Abstract：
:Workflows in urinary proteomics studies are often complex and require many steps to enrich, purify, deplete, and separate the complex mixture. Many of these methods are laborious, are time-consuming, and have the potential for error. Although individual steps of these methods have been previously studied, their downstream compatibilities with fractionation technologies such as off-gel electrophoresis have not been investigated. We developed a one-step sample preparation workflow that simultaneously (i) concentrates proteins, (ii) purifies by removing salts and other low molecular weight compounds, and (iii) depletes (albumin) from urine samples. This simple and robust workflow can be multiplexed and is compatible with a diverse range of downstream multidimensional separation technologies. Additionally, because of its high reproducibility and flexibility in processing samples with different volumes and concentrations, it has the potential to be used for standardization of urinary proteomics studies, as well as for studying other body fluids of similar complexity.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Vaezzadeh AR,Briscoe AC,Steen H,Lee RSdoi
10.1021/pr100924ssubject
Has Abstractpub_date
2010-11-05 00:00:00pages
6082-9issue
11eissn
1535-3893issn
1535-3907journal_volume
9pub_type
杂志文章abstract：:Tropomyosins (Tm) constitute a family of ubiquitous and highly conserved actin-binding proteins, playing essential roles in a variety of biological processes. Tm isoforms produced by multiple Tm encoding genes and alternatively expressed exons along with post-translational modifications (PTMs) regulate Tm function. Th...
journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2012-06-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00255
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pub_type： 杂志文章
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2013-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2010-04-05 00:00:00
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journal_title：Journal of proteome research
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更新日期：2015-02-06 00:00:00
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journal_title：Journal of proteome research
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