Gene targeting of CK2 catalytic subunits.

Abstract:

:Protein kinase CK2 is a highly conserved and ubiquitous serine-threonine kinase. It is a tetrameric enzyme that is made up of two regulatory CK2beta subunits and two catalytic subunits, either CK2alpha/CK2alpha, CK2alpha/CK2alpha', or CK2alpha'/CK2alpha'. Although the two catalytic subunits diverge in their C termini, their enzymatic activities are similar. To identify the specific function of the two catalytic subunits in development, we have deleted them individually from the mouse genome by homologous recombination. We have previously reported that CK2alpha' is essential for male germ cell development, and we now demonstrate that CK2alpha has an essential role in embryogenesis, as mice lacking CK2alpha die in mid-embryogenesis, with cardiac and neural tube defects.

journal_name

Mol Cell Biochem

authors

Seldin DC,Lou DY,Toselli P,Landesman-Bollag E,Dominguez I

doi

10.1007/s11010-008-9811-8

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

141-7

issue

1-2

eissn

0300-8177

issn

1573-4919

journal_volume

316

pub_type

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