Cartilage tissue engineering with human mesenchymal stromal cells (hMSC) is promising for allogeneic cell therapy. To achieve large-scale hMSC propagation, scalable microcarrier-based cultures are preferred over conventional static cultures on tissue culture plastic. Yet it remains unclear how microcarr...

journal_title：Cytotherapy

authors： Lin YM,Lim JF,Lee J,Choolani M,Chan JK,Reuveny S,Oh SK

更新日期：2016-06-01 00:00:00

abstract：BACKGROUND AIMS:Treatment of tendon-derived stem cells (TDSCs) with connective tissue growth factor (CTGF) and ascorbic acid promoted their tenogenic differentiation. We investigated the effects of TDSCs pre-treated with CTGF and ascorbic acid on tendon repair in a patellar tendon window injury rat model. METHODS:Gree...

journal_title：Cytotherapy

authors： Lui PP,Wong OT,Lee YW

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND AIMS:Virus-specific T-cell immunotherapy is emerging as a promising management strategy for virus infections in patients after hematopoietic stem cell transplant (HSCT). Here we present outcomes of 10 adult patients who received multi-virus-specific T cells prophylactically after HSCT. METHODS:Donor-derived...

journal_title：Cytotherapy

authors： Ma CK,Blyth E,Clancy L,Simms R,Burgess J,Brown R,Deo S,Micklethwaite KP,Gottlieb DJ

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND AND AIMS:We recently demonstrated that injection of conditioned medium (CM) generated from cells of the mesenchymal region of human amniotic membrane (AMTCs) reduces bleomycin-induced lung fibrosis in mice, suggesting a crucial role of paracrine factor(s) secreted by AMTCs in these beneficial effects. We fur...

journal_title：Cytotherapy

authors： Cargnoni A,Piccinelli EC,Ressel L,Rossi D,Magatti M,Toschi I,Cesari V,Albertini M,Mazzola S,Parolini O

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND AIMS:CD1d-restricted invariant natural killer (iNK) T cells are rare regulatory T cells that may contribute to the immune-regulation in allogeneic stem cell transplantation (ASCT). Here, we sought to develop an effective strategy to expand human iNK T cells for use in cell therapy to prevent graft-versus-hos...

journal_title：Cytotherapy

authors： Trujillo-Ocampo A,Cho HW,Herrmann AC,Ruiz-Vazquez W,Thornton AB,He H,Li D,Qazilbash MA,Ma Q,Porcelli SA,Shpall EJ,Molldrem J,Im JS

更新日期：2018-08-01 00:00:00

abstract：BACKGROUND AIMS:Enumeration of viable CD34(+) cells provides critical information for the bone marrow (BM) transplant physician. The single-platform ISHAGE protocol is the most reliable method currently available to quantitate accurately this important subset of cells. Previous studies have shown that 5 CD34(+) cells/m...

journal_title：Cytotherapy

authors： Sutherland DR,Nayyar R,Acton E,Giftakis A,Dean S,Mosiman VL

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND AIMS:Regenerative medicine strategies based on cell therapy are considered a promising approach to repair bone defects. The aims of this study were to evaluate the effect of subculturing on the osteogenic potential of osteoblasts derived from newborn rat calvaria and the effect of these osteoblasts on bone r...

journal_title：Cytotherapy

authors： Souza ATP,Freitas GP,Lopes HB,Ferraz EP,Oliveira FS,Beloti MM,Rosa AL

更新日期：2018-10-01 00:00:00

abstract：BACKGROUND AIMS:Specific and effective therapy for prevention or reversal of bronchiolitis obliterans (BO) is lacking. In this study, we evaluated the therapeutic effect of hepatocyte growth factor (HGF) gene modified mesenchymal stromal cells (MSCs) on BO. METHODS:A mouse model of experimental BO was established by s...

journal_title：Cytotherapy

authors： Cao XP,Han DM,Zhao L,Guo ZK,Xiao FJ,Zhang YK,Zhang XY,Wang LS,Wang HX,Wang H

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND AIMS:Stem cells derived from human adipose tissue (ASC) have the capacity for renewal, are easily obtained and have plasticity properties that allow them to differentiate into several cell types, including osteoblast cells. With the aim of understanding the issue of the osteogenic process and finding reliabl...

journal_title：Cytotherapy

authors： Giusta MS,Andrade H,Santos AV,Castanheira P,Lamana L,Pimenta AM,Goes AM

更新日期：2010-07-01 00:00:00

abstract：BACKGROUND AIMS:Research results have shown that bone mesenchymal stromal cells (BMSC) can different into neural cells. Electromagnetic fields (EMF) play a role in regulating cell proliferation and differentiation, but the mechanisms behind this are unknown. In the present study, we explored the efficacy of EMF on the ...

journal_title：Cytotherapy

authors： Bai WF,Xu WC,Feng Y,Huang H,Li XP,Deng CY,Zhang MS

更新日期：2013-08-01 00:00:00

abstract：:Kidney disease has reached epidemic proportions and is associated with high mortality and morbidity rates. Stem cell-based therapy may effectively treat kidney damage by cell transplantation. The breakthrough discovery using a combination of four transcription factors to reprogram genetically somatic cells into induce...

journal_title：Cytotherapy

authors： Wang WW,Wang W,Jiang Y,Han GF,Lu S,Li G,Zhang J

更新日期：2013-05-01 00:00:00