Abstract：
:Huge data accumulated in last few years have shown that differential expression of candidate miRNAs in normal versus transformed cell provides important insights into the pathogenesis of cancer including leukemias. In our previous report, we have revealed that miR-196b was significantly down-regulated in both EB-3 cells as well as B-cell ALL (acute lymphoblastic leukemia) patients as compared to their respective controls. We have unambiguously proven that miR-196b restoration in EB-3 cells leads to significant down-regulation of c-myc and its effector genes, i.e., human telomerase reverse transcriptase (hTERT), B-cell lymphoma/leukemia-2 (Bcl-2), apoptosis antagonizing transcription factor (AATF), and qualifies for tumor suppressor function in B-cell ALL. Keeping in view these results, the present study was aimed at dissecting the role of miR-196b and other miRNAs present near/within the genomic regions involved in genetic translocations characteristic of ALL in T-cell ALL cell lines and patient samples. We have demonstrated significant down-regulation in the expression of miR-196b in MOLT-4 and T-cell ALL patients with respect to the respective control cells. Transfection experiments revealed that none of the six identified miRNAs were able to knock down the expression of c-myc gene. Interestingly, it was found that miR-196b loses its ability to down-regulate c-myc gene expression in T-cell ALL as a consequence of mutations in target 3'-untranslated region (3'-UTR) of the c-myc gene. Results of the present study revealed that miR-196b becomes non-functional in T-cell ALL as a consequence of mutations in 3'-UTR of c-myc gene in T-cell ALL cellular models.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Bhatia S,Kaul D,Varma Ndoi
10.1007/s11010-010-0597-0subject
Has Abstractpub_date
2011-01-01 00:00:00pages
103-16issue
1-2eissn
0300-8177issn
1573-4919journal_volume
346pub_type
杂志文章abstract：:Acrolein is a highly reactive alpha, beta-unsaturated aldehyde, and a product of lipid peroxidation reactions. Acrolein is also an environmental pollutant and a key component of cigarette smoke, and has been implicated in multiple respiratory diseases. Lung tissue is a primary target for acrolein toxicity in smokers a...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9411-z
更新日期：2007-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1023/a:1024107512419
更新日期：2003-05-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00280273
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1019961922260
更新日期：2002-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1023/a:1006894527145
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doi：10.1007/s11010-006-9312-6
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doi：10.1023/a:1006890627639
更新日期：1997-04-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1007/BF00215300
更新日期：1980-03-20 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00240278
更新日期：1978-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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更新日期：2000-07-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00218133
更新日期：1990-02-09 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1012733719017
更新日期：2001-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006809202539
更新日期：1997-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：
更新日期：1997-09-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00219526
更新日期：1990-06-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01751307
更新日期：1975-12-31 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-020-03851-2
更新日期：2020-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-3089-7
更新日期：2017-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-009-0159-5
更新日期：2009-11-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00226230
更新日期：1979-01-26 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1386-8
更新日期：2012-10-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0384-y
更新日期：2010-03-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：
更新日期：1999-01-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0636-x
更新日期：2011-02-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2812-0
更新日期：2016-12-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1007042611127
更新日期：2000-01-01 00:00:00
abstract：:The effects of chloroperoxidase [EC 1.1.1.10] and hydrogen peroxide on the viabilities of quiescent and germinating conidiospores of an aflatoxigenic fungus, Aspergillus flavus, were determined. Hydrogen peroxide was found moderately lethal and chloroperoxidase produced a 30-fold increase in the lethality of hydrogen ...
journal_title：Molecular and cellular biochemistry
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更新日期：1999-05-01 00:00:00
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journal_title：Molecular and cellular biochemistry
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doi：10.1007/s11010-006-9137-3
更新日期：2006-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
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更新日期：1995-08-01 00:00:00
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journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：
更新日期：2003-09-01 00:00:00
