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Non-invasive quantitative 31P MRS assay of mitochondrial function in skeletal muscle in situ.

Abstract：

:A method for non-invasive, quantitative 31P NMR spectroscopic investigation of mitochondrial function in human skeletal muscle in situ is described. High time resolution 31P NMR measurements of phosphocreatine, inorganic phosphate and ATP resonances were conducted on human forearm flexor muscle during involuntary twitch contraction at eight different frequencies. Mitochondrial and glyco(geno)lytic ATP synthesis fluxes, and the cytosolic free energy of ATP hydrolysis (delta GP), were calculated at incremental steady-states of energy balance. The covariation of mitochondrial ATP synthesis flux, JPMOP, and delta GP was quasi-linear over the physiological range of free energy values. Curve-fit analysis of the covariation yielded a maximal sustainable JPMOP of 0.24 +/- 0.06 mmol ATP l-1.s-1 and a midpoint potential, (delta GP)0.5, of 58.1 +/- 1.2 kJ/mole in the muscle cells.

journal_name

Mol Cell Biochem

journal_title

Molecular and cellular biochemistry

authors

Jeneson JA,Wiseman RW,Kushmerick MJ

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

17-22

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

174

pub_type

杂志文章

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