Abstract:
:We report the stimulation of neurogenesis and myogenesis of human mesenchymal stem cells (hMSCs) on the surfaces of biodegradable hydrogels with different stiffness. The hydrogels were composed of gelatin-hydroxyphenylpropionic acid (Gtn-HPA) conjugate were formed using the oxidative coupling of phenol moieties catalyzed by hydrogen peroxide (H(2)O(2)) and horseradish peroxidase (HRP). The storage modulus of the hydrogels was readily tuned from 600 to 12800 Pa. It was found that the stiffness of the hydrogel strongly affected the cell attachment, focal adhesion, migration and proliferation rate of hMSCs. The hMSCs on stiffer surfaces have a larger spreading area, more organized cytoskeletons, more stable focal adhesion, faster migration and a higher proliferation rate. The gene expression related to the extracellular matrix and adhesion molecules also differed when the cells were cultured on hydrogels with different stiffness. The differentiation of hMSCs on the surface of the hydrogel was closely linked to the hydrogel stiffness. The cells on a softer hydrogel (600 Pa) expressed more neurogenic protein markers, while cells on a stiffer hydrogel (12000 Pa) showed a higher up-regulation of myogenic protein markers.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Wang LS,Boulaire J,Chan PP,Chung JE,Kurisawa Mdoi
10.1016/j.biomaterials.2010.07.075subject
Has Abstractpub_date
2010-11-01 00:00:00pages
8608-16issue
33eissn
0142-9612issn
1878-5905pii
S0142-9612(10)00947-6journal_volume
31pub_type
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