Abstract：
:This study investigated the anticancer effects of embelin in human gastric cancer cells and the underlying molecular mechanisms. Gastric cancer cells were treated with embelin and 5-FU for methyl-thiazolyl-tetrazolium bromide cell viability assay and flow cytometric detection of cell viability and apoptosis. Protein pathway array (PPA) and Western blot were used to investigate differentially expressed proteins in embelin-treated gastric cancer cells. Embelin reduced gastric cancer cell viability, induced apoptosis, and enhanced 5-FU antitumor activity in gastric cancer cells. Mechanistically, embelin induced cell cycle arrest at the S and G2/M phases. Molecularly, embelin downregulated expression of X-linked inhibitor of apoptosis and cell cycle-regulatory proteins, such as CDK1, CDC25B, CDC25C, cyclinB1, and CDK2. PPA analysis showed that embelin modulated several pathways that are associated with cell growth and apoptosis, such as PI3K/AKT, JAK/STAT, p38 MAPK, and p53. The data from the current study implied that reduction of gastric cancer cell viability after treatment with embelin was through cell cycle arrest at the S and G2/M phases and apoptosis.
journal_name
Mol Cell Biochemjournal_title
Molecular and cellular biochemistryauthors
Wang DG,Sun YB,Ye F,Li W,Kharbuja P,Gao L,Zhang DY,Suo Jdoi
10.1007/s11010-013-1853-xsubject
Has Abstractpub_date
2014-01-01 00:00:00pages
143-52issue
1-2eissn
0300-8177issn
1573-4919journal_volume
386pub_type
杂志文章abstract：:Cardiac hypertrophy in rats was produced by aortic banding for 6 weeks and regression of hypertrophy in these experimental animals was induced by administration of angiotensin converting enzyme inhibitor, enalapril (10 mg/kg/day) for 6 weeks. The left ventricular muscle mass and systolic pressure were decrease upon tr...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00926849
更新日期：1993-02-17 00:00:00
abstract：:The PI-3 kinase signalling pathway is an important pathway in mediating the glucoregulatory effects of insulin and skeletal muscle (SKM) is the major tissue involved in glucose utilization. In diabetes this pathway is impaired, either due to lack of insulin as in Type I diabetes, or due to insulin resistance as in Typ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1017913130401
更新日期：2001-07-01 00:00:00
abstract：:The results of our experiments demonstrated that one hour of ischemia followed by one hour of reflow in the kidney caused a reduction in (Na+K+)ATPase activity and microsomal sulfhydryl content as well as an increase in microsomal lipid peroxidation. Renal venous malondialdehyde concentration was increased soon after ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00229689
更新日期：1987-12-01 00:00:00
abstract：:Protein kinase CK2 is a highly conserved and ubiquitous serine-threonine kinase. It is a tetrameric enzyme that is made up of two regulatory CK2beta subunits and two catalytic subunits, either CK2alpha/CK2alpha, CK2alpha/CK2alpha', or CK2alpha'/CK2alpha'. Although the two catalytic subunits diverge in their C termini,...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9811-8
更新日期：2008-09-01 00:00:00
abstract：:Doxorubicin (Dox) is an effective anti-cancer drug with severe reported cardiotoxicity. Cardiovascular risks associated with present cancer therapeutics demand urgent attention. There has been a growing interest in naturally occurring compounds to improve the therapeutic index as well as prevent non-tumour tissues fro...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-017-3195-6
更新日期：2018-05-01 00:00:00
abstract：:Previous studies reported that estrogen receptor β (ERβ) is localized to mitochondria, whereas little is known about the physiological functions of mitochondrial ERβ. In the present study, we explored the role of mitochondrial ERβ in regulating apoptosis using stable ERβ-expressing and ERβ knockdown cells lines. We fo...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2293-y
更新日期：2015-03-01 00:00:00
abstract：:Insulin receptor binding was examined in the microvillous membranes of mid-term (20-22 weeks of gestation, MT) and full-term (FT) placentas from patients with gestational diabetes mellitus (GDM) and in normal pregnant control (N). Mid-term placentas were obtained from patients who have had spontaneous abortion. The ma...
journal_title：Molecular and cellular biochemistry
pub_type： 临床试验,杂志文章
doi：10.1007/BF01076892
更新日期：1995-10-04 00:00:00
abstract：:Proton pump inhibitors (PPIs) are widely used to treat hyperacid secretion and stomach ulcers. The study investigated the anti-secretory and anti-ulcer effects of esomeprazole, the S-isomer of omeprazole on dimaprit, histamine and dibutyryl adenosine 3, 5 cyclic monophosphate (dbcAMP)-evoked gastric acid secretion, ac...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-007-9657-5
更新日期：2008-02-01 00:00:00
abstract：:Highly sensitive and specific synthetic substrates were used to quantitate cathepsin B and D activity in peritoneal macrophages in response to stimulation in vivo with mineral oil and thioglycollate. After intraperitoneal instillation of mineral oil the activity of cathepsin B increased significantly (to 15 300 units/...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00225928
更新日期：1985-11-01 00:00:00
abstract：:Caffeic acid (3,4-dihydroxy cinnamic acid) (CA) is naturally found in fruits, vegetables, olive oil, and coffee. This study was undertaken to evaluate the anticancer effect of caffeic acid on HT-1080 human fibrosarcoma cell line. The antiproliferative effect of caffeic acid was determined by MTT assay, and the oxidati...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-010-0655-7
更新日期：2011-03-01 00:00:00
abstract：:Accumulating data have shown that microRNAs are involved in the pathogenesis of cancer. miR-202 has been confirmed to be downregulated in several types of human cancer. However, the expression and biological role of miR-202 in osteosarcoma (OS) carcinogenesis and progression remain unclear. In this study, we demonstra...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-014-2195-z
更新日期：2014-12-01 00:00:00
abstract：:Gonolobus condurango plant extract is used as an anticancer drug in some traditional systems of medicine including homeopathy, but it apparently lacks any scientific validation. Further, no detailed study is available to suggest whether condurango-glycoside-A (CGA), a major ingredient of condurango serves as a potent ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-013-1732-5
更新日期：2013-10-01 00:00:00
abstract：:Zonation of ethanol oxidation and metabolic effects along the hepatic acini were investigated in the bivascularly perfused liver of fed rats. Ethanol was infused into the hepatic artery in antegrade and retrograde perfusion. Inhibition of glycolysis by ethanol, expressed as micromol min(-1) (ml accessible cell space)(...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/b:mcbi.0000012850.90719.6e
更新日期：2004-03-01 00:00:00
abstract：:Original studies with rat adrenocortical carcinoma identified a 180 kDa cell-surface protein which contained both guanylate cyclase and atrial natriuretic factor (ANF) receptor, representing a potentially new type of bifunctional receptor protein. It is both a receptor and a guanylate cyclase. This critical conclusion...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00230806
更新日期：1991-01-16 00:00:00
abstract：:Hydrogen sulfide (H2S) has been investigated widely in recent years. H2S plays a variety of roles in different biological systems, including cardiovascular system. It is the final product of amino acids metabolism, which contains sulfur-cysteine and homocysteine (Hcy). In human plasma, there are several various forms ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-015-2364-8
更新日期：2015-06-01 00:00:00
abstract：:Cardiosphere-derived cells (CDCs) and bone marrow mesenchymal stem cells (MSCs) are popularly used in stem cell therapy for myocardial regeneration. The cell type that survives and maintains stem cell characteristics in the adverse microenvironment following ischemia-reperfusion injury is presumed to be ideal for tran...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-016-2869-9
更新日期：2017-01-01 00:00:00
abstract：:The present study was aimed at addressing the effect of hyperglycemia on antioxidant enzymes. The expression of catalase, superoxide dismutase and glutathione peroxidase, the three primary scavenger enzymes involved in detoxifying reactive oxygen species has been evaluated in the renal cortex of rats after 6 weeks of ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1021620414979
更新日期：2003-01-01 00:00:00
abstract：:The antioxidant activity of anthocyanins has been well characterized in vitro; many cases has been postulated to provide an important exogenous mediator of oxidative stress in the gastrointestinal tract. The objective of this study was to evaluate the efficacy of anthocyanin protection against peroxyl radical (AAPH)-i...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-008-9729-1
更新日期：2008-05-01 00:00:00
abstract：:A simple method is described for the isolation of crystalline pyruvate kinase from human skeletal muscle. The enzyme was purified by ammonium sulfate fractionation, heat treatment and crystallization. Two crystal forms of pyruvate kinase differing in solubility but not in specific activity were found. The homogenous e...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01732078
更新日期：1975-03-27 00:00:00
abstract：:Two preparations with arginyl-tRNA synthetase activity have been obtained from rabbit liver post-microsomal fraction: a) a high-molecular-weight containing the multienzyme aminoacyl-tRNA synthetase complex and b) a low-molecular-weight preparation containing free enzymes. Thermal inactivation of arginyl-tRNA synthetas...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00222612
更新日期：1989-04-11 00:00:00
abstract：:Muscle injury is a common result of muscle exertion caused by overload and over-activity. In this presentation, an attempt was made to discuss models of muscle injury which involve exertion but not excessive strain, although most functional activities of the extremities require some eccentric muscle actions. Muscle in...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章,评审
doi：10.1023/a:1006832509488
更新日期：1998-02-01 00:00:00
abstract：:Hypertension and related oxidative stress are involved in the pathogenesis of any renal diseases. Angiotensin-converting enzyme inhibitors have multi-directional renoprotective effects. In this study, we aimed to investigate whether lisinopril treatment has any biochemical alterations on renal tissue in L-NAME (Nε-nit...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0760-2
更新日期：2011-06-01 00:00:00
abstract：:Five isoforms of CK2 may exist simultaneously in yeast cells: free catalytic subunits CK2α', CK2α and three holoenzymatic structures composed of αα'ββ', α(2)ββ' and α'(2)ββ'. Each isolated and purified form exhibits properties typical for CK2, but they differ in substrate specificity as well as in sensitivity to speci...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-011-0951-x
更新日期：2011-10-01 00:00:00
abstract：:Mature uteroferrin (Uf; Mr = 35,500) is a progesterone-induced acid phosphatase secreted by the pig uterus. It contains a single, unphosphorylated, high mannose-type oligosaccharide. Endometrial explants cultured in vitro secrete Uf with a Mr of 37,000 (37k Uf) having phosphorylated high mannose oligosaccharides. In t...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00227750
更新日期：1991-07-10 00:00:00
abstract：:Prolonged ischemia-reperfusion results in various damages in skeletal muscle. Following reperfusion, although the damaged muscles undergo regeneration, the precise process and mechanism of regeneration have not yet been fully understood. Here, we show the altered levels of plasma biochemical markers of muscle damage, ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/s11010-012-1470-0
更新日期：2013-01-01 00:00:00
abstract：:Visceral leishmaniasis has been found to be associated with severe anemia and premature lysis of erythrocytes. Peroxidative damage of red cells has been noted in several hemolytic anemias. Present study shows enhanced formation of methemoglobin in hamsters infected with Leishmania donovani. Increased formation of malo...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00944601
更新日期：1995-05-24 00:00:00
abstract：:The Arrhenius plots for the active and low activity soluble forms of the ATPase purified from the membranes of Micrococcus lysodeikticus grown at 30 degrees C presented discontinuities at 30 and 33 degrees C, respectively. Their activation parameters differed, being highest for the low activity form of the enzyme. Bot...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF01732550
更新日期：1977-08-19 00:00:00
abstract：:The effects of purified Ca2+, phospholipid-dependent protein kinase (C-kinase) were studied on adenylate cyclase activity from rat brain striatum. C-kinase treatment of the membranes stimulated adenylate cyclase activity, the maximal stimulation between 50-80% was observed at 3.5 U/ml, whereas the catalytic subunit of...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1007/BF00220724
更新日期：1990-01-18 00:00:00
abstract：:The effect of anti-regucalcin monoclonal antibody on neutral phoshatase activity in rat liver cytosol was investigated. Phosphotyrosine, phosphoserine, and phosphothreonine were used as the substrate toward phosphatase assay. Liver cytosolic phosphatase activity with three phosphoaminoacids was significantly increased...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006979606225
更新日期：1999-07-01 00:00:00
abstract：:The effects of chloroperoxidase [EC 1.1.1.10] and hydrogen peroxide on the viabilities of quiescent and germinating conidiospores of an aflatoxigenic fungus, Aspergillus flavus, were determined. Hydrogen peroxide was found moderately lethal and chloroperoxidase produced a 30-fold increase in the lethality of hydrogen ...
journal_title：Molecular and cellular biochemistry
pub_type： 杂志文章
doi：10.1023/a:1006905210810
更新日期：1999-05-01 00:00:00
